Standard name
Human Ortholog
Description Beta subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.17 – 0 0.05 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.35 0.46 0.6 0.72 – 0 0.08 0 0.12 0.08 0.11
Mitochondria 0.96 0.95 0.95 0.96 0.89 0.92 0.94 0.92 0.93 0.94 0.96 0.94 0.85 0.78 0.81 0 0 – 0.85 0.79 0.81 0.73 0.72 0.72
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.05 0.05 0 0.05 0 0.07 0 0.06 0 0 0 – 0 0 0 0 0 0
Nucleolus 0.08 0.09 0.05 0 0.11 0.14 0.17 0.12 0.14 0.11 0.13 0.12 0.06 0 0 0.17 0.06 – 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0.07 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0 0 0 0 0 0 0 0 0 0 0 0.06 0.14 0.1 0.06 0.06 – 0 0 0 0.05 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 0 2 3 2 3 3 3 4 4 5 4 3 – 4 5 10 7 11 9
Bud 3 1 1 1 2 1 8 4 4 9 6 7 0 1 2 2 0 – 0 0 1 4 2 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 1 0 0 1 3 3 0 2 2 2 0 0 0 0 0 – – – – – – –
Cell Periphery 2 1 7 2 1 0 0 5 2 3 0 1 9 11 7 2 1 – 0 0 0 0 0 0
Cytoplasm 0 1 1 1 8 6 7 3 6 1 2 3 6 5 5 2 2 – 0 0 0 1 0 2
Endoplasmic Reticulum 0 0 0 1 0 0 2 4 0 4 2 1 1 0 3 2 1 – 0 0 0 0 1 1
Endosome 0 0 0 0 1 0 0 1 0 0 0 0 3 5 2 0 0 – 1 2 2 4 6 5
Golgi 2 3 7 3 0 1 2 2 6 1 0 1 63 152 218 29 13 – 12 8 11 39 32 47
Mitochondria 306 96 228 185 204 280 408 401 416 462 349 465 261 340 386 2 0 – 271 81 211 246 281 313
Nucleus 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0 0 – 0 0 0 2 2 2
Nuclear Periphery 14 1 4 3 5 11 23 20 14 23 9 34 13 24 11 0 0 – 0 0 1 2 2 6
Nucleolus 27 9 12 6 24 41 75 53 65 56 47 58 20 11 7 8 1 – 1 0 0 4 8 3
Peroxisomes 2 1 18 8 3 1 2 3 4 2 1 6 10 11 8 0 0 – 17 3 17 5 10 5
SpindlePole 0 0 2 0 0 0 0 1 0 1 1 1 2 0 0 0 0 – 1 0 0 0 4 1
Vac/Vac Membrane 25 4 3 2 6 5 4 12 9 12 8 9 18 59 47 3 1 – 3 0 0 15 20 20
Unique Cell Count 320 101 241 192 228 303 436 438 449 494 363 497 308 435 475 48 18 318 103 260 338 391 437
Labelled Cell Count 381 117 286 212 254 348 536 515 528 580 430 591 411 623 701 54 22 318 103 260 338 391 437


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.9 20.8 19.3 19.8 21.7 22.8 21.7 23.3 22.1 21.1 19.9 21.3 17.3 17.0 16.9 32.6 33.5 – 18.7 18.6 20.9
Std Deviation (1e-4) 3.7 6.0 4.7 4.8 5.3 6.9 6.7 7.8 7.5 7.1 7.2 7.6 3.9 3.6 3.4 12.3 10.3 – 4.7 6.0 6.1
Intensity Change (Log2) – – – 0.03 0.17 0.24 0.17 0.27 0.19 0.13 0.04 0.14 -0.16 -0.18 -0.19 0.75 0.79 – -0.04 -0.06 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 -0.3 0 0 0 –
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Golgi 0 0 0 0 0 0 0 0 0 6.1 9.4 11.7 11.0 0 –
Mitochondria 0.9 -2.1 -1.0 -0.5 -1.5 -1.0 -0.6 0.9 -0.6 -3.7 -5.6 -4.8 -14.2 -11.9 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nuclear Periphery 0 0 1.4 2.3 2.0 1.1 2.0 0.7 3.0 1.7 2.4 0.6 0 0 –
Nucleolus -1.0 2.3 3.3 4.6 3.0 3.8 2.8 3.2 2.9 0.8 -1.7 -2.8 0 0 –
