Standard name
Human Ortholog
Description Protein involved in bud growth; involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; SBE22 has a paralog, SBE2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.98 0.99 0.96 0.91 0.81 0.84 0.77 0.78 0.74 0.76 1.0 0.99 0.98 0.98 0.97 0.96 0.93 0.94 0.92 0.91 0.91 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.31 0.42 0.32 0.48 0.51 0.5 0.49 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 0 2 2
Bud 1 6 0 0 0 3 6 8 4 9 10 10 0 0 0 0 0 0 1 1 3 0 0 7
Bud Neck 0 1 4 0 1 0 1 2 0 0 1 0 1 1 0 1 0 0 2 2 1 0 0 2
Bud Site 0 0 0 0 0 0 0 1 0 2 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 15 9 6 6 5 2 2 3 3 3 3 4 3 1 0 21 10 8 0 0 1 0 1 0
Cytoplasm 510 458 382 284 377 486 531 572 324 452 343 346 211 72 116 460 302 180 492 452 353 135 190 218
Endoplasmic Reticulum 3 7 4 1 1 0 0 1 3 0 1 0 0 0 2 30 24 10 2 2 1 1 1 4
Endosome 1 1 0 0 4 3 0 2 0 0 0 0 0 0 1 2 2 1 5 3 0 0 2 2
Golgi 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 2 5 4 2 0 0 0
Mitochondria 4 7 6 10 19 168 275 216 203 295 231 224 0 0 0 15 9 6 2 6 5 4 4 9
Nucleus 0 0 0 0 0 0 2 2 0 0 1 1 0 1 0 3 3 0 0 2 0 0 0 0
Nuclear Periphery 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 5 4 2 4 7 3 0 1 0 0 0 0 4 0 1 0 0 1
Vac/Vac Membrane 3 3 0 0 3 2 5 4 0 5 0 2 0 0 0 1 1 0 6 1 2 1 2 2
Unique Cell Count 518 479 389 288 391 536 654 681 423 581 463 457 211 73 118 470 312 187 528 484 386 149 210 254
Labelled Cell Count 539 495 403 301 411 664 829 817 539 771 599 591 215 76 119 535 354 208 528 484 386 149 210 254


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.5 4.3 4.0 3.9 3.1 2.8 3.3 2.8 2.8 2.8 2.9 6.2 6.3 6.1 5.4 6.1 6.7 6.2 6.3 6.5
Std Deviation (1e-4) 0.7 0.7 1.0 0.8 1.1 0.9 1.0 1.0 0.6 0.7 0.8 1.0 0.9 1.1 0.7 1.2 1.1 1.6 1.1 1.5 1.7
Intensity Change (Log2) – – – -0.09 -0.14 -0.48 -0.59 -0.38 -0.61 -0.64 -0.59 -0.57 0.53 0.55 0.51 0.35 0.52 0.65 0.54 0.57 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.4 1.5 0
Cytoplasm 0.4 -1.5 -4.7 -8.0 -7.2 -9.1 -9.0 -9.8 -9.4 2.0 0.3 0.1 -0.3 -1.2 -1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 4.0 4.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 1.6 1.2 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0789 1.8183 1.4263 1.1634 1.3222 1.6568 3.4386 4.0517 3.4568 3.2888 2.9141 4.1671 3.5917 4.2154 3.8815 4.125 4.1342 4.3398
Actin 0.0143 0.0014 0.0037 0.0018 0.0007 0.0016 0.053 0.0009 0.0047 0.0243 0.0001 0.0091 0.001 0.001 0.0007 0.0011 0.0007 0.0015
Bud 0.001 0.0023 0.0013 0.0003 0.0001 0.0004 0.001 0.0009 0.0008 0.0263 0.0002 0.0016 0.0018 0.001 0.0014 0.0041 0.0002 0.0003
Bud Neck 0.0008 0.0004 0.0004 0.0002 0.0001 0.0012 0.0039 0.0002 0.0006 0.0014 0.0001 0.0086 0.0004 0.0004 0.001 0.0009 0.0003 0.0017
