Standard name
Human Ortholog
Description tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD1 has a paralog, TCD2, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.12 0.09 0.12 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.1 0.1 0.06 0.06 0.08 0.07 0.08 0.06 0.07 0.16 0.18 0.23 0.21 0.11 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0 0.08 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.25 0.32 0.33 0.46 0.47 0.4 0 0.05 0 0 0.06 0.06
Mitochondria 0.9 0.91 0.83 0.85 0.83 0.88 0.92 0.86 0.87 0.92 0.94 0.92 0.58 0.35 0.45 0.09 0.08 0.08 0.78 0.75 0.79 0.69 0.66 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0.05 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.15 0.11 0.07 0.12 0.07 0.05 0.06 0.07 0.05 0.07 0.06 0.21 0.34 0.18 0.19 0.31 0.27 0 0.07 0 0.07 0.1 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 1 1 0 0 0 0 0 0 0 8 2 2 6 5 3 1 3 7 3 5 12
Bud 0 0 7 11 4 14 24 16 22 29 21 23 1 1 0 1 6 5 6 3 4 4 17 13
Bud Neck 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 1 3 3
Bud Site 3 2 1 0 1 4 9 5 2 11 9 19 0 0 0 0 1 0 – – – – – –
Cell Periphery 7 2 12 11 6 4 4 0 4 7 6 6 12 20 9 23 16 18 0 0 1 0 0 1
Cytoplasm 10 7 24 35 39 32 47 60 43 57 40 48 52 80 78 39 20 16 1 0 0 13 12 13
Endoplasmic Reticulum 0 2 0 1 2 4 1 0 1 3 3 1 0 4 2 7 7 3 1 1 0 1 5 2
Endosome 11 4 36 13 13 4 2 15 1 1 1 2 12 38 22 1 3 1 7 6 16 16 17 19
Golgi 0 6 9 3 0 0 0 0 0 0 0 0 81 142 114 88 84 62 7 9 15 14 30 34
Mitochondria 208 164 354 314 341 489 704 672 539 625 619 645 187 157 154 17 14 13 181 140 356 229 339 371
Nucleus 0 1 4 0 3 4 10 17 13 10 2 12 2 1 0 6 2 2 0 0 1 6 6 8
Nuclear Periphery 1 4 9 3 7 33 39 22 22 16 21 11 3 1 2 3 1 2 3 0 7 2 1 4
Nucleolus 11 4 9 7 14 27 45 41 23 21 22 16 4 1 2 1 3 3 1 0 1 0 3 3
Peroxisomes 8 3 33 18 9 3 0 9 5 0 4 3 6 7 22 3 2 7 6 2 24 4 3 9
SpindlePole 4 1 11 4 12 8 14 30 18 9 17 22 0 7 2 1 2 2 2 1 6 2 8 9
Vac/Vac Membrane 27 28 45 24 51 41 38 47 44 37 43 42 68 153 62 36 55 42 7 13 3 24 50 46
Unique Cell Count 232 181 428 368 409 553 766 784 622 678 656 704 320 444 343 190 180 155 233 186 453 333 513 563
Labelled Cell Count 290 228 556 445 504 667 938 934 737 826 808 850 436 614 471 232 221 179 233 186 453 333 513 563