Peroxisomes -1.4 -3.2 -4.5 -5.2 -4.9 -4.7 -5.5 -5.0 -4.5 -2.2 -3.0 -3.9 0 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Vacuole 0 0 0 0 1.3 0 0 0 0 2.8 5.3 4.3 0 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.172 10.5747 10.0178 9.4046 9.2245 9.8395 6.5729 8.0844 7.4546 7.3425 5.2007 7.0491 6.799 6.9115 6.4316 6.2646 5.9908 6.557
Actin 0.0266 0.0022 0.0116 0.002 0.0001 0.0073 0.0507 0.0075 0.0168 0.0088 0.0021 0.0038 0.0012 0.0015 0.0014 0.0191 0.0004 0.0008
Bud 0.0003 0.0002 0.0001 0.0001 0 0.0001 0.0006 0.0002 0.0003 0.0006 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0 0
Bud Neck 0.0002 0.0001 0.0001 0 0 0 0.004 0.0001 0.0003 0.0001 0.0004 0.0004 0.0001 0 0 0.0005 0 0.0001
Bud Periphery 0.0008 0.0006 0.0008 0.0001 0 0.0002 0.0012 0.0004 0.0004 0.0011 0.0002 0.0004 0.0001 0.0003 0.0002 0.0002 0 0.0001
Bud Site 0.0006 0.0007 0.0003 0.0003 0 0.0001 0.0076 0.0021 0.0013 0.001 0.0043 0.0002 0.0004 0.0015 0.0002 0.0012 0 0.0001
Cell Periphery 0.0001 0.0004 0.0007 0 0.0001 0.0001 0.0006 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0021 0 0 0 0 0 0.0026 0.0001 0.0002 0 0.0005 0.0022 0.0007 0 0 0.0002 0 0.0002
Cytoplasmic Foci 0.0159 0.0045 0.0031 0.0005 0.0001 0.0005 0.0096 0.0024 0.0043 0.0005 0.0111 0.002 0.0144 0.0013 0.0034 0.0103 0.0007 0.0019
Eisosomes 0.0006 0.0002 0.0008 0.0001 0.0001 0.0031 0.0006 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0005 0.0001 0.0001 0 0 0.0002 0.0022 0.0002 0.0006 0 0.0003 0.0038 0.0002 0.0001 0.0001 0.0002 0 0.0002
Endosome 0.0072 0.0025 0.002 0.0004 0.0002 0.0021 0.021 0.0122 0.0065 0.0005 0.0406 0.0054 0.0128 0.0032 0.0052 0.004 0.0011 0.004
Golgi 0.0164 0.0054 0.0027 0.0007 0.0006 0.009 0.0129 0.027 0.0133 0.0014 0.0113 0.0046 0.0104 0.0072 0.0162 0.0046 0.0024 0.0079
Lipid Particles 0.0287 0.023 0.0131 0.0013 0.0033 0.0031 0.027 0.0037 0.0088 0.0005 0.0153 0.0025 0.0155 0.0023 0.0043 0.006 0.0247 0.0083
Mitochondria 0.8505 0.8909 0.9347 0.9899 0.9928 0.9688 0.8103 0.9251 0.9346 0.9811 0.8939 0.9592 0.9248 0.9668 0.9607 0.9402 0.9612 0.9617
None 0.0097 0 0.0001 0 0 0.0002 0.0013 0 0.0003 0 0.0003 0.0004 0.0012 0 0 0.0004 0 0.0001
Nuclear Periphery 0.0086 0.0002 0.0001 0 0 0.0005 0.0026 0.0001 0.0012 0 0.0003 0.0068 0.001 0.0004 0.0002 0.0002 0 0.0032
Nucleolus 0.0007 0.0004 0.0004 0 0 0 0.0034 0.0001 0.0001 0 0.0006 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0008 0.0001 0.0001 0 0 0 0.0038 0.0001 0.0002 0 0.0004 0.0027 0.0003 0 0 0 0 0.0007
Peroxisomes 0.0171 0.0439 0.0164 0.0039 0.0008 0.0021 0.0282 0.0108 0.0073 0.0037 0.0112 0.0022 0.009 0.0106 0.0054 0.0111 0.0071 0.0065
Punctate Nuclear 0.0089 0 0.0001 0 0 0.0001 0.003 0 0.0004 0 0.0004 0.0004 0.002 0 0 0.0006 0 0.0006
Vacuole 0.0007 0.0044 0.0038 0.0002 0.0002 0.0003 0.0034 0.0021 0.001 0.0001 0.0048 0.0008 0.0017 0.0007 0.0006 0.0002 0.0005 0.0005
