Standard name
Human Ortholog
Description Lysine methyltransferase; involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.06 0.06 0 0 0 0 0 0
Cytoplasm 0.95 0.98 – 0.95 0.93 0.87 0.83 0.79 0.62 0.7 0.58 0.6 – 0.99 0.97 0.91 0.92 0.92 0.89 0.95 0.63 0.87 0.91 0.9
Endoplasmic Reticulum 0 0 – 0 0 0.05 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0.06 0.06 0.06 0.24 0.25 0.43 0.36 – 0 0 0 0 0 0 0 0.1 0 0 0
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0.05 0.1 0.11 0.19 0.13 0.07 0.1 – 0 0 0.07 0.06 0.08 0 0 0.08 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 3 1 1 3 1 0 1 3 – 0 0 2 0 2 2 0 0 0 0 0
Bud 0 0 – 0 0 0 2 0 3 2 3 2 – 0 0 0 0 0 1 0 0 0 1 0
Bud Neck 0 0 – 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 – 4 7 4 5 7 3 4 1 7 – 2 3 17 20 20 0 0 0 0 0 0
Cytoplasm 161 233 – 103 246 211 237 271 146 167 113 166 – 67 139 310 330 316 155 226 32 20 61 84
Endoplasmic Reticulum 2 3 – 0 3 11 12 17 3 0 6 4 – 0 1 10 14 13 1 1 2 0 0 1
Endosome 1 0 – 1 2 1 4 6 4 5 0 2 – 0 0 1 1 3 1 1 0 0 0 1
Golgi 0 0 – 1 0 0 0 1 0 0 0 0 – 0 0 1 0 2 0 0 0 0 0 0
Mitochondria 3 0 – 0 2 14 18 21 57 60 85 100 – 0 0 0 3 3 3 0 5 0 0 0
Nucleus 2 0 – 1 2 4 2 6 5 5 3 5 – 1 3 3 2 2 1 2 2 0 0 1
Nuclear Periphery 0 0 – 0 1 0 0 0 0 0 2 0 – 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 1 0 – 0 0 0 0 0 0 1 1 1 – 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 2 – 3 8 11 29 36 44 31 14 28 – 0 1 25 23 27 1 3 4 0 0 0
Unique Cell Count 169 237 108 264 243 285 341 237 240 196 276 68 143 340 359 344 174 239 52 23 67 93
Labelled Cell Count 171 241 113 274 257 311 369 266 275 229 318 70 147 369 394 389 174 239 52 23 67 93


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 10.7 – 9.4 9.3 7.5 7.3 7.4 6.1 6.0 5.5 5.5 – 8.7 8.5 12.6 12.9 11.8 – 8.1 10.0
Std Deviation (1e-4) 1.2 1.6 – 1.6 1.5 1.3 1.7 1.6 1.3 1.0 1.1 1.0 – 1.7 1.3 2.4 2.4 2.3 – 1.4 1.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7275 3.6258 3.3064 2.812 2.7118 3.022 2.5843 4.4851 3.685 3.1365 2.7241 3.9638 1.6508 2.7723 2.4848 2.1489 1.8355 2.2828
Actin 0.0139 0.0077 0.0039 0.0085 0.0114 0.0063 0.0095 0.0033 0.0023 0.0204 0.0336 0.0024 0.0363 0.0004 0.0033 0.0538 0.0115 0.0021
Bud 0.0074 0.0151 0.001 0.0176 0.0003 0.0041 0.0002 0.0003 0.0004 0.0025 0.0022 0.0002 0.0005 0.0003 0.0029 0.0008 0.0015 0.0002
Bud Neck 0.0015 0.0005 0.001 0.0005 0.0014 0.002 0.0015 0.0004 0.0003 0.0018 0.0011 0.0012 0.0026 0.0001 0.0008 0.007 0.0011 0.0006
Bud Periphery 0.0077 0.0047 0.001 0.0116 0.0002 0.0021 0.0002 0.0001 0.0005 0.001 0.0037 0.0001 0.0004 0.0001 0.0013 0.0008 0.0021 0.0002
