Standard name
Human Ortholog
Description Protein of unknown function; required for survival upon exposure to K1 killer toxin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 – 0.06 0 0 0 0 0 0.06
Bud 0 0 0.08 0 0 0 – 0 0 0.05 0 0 0 0 0 0.06 – 0.09 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0.06 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 – 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.08 0.05 0 – 0 0 0 0 0 0.05 0 0 0.19 – 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 – 0 0 0 0 0 0.05 0 0.09 0.06 – 0.11 0.07 0.07 0.05 0 0.05 0.07
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.27 – 0.16 0.05 0.09 0.05 0.07 0.11 0.1
Mitochondria 0.95 0.92 0.95 0.92 0.88 0.88 – 0.89 0.96 0.94 0.94 0.97 0.84 0.87 0.84 0 – 0.1 0.77 0.68 0.76 0.73 0.62 0.55
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0.05 0 0 0.07 – 0.07 0.09 0.06 0.05 0.08 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.09 – 0.07 0.11 0.07 0.07 0.05 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.07 0.09 0.13 – 0.12 0 0.06 0.06 0 0.05 0.05
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 – 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.19 0.07 0 0.07 0 – 0 0 0 0 0 0.13 0.09 0.13 0.1 – 0.1 0.05 0.05 0 0.06 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 2 0 1 0 – 0 0 0 0 0 0 2 0 5 – 6 3 1 0 2 9 21
Bud 5 1 5 4 7 2 – 8 5 17 8 5 5 5 11 5 – 9 0 0 0 0 1 7
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 1 1
Bud Site 1 0 0 1 0 1 – 5 1 8 3 4 1 0 0 3 – 6 – – – – – –
Cell Periphery 5 1 2 4 0 0 – 2 0 1 0 3 10 5 8 8 – 4 0 0 0 0 0 0
Cytoplasm 0 1 3 9 8 6 – 9 7 7 6 6 11 15 15 17 – 15 0 0 1 1 1 3
Endoplasmic Reticulum 6 1 1 0 1 1 – 1 1 1 1 3 1 5 1 1 – 8 0 0 0 0 5 0
Endosome 1 0 0 0 9 3 – 1 0 1 0 0 12 14 34 5 – 11 24 6 3 4 14 25
Golgi 5 3 0 0 3 1 – 0 0 0 0 0 6 7 16 24 – 17 18 9 3 8 30 34
Mitochondria 332 88 57 105 141 140 – 187 223 307 213 275 190 308 322 4 – 10 270 70 54 84 165 191
Nucleus 0 0 2 0 0 3 – 3 3 3 0 4 1 1 0 4 – 4 0 0 0 0 1 2
Nuclear Periphery 18 1 3 0 7 12 – 14 20 20 12 23 1 13 4 1 – 3 3 0 0 1 3 4
Nucleolus 7 3 0 4 7 15 – 15 25 23 16 13 3 5 2 0 – 3 0 0 0 0 2 3
Peroxisomes 0 2 0 1 6 5 – 5 1 7 1 1 19 25 34 12 – 12 7 6 4 2 14 16
SpindlePole 2 0 0 0 3 1 – 4 3 8 3 5 3 4 6 6 – 12 1 0 0 0 1 2
Vac/Vac Membrane 53 18 4 3 11 7 – 6 4 9 6 9 30 33 49 9 – 10 18 4 1 7 12 25
Unique Cell Count 349 96 60 114 160 160 209 232 325 227 284 226 354 384 89 104 351 104 71 117 268 347
Labelled Cell Count 437 120 79 131 204 197 260 293 412 269 351 293 442 502 105 130 351 104 71 117 268 347


