Standard name
Human Ortholog
Description Subunit of the endosomal Vps27p-Hse1p complex; complex is required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.12 0.08 0.15 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0.06 0.13 0.12 0.14 0.1 0.14 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0.05 0 0 0 0.05 0.06 0.06 0.1 0.17 0.09 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0.77 0.58 0.76 0.45 0.58 0.43 0.31 0.4 0.39 0.28 0.22 0.23 0.84 0.82 0.75 0.51 0.55 0.39 0.88 0.81 0.8 0.85 0.85 0.84
Golgi 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.14 0.21 0 0 0 0 0 0.06
Mitochondria 0.18 0.16 0.05 0.27 0.21 0.38 0.37 0.28 0.37 0.44 0.57 0.5 0 0 0 0 0.09 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0.08 0.09 0.12 0.14 0.09 0.11 0.09 0.07 0.06 0.08 0.06 0 0 0 0 0 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0.23 0.32 0.22 0.2 0.21 0.2 0.13 0.16 0.12 0.12 0.09 0 0 0 0 0 0 0 0.06 0 0 0
SpindlePole 0.11 0.07 0.31 0.24 0.25 0.16 0.24 0.22 0.23 0.17 0.16 0.14 0.15 0.21 0.16 0.12 0.14 0.11 0 0 0.07 0 0 0
Vac/Vac Membrane 0.15 0.21 0 0 0 0 0 0 0 0 0 0 0.11 0.17 0.18 0.19 0.24 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 17 1 1 6 2 18 12 18 22 9 17 16 11 28 17 13 20 3 6 5 1 1 6
Bud 8 2 17 5 16 23 63 57 68 64 72 63 3 7 4 2 3 4 2 1 1 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 2 1 2 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 2 7 9 5 6 8 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 2 0 0 2 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 14 8 8 11 19 16 13 25 14 19 15 24 19 32 50 24 16 22 1 1 0 4 5 7
Endoplasmic Reticulum 4 15 0 1 0 1 2 4 1 8 3 13 3 3 8 4 8 3 0 0 0 1 0 1
Endosome 352 127 326 116 201 169 150 197 194 173 117 120 287 433 384 74 94 51 445 206 357 328 405 422
Golgi 6 15 4 2 3 0 0 2 4 7 1 3 3 9 6 25 24 27 12 10 13 7 14 27
Mitochondria 83 36 21 70 72 147 177 138 188 268 303 266 4 0 0 5 15 14 3 5 2 8 5 3
Nucleus 1 0 0 0 0 0 1 1 0 1 0 0 0 0 1 2 0 1 1 0 0 0 0 0
Nuclear Periphery 8 3 2 5 4 1 3 0 3 2 3 3 4 1 2 1 0 0 0 1 1 1 1 4
Nucleolus 66 18 38 32 47 35 53 43 37 39 41 34 11 11 11 4 7 9 1 0 0 2 3 3
Peroxisomes 6 0 97 83 77 77 101 97 67 98 63 66 30 14 13 0 3 0 9 4 28 4 12 6
SpindlePole 52 15 132 61 88 61 113 108 117 105 84 74 52 112 80 18 24 14 21 6 32 12 7 3
Vac/Vac Membrane 70 47 14 2 15 8 7 7 13 19 12 19 39 90 90 28 41 15 3 5 2 12 10 10
Unique Cell Count 460 220 429 259 348 391 478 492 502 610 530 528 341 527 510 145 171 130 508 255 449 387 474 504
Labelled Cell Count 679 303 660 389 550 544 708 700 733 833 733 710 471 724 677 204 248 181 508 255 449 387 474 504


