Standard name
Human Ortholog
Description Mitochondrial 3-hydroxyacyl-thioester dehydratase; involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0.05 0.06 0.06 0.05 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.64 0.88 0.89 0.87 0.84 0.83 0.77 0.79 0.52 0.47 0.34 0.46 0.97 0.95 0.92 – – – 0.35 0.6 0.41 0.68 0.7 0.62
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.36 0.19 0 0 0 0.1 0.16 0.13 0.5 0.55 0.69 0.57 0 0 0 – – – 0.42 0.19 0.31 0.15 0.12 0.15
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0.08 0 0.1 0.08 0.11 0.05 0.09 0.1 0.07 0.1 0.09 0.09 0 0 0.06 – – – 0.08 0.07 0.13 0 0.06 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 2 0 0 0 0 0 0 0 0 0 1 2 0 – – – 0 3 5 1 4 5
Bud 2 2 3 2 2 7 5 1 12 10 20 14 1 5 0 – – – 0 1 4 1 6 6
Bud Neck 1 2 13 12 19 14 10 14 7 4 4 11 2 2 3 – – – 1 2 0 1 4 7
Bud Site 0 1 0 0 0 0 5 1 3 4 3 4 0 0 0 – – – – – – – – –
Cell Periphery 0 0 3 1 1 4 3 2 1 2 1 2 0 0 0 – – – 0 2 4 2 1 3
Cytoplasm 32 136 220 184 281 235 306 314 120 112 99 157 69 266 246 – – – 31 104 109 134 219 205
Endoplasmic Reticulum 0 2 0 2 0 0 0 1 0 0 1 2 0 0 0 – – – 0 0 3 2 1 1
Endosome 1 0 2 1 3 5 3 4 1 0 2 4 0 4 1 – – – 4 2 8 3 3 5
Golgi 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 18 30 0 4 10 28 62 51 116 130 201 196 0 1 1 – – – 39 33 82 30 37 48
Nucleus 0 1 0 2 1 0 3 4 5 5 3 2 0 0 0 – – – 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 – – – 0 0 0 1 0 1
Nucleolus 1 1 0 0 0 0 0 1 0 0 1 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 1 1 0 1 0 3 1 0 1 1 – – – 0 0 0 0 1 0
SpindlePole 4 2 24 16 37 15 34 38 15 24 26 32 0 8 15 – – – 7 12 35 6 18 19
Vac/Vac Membrane 1 0 0 2 4 2 10 2 3 6 2 7 0 0 1 – – – 1 2 3 4 4 10
Unique Cell Count 50 154 248 211 334 283 396 396 230 237 292 345 71 280 266 92 175 268 198 315 330
Labelled Cell Count 61 177 267 226 361 311 443 434 284 298 366 432 73 289 268 92 175 268 198 315 330


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 2.8 4.8 4.4 4.9 3.8 3.9 4.4 3.3 3.3 4.5 3.4 4.0 4.6 4.4 3.8 4.5 4.5 5.2 5.1 5.0
Std Deviation (1e-4) 0.8 0.6 1.4 1.7 1.3 1.1 1.3 2.0 1.5 1.2 15.4 0.9 0.6 1.5 1.4 1.5 1.5 1.3 2.1 1.8 1.7
Intensity Change (Log2) – – – -0.11 0.02 -0.32 -0.29 -0.14 -0.53 -0.56 -0.08 -0.52 -0.26 -0.06 -0.14 -0.34 -0.08 -0.09 0.12 0.09 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.022 2.0215 2.9389 1.651 3.735 2.8977 1.3801 2.0254 1.9399 2.3796 2.3939 1.8348 -0.6949 -0.6258 -0.3841 -0.7774 -0.6553 -0.4802
Actin 0.0001 0.0046 0.0667 0.0002 0 0.0001 0.008 0.0013 0.0014 0.0215 0.0021 0.0036 0.0371 0.0007 0.053 0.0003 0.0052 0.0021
Bud 0.0002 0.0003 0.0007 0.0001 0 0 0.0053 0.0041 0.0006 0.0002 0.0002 0.0021 0.0006 0.0012 0.0006 0.0002 0.0001 0.0001
Bud Neck 0.0001 0.0003 0.0002 0.0001 0.0001 0.0002 0.0011 0.0004 0.0025 0.0004 0.0006 0.0032 0.0006 0.0005 0.0108 0.0014 0.0002 0.0017