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 8.2 7.1 6.5 6.1 4.9 4.6 4.6 4.3 4.0 3.7 3.6 8.5 8.4 7.8 11.1 10.7 10.4 8.7 9.1 9.5
Std Deviation (1e-4) 1.8 2.7 2.3 2.4 2.2 1.7 1.6 1.8 1.7 1.6 1.2 1.2 2.2 2.3 2.1 2.4 2.3 2.1 2.5 2.2 2.6
Intensity Change (Log2) – – – -0.13 -0.22 -0.53 -0.63 -0.64 -0.72 -0.82 -0.95 -0.97 0.26 0.25 0.14 0.64 0.58 0.54 0.28 0.36 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1.3 -0.8 1.0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.2 -1.3 -2.6 0 0 0 0 0 0 0.7 1.3 -0.2 4.6 3.3 4.3
Cytoplasm 2.1 2.2 0.1 0.4 1.3 0.9 1.7 0.3 0.8 4.8 5.7 7.0 5.7 2.4 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -2.9 -3.2 -6.0 0 0 0 0 0 0 -2.6 0.1 -1.0 -3.8 -3.1 -3.4
Golgi -1.5 0 0 0 0 0 0 0 0 9.7 11.7 11.7 13.9 13.9 12.4
Mitochondria 1.0 0.3 2.6 0 0 0 0 0 0 -7.3 -14.2 -11.0 -17.3 -17.3 -16.4
Nucleus 0 0 0 0.6 1.6 1.5 0.8 0 1.1 0 0 0 0 0 0
Nuclear Periphery -1.5 -0.4 3.0 0 0 0 0 0 0 -1.3 0 0 0 0 0
Nucleolus -0.2 1.2 2.3 0 0 0 0 0 0 -0.9 0 0 0 0 0
Peroxisomes -1.6 -3.6 -5.9 0 0 0 0 0 0 -3.6 -4.3 -0.7 -3.0 -3.2 -1.3
SpindlePole -1.5 0.3 -1.3 0 0 0 0 0 0 0 -1.0 -2.1 0 0 0
Vacuole -2.0 0.9 -1.7 -3.6 -2.8 -2.0 -3.1 -2.3 -2.8 4.1 8.4 3.0 2.9 6.1 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1855 2.5045 2.3628 2.9422 2.0491 2.7456 1.749 1.2206 1.2988 1.6564 1.0921 1.2733 0.1648 0.6569 0.6796 1.0189 0.4683 0.5512
Actin 0.0219 0.0093 0.0407 0.0197 0.0252 0.0194 0.1102 0.0238 0.0408 0.0713 0.062 0.0237 0.0272 0.0191 0.0052 0.0055 0.0062 0.0201
Bud 0.0013 0.0074 0.007 0.006 0.0045 0.0034 0.0025 0.0105 0.0087 0.0013 0.0079 0.0023 0.0031 0.0076 0.0021 0.0023 0.0049 0.001
Bud Neck 0.002 0.0082 0.0034 0.0007 0.0012 0.005 0.0023 0.0026 0.0025 0.0009 0.0021 0.0049 0.0076 0.0011 0.0014 0.0008 0.0045 0.0055
Bud Periphery 0.0037 0.0227 0.019 0.033 0.0428 0.0204 0.0047 0.014 0.0247 0.0032 0.0462 0.0111 0.0108 0.0203 0.0079 0.013 0.0174 0.004
Bud Site 0.0064 0.0841 0.0286 0.0045 0.0014 0.0018 0.0111 0.094 0.0251 0.0021 0.0034 0.0013 0.0135 0.0265 0.0121 0.0017 0.0026 0.0017
Cell Periphery 0.0017 0.0045 0.0071 0.0083 0.0027 0.003 0.0011 0.0034 0.0016 0.0009 0.0019 0.0012 0.001 0.0011 0.0021 0.0021 0.0011 0.0016
Cytoplasm 0.0295 0.0411 0.0337 0.0025 0.0305 0.0639 0.0329 0.0243 0.0343 0.0051 0.0399 0.0577 0.0212 0.0303 0.0347 0.026 0.016 0.032
Cytoplasmic Foci 0.0595 0.0562 0.0492 0.0537 0.0645 0.0484 0.0863 0.0431 0.0494 0.0372 0.0766 0.0495 0.0769 0.0757 0.0503 0.0308 0.0543 0.0641
Eisosomes 0.0016 0.0009 0.0015 0.0004 0.0004 0.0007 0.0016 0.0019 0.0007 0.0007 0.0009 0.0006 0.0007 0.0007 0.0006 0.0005 0.0002 0.0003
Endoplasmic Reticulum 0.0083 0.0027 0.0024 0.0006 0.0015 0.0021 0.0113 0.0041 0.0024 0.0012 0.0049 0.0047 0.0045 0.0014 0.0034 0.0088 0.0026 0.0013
Endosome 0.0785 0.1135 0.0586 0.0203 0.0495 0.0552 0.098 0.1053 0.0556 0.0572 0.1053 0.0734 0.1456 0.0968 0.0937 0.0617 0.0414 0.0628
Golgi 0.0415 0.0425 0.0324 0.02 0.033 0.0292 0.0412 0.0358 0.0298 0.0396 0.04 0.0552 0.0494 0.0448 0.0381 0.031 0.032 0.0429
Lipid Particles 0.0249 0.011 0.0116 0.0244 0.0062 0.013 0.043 0.0141 0.0101 0.0135 0.0134 0.0274 0.032 0.0074 0.011 0.0016 0.0055 0.0094
Mitochondria 0.4541 0.4364 0.5573 0.6545 0.6072 0.6155 0.3863 0.5063 0.5761 0.7221 0.4791 0.5504 0.4478 0.5138 0.6214 0.7272 0.5277 0.6563
None 0.1872 0.0704 0.1129 0.0649 0.091 0.0719 0.0783 0.0554 0.0968 0.0065 0.0207 0.0973 0.0833 0.0897 0.067 0.0606 0.1561 0.0587
Nuclear Periphery 0.0226 0.001 0.0006 0.0003 0.0004 0.0008 0.0133 0.0016 0.0015 0.0011 0.0074 0.0032 0.0108 0.0004 0.0013 0.0017 0.0217 0.0003
Nucleolus 0.0013 0.0042 0.0014 0.0041 0.0003 0.0006 0.0014 0.0025 0.0012 0.0005 0.0014 0.0004 0.0018 0.0016 0.0009 0.0004 0.0039 0.0003
Nucleus 0.0113 0.0016 0.0009 0.0013 0.0006 0.0006 0.0017 0.002 0.0029 0.0009 0.0019 0.0012 0.0021 0.0012 0.0011 0.0007 0.0053 0.0005
Peroxisomes 0.0256 0.0348 0.0159 0.056 0.0278 0.0307 0.0448 0.0236 0.0228 0.0251 0.049 0.0218 0.0344 0.0488 0.0273 0.0136 0.0644 0.0302
Punctate Nuclear 0.0043 0.001 0.0009 0.0006 0.0005 0.0015 0.0142 0.0016 0.0038 0.0011 0.0087 0.0037 0.003 0.0013 0.001 0.0003 0.0035 0.0005
Vacuole 0.0079 0.0353 0.0113 0.0184 0.0059 0.0087 0.0086 0.0227 0.006 0.0044 0.0196 0.0051 0.0138 0.0073 0.0115 0.0068 0.0216 0.0039
Vacuole Periphery 0.0049 0.011 0.0035 0.0058 0.0031 0.0043 0.0054 0.0074 0.0033 0.0044 0.0077 0.004 0.0096 0.003 0.0059 0.003 0.007 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.1008 29.8207 15.3195 23.9759 18.853 14.5329 20.4877 16.6977 21.1092 23.4195
Translational Efficiency 0.9625 0.6809 1.3696 0.8511 1.0208 1.2635 0.9838 1.0045 0.8284 0.7876

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD1 has a paralog, TCD2, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (89%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Tcd1

Tcd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tcd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available