Standard name
Human Ortholog
Description Endosomal protein of unknown function; contains a phox (PX) homology domain; binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.07 0 0.07 0.06 0.06 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0 0.25 0.18 0.15 0.25 0.22 0.26 0.29 0.39 0.05 0.07 0.08 0.08 0.06 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0.72 0.37 0.31 0.55 0.64 0.33 0.52 0.16 0.26 0.11 0.15 0.79 0.78 0.78 0.79 0.76 0.68 0.77 0.72 0.71 0.86 0.87 0.77
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.35 0.31 0.17 0 0.66 0.07 0.48 0.41 0.46 0.39 0 0 0 0.11 0 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.13 0.31 0.1 0 0 0 0 0 0 0 0.12 0.09 0.08 0.16 0.18 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.13 0.1 0.38 0.17 0.22 0.07 0.16 0.16 0.15 0.06 0.12 0.16 0.18 0.16 0.29 0.29 0.14 0 0 0.09 0.05 0 0
Vac/Vac Membrane 0.42 0.34 0.25 0.12 0.27 0.2 0.2 0.17 0.06 0.1 0.07 0.44 0.31 0.38 0.34 0.47 0.43 0.09 0.11 0.06 0 0 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 1 4 0 0 0 1
Bud 0 0 0 0 1 4 5 4 5 4 11 0 3 0 0 0 0 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0
Cell Periphery 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 11 0 10 24 9 35 13 21 18 39 10 23 23 3 1 8 0 4 0 0 0 3
Endoplasmic Reticulum 1 3 0 0 0 0 0 0 0 1 2 1 5 3 2 0 3 1 0 0 0 2 2
Endosome 141 50 5 22 84 20 72 9 21 7 15 148 259 228 30 13 43 171 114 34 152 270 208
Golgi 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 2 3 0 0 0 2 3
Mitochondria 23 47 5 7 3 40 10 28 33 29 39 3 13 2 4 0 6 5 4 2 2 7 6
Nucleus 1 0 0 1 1 1 2 0 3 1 0 1 0 0 0 0 1 1 1 0 0 0 1
Nuclear Periphery 2 3 0 0 0 0 0 0 0 0 0 3 4 0 0 0 1 0 0 0 1 1 2
Nucleolus 33 17 5 4 1 1 1 2 2 1 2 23 29 24 6 3 4 1 0 1 1 0 1
Peroxisomes 1 0 1 4 1 0 1 0 3 0 1 0 6 5 0 0 0 2 1 0 0 0 2
SpindlePole 26 14 6 7 29 4 22 9 12 4 12 30 58 46 11 5 9 9 6 4 9 9 7
Vac/Vac Membrane 83 46 4 5 36 12 28 10 5 6 7 83 101 110 13 8 27 20 17 2 5 11 26
Unique Cell Count 196 134 16 40 131 61 138 58 81 63 100 187 331 291 38 17 63 224 160 49 177 313 273
Labelled Cell Count 318 191 26 61 180 91 177 76 106 72 129 303 502 442 70 30 104 224 160 49 177 313 273