Bud Periphery 0.0002 0.0003 0.001 0.0002 0 0 0.0252 0.0033 0.0017 0.0007 0.0003 0.0053 0.0011 0.0023 0.0011 0.0013 0.0002 0.0001
Bud Site 0.001 0.0055 0.0015 0 0 0 0.0065 0.0381 0.0018 0.0005 0.0003 0.002 0.0061 0.0049 0.0051 0.0004 0.0004 0.0001
Cell Periphery 0.0002 0.0011 0.0003 0.0005 0 0 0.0052 0.0007 0.0006 0.001 0.0005 0.0004 0.0007 0.0008 0.0005 0.0009 0.0001 0.0001
Cytoplasm 0.0227 0.121 0.0837 0.1882 0.1531 0.0997 0.0929 0.0941 0.0854 0.0457 0.1426 0.1405 0.0648 0.1433 0.0775 0.084 0.1274 0.1727
Cytoplasmic Foci 0.0032 0.0351 0.0152 0.0304 0.0183 0.0029 0.0212 0.0686 0.037 0.0111 0.023 0.0564 0.0383 0.022 0.0267 0.0061 0.0276 0.03
Eisosomes 0.0003 0.0004 0.0006 0.0001 0 0.0001 0.001 0.0004 0.0007 0.0019 0.0002 0.0003 0.0014 0.0003 0.0008 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0018 0.0018 0.003 0.0088 0.0001 0.0002 0.0037 0.0008 0.0032 0.0094 0.0017 0.0012 0.0036 0.0021 0.0039 0.0008 0.0023 0.0019
Endosome 0.0168 0.0034 0.0179 0.0338 0.0004 0.0001 0.0353 0.0038 0.0182 0.0476 0.0361 0.0092 0.0159 0.0108 0.0182 0.0011 0.0215 0.014
Golgi 0.0005 0.0008 0.0106 0.002 0 0 0.0114 0.0011 0.0041 0.0274 0.0311 0.0017 0.0076 0.0008 0.0077 0.0002 0.016 0.0022
Lipid Particles 0.001 0.0017 0.0024 0.0121 0 0 0.0061 0.0028 0.0061 0.0246 0.0166 0.0015 0.0173 0.0012 0.0065 0.0005 0.0172 0.001
Mitochondria 0.0013 0.0006 0.0116 0.0075 0 0.0002 0.0156 0.0016 0.0248 0.0059 0.0097 0.0032 0.0147 0.0047 0.0124 0.001 0.0086 0.0025
None 0.9164 0.8152 0.7732 0.7002 0.8276 0.8962 0.7524 0.7613 0.7981 0.7977 0.7074 0.7559 0.7418 0.7988 0.7513 0.8981 0.7628 0.7577
Nuclear Periphery 0.0076 0.0004 0.0019 0.0046 0 0 0.0004 0.0002 0.0013 0.0005 0.0174 0.0018 0.0156 0.0004 0.0089 0.0002 0.001 0.009
Nucleolus 0.0013 0.0017 0.0001 0.0002 0 0 0.0004 0.0017 0.0006 0.0001 0.0002 0.0003 0.0036 0.0004 0.0006 0.0005 0.0001 0.0001
Nucleus 0.0091 0.0005 0.0006 0.0006 0 0.0001 0.0006 0.0013 0.001 0.0002 0.0007 0.0027 0.0049 0.0009 0.0023 0.0006 0.0004 0.0008
Peroxisomes 0.0001 0.0019 0.0032 0.0008 0 0 0.0036 0.0116 0.0054 0.0013 0.0027 0.0057 0.0108 0.0007 0.0053 0.0002 0.0063 0.0005
Punctate Nuclear 0.0005 0.0022 0.0003 0.0005 0.0001 0 0.0003 0.0012 0.0034 0.0003 0.001 0.001 0.011 0.0005 0.0019 0.0003 0.0012 0.0012
Vacuole 0.0116 0.0011 0.0042 0.0065 0.0001 0.0001 0.0031 0.0017 0.0016 0.0012 0.0014 0.0015 0.0016 0.0026 0.0037 0.0016 0.0007 0.0014
Vacuole Periphery 0.0039 0.0001 0.0011 0.0028 0 0 0.0006 0.0001 0.0005 0.0007 0.0043 0.0004 0.001 0.0003 0.0012 0.0002 0.0004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.8168 4.6803 3.473 10.8186 6.7882 3.9245 10.0561 8.3841 11.2537 12.1083
Translational Efficiency 0.4223 0.5433 1.2791 0.401 0.588 1.5329 0.5698 0.6435 0.5552 0.3857