Vacuole Periphery 0.0031 0.0201 0.009 0.0004 0.0016 0.0023 0.0033 0.0052 0.0015 0.0003 0.0015 0.0018 0.0035 0.0035 0.0015 0.0004 0.0015 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 84.2162 66.8339 60.4244 62.6132 58.4947 83.3107 80.0034 82.0438 93.1778 69.4904
Translational Efficiency 1.5946 1.556 1.3079 1.5528 1.4408 1.8744 1.5219 1.5477 1.4158 1.5607

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2168 1141 1332 1233 2031 1470 162 2534 4199 2611 1494 3767

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1406.01 1511.09 2101.72 1965.37 1368.31 1462.22 2404.05 2258.04 1387.78 1483.58 2134.50 2162.24
Standard Deviation 285.66 408.55 486.03 529.92 299.08 317.72 531.36 524.58 292.83 361.06 500.06 543.96
Intensity Change Log 2 — 0.103983 0.579964 0.483194 — 0.095765 0.813072 0.722676 — 0.099936 0.699639 0.606274

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001646 0.007548 0.006730 0.005391 0.002257 0.003857 0.014431 0.004827 0.001941 0.005470 0.007565 0.005012
Bud Neck 0.000321 0.002268 0.002048 0.004936 0.000529 0.000735 0.012048 0.000239 0.000422 0.001405 0.003132 0.001776
Bud Site 0.000248 0.002812 0.001049 0.009671 0.000776 0.000663 0.007607 0.000430 0.000503 0.001602 0.001760 0.003455
Cell Periphery 0.000050 0.000218 0.000072 0.000491 0.000064 0.000066 0.000166 0.000168 0.000057 0.000133 0.000082 0.000274
Cytoplasm 0.000091 0.000905 0.000246 0.000579 0.000171 0.000207 0.000651 0.000117 0.000130 0.000512 0.000290 0.000268
Cytoplasmic Foci 0.005220 0.020168 0.021987 0.013415 0.006073 0.016843 0.073712 0.002102 0.005633 0.018296 0.027596 0.005805
Eisosomes 0.000684 0.001836 0.001116 0.000731 0.000852 0.000686 0.001495 0.001333 0.000765 0.001189 0.001157 0.001136
Endoplasmic Reticulum 0.000076 0.000175 0.000097 0.000428 0.000050 0.000127 0.000398 0.000041 0.000063 0.000148 0.000130 0.000167
Endosome 0.000471 0.003847 0.001324 0.005593 0.000719 0.002481 0.006149 0.000330 0.000591 0.003078 0.001847 0.002053
Golgi 0.005729 0.021815 0.022099 0.019639 0.007821 0.034942 0.027330 0.006835 0.006741 0.029206 0.022667 0.011026
Lipid Particles 0.003312 0.007882 0.008767 0.006360 0.002738 0.007885 0.006466 0.000589 0.003034 0.007884 0.008517 0.002478
Mitochondria 0.969628* 0.898942* 0.899485* 0.879118* 0.962235* 0.909233* 0.754482* 0.976810* 0.966052* 0.904736* 0.883762* 0.944834*
Mitotic Spindle 0.000174 0.001437 0.003162 0.003350 0.000087 0.000915 0.011081 0.000394 0.000132 0.001143 0.004021 0.001362
None 0.002197 0.002165 0.004118 0.003329 0.002243 0.001775 0.002865 0.001153 0.002219 0.001945 0.003982 0.001865
Nuclear Periphery 0.000099 0.000267 0.000848 0.001309 0.000122 0.000246 0.002363 0.000047 0.000110 0.000255 0.001013 0.000460
Nuclear Periphery Foci 0.000072 0.000316 0.000287 0.000782 0.000133 0.000168 0.001296 0.000019 0.000101 0.000233 0.000396 0.000269
Nucleolus 0.000180 0.001074 0.000921 0.001220 0.000272 0.000351 0.006883 0.000090 0.000225 0.000667 0.001568 0.000460