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 5.4 5.9 6.1 7.7 4.4 5.7 5.1 5.0 5.1 5.2 6.5 5.9 7.2 7.5 9.1 8.2 5.8 5.8 6.4
Std Deviation (1e-4) 0.9 0.9 1.5 2.5 4.0 1.1 1.3 1.4 1.5 1.2 1.6 1.3 1.0 1.6 1.0 1.7 1.8 1.6 1.5 2.0
Intensity Change (Log2) 0.07 0.4 -0.41 -0.04 -0.19 -0.24 -0.19 -0.17 0.15 0.02 0.29 0.35 0.64 0.49 -0.02 -0.0 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 1.6 2.5 1.6 0 0 0 0 4.3 4.3 4.3 3.4 2.6 2.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 1.2 0.7 1.1 0.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuc
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 -0.6 -0.5 0
Vac
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 3.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 1.5 0 1.0 0.7 1.3 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4805 0.8252 0.1203 -0.1059 -0.5447 0.0467 1.8707 2.1019 1.5971 1.7761 1.4961 1.6576 3.5184 4.577 4.2199 3.9947 3.4047 4.4644
Actin 0.0004 0.0001 0.0029 0.0138 0.0002 0.0001 0.0491 0 0.0124 0.0498 0.008 0.0002 0.0062 0 0.0001 0 0.0014 0.0001
Bud 0 0.0002 0.0003 0.005 0.0001 0.0004 0.0008 0.0001 0.0003 0.0002 0.0003 0 0 0 0.0001 0 0 0
Bud Neck 0.0005 0.0017 0.0005 0.0004 0.0002 0.0006 0.0059 0.0007 0.0007 0.0012 0.0006 0.0005 0.0004 0.0002 0.0002 0.0001 0.0002 0.0004
Bud Periphery 0.0001 0.0003 0.0003 0.0015 0.0001 0.0002 0.0011 0.0001 0.0002 0.0003 0.0003 0 0.0001 0 0 0 0 0.0001
Bud Site 0.0002 0.0012 0.0013 0.0016 0.0002 0.0002 0.009 0.0011 0.0019 0.0006 0.0007 0.0001 0.0003 0.0001 0.0002 0 0.0004 0.0003
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0005 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0.0001
Cytoplasm 0.0042 0.0067 0.0049 0.0076 0.001 0.0052 0.0173 0.011 0.0058 0.0022 0.0333 0.002 0.0016 0.0019 0.0047 0.0009 0.0024 0.004
Cytoplasmic Foci 0.149 0.1598 0.1566 0.148 0.2376 0.1542 0.1277 0.1625 0.1568 0.0806 0.157 0.1472 0.1406 0.1069 0.1412 0.1462 0.1292 0.1395
Eisosomes 0.0001 0 0 0.0001 0 0 0.0003 0 0 0.0004 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0008 0.0016 0.0008 0.0007 0.0005 0.0005 0.0062 0.0015 0.0007 0.001 0.0021 0.0006 0.0009 0.0008 0.0006 0.0002 0.0008 0.0004
Endosome 0.5463 0.5389 0.5522 0.5673 0.4912 0.5087 0.4802 0.5626 0.5643 0.711 0.5732 0.5653 0.5357 0.6044 0.5519 0.6174 0.6862 0.4981
Golgi 0.0367 0.0377 0.0589 0.0421 0.0549 0.0472 0.0524 0.0346 0.0578 0.0382 0.097 0.0662 0.0477 0.0415 0.058 0.0481 0.0601 0.055
Lipid Particles 0.1583 0.1582 0.1112 0.1189 0.1382 0.2038 0.12 0.1151 0.1176 0.0482 0.0749 0.1494 0.1723 0.161 0.1487 0.1055 0.0742 0.2171
Mitochondria 0.0042 0.0033 0.0103 0.0036 0.0037 0.0055 0.0188 0.001 0.0026 0.0011 0.0178 0.0044 0.0019 0.0011 0.009 0.0138 0.0013 0.0038
None 0.0196 0.003 0.0124 0.0068 0.0001 0.0028 0.0064 0.0039 0.0005 0.0002 0.0065 0.0003 0.006 0.0002 0.0098 0.0001 0.0036 0.0005
Nuclear Periphery 0.0029 0.004 0.0016 0.0012 0.0006 0.0015 0.0172 0.0023 0.0011 0.0013 0.0004 0.0084 0.0031 0.0026 0.0011 0.0002 0.002 0.001
Nucleolus 0.005 0.001 0.0021 0.0014 0.0001 0.0014 0.0025 0.0145 0.0017 0.0002 0.0002 0.0007 0.0037 0.0004 0.0005 0.0001 0.0004 0.0043
Nucleus 0.0008 0.0017 0.0007 0.0031 0.0001 0.0007 0.0032 0.0022 0.001 0.0002 0.0002 0.002 0.0012 0.0003 0.0003 0.0001 0.0005 0.0011
Peroxisomes 0.0395 0.0298 0.0466 0.0343 0.0641 0.0374 0.0399 0.0155 0.0267 0.0075 0.015 0.0204 0.0349 0.0104 0.0351 0.0575 0.0193 0.0448
Punctate Nuclear 0.0024 0.0032 0.0018 0.0161 0.0013 0.0027 0.0108 0.0063 0.0092 0.0003 0.0007 0.0081 0.0087 0.0011 0.0084 0.0004 0.0059 0.0026
Vacuole 0.0234 0.0375 0.0273 0.0214 0.0036 0.0196 0.0245 0.0546 0.0339 0.0419 0.0087 0.0164 0.027 0.0531 0.0238 0.0061 0.0081 0.0195
Vacuole Periphery 0.0057 0.0101 0.007 0.0049 0.0021 0.0072 0.0063 0.0103 0.0047 0.0135 0.0028 0.0076 0.0076 0.0138 0.0062 0.0034 0.0036 0.0072