Bud Periphery 0.0016 0.0012 0.0015 0.0002 0.0002 0.0007 0.0021 0.0009 0.001 0.044 0.0002 0.003 0.0028 0.0009 0.002 0.015 0.0003 0.0005
Bud Site 0.0014 0.0187 0.0059 0.0002 0.0001 0.0002 0.0049 0.0057 0.0034 0.0626 0 0.0003 0.0051 0.0102 0.0061 0.0047 0.0001 0.0001
Cell Periphery 0.0003 0.0005 0.0002 0.0001 0.0001 0.0001 0.0008 0.0007 0.0004 0.0006 0.0001 0.0002 0.0006 0.0004 0.0003 0.0009 0.0001 0.0001
Cytoplasm 0.6131 0.7638 0.6586 0.6564 0.7009 0.7874 0.3894 0.3986 0.4365 0.3227 0.6057 0.5178 0.539 0.6841 0.5709 0.5202 0.6071 0.6558
Cytoplasmic Foci 0.0107 0.0141 0.0167 0.0212 0.0053 0.0086 0.0317 0.0068 0.0129 0.0271 0.0074 0.0079 0.0116 0.0105 0.0109 0.0119 0.0096 0.0048
Eisosomes 0.0003 0.0002 0.0001 0.0001 0.0001 0 0.0004 0.0002 0.0001 0.0003 0 0.0001 0.0002 0.0001 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0057 0.0025 0.0023 0.0038 0.0015 0.0024 0.0063 0.0017 0.0025 0.0018 0.0042 0.0014 0.0017 0.0016 0.0021 0.0012 0.0024 0.0007
Endosome 0.006 0.0061 0.0056 0.0103 0.0025 0.004 0.0437 0.0023 0.0123 0.0247 0.0109 0.0048 0.002 0.0035 0.0038 0.0055 0.0068 0.0014
Golgi 0.0012 0.0006 0.0007 0.0009 0.0002 0.0003 0.0169 0.0003 0.0039 0.0285 0.0003 0.0016 0.0003 0.0008 0.0003 0.0004 0.0002 0.0001
Lipid Particles 0.0014 0.0003 0.0007 0.0025 0.0002 0.0001 0.0125 0.0003 0.0025 0.0056 0.0003 0.0002 0.0003 0.0001 0.0002 0.0001 0.0002 0
Mitochondria 0.0006 0.0005 0.0005 0.0004 0.0002 0.0002 0.0032 0.0004 0.0012 0.0737 0.0003 0.0021 0.0003 0.0004 0.0005 0.0006 0.0003 0.0002
None 0.3286 0.1824 0.2938 0.2946 0.2856 0.1904 0.3876 0.5743 0.5033 0.331 0.3631 0.4393 0.4284 0.2774 0.3925 0.4276 0.3661 0.3313
Nuclear Periphery 0.0018 0.0005 0.0006 0.0008 0.0003 0.0003 0.0073 0.0006 0.0006 0.0008 0.0012 0.0002 0.0003 0.0004 0.0006 0.0004 0.0007 0.0001
Nucleolus 0.0004 0.0002 0.0001 0.0001 0 0 0.001 0.0002 0.0002 0.0003 0 0 0.0002 0.0002 0.0004 0.0002 0.0001 0
Nucleus 0.0049 0.0016 0.0009 0.0009 0.0005 0.0006 0.0035 0.001 0.0007 0.0011 0.0005 0.0003 0.0011 0.0013 0.0023 0.0016 0.0011 0.0004
Peroxisomes 0.0008 0.0002 0.0034 0.0008 0.0001 0.0002 0.0085 0.0002 0.0099 0.0189 0.0001 0.0004 0.0005 0.0002 0.0002 0.0002 0.0001 0.0001
Punctate Nuclear 0.0027 0.001 0.001 0.0008 0.0001 0.0004 0.0174 0.0009 0.0005 0.0007 0.0002 0.0002 0.0008 0.0035 0.0012 0.0004 0.0004 0.0002
Vacuole 0.0022 0.0015 0.0017 0.0031 0.001 0.0007 0.0035 0.0028 0.0017 0.0028 0.0044 0.0007 0.0016 0.0017 0.0022 0.0025 0.0024 0.0005
Vacuole Periphery 0.0002 0.0002 0.0002 0.0005 0.0002 0.0001 0.0013 0.0002 0.0003 0.0008 0.0009 0.0002 0.0001 0.0002 0.0002 0.0003 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.6922 23.8981 20.5937 45.7692 39.1336 29.7353 30.3991 25.5151 36.5921 43.9911
Translational Efficiency 0.6938 0.7026 0.6255 0.4527 0.5754 0.6729 0.5376 0.4902 0.5906 0.5212