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
945 1341 2202 132 1506 1786 1799 1737 2451 3127 4001 1869

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 651.63 729.46 782.18 862.49 591.51 743.51 748.92 817.15 614.69 737.48 767.23 820.35
Standard Deviation 66.82 84.68 89.07 104.45 64.68 80.72 88.76 100.11 71.75 82.73 90.46 101.09
Intensity Change Log 2 — 0.162776 0.263448 0.404455 — 0.329950 0.340409 0.466198 — 0.244739 0.300580 0.434163

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000296 0.002409 0.000236 0.000859 0.000188 0.000462 0.000249 0.002121 0.000230 0.001297 0.000242 0.002032
Bud Neck 0.047719 0.055094 0.007941 0.007563 0.031181 0.059954 0.006496 0.012698 0.037557 0.057870 0.007291 0.012335
Bud Site 0.011883 0.034097 0.009127 0.018623 0.005054 0.018412 0.006576 0.033161 0.007687 0.025138 0.007980 0.032134
Cell Periphery 0.000248 0.000183 0.000175 0.000183 0.000144 0.000154 0.000142 0.000317 0.000184 0.000167 0.000160 0.000307
Cytoplasm 0.258942* 0.238554* 0.406338* 0.325249* 0.358590* 0.311069* 0.498149* 0.473380* 0.320170* 0.279971* 0.447620* 0.462918*
Cytoplasmic Foci 0.347580* 0.340858* 0.035130 0.092630 0.346263* 0.308937* 0.060032 0.065827 0.346771* 0.322626* 0.046327 0.067720
Eisosomes 0.000368 0.000236 0.000046 0.000145 0.000234 0.000201 0.000065 0.000083 0.000286 0.000216 0.000055 0.000088
Endoplasmic Reticulum 0.001669 0.000756 0.001218 0.003488 0.000752 0.000541 0.001366 0.001747 0.001106 0.000633 0.001285 0.001870
Endosome 0.048120 0.052565 0.004237 0.016570 0.018622 0.037978 0.005980 0.009702 0.029995 0.044234 0.005021 0.010188
Golgi 0.010064 0.023092 0.000334 0.000312 0.004429 0.009259 0.000390 0.002376 0.006602 0.015191 0.000359 0.002230
Lipid Particles 0.029290 0.008295 0.003250 0.004641 0.024291 0.011572 0.002895 0.002949 0.026219 0.010167 0.003090 0.003068
Mitochondria 0.021683 0.041685 0.001290 0.000370 0.013211 0.019573 0.000983 0.002107 0.016477 0.029056 0.001152 0.001985
Mitotic Spindle 0.001528 0.009511 0.005398 0.043240 0.001319 0.005049 0.004825 0.020870 0.001399 0.006963 0.005141 0.022450
None 0.002071 0.001386 0.006236 0.007899 0.003229 0.001829 0.004763 0.003645 0.002783 0.001639 0.005574 0.003945
Nuclear Periphery 0.000823 0.000270 0.002121 0.001722 0.000788 0.000370 0.001325 0.001178 0.000802 0.000327 0.001763 0.001217
Nuclear Periphery Foci 0.000358 0.000433 0.000589 0.003033 0.000241 0.000208 0.000755 0.001023 0.000286 0.000304 0.000663 0.001165
Nucleolus 0.001758 0.001705 0.002237 0.001390 0.001586 0.001977 0.001412 0.001037 0.001652 0.001860 0.001866 0.001062
Nucleus 0.103874 0.052923 0.480181* 0.191938* 0.135082* 0.094071 0.328602* 0.223647* 0.123050 0.076425 0.412025* 0.221407*
Peroxisomes 0.034966 0.061173 0.001669 0.006317 0.017342 0.042906 0.002167 0.007713 0.024137 0.050740 0.001893 0.007615
