Standard name
Human Ortholog
Description Serine/threonine MAP kinase; coordinates expression of all 19S regulatory particle assembly-chaperones (RACs) to control proteasome abundance; involved in regulating maintenance of cell wall integrity, cell cycle progression, nuclear mRNA retention in heat shock, septum assembly; required for mitophagy, pexophagy; affects recruitment of mitochondria to phagophore assembly site; plays role in adaptive response of cells to cold; regulated by the PKC1-mediated signaling pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.83 0.87 0.6 0.61 0.7 0.48 0.53 0.61 0.5 0.51 0.5 0.8 0.8 0.92 0.54 0.6 0.61 0.66 0.62 0.61 0.47 0.48 0.33
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0.08 0.15 0.23 0.22 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.05 0 0.14 0.12 0.06 0.09 0.11 0.11 0.11 0.11 0.1 0 0 0 0 0.05 0 0.2 0.21 0.19 0.28 0.22 0.25
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.15 0.21 0.37 0.41 0.28 0.44 0.31 0.18 0.25 0.21 0.21 0.27 0.34 0.13 0.52 0.45 0.52 0 0.11 0.07 0 0.06 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 2 1 1 1 0 0 0 0 0 0 1 0 1 2 1 0
Bud 0 1 0 0 0 0 2 1 0 3 1 3 1 0 0 1 0 0 7 1 6 5 9 23
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 1 2 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 3 3 1 2 2 4 0 0 0 2 2 0 0 0 0 0 0 0
Cytoplasm 339 100 169 68 138 183 196 224 136 149 108 159 128 211 283 87 82 79 300 81 137 42 82 76
Endoplasmic Reticulum 0 1 2 1 1 6 2 8 7 3 1 7 4 0 2 6 4 5 2 1 4 0 3 9
Endosome 0 0 1 1 0 0 2 3 0 0 1 0 0 2 1 1 2 1 13 1 4 3 5 7
Golgi 0 1 1 2 4 0 3 3 1 0 3 4 0 0 0 2 3 2 2 1 1 0 2 0
Mitochondria 6 1 2 0 3 7 23 36 33 68 47 80 0 2 1 4 1 1 1 0 0 1 0 0
Nucleus 10 6 6 16 27 16 37 47 24 32 24 31 4 3 5 2 7 4 91 27 42 25 38 58
Nuclear Periphery 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 2 0 2 1 1 2
Nucleolus 0 1 0 1 0 0 0 2 1 4 3 0 0 0 0 0 0 0 0 0 0 0 3 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 1 2 7 5
Vac/Vac Membrane 67 18 41 42 92 74 178 132 40 76 44 68 44 89 41 84 62 68 11 13 15 1 10 33
Unique Cell Count 385 120 194 114 226 260 406 424 224 300 213 321 161 264 307 162 137 130 455 131 227 90 174 230
Labelled Cell Count 422 129 222 131 266 286 447 459 246 340 235 357 181 307 333 189 163 160 455 131 227 90 174 230