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
199 1130 1640 1341 1877 1877 2166 1459 2076 3007 3806 2800

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 842.02 755.38 863.86 836.81 741.06 806.10 869.98 869.08 750.74 787.04 867.34 853.62
Standard Deviation 133.34 111.87 119.65 117.64 557.21 100.32 98.89 114.06 532.27 107.65 108.36 116.91
Intensity Change Log 2 — -0.156652 0.036943 -0.008954 — 0.121368 0.231392 0.229899 — -0.019820 0.131237 0.107790

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000883 0.000353 0.000085 0.003926 0.000409 0.000633 0.000097 0.000943 0.000455 0.000528 0.000092 0.002372
Bud Neck 0.005004 0.020911 0.002784 0.009918 0.018834 0.026719 0.002293 0.009242 0.017508 0.024537 0.002504 0.009566
Bud Site 0.023292 0.020086 0.007712 0.105442* 0.008386 0.018981 0.006138 0.052159 0.009815 0.019396 0.006816 0.077678
Cell Periphery 0.000722 0.000131 0.000187 0.000236 0.000134 0.000129 0.000057 0.000150 0.000191 0.000130 0.000113 0.000191
Cytoplasm 0.296751* 0.503370* 0.643519* 0.520556* 0.530425* 0.532308* 0.763747* 0.628431* 0.508026* 0.521433* 0.711941* 0.576767*
Cytoplasmic Foci 0.116094 0.238937* 0.042475 0.027598 0.254740* 0.196055* 0.017257 0.028086 0.241450* 0.212169* 0.028123 0.027852
Eisosomes 0.000136 0.000119 0.000059 0.000045 0.000225 0.000175 0.000020 0.000041 0.000217 0.000154 0.000037 0.000043
Endoplasmic Reticulum 0.002327 0.000809 0.000609 0.001302 0.000651 0.000382 0.000541 0.000680 0.000812 0.000543 0.000570 0.000977
Endosome 0.045849 0.037031 0.004081 0.008722 0.017534 0.026479 0.001468 0.005637 0.020248 0.030444 0.002594 0.007115
Golgi 0.045890 0.006789 0.000171 0.007919 0.004764 0.004780 0.000099 0.003758 0.008706 0.005535 0.000130 0.005751
Lipid Particles 0.002069 0.004544 0.001048 0.000901 0.005955 0.003686 0.000471 0.000616 0.005583 0.004008 0.000720 0.000753
Mitochondria 0.004984 0.004010 0.000324 0.009212 0.006251 0.004239 0.000254 0.005627 0.006129 0.004153 0.000284 0.007344
Mitotic Spindle 0.027811 0.003068 0.002294 0.064764 0.000575 0.005989 0.004441 0.035629 0.003186 0.004891 0.003516 0.049583
None 0.000236 0.003366 0.010590 0.007063 0.001126 0.001429 0.003345 0.003015 0.001041 0.002157 0.006467 0.004954
Nuclear Periphery 0.000372 0.000243 0.000447 0.000718 0.000154 0.000187 0.000513 0.000469 0.000175 0.000208 0.000485 0.000588
Nuclear Periphery Foci 0.001310 0.000569 0.001118 0.001239 0.000178 0.000242 0.000834 0.000658 0.000286 0.000365 0.000956 0.000937
Nucleolus 0.000167 0.001178 0.000493 0.000222 0.000410 0.000822 0.000101 0.000223 0.000386 0.000956 0.000270 0.000223
Nucleus 0.031671 0.039643 0.171588* 0.072312 0.064136 0.056718 0.112735 0.083265 0.061024 0.050302 0.138094* 0.078019
Peroxisomes 0.003408 0.008021 0.000628 0.003408 0.003587 0.006849 0.000302 0.002691 0.003570 0.007290 0.000442 0.003034