Vacuole 0.073710 0.070971 0.031716 0.273460* 0.035935 0.073397 0.072259 0.132828 0.050499 0.072356 0.049946 0.142760
Vacuole Periphery 0.003051 0.003805 0.000530 0.000367 0.001518 0.002080 0.000570 0.001592 0.002109 0.002819 0.000548 0.001506

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.20 0.57 -1.91 1.98 -2.03 -5.00 -1.35 -2.35 -1.85 -2.17 -3.75 -0.40 -2.36 -0.30 -2.30
Bud Neck -1.69 11.97 10.13 12.94 -0.26 -8.08 11.22 7.96 15.42 -4.82 -7.42 16.18 12.34 19.91 -4.41
Bud Site -8.49 1.38 -1.24 3.25 -1.91 -8.59 -1.32 -9.17 -2.65 -8.12 -12.42 -0.09 -7.90 0.22 -7.83
Cell Periphery 1.61 3.46 2.63 0.22 -0.07 -0.45 0.77 -1.48 -1.28 -1.64 0.94 2.52 -0.73 -1.05 -1.30
Cytoplasm 1.91 -13.79 1.19 0.34 7.20 5.06 -11.97 -6.07 -11.27 5.81 5.66 -15.73 -9.98 -15.18 3.13
Cytoplasmic Foci 0.86 41.34 26.76 27.52 -2.00 4.52 43.87 44.11 41.37 0.11 3.94 61.27 55.31 55.00 -4.03
Eisosomes 5.14 14.08 8.75 5.23 -8.11 2.47 15.11 13.18 12.22 -4.52 5.35 20.59 17.05 15.56 -9.47
Endoplasmic Reticulum 3.11 0.72 -5.12 -6.87 -5.82 1.89 -8.08 -11.79 -13.39 -4.45 3.57 -3.41 -8.78 -15.34 -7.53
Endosome -1.02 14.11 6.72 7.83 -2.58 -8.58 9.78 7.67 15.12 -2.23 -6.56 17.00 13.62 20.44 -3.91
Golgi -6.30 7.98 8.05 13.99 0.47 -5.37 10.65 5.29 8.55 -2.48 -8.51 11.84 8.00 14.77 -2.51
Lipid Particles 9.73 12.50 11.96 5.68 -1.67 7.20 13.32 13.22 10.40 0.05 11.75 18.11 18.02 12.38 0.47
Mitochondria -5.26 9.04 9.45 13.85 4.52 -3.37 10.23 9.56 12.68 -2.28 -6.49 13.42 12.92 17.96 -1.35
Mitotic Spindle -4.19 -3.12 -2.85 -2.12 -2.50 -3.18 -2.96 -7.56 -5.65 -5.61 -5.28 -4.50 -8.11 -5.36 -6.31
None 0.85 -4.27 -1.38 -1.87 0.92 2.41 -1.93 0.13 -1.87 1.80 2.46 -4.52 -0.63 -2.50 2.93
Nuclear Periphery 3.33 -7.34 -5.32 -7.89 -0.83 2.76 -5.17 -6.35 -17.44 -2.04 4.20 -9.08 -8.23 -19.90 1.20
Nuclear Periphery Foci -0.88 -2.29 -5.59 -5.43 -5.15 1.21 -8.84 -9.40 -9.70 -2.66 -0.63 -6.54 -10.23 -9.65 -5.40
Nucleolus -0.31 -2.21 -0.31 -0.22 0.39 -1.53 1.06 1.85 2.73 0.84 -1.44 -1.64 1.94 2.83 3.07
Nucleus 8.73 -46.79 -4.16 -6.91 15.27 7.38 -27.02 -14.21 -21.47 12.75 11.42 -51.99 -17.33 -26.54 26.96
Peroxisomes -6.20 11.97 9.99 16.58 -1.96 -10.13 13.67 7.70 14.87 -4.90 -11.68 17.34 11.70 21.52 -5.36
Vacuole 0.25 7.87 -13.28 -13.41 -14.74 -12.29 -15.32 -29.57 -21.31 -17.50 -8.51 -4.74 -28.89 -24.88 -26.87
Vacuole Periphery -1.70 9.61 10.23 10.17 2.19 -2.31 5.59 1.88 3.48 -1.68 -3.07 10.94 4.38 6.35 -1.71
Standard name
Human Ortholog
Description Mitochondrial 3-hydroxyacyl-thioester dehydratase; involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology
Localization
Cell Percentages mitochondrion (11%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Htd2

Htd2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Htd2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available