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.7 4.3 5.2 6.0 5.3 – 5.1 4.4 4.3 4.6 4.6 6.7 6.7 6.6 7.2 – 7.6 6.5 7.3 7.4
Std Deviation (1e-4) 0.9 1.3 0.9 1.8 2.1 2.7 – 1.9 1.4 1.0 2.2 1.3 1.8 1.9 1.9 2.0 – 2.3 1.6 1.8 2.0
Intensity Change (Log2) – – – 0.28 0.5 0.31 – 0.27 0.04 0.01 0.12 0.1 0.66 0.65 0.63 0.76 – 0.83 0.62 0.78 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud 0 0 0 – 0 – – – – 0 0 0 0 – 0.1
Bud Neck 0 0 0 – 0 – – – – 0 0 0 0 – 0
Bud Site 0 0 0 – 0 – – – – 0 0 0 0 – 0
Cell Periphery 0 0 0 – 0 – – – – 0 0 0 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum 0 0 0 – 0 – – – – 0 0 0 0 – 0
Endosome 0 0 0 – 0 – – – – 0 0 0 0 – 0
Golgi 0 0 0 – 0 – – – – 0 0 0 4.4 – 3.3
Mitochondria -0.7 -1.5 -1.6 – -1.3 – – – – -2.2 -1.8 -2.3 -11.0 – -10.7
Nuclear Periphery 0 0 0 – 0 – – – – 0 0 0 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus 0 0 0 – 0 – – – – 0 0 0 0 – 0
Peroxisomes 0 0 0 – 0 – – – – 0 0 0 0 – 0
SpindlePole 0 0 0 – 0 – – – – 0 0 0 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches 0 0 0 – 0 – – – – 0 0 0 0 – 0
Cytoplasm 0 0 0 – 0 – – – – 0 0 0 2.5 – 1.9
Nucleus 0 0 0 – 0 – – – – 0 0 0 0 – 0
Vacuole 0 0 0 – 0 – – – – 1.4 0.7 1.4 0.7 – 0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1867 2.7503 2.911 2.4439 1.8745 2.767 2.0402 2.2295 2.2176 2.1462 1.8477 2.2974 4.4869 5.9924 5.9029 3.9165 3.5463 5.2446
Actin 0.015 0.0056 0.0121 0.0017 0.0191 0.0042 0.0127 0.0183 0.0138 0.0046 0.0299 0.0069 0.0618 0.0122 0.0277 0.0026 0.0575 0.0071
Bud 0.0002 0.0019 0.0009 0.0011 0.0003 0.0001 0.0007 0.0032 0.0007 0.0003 0.0005 0.0003 0.0023 0.0022 0.0004 0.0015 0.0009 0.0002
Bud Neck 0.0013 0.0005 0.0006 0.0001 0.0004 0.0001 0.0007 0.0004 0.0031 0.0117 0.0006 0.0005 0.0023 0.0002 0.0004 0.0004 0.0025 0.0002
Bud Periphery 0.0004 0.0019 0.0013 0.0025 0.0006 0.0005 0.0012 0.0036 0.0015 0.0004 0.001 0.0007 0.0025 0.0014 0.0005 0.009 0.002 0.0005
Bud Site 0.0012 0.0054 0.0086 0.0009 0.0009 0.0001 0.0013 0.0068 0.003 0.0012 0.0008 0.0004 0.0058 0.0059 0.0012 0.0012 0.0033 0.0002
Cell Periphery 0.0002 0.0003 0.0004 0.0001 0.0001 0.0001 0.0002 0.0004 0.0006 0.0001 0.0002 0.0002 0.0004 0.0006 0.0002 0.0025 0.0005 0.0001
Cytoplasm 0.0016 0.0008 0.0008 0.0001 0.0005 0.0004 0.0016 0.0009 0.0052 0.0002 0.0003 0.0005 0.0016 0.001 0.0011 0.0006 0.0007 0.0002
Cytoplasmic Foci 0.019 0.014 0.0204 0.0028 0.0152 0.0047 0.0142 0.0083 0.0156 0.0155 0.014 0.014 0.0103 0.0139 0.0183 0.0063 0.0258 0.0079
Eisosomes 0.0002 0.0001 0.0013 0.0001 0.0002 0.0001 0.0003 0.0002 0.0009 0.0001 0.0002 0.0002 0.0012 0.0002 0.0006 0.0002 0.0013 0.0002
Endoplasmic Reticulum 0.0008 0.0003 0.0005 0.0001 0.0005 0.0005 0.0007 0.0001 0.0014 0 0.0002 0.0002 0.0014 0.0001 0.0004 0.0001 0.0012 0.0003
Endosome 0.0431 0.0474 0.0231 0.0156 0.0163 0.0106 0.0494 0.0131 0.024 0.0148 0.046 0.0182 0.0248 0.0321 0.0145 0.0076 0.0406 0.0118
Golgi 0.0376 0.0644 0.0334 0.016 0.0177 0.0211 0.0664 0.0394 0.0395 0.033 0.0754 0.0364 0.0291 0.0422 0.0305 0.0134 0.0861 0.0227
Lipid Particles 0.031 0.0066 0.014 0.0017 0.0254 0.0169 0.033 0.0113 0.0092 0.0038 0.0064 0.0096 0.0346 0.0185 0.008 0.0068 0.0295 0.0116
Mitochondria 0.7957 0.8068 0.8437 0.9382 0.8434 0.9127 0.7695 0.8693 0.8402 0.8967 0.7706 0.8853 0.7363 0.7825 0.8285 0.8953 0.675 0.8834
None 0.001 0.0001 0.0004 0.0001 0.0005 0.0019 0.0078 0.0002 0.001 0 0.0002 0.0006 0.0059 0.0019 0.0141 0.0002 0.002 0.0002
Nuclear Periphery 0.0062 0.0001 0.0025 0.0001 0.0016 0.0003 0.0046 0 0.0048 0 0.0003 0.0002 0.0014 0.0001 0.0008 0 0.0026 0.0075
Nucleolus 0.0004 0.0008 0.0005 0.0001 0.0004 0.0001 0.0004 0.0002 0.0002 0.0002 0.0002 0.0002 0.0012 0.0101 0.0051 0.0003 0.001 0.0113
Nucleus 0.002 0.0005 0.0006 0.0001 0.0005 0.0001 0.0037 0.0001 0.0012 0.0001 0.0002 0.0001 0.0012 0.0085 0.0069 0.0002 0.0009 0.0025
Peroxisomes 0.0273 0.0187 0.0294 0.0107 0.0487 0.0223 0.0242 0.0215 0.0302 0.0156 0.0436 0.0211 0.0626 0.0573 0.0351 0.0464 0.0535 0.0274
Punctate Nuclear 0.0068 0.0001 0.0015 0 0.0056 0.0002 0.0002 0 0.0003 0.0001 0.0005 0.0024 0.0036 0.0001 0.0042 0 0.0076 0.0007
Vacuole 0.0042 0.0183 0.0017 0.0021 0.001 0.0009 0.0033 0.0016 0.002 0.0007 0.0044 0.0007 0.0066 0.0036 0.0006 0.0036 0.003 0.0009
Vacuole Periphery 0.0047 0.0053 0.0022 0.0059 0.0011 0.0018 0.0039 0.0011 0.0016 0.0008 0.0047 0.0013 0.003 0.0053 0.0009 0.0019 0.0024 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.8459 17.569 9.3121 19.2965 25.4816 8.4183 21.5706 15.4151 20.5634 23.2859
Translational Efficiency 3.0129 2.3501 3.9533 2.0628 1.7079 7.8486 4.3375 4.5369 3.2059 2.4909

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; required for survival upon exposure to K1 killer toxin
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Fyv4

Fyv4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fyv4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available