Bud Site 0.0073 0.0172 0.014 0.0052 0.0005 0.0012 0.0038 0.0064 0.0005 0.0008 0.0034 0.0001 0.0024 0.0004 0.0021 0.0061 0.0038 0.0003
Cell Periphery 0.0006 0.0006 0.0004 0.0006 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0
Cytoplasm 0.6762 0.8538 0.8696 0.7818 0.7253 0.8679 0.8396 0.9305 0.9135 0.8689 0.5766 0.8817 0.7931 0.9396 0.9008 0.8472 0.8459 0.9059
Cytoplasmic Foci 0.0282 0.0155 0.0235 0.0141 0.032 0.0249 0.0269 0.0095 0.0203 0.0396 0.0606 0.0237 0.0282 0.0076 0.0137 0.0225 0.0188 0.0174
Eisosomes 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0002 0 0.0004 0 0.0001 0.0003 0.0001 0
Endoplasmic Reticulum 0.0128 0.0041 0.0048 0.0103 0.011 0.0063 0.0075 0.0016 0.0038 0.0036 0.0014 0.0045 0.0076 0.0017 0.0038 0.0055 0.0053 0.0026
Endosome 0.0767 0.0125 0.0158 0.016 0.087 0.0223 0.0382 0.0031 0.0319 0.0162 0.0887 0.0314 0.025 0.006 0.0143 0.0221 0.0334 0.0183
Golgi 0.0132 0.0021 0.0015 0.002 0.0063 0.0031 0.0046 0.0008 0.0037 0.0101 0.0371 0.0177 0.0036 0.0008 0.0014 0.0054 0.0052 0.0028
Lipid Particles 0.0144 0.0011 0.001 0.0003 0.0072 0.0015 0.0079 0.0001 0.0017 0.0001 0.0169 0.0038 0.0086 0 0.0005 0.0025 0.0041 0.0075
Mitochondria 0.0073 0.0025 0.0061 0.0263 0.0023 0.0015 0.0011 0.0004 0.0014 0.0056 0.0476 0.0196 0.0021 0.0007 0.0078 0.0014 0.0023 0.0012
None 0.0856 0.0529 0.0446 0.0708 0.0813 0.0469 0.0343 0.0409 0.0116 0.0048 0.0012 0.0054 0.0549 0.0392 0.0362 0.0113 0.0343 0.0151
Nuclear Periphery 0.0147 0.0013 0.001 0.0088 0.0129 0.0021 0.0019 0.0003 0.0008 0.0012 0.0012 0.0004 0.0104 0.0004 0.0013 0.0028 0.0062 0.0058
Nucleolus 0.0008 0.0003 0.0002 0.001 0.0005 0.0001 0.0006 0 0 0 0.0003 0 0.0009 0 0.0009 0.0002 0.0013 0.0006
Nucleus 0.006 0.0027 0.0018 0.0139 0.0052 0.003 0.0016 0.0007 0.0008 0.0018 0.0013 0.0008 0.0045 0.0007 0.0036 0.002 0.0057 0.0024
Peroxisomes 0.0042 0.0005 0.0016 0.0014 0.0015 0.0005 0.0043 0.0003 0.0033 0.0189 0.0893 0.0045 0.0059 0.0001 0.0006 0.0021 0.0017 0.0119
Punctate Nuclear 0.0058 0.0017 0.0026 0.0042 0.007 0.0011 0.0083 0.0001 0.0008 0.0003 0.0008 0.0002 0.0085 0.0002 0.0021 0.0027 0.0115 0.0028
Vacuole 0.0105 0.0028 0.0039 0.0035 0.0045 0.0024 0.0068 0.0008 0.0016 0.0019 0.0234 0.0014 0.0032 0.0014 0.0018 0.0027 0.0031 0.0014
Vacuole Periphery 0.0048 0.0004 0.0007 0.0015 0.0021 0.0007 0.0008 0.0001 0.0006 0.0005 0.0091 0.0008 0.0008 0.0002 0.0004 0.0007 0.0009 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.0362 25.8096 29.9768 32.9873 32.9578 37.0107 22.6922 20.6762 22.585 32.4679
Translational Efficiency 0.7992 1.0062 0.9301 0.7172 0.7903 0.8103 0.8776 0.9226 0.7778 0.7752

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1854 945 1435 1583 1018 1644 1092 2486 2872 2589 2527 4069