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 9.0 7.9 7.7 7.9 6.9 6.7 6.8 6.8 6.8 6.3 6.6 9.7 10.0 9.6 13.8 13.5 12.7 9.4 10.0 11.1
Std Deviation (1e-4) 1.1 1.5 1.2 1.6 1.8 1.2 1.1 1.3 1.2 1.3 1.1 1.3 1.4 1.3 1.2 3.4 2.6 3.4 1.6 1.5 1.9
Intensity Change (Log2) – – – -0.03 -0.01 -0.19 -0.23 -0.22 -0.23 -0.21 -0.32 -0.26 0.29 0.34 0.27 0.8 0.78 0.69 0.26 0.34 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.7 2.8 3.6 3.6 0 3.4 4.2 2.6 4.6 0 0 0
Bud -1.5 0.4 1.3 4.9 4.2 5.1 3.9 5.1 4.4 -2.7 -2.6 -3.3 0 -1.4 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 2.7 1.9 0.9 2.6 0.9 1.2 1.0 2.3 2.8 3.2 5.0 6.7 4.2 6.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 3.3 0 0 0 0 0 0
Endosome -8.3 -5.4 -9.6 -13.4 -11.0 -11.4 -15.1 -16.7 -16.4 2.8 2.3 -0.2 -5.7 -5.1 -7.8
Golgi 0 0 0 0 0 0 0 0 0 0 1.0 0 7.8 6.9 8.7
Mitochondria 8.3 6.7 11.6 11.7 9.3 11.9 13.8 17.0 15.3 -2.9 -5.1 -5.1 -0.7 1.8 2.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.5 2.1 0 1.1 -0.1 -0.8 -1.5 -0.6 -1.4 -3.2 -4.7 -4.6 -2.4 -2.0 -0.7
Peroxisomes 2.7 -0.2 -1.0 -0.5 -1.1 -3.7 -2.7 -4.4 -4.1 -5.1 -9.6 -9.5 -6.3 -6.2 -6.0
SpindlePole -2.0 -1.7 -5.1 -2.4 -3.0 -2.6 -5.1 -5.5 -6.3 -5.0 -3.4 -5.5 -4.3 -4.2 -4.5
Vacuole -2.1 0.8 -1.1 -1.8 -1.9 -0.6 -0.1 -0.9 0.3 4.5 6.8 7.0 6.4 7.9 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.579 6.5784 6.4226 5.8247 5.5563 6.5745 5.5385 6.3925 6.0945 5.7465 5.5333 6.288 5.2319 6.5389 5.8215 5.8429 5.5509 5.7975
Actin 0.0069 0.0003 0.0009 0 0.0008 0.0005 0.0123 0.001 0.0066 0 0.0008 0.0006 0.0127 0.0005 0.0009 0.0014 0 0.0002
Bud 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0002 0.0005 0 0 0 0.0001 0 0 0.0003 0 0.0001
Bud Neck 0.0002 0.0001 0.0003 0.0001 0.0002 0.0003 0.0003 0.0005 0.0005 0.0001 0.0001 0.0012 0.0004 0.0001 0.0002 0.0003 0.0001 0.0002
Bud Periphery 0 0 0.0001 0 0 0 0.0001 0.0001 0.0006 0 0.0001 0 0.0001 0 0 0.0003 0 0
Bud Site 0.0003 0.0002 0.0004 0.0001 0.0002 0.0002 0.0014 0.0016 0.0043 0.0001 0.0001 0.0001 0.0006 0.0001 0.0001 0.0027 0.0001 0.0001
Cell Periphery 0 0 0 0 0 0 0.0001 0.0001 0.0003 0 0 0 0.0001 0 0 0.0001 0 0
Cytoplasm 0.0073 0.0096 0.0082 0.0017 0.0023 0.0078 0.0059 0.0111 0.0055 0.0019 0.004 0.0017 0.0037 0.0048 0.0027 0.004 0.002 0.0031
Cytoplasmic Foci 0.2321 0.2351 0.2392 0.1906 0.2539 0.2347 0.2062 0.2651 0.222 0.2305 0.2052 0.2194 0.212 0.249 0.2491 0.1196 0.2721 0.2155
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0009 0.0007 0.0008 0.0002 0.0001 0.0005 0.0027 0.0009 0.0007 0.0001 0.0028 0.0003 0.0005 0.0003 0.0002 0.0009 0.0004 0.0003
Endosome 0.5293 0.5662 0.5422 0.6671 0.5559 0.4748 0.588 0.5699 0.5437 0.6611 0.5861 0.5179 0.5689 0.566 0.5541 0.7191 0.5767 0.5418
Golgi 0.0823 0.0874 0.1312 0.1245 0.114 0.1748 0.0962 0.092 0.1233 0.0734 0.1041 0.1758 0.1069 0.1155 0.138 0.1122 0.0986 0.174
Lipid Particles 0.0619 0.0789 0.0289 0.0069 0.0234 0.0451 0.0493 0.0176 0.0379 0.0175 0.0353 0.0377 0.0515 0.0397 0.0235 0.0181 0.0215 0.032
Mitochondria 0.0013 0.0006 0.0012 0.0004 0.0005 0.0025 0.0039 0.0007 0.0172 0.0003 0.002 0.0028 0.0017 0.0003 0.0006 0.0019 0.0002 0.0009
None 0.0031 0.0001 0.0004 0 0 0.0001 0.0015 0.0011 0.0003 0 0.0001 0 0.0041 0 0 0.0006 0.0001 0
Nuclear Periphery 0.0023 0.0004 0.0003 0.0001 0.0001 0.0002 0.0062 0.0005 0.0004 0.0001 0.0154 0.0006 0.0006 0.0004 0.0001 0.0017 0.004 0.0002
Nucleolus 0.0001 0 0.0001 0 0 0 0.0002 0.0001 0.0004 0 0.0001 0.0003 0.0003 0 0 0.0004 0 0
Nucleus 0.0001 0.0001 0.0001 0 0 0.0001 0.0017 0.0002 0.0004 0 0.0004 0.0001 0.0004 0 0 0.0015 0.0001 0
Peroxisomes 0.0602 0.0142 0.0377 0.005 0.046 0.046 0.0146 0.0239 0.0253 0.0117 0.0223 0.0363 0.0297 0.0144 0.0247 0.0037 0.0193 0.025
Punctate Nuclear 0.0062 0.0016 0.0007 0.0001 0.0003 0.0005 0.0029 0.0007 0.0004 0.0003 0.0011 0.0006 0.0012 0.0003 0.0003 0.0027 0.001 0.0002
Vacuole 0.0035 0.0032 0.0052 0.0018 0.001 0.0093 0.0039 0.0107 0.007 0.0018 0.0093 0.0026 0.0028 0.0057 0.0037 0.0057 0.0021 0.0042
Vacuole Periphery 0.0017 0.0013 0.0021 0.0012 0.0012 0.0024 0.0024 0.0022 0.0026 0.0011 0.0107 0.0015 0.0013 0.0028 0.0017 0.0028 0.0017 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.8623 24.6775 12.3874 22.6492 14.7377 25.9672 27.8869 23.0319 24.8206 24.8636
Translational Efficiency 1.732 1.4892 2.3913 1.3949 2.0602 1.5916 1.3381 1.7326 1.4695 1.3893

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the endosomal Vps27p-Hse1p complex; complex is required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
Localization
Cell Percentages endosome (88%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Hse1

Hse1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hse1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available