Standard name
Human Ortholog
Description Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0.07 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0.05 0 0 0 0.09 0.08 0.1 – 0.17 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0.07 0.12 0.06 0.11 0.1 0.1 0.14 0.05 0 0 0.05 0.09 0 0.17 – 0.17 0.25 0.06 0.09 0.06 0.09 0.07 0.06
Golgi 0 0 0 0.16 0.17 0 0 0 0 0 0 0 0.18 0.23 0.25 – 0.17 0.07 0 0.06 0 0 0.09 0
Mitochondria 0.94 0.81 0.68 0.78 0.67 0.77 0.77 0.76 0.86 0.87 0.89 0.86 0.65 0.67 0.43 – 0 0.09 0.79 0.64 0.72 0.62 0.64 0.72
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0.05 0.06 0 0.05 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Peroxisomes 0 0.05 0.07 0 0.07 0 0 0.05 0 0.05 0 0 0 0 0.15 – 0.14 0.22 0.07 0 0.09 0.06 0.07 0
SpindlePole 0 0 0.1 0.06 0.07 0.05 0.07 0.09 0 0 0 0 0 0.06 0.11 – 0.17 0.18 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.25 0.23 0 0.11 0.1 0.07 0.06 0.07 0.08 0.06 0.05 0.1 0.08 0.1 – 0.17 0.21 0 0.09 0.05 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 6 13 2 0 1 3 0 1 0 0 3 6 3 – 0 2 0 3 3 0 3 1
Bud 3 1 0 7 3 4 9 12 7 12 7 7 2 3 1 – 1 2 0 1 1 0 0 2
Bud Neck 0 0 0 0 0 1 0 1 1 0 0 1 0 0 0 – 0 0 0 0 0 0 0 2
Bud Site 0 0 1 0 0 0 2 6 1 1 3 2 0 0 0 – 0 1 – – – – – –
Cell Periphery 3 5 4 13 4 3 2 3 4 2 3 1 4 4 6 – 1 4 0 0 0 0 0 0
Cytoplasm 4 5 3 7 9 9 11 13 13 8 8 7 10 7 14 – 6 8 0 0 0 1 1 1
Endoplasmic Reticulum 3 1 0 2 1 3 3 1 3 1 3 4 1 3 3 – 0 3 0 0 0 0 0 2
Endosome 7 13 12 14 21 30 37 63 15 11 0 18 10 3 23 – 6 23 12 19 9 4 15 12
Golgi 2 7 2 36 32 4 3 3 0 2 0 5 20 20 33 – 6 6 6 13 1 1 19 8
Mitochondria 188 156 66 174 128 235 277 334 245 297 204 311 74 59 58 – 1 8 162 142 101 33 137 137
Nucleus 0 0 0 1 3 7 10 5 2 9 6 7 3 1 2 – 1 4 0 1 0 0 1 1
Nuclear Periphery 10 3 0 2 1 16 20 10 13 8 9 18 0 1 0 – 0 1 2 1 0 0 1 0
Nucleolus 7 3 0 2 2 6 10 18 8 11 8 6 2 1 0 – 2 1 0 1 1 0 1 1
Peroxisomes 7 9 7 7 13 11 10 23 5 16 1 3 5 2 20 – 5 20 14 9 12 3 14 6
SpindlePole 6 6 10 14 13 16 25 38 12 13 6 15 4 5 15 – 6 16 0 4 1 1 2 3
Vac/Vac Membrane 16 48 22 5 21 32 24 26 19 28 13 18 11 7 13 – 6 19 2 19 6 1 10 6
Unique Cell Count 199 192 97 222 192 306 361 440 284 343 228 360 113 88 134 36 91 206 222 141 54 215 190
Labelled Cell Count 257 259 133 297 253 377 444 559 348 420 271 423 149 122 191 41 118 206 222 141 54 215 190


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.7 6.3 6.5 7.1 5.9 5.7 6.0 5.2 5.3 5.0 5.1 6.4 6.7 6.7 – 8.1 7.6 5.8 6.8 7.0
Std Deviation (1e-4) 0.8 1.2 1.4 1.2 1.4 1.4 1.1 1.4 1.1 1.2 1.4 0.9 0.9 1.0 1.3 – 2.0 1.9 1.2 1.2 1.1
Intensity Change (Log2) – – – 0.04 0.16 -0.11 -0.16 -0.07 -0.3 -0.27 -0.35 -0.31 0.01 0.07 0.09 – 0.35 0.25 -0.12 0.11 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 0 0 0 0 0 0 0 0 0 0.2 0 – 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0.6 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 1.7 0 2.1 – 0 1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Endosome -1.8 -0.4 -0.7 -0.6 0.5 -2.3 -3.6 0 -2.6 -0.8 -2.2 1.0 – 0 2.3
Golgi 3.6 3.6 0 0 0 0 0 0 0 3.7 4.3 4.7 – 0 0
Mitochondria 2.0 -0.2 1.7 1.8 1.6 4.0 4.2 4.7 4.2 -0.4 -0.1 -3.7 – -6.7 -8.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 -0.1 0 0 -0.8 0 -1.0 0 0 -0.9 0 1.8 – 0 2.9
SpindlePole -1.2 -1.0 -1.8 -1.1 -0.5 -2.2 -2.5 0 -2.4 -2.0 -1.2 0.2 – 0 1.4
Vacuole -6.0 -2.6 -3.1 -4.7 -5.2 -4.4 -4.0 -4.5 -5.5 -2.6 -2.8 -2.7 – -0.8 -0.3