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 10.5 9.7 9.9 11.7 11.3 10.7 11.2 11.2 10.1 10.5 11.0 10.3 10.4 10.4 13.2 14.6 17.5 9.2 12.3 16.2
Std Deviation (1e-4) 1.2 1.5 1.7 1.6 2.4 2.3 2.4 2.3 2.4 2.8 2.5 2.6 1.3 1.6 1.8 5.0 5.2 7.1 2.6 3.2 5.3
Intensity Change (Log2) – – – 0.02 0.27 0.22 0.13 0.2 0.2 0.05 0.11 0.17 0.08 0.1 0.09 0.44 0.59 0.85 -0.08 0.34 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -5.5 -6.0 -4.2 -9.1 -8.2 -6.1 -8.5 -7.9 -8.6 -1.9 -2.0 1.9 -7.0 -5.7 -5.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.7 3.6 5.0 6.7 6.5 7.2 0 0 0 0 0 0
Nucleus 3.6 3.4 1.5 2.7 3.3 3.0 3.1 3.2 2.8 0 0 0 0 0.9 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.0 4.3 1.8 5.4 2.6 -0.8 1.1 -0.1 0 1.4 3.0 -2.3 6.0 4.7 5.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9007 5.8455 4.6944 4.3198 3.9091 4.611 5.5099 6.203 6.3235 5.4453 5.9085 5.9139 7.666 8.5895 7.9324 7.9109 7.0657 8.1059
Actin 0.008 0.0002 0.0005 0.001 0.0003 0.0025 0.0489 0.0011 0.0034 0.0112 0.0117 0.001 0.0266 0.0014 0.0009 0.0007 0.0006 0.0041
Bud 0.0001 0.0001 0.0001 0.0006 0.0002 0.0002 0.0006 0.0004 0.0003 0.0013 0.0002 0.0002 0.0005 0.0006 0.0002 0.0002 0.0001 0.0002
Bud Neck 0.0008 0.0005 0.0007 0.0023 0.0021 0.0035 0.0086 0.0007 0.0009 0.0045 0.0017 0.0024 0.0029 0.0017 0.0009 0.0014 0.0016 0.0034
Bud Periphery 0.0001 0 0 0.0003 0.0001 0.0001 0.0007 0.0001 0.0001 0.0016 0.0001 0.0001 0.0006 0.0002 0 0 0 0.0001
Bud Site 0.0003 0.0006 0.0004 0.0015 0.0002 0.0002 0.0016 0.0017 0.0009 0.0173 0.001 0.0003 0.0007 0.0082 0.0005 0.0003 0.0001 0.0002
Cell Periphery 0 0 0 0 0 0 0.0018 0 0.0001 0.0004 0.0001 0 0.0001 0.0001 0 0 0 0
Cytoplasm 0.4747 0.4615 0.4416 0.4933 0.5296 0.3667 0.4659 0.6773 0.5253 0.6883 0.6695 0.5262 0.4864 0.5968 0.551 0.6111 0.6712 0.4642
Cytoplasmic Foci 0.005 0.001 0.0105 0.0028 0.0022 0.0053 0.017 0.0032 0.0093 0.0245 0.0151 0.0064 0.0056 0.0037 0.0022 0.0034 0.0026 0.0036
Eisosomes 0.0001 0 0 0 0 0 0.0003 0 0 0.0003 0.0001 0 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.0062 0.0019 0.0051 0.0057 0.0038 0.004 0.008 0.0037 0.0038 0.0028 0.0028 0.0047 0.0057 0.0038 0.0034 0.0025 0.0057 0.0047
Endosome 0.0068 0.0006 0.0064 0.0073 0.0022 0.0054 0.0187 0.0016 0.0058 0.0031 0.0065 0.0082 0.0066 0.0026 0.0015 0.0014 0.0025 0.004
Golgi 0.0014 0 0.0002 0.0002 0 0.0021 0.0091 0.0001 0.0025 0.0013 0.0079 0.0019 0.0023 0.0002 0.0001 0.0001 0.0001 0.0011
Lipid Particles 0.0014 0 0.0004 0.0001 0 0.0005 0.0081 0.0002 0.0051 0.0009 0.0126 0.0018 0.0073 0.0001 0 0 0 0.0006
Mitochondria 0.0006 0.0002 0.0003 0.0009 0.0003 0.0008 0.0078 0.0004 0.0019 0.0038 0.0018 0.0007 0.0024 0.0003 0.0004 0.0004 0.0002 0.0008
None 0.0282 0.0133 0.0208 0.0195 0.0134 0.0171 0.0157 0.0106 0.0167 0.0084 0.0125 0.0259 0.0042 0.0022 0.0027 0.0057 0.0025 0.0036
Nuclear Periphery 0.0246 0.0165 0.0223 0.0184 0.0176 0.0171 0.014 0.011 0.0111 0.0086 0.0074 0.0125 0.0292 0.0138 0.0148 0.0098 0.0144 0.0161
Nucleolus 0.0006 0.0005 0.0007 0.0011 0.0009 0.0013 0.0008 0.0003 0.0008 0.0007 0.0001 0.0003 0.001 0.0003 0.0004 0.0003 0.0002 0.0006
Nucleus 0.4303 0.4921 0.4801 0.4361 0.404 0.56 0.3452 0.2786 0.397 0.1892 0.2108 0.3972 0.4019 0.3548 0.4156 0.3547 0.2882 0.48
Peroxisomes 0.0006 0 0.0003 0.0002 0 0.0003 0.0112 0.0001 0.0093 0.0203 0.0321 0.0008 0.0047 0.0002 0.0001 0.0002 0.0001 0.0002
Punctate Nuclear 0.0072 0.0099 0.0055 0.0071 0.021 0.0102 0.0135 0.0079 0.0044 0.0096 0.0044 0.0081 0.0073 0.0075 0.0047 0.0068 0.0088 0.0108
Vacuole 0.0026 0.0008 0.0033 0.0016 0.0016 0.0023 0.0021 0.0009 0.0011 0.0016 0.0013 0.0011 0.0027 0.0011 0.0007 0.0007 0.0009 0.0012
Vacuole Periphery 0.0004 0.0001 0.0005 0.0003 0.0003 0.0004 0.0006 0.0001 0.0002 0.0004 0.0003 0.0003 0.0009 0.0002 0.0002 0.0002 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.5719 44.7428 31.1889 37.7448 59.9776 43.2022 43.3545 45.569 41.2946 53.5018
Translational Efficiency 0.8919 1.1348 0.8256 0.6526 0.581 0.8525 0.8125 0.5547 0.6594 0.7259

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Serine/threonine MAP kinase; coordinates expression of all 19S regulatory particle assembly-chaperones (RACs) to control proteasome abundance; involved in regulating maintenance of cell wall integrity, cell cycle progression, nuclear mRNA retention in heat shock, septum assembly; required for mitophagy, pexophagy; affects recruitment of mitochondria to phagophore assembly site; plays role in adaptive response of cells to cold; regulated by the PKC1-mediated signaling pathway
Localization
Cell Percentages cytoplasm (88%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Slt2

Slt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available