Vacuole 0.376932* 0.103498 0.109388 0.147833 0.079956 0.111152 0.085164 0.136162 0.108424 0.108276 0.095602 0.141751
Vacuole Periphery 0.014093 0.003324 0.000400 0.006664 0.001569 0.002046 0.000125 0.002518 0.002769 0.002526 0.000244 0.004503

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.48 3.31 -1.71 -2.15 -2.60 -0.63 1.10 -0.51 0.32 -4.17 -0.37 1.33 -1.72 -1.63 -3.33
Bud Neck -9.53 1.19 -2.99 8.07 -4.82 -4.14 15.04 9.19 11.62 -5.13 -4.67 14.97 9.97 14.34 -7.07
Bud Site -0.60 3.03 -5.50 -6.11 -9.35 -6.32 3.47 -6.80 -2.46 -8.31 -7.46 3.51 -10.76 -6.41 -12.42
Cell Periphery 1.29 1.13 1.25 -0.52 1.48 0.67 9.11 3.28 2.88 -5.45 1.43 2.45 1.87 1.27 -1.44
Cytoplasm -8.73 -15.02 -6.71 3.73 16.06 -0.03 -14.98 0.81 0.80 13.66 -1.30 -18.19 2.26 3.72 21.16
Cytoplasmic Foci -15.12 5.79 8.33 35.01 9.95 9.24 50.14 47.81 37.20 -5.26 4.84 46.77 47.81 50.81 4.06
Eisosomes -0.33 4.01 4.36 9.21 1.34 2.03 12.30 10.98 6.33 -9.11 3.19 11.90 11.22 8.09 -5.28
Endoplasmic Reticulum 1.63 1.96 0.59 -3.63 -5.80 2.71 -2.37 -2.69 -9.19 -0.42 1.91 -0.35 -3.44 -7.89 -5.01
Endosome -1.70 4.59 4.58 13.94 -0.00 -5.33 17.55 14.49 16.50 -3.81 -7.55 16.44 14.82 21.38 -2.70
Golgi 2.16 2.94 2.63 4.09 -3.11 0.10 12.97 4.51 4.03 -2.02 1.30 7.52 4.64 5.42 -3.67
Lipid Particles -5.93 3.05 2.30 8.15 -1.99 3.90 10.52 10.13 9.33 -3.89 3.01 10.37 9.92 12.21 -4.12
Mitochondria -0.95 2.22 -0.48 0.50 -3.58 1.62 5.16 3.02 2.37 -3.54 1.61 5.34 2.62 1.92 -4.99
Mitotic Spindle 1.97 2.10 -2.32 -8.39 -8.67 -3.95 -5.04 -7.22 -4.54 -5.59 -2.56 -1.63 -10.56 -8.99 -10.18
None -2.93 -7.31 -4.10 -0.83 3.06 -1.03 -10.13 -7.66 -6.57 -1.85 -2.54 -8.83 -6.06 -3.31 2.27
Nuclear Periphery 3.81 0.15 -10.60 -14.50 -10.17 -1.71 -23.79 -18.02 -17.18 -2.59 -1.18 -15.18 -22.11 -21.53 -9.92
Nuclear Periphery Foci 3.63 -0.21 0.26 -5.96 0.46 -1.94 -14.26 -7.69 -5.87 3.48 -2.79 -8.86 -11.95 -9.07 1.86
Nucleolus -5.36 -6.53 -3.77 4.80 4.25 -3.03 9.87 0.19 2.62 -2.55 -5.02 3.71 0.99 5.12 -1.00
Nucleus -0.48 -20.92 -14.43 -17.64 7.20 2.36 -23.08 -15.82 -17.05 4.00 4.51 -29.98 -19.85 -23.85 7.90
Peroxisomes -6.14 2.47 1.00 7.97 -2.15 -4.82 12.71 5.64 7.97 -2.65 -7.13 12.25 6.30 11.11 -3.48
Vacuole 16.04 14.65 7.33 -20.58 -16.92 -8.20 -14.71 -23.14 -17.55 -11.71 0.91 -6.12 -23.57 -26.86 -20.04
Vacuole Periphery 1.28 2.16 1.57 1.08 -2.59 -1.58 8.99 1.42 2.31 -2.27 -0.74 5.43 1.08 1.88 -3.42
Standard name
Human Ortholog
Description Protein involved in bud growth; involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; SBE22 has a paralog, SBE2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (77%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sbe22

Sbe22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sbe22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available