Nucleus 0.000052 0.000428 0.000059 0.000181 0.000025 0.000177 0.000740 0.000012 0.000039 0.000287 0.000133 0.000067
Peroxisomes 0.005869 0.018098 0.010157 0.004799 0.009471 0.009594 0.035561 0.002269 0.007611 0.013310 0.012911 0.003097
Vacuole 0.001320 0.004329 0.004786 0.015434 0.001046 0.004197 0.016501 0.000546 0.001188 0.004255 0.006056 0.005419
Vacuole Periphery 0.002561 0.003468 0.010641 0.023246 0.002315 0.004853 0.017774 0.001648 0.002442 0.004248 0.011415 0.008717

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.60 -4.67 -3.59 1.45 1.00 -2.06 -3.00 -3.33 -1.08 2.35 -4.92 -5.58 -4.90 0.55 2.37
Bud Neck -5.13 -6.55 -5.94 -3.08 -3.57 -0.86 -5.00 1.42 3.64 5.15 -4.76 -7.52 -4.90 -1.17 3.12
Bud Site -4.04 -4.62 -5.32 -3.67 -4.87 0.25 -3.26 0.85 0.88 3.47 -3.04 -3.80 -4.78 -2.82 -2.63
Cell Periphery -3.86 -1.44 -4.64 -2.66 -4.41 -0.16 -4.32 -3.25 -3.23 -0.03 -3.71 -2.00 -5.74 -3.39 -4.98
Cytoplasm -1.54 -3.75 -4.63 0.56 -3.30 -0.49 -3.93 0.72 1.86 4.82 -1.63 -3.58 -2.63 1.00 0.20
Cytoplasmic Foci -8.42 -8.36 -5.61 2.92 3.45 -7.43 -6.12 5.92 10.87 6.49 -11.29 -10.10 -0.42 10.59 9.86
Eisosomes -4.08 -5.13 -0.61 3.86 3.97 1.56 -1.98 -2.96 -4.26 0.47 -3.11 -4.52 -3.49 0.33 0.17
Endoplasmic Reticulum -3.46 -0.99 -2.83 -2.02 -2.67 -3.62 -3.11 0.98 4.12 3.19 -4.76 -3.44 -2.50 -0.46 -0.86
Endosome -5.57 -3.85 -5.81 -1.66 -4.74 -5.55 -3.44 2.71 7.16 3.69 -7.81 -4.76 -4.83 2.37 -0.54
Golgi -6.91 -7.75 -6.47 0.73 0.87 -9.45 -3.20 0.79 9.89 3.37 -11.79 -8.08 -4.08 9.04 5.62
Lipid Particles -3.51 -3.87 -2.75 0.96 1.47 -4.08 -2.46 5.13 6.07 4.01 -5.27 -4.60 1.04 6.01 5.12
Mitochondria 10.59 11.75 12.89 2.28 2.49 9.04 8.17 -4.64 -12.36 -8.77 13.98 13.71 6.92 -8.32 -9.66
Mitotic Spindle -1.89 -5.01 -3.43 -1.74 -0.27 -2.18 -3.74 -1.53 1.23 3.63 -2.80 -6.26 -3.69 -0.51 3.71
None 0.17 -6.06 -5.13 -4.84 2.21 3.04 -2.20 8.10 5.62 6.61 2.33 -6.32 3.15 0.69 7.64
Nuclear Periphery -4.36 -7.24 -6.10 -5.18 -2.06 -2.93 -4.92 2.30 7.18 5.09 -5.21 -8.52 -5.17 -2.96 4.48
Nuclear Periphery Foci -3.99 -6.18 -5.18 -3.16 -3.57 -0.45 -4.72 1.74 6.61 5.40 -2.93 -5.72 -2.99 -0.73 2.07
Nucleolus -3.12 -3.28 -4.32 -0.67 -1.22 -0.46 -2.84 2.05 1.74 2.92 -2.81 -4.12 -2.69 1.00 3.22
Nucleus -1.32 -0.10 -2.55 0.72 -2.80 -1.13 -1.50 2.24 1.22 1.53 -1.70 -1.67 -1.61 1.42 0.95
Peroxisomes -5.38 -2.49 0.67 6.16 3.38 -0.06 -3.69 5.54 4.76 4.76 -3.87 -3.24 4.98 8.06 6.69
Vacuole -4.89 -5.79 -8.48 -6.37 -6.15 -4.20 -5.23 2.67 4.96 5.41 -6.10 -7.98 -7.39 -1.59 0.79
Vacuole Periphery -1.63 -6.67 -10.20 -9.72 -5.47 -4.01 -4.91 1.74 5.63 5.14 -4.17 -8.07 -8.60 -5.80 2.11
Standard name
Human Ortholog
Description Beta subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate
Localization
Cell Percentages mitochondrion (100%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Lsc2

Lsc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lsc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available