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 798.36 920.79 1147.92 1157.79 911.12 989.68 1151.11 1199.97 838.33 964.53 1149.30 1183.56
Standard Deviation 106.62 170.81 161.19 156.74 128.32 151.37 138.82 156.56 126.82 162.17 151.93 157.97
Intensity Change Log 2 — 0.205833 0.523911 0.536262 — 0.119321 0.337313 0.397285 — 0.160370 0.427468 0.463859

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000312 0.001057 0.000410 0.000563 0.000544 0.000469 0.000372 0.001099 0.000394 0.000683 0.000394 0.000890
Bud Neck 0.019092 0.007757 0.001796 0.002494 0.005632 0.005701 0.001958 0.003096 0.014321 0.006451 0.001866 0.002862
Bud Site 0.039261 0.075302 0.010491 0.032306 0.032359 0.041759 0.007179 0.019415 0.036815 0.054002 0.009060 0.024430
Cell Periphery 0.000109 0.000095 0.000082 0.000097 0.000143 0.000072 0.000078 0.000038 0.000121 0.000081 0.000080 0.000061
Cytoplasm 0.457702* 0.551504* 0.808662* 0.784762* 0.489038* 0.517137* 0.694298* 0.782414* 0.468810* 0.529682* 0.759241* 0.783327*
Cytoplasmic Foci 0.275835* 0.197190* 0.024106 0.047593 0.326372* 0.235642* 0.052599 0.031270 0.293748* 0.221606* 0.036419 0.037620
Eisosomes 0.000054 0.000028 0.000007 0.000007 0.000049 0.000023 0.000017 0.000008 0.000052 0.000025 0.000011 0.000008
Endoplasmic Reticulum 0.002715 0.001132 0.001275 0.000267 0.003027 0.001508 0.001696 0.000298 0.002826 0.001371 0.001457 0.000286
Endosome 0.037067 0.031186 0.002782 0.004503 0.031230 0.033244 0.008660 0.004739 0.034998 0.032493 0.005322 0.004647
Golgi 0.019155 0.010898 0.000636 0.005782 0.018104 0.016474 0.001465 0.004335 0.018782 0.014439 0.000994 0.004898
Lipid Particles 0.003512 0.001656 0.000747 0.000848 0.003126 0.001388 0.001552 0.000484 0.003375 0.001486 0.001095 0.000626
Mitochondria 0.017184 0.004874 0.000190 0.001301 0.002707 0.007080 0.000181 0.004263 0.012052 0.006275 0.000186 0.003111
Mitotic Spindle 0.004786 0.006038 0.007699 0.020513 0.002547 0.015111 0.006815 0.037738 0.003993 0.011799 0.007317 0.031037
None 0.002733 0.000660 0.002596 0.002094 0.000749 0.001003 0.002287 0.001330 0.002030 0.000878 0.002463 0.001627
Nuclear Periphery 0.000444 0.000134 0.000315 0.000131 0.000112 0.000116 0.000538 0.000166 0.000327 0.000123 0.000411 0.000152
Nuclear Periphery Foci 0.000503 0.001325 0.003345 0.001169 0.001198 0.000506 0.004300 0.000497 0.000750 0.000805 0.003758 0.000759
Nucleolus 0.000932 0.000396 0.000171 0.000192 0.000301 0.000468 0.000399 0.000290 0.000708 0.000441 0.000269 0.000252
Nucleus 0.022821 0.009819 0.100882 0.022481 0.015638 0.007273 0.057614 0.029992 0.020275 0.008202 0.082185 0.027070
Peroxisomes 0.005321 0.007629 0.000435 0.001260 0.003739 0.005216 0.000579 0.002342 0.004760 0.006097 0.000497 0.001921
