Standard name
Human Ortholog
Description Phosphoserine phosphatase of the phosphoglycerate pathway; involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 1.0 1.0 0.98 0.98 0.96 0.89 0.84 0.9 0.88 0.89 0.83 0.99 0.99 0.99 1.0 0.99 0.95 0.96 0.94 0.92 0.97 0.97 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.11 0.06 0.06 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 2 2 2 2 5 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 2 1 3 2 1 1 1 0 1 0 0 0 0 0 0 0 4 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 5 10 11 16 25 19 20 4 17 2 2 8 1 2 4 0 0 0 0 0 0
Cytoplasm 120 70 69 351 537 442 591 605 446 434 329 401 490 713 799 73 145 97 116 70 91 254 587 624
Endoplasmic Reticulum 1 0 0 1 5 13 52 77 28 31 9 32 3 4 1 1 4 2 0 0 0 0 3 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 1 1 0 1 1 0 0 1 0 0 0 1 0 0 0 0 0 0
Nucleus 0 0 0 4 1 5 10 14 5 11 3 8 0 0 0 0 0 0 0 1 1 2 2 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 2 5 2 12 17 12 16 25 45 1 0 2 1 1 2 0 2 1 0 1 6
Unique Cell Count 122 70 69 358 549 460 667 718 498 496 368 484 494 717 804 73 147 102 121 75 99 262 608 647
Labelled Cell Count 122 70 69 363 559 473 687 742 516 517 374 509 497 722 812 76 153 107 121 75 99 262 608 647


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 32.2 25.5 33.5 33.1 30.6 25.2 22.7 22.9 20.4 19.9 18.1 18.6 41.1 44.0 40.5 56.1 57.8 52.2 35.7 36.8 37.9
Std Deviation (1e-4) 6.1 9.7 7.2 6.6 5.7 4.3 4.2 4.4 4.0 4.0 3.7 3.8 7.9 9.8 9.0 15.5 17.5 19.5 6.9 8.3 8.6
Intensity Change (Log2) – – – -0.02 -0.13 -0.41 -0.56 -0.55 -0.71 -0.75 -0.89 -0.85 0.3 0.39 0.27 0.74 0.79 0.64 0.09 0.13 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.2 -1.2 -1.7 -3.0 -3.6 -2.8 -3.1 -2.8 -3.7 -0.8 -0.6 -0.7 0 -1.0 -1.9
Endoplasmic Reticulum 0 0 0 0 2.9 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 2.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 28.4441 33.9015 31.0926 29.3493 31.4311 31.521 26.1088 37.6052 35.2668 36.2875 28.2837 35.4654 32.889 37.6705 35.7788 33.7177 34.7964 34.8447
Actin 0.0061 0.0001 0.0066 0.0001 0.0153 0.0002 0.0105 0.0001 0.0008 0.0001 0.0041 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0006
Bud 0.0003 0.0001 0.0005 0.0001 0.0001 0 0.0007 0 0.0006 0 0.0005 0.0001 0.0001 0.0011 0.0001 0 0 0.0003
Bud Neck 0.0004 0.0001 0.0005 0.0003 0.0005 0.0004 0.0013 0.0001 0.0002 0.0002 0.0006 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0006
Bud Periphery 0.0002 0 0.0004 0.0001 0.0003 0 0.0007 0 0.0007 0 0.0004 0 0 0.0002 0 0 0 0.0001
Bud Site 0.0003 0.0001 0.0003 0.0001 0.0003 0 0.0018 0 0.0003 0 0.0009 0 0.0001 0.0002 0 0 0 0
Cell Periphery 0.0001 0 0.0003 0.0002 0.0002 0 0.0002 0 0.0001 0 0.0002 0 0.0001 0 0 0 0 0
Cytoplasm 0.9258 0.99 0.9594 0.9387 0.9305 0.9687 0.8529 0.9947 0.9651 0.99 0.8597 0.9744 0.9575 0.9904 0.9851 0.9878 0.9913 0.9692
Cytoplasmic Foci 0.0072 0.0002 0.0031 0.0053 0.0006 0.0019 0.0158 0.0001 0.0014 0.0002 0.024 0.0005 0.0004 0.0004 0.0014 0.0001 0.0003 0.0011
Eisosomes 0.0001 0 0.0001 0 0.0005 0 0.0004 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0019 0.0003 0.0008 0.0011 0.0042 0.0017 0.0056 0.0001 0.0006 0.0002 0.0035 0.0007 0.0007 0.0003 0.0001 0.0001 0.0001 0.0009
Endosome 0.0026 0.0001 0.0023 0.0023 0.0105 0.0016 0.0157 0 0.0018 0 0.0031 0.0004 0.001 0.0001 0.0001 0 0.0001 0.0006
Golgi 0.0011 0 0.0003 0.0002 0.0039 0.0001 0.0019 0 0.0005 0 0.0021 0 0 0 0 0 0 0.0001
Lipid Particles 0.0031 0 0.0011 0.0055 0.0032 0.0005 0.0051 0 0.0006 0 0.0146 0 0 0 0 0 0 0
Mitochondria 0.0025 0.0001 0.0044 0.0027 0.0027 0.0001 0.0083 0 0.0113 0 0.0037 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0003
None 0.0023 0.0001 0.0009 0.0058 0.002 0.0003 0.0102 0.0001 0.0009 0.0001 0.0075 0.0002 0.002 0.0004 0.0005 0.0001 0.0001 0.0003
Nuclear Periphery 0.0056 0.0012 0.0015 0.0061 0.0088 0.0039 0.0161 0.0004 0.0013 0.0012 0.0095 0.0032 0.0077 0.001 0.0011 0.0014 0.0006 0.0047
Nucleolus 0.0028 0 0.0002 0.0027 0.0002 0 0.0028 0 0.0004 0 0.0017 0 0.0002 0 0 0 0 0
Nucleus 0.0273 0.0072 0.0113 0.0196 0.0129 0.018 0.0289 0.0043 0.0101 0.0078 0.0174 0.0187 0.0257 0.0051 0.0105 0.0102 0.0071 0.0202
Peroxisomes 0.0042 0 0.0003 0.0003 0.0003 0 0.0023 0 0.0003 0 0.0315 0 0 0 0 0 0 0
Punctate Nuclear 0.0037 0.0001 0.0004 0.0021 0.0007 0.0017 0.012 0 0.0005 0.0001 0.012 0.0002 0.0008 0.0001 0.0005 0 0 0.0002
Vacuole 0.0017 0.0002 0.0044 0.0037 0.0008 0.0005 0.0051 0 0.0018 0.0001 0.002 0.0008 0.0024 0.0003 0.0001 0.0001 0.0001 0.0005
Vacuole Periphery 0.0007 0 0.0008 0.0029 0.0012 0.0001 0.0017 0 0.0007 0 0.0009 0.0002 0.0008 0 0 0 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 63.4754 49.8491 40.1383 43.7563 54.6094 56.9184 58.1772 49.2178 41.5595 56.0132
Translational Efficiency 1.5063 1.7856 1.6697 1.2399 1.3555 1.489 1.5183 1.6713 1.741 1.6501

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phosphoserine phosphatase of the phosphoglycerate pathway; involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ser2

Ser2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ser2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available