Vacuole 0.078729 0.083216 0.032959 0.068509 0.056237 0.100529 0.155660 0.072404 0.070757 0.094210 0.085982 0.070889
Vacuole Periphery 0.011732 0.008105 0.000412 0.003127 0.007149 0.009280 0.001754 0.003781 0.010108 0.008851 0.000992 0.003527

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.98 -4.62 -2.57 4.52 0.96 1.12 1.63 -0.98 -1.46 -1.74 -5.08 -2.03 -2.18 0.77 -1.29
Bud Neck 8.38 13.57 13.42 7.60 -0.70 -0.25 6.80 6.09 6.54 -1.16 8.89 14.57 14.27 9.86 -1.32
Bud Site -7.74 11.98 6.39 11.57 -4.89 -3.03 11.23 8.36 12.84 -5.67 -6.68 16.42 11.89 16.78 -5.97
Cell Periphery 1.87 1.16 1.69 0.53 0.95 6.42 7.17 12.84 5.33 9.94 5.97 5.36 10.45 3.97 6.32
Cytoplasm -7.68 -36.26 -30.08 -15.99 4.95 -2.41 -13.86 -24.20 -24.39 -8.03 -7.41 -34.94 -39.98 -29.10 -1.75
Cytoplasmic Foci 9.83 47.42 43.48 23.62 -5.99 10.44 36.41 40.74 37.40 8.75 12.70 58.70 60.43 43.95 2.44
Eisosomes 7.53 15.41 15.86 10.99 1.17 9.21 10.67 15.22 11.98 8.62 12.02 18.19 21.02 16.26 7.29
Endoplasmic Reticulum 2.96 2.35 4.75 6.84 7.83 2.25 2.77 5.46 2.63 10.87 3.04 3.35 6.75 3.68 12.23
Endosome 2.42 21.33 21.61 12.32 0.10 -0.65 11.33 13.87 19.95 6.97 1.73 23.57 25.65 23.17 5.11
Golgi 6.15 18.39 12.74 6.02 -3.07 1.37 14.06 13.25 12.14 -1.50 4.48 22.77 18.79 13.40 -3.45
Lipid Particles 4.65 9.89 9.74 2.21 -0.13 5.22 5.04 8.72 4.24 9.58 7.67 10.76 13.56 4.49 7.19
Mitochondria 6.70 11.62 11.09 3.35 -2.02 -3.23 8.43 0.74 3.33 -3.09 4.50 12.39 10.02 4.51 -3.48
Mitotic Spindle -0.72 -2.37 -4.41 -3.50 -2.58 -5.36 -3.38 -8.05 -2.44 -5.73 -5.03 -3.84 -9.05 -3.67 -6.12
None 3.93 0.49 1.50 -1.54 1.52 -0.35 -1.79 -5.48 -0.68 0.84 2.30 -0.46 1.41 -1.34 2.11
Nuclear Periphery 1.15 0.13 0.80 -2.51 5.33 -0.02 -11.05 -5.04 -6.11 8.53 1.18 -1.13 0.40 -6.25 9.65
Nuclear Periphery Foci -4.27 -10.87 -7.46 0.55 8.21 4.88 -6.21 5.18 -0.87 7.38 -0.86 -11.60 -1.72 -0.25 11.29
Nucleolus 5.79 9.91 9.40 2.70 -2.20 -1.12 -1.32 -0.30 1.04 1.13 2.45 8.08 8.21 1.62 -0.48
Nucleus 8.95 -24.76 -7.36 -16.99 20.57 7.67 -17.53 -19.07 -30.72 6.39 12.59 -29.22 -17.30 -34.67 19.99
Peroxisomes -3.25 14.87 13.84 10.68 -3.08 -2.78 8.07 5.21 8.54 -3.43 -3.22 16.87 12.63 13.45 -4.32
Vacuole -1.81 7.77 -9.96 -7.05 -15.73 -11.21 -17.37 -17.73 -5.76 6.54 -8.27 -8.77 -18.05 -8.76 -5.58
Vacuole Periphery 3.63 17.02 12.35 6.35 -2.76 -1.74 6.85 5.99 8.12 -1.16 1.93 17.45 13.92 10.27 -3.06
Standard name
Human Ortholog
Description Lysine methyltransferase; involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Efm1

Efm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Efm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available