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.5661 4.5878 3.4043 5.6866 4.436 10.2585 8.5043 5.7398 6.2647 5.366
Translational Efficiency 2.2068 1.557 1.4145 1.0936 1.5262 0.9392 0.9195 1.5438 1.7156 1.6038

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
981 1465 367 628 1554 2289 1232 2081 2535 3754 1599 2709

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 718.53 794.06 1044.96 964.12 720.98 833.72 896.87 1008.33 720.03 818.24 930.86 998.08
Standard Deviation 576.37 145.53 128.24 140.50 83.52 107.85 121.80 127.71 364.47 125.43 138.14 132.11
Intensity Change Log 2 0.144200 0.540327 0.424164 0.209604 0.314940 0.483937 0.177328 0.431839 0.454411

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000469 0.000203 0.001350 0.000272 0.000079 0.000131 0.000090 0.000173 0.000230 0.000159 0.000379 0.000196
Bud Neck 0.013920 0.005484 0.011781 0.004758 0.004548 0.003718 0.004198 0.003026 0.008175 0.004407 0.005939 0.003427
Bud Site 0.008554 0.006523 0.010762 0.015918 0.002797 0.003703 0.009110 0.008850 0.005025 0.004803 0.009489 0.010489
Cell Periphery 0.000270 0.000050 0.000071 0.000037 0.000057 0.000048 0.000029 0.000027 0.000140 0.000049 0.000039 0.000029
Cytoplasm 0.025937 0.017327 0.001694 0.009100 0.011807 0.003979 0.006004 0.008558 0.017275 0.009188 0.005015 0.008684
Cytoplasmic Foci 0.182384* 0.112453 0.106456 0.130075 0.108008 0.074736 0.125126 0.106695 0.136790 0.089455 0.120841 0.112115
Eisosomes 0.000217 0.000023 0.000032 0.000010 0.000037 0.000018 0.000013 0.000008 0.000106 0.000020 0.000018 0.000008
Endoplasmic Reticulum 0.002726 0.000521 0.001084 0.000199 0.000457 0.000266 0.000372 0.000246 0.001335 0.000365 0.000535 0.000235
Endosome 0.418447* 0.671393* 0.487364* 0.584776* 0.683409* 0.749875* 0.622193* 0.608657* 0.580873* 0.719248* 0.591247* 0.603121*
Golgi 0.138183* 0.045576 0.031016 0.038607 0.060712 0.046043 0.027220 0.042731 0.090692 0.045861 0.028091 0.041775
Lipid Particles 0.021476 0.009686 0.007098 0.003307 0.013945 0.013537 0.003429 0.001652 0.016860 0.012034 0.004271 0.002036
Mitochondria 0.068722 0.009669 0.002681 0.002294 0.026212 0.016969 0.001932 0.003600 0.042663 0.014120 0.002104 0.003297
Mitotic Spindle 0.000795 0.007848 0.086388 0.029252 0.003148 0.007829 0.043171 0.028362 0.002238 0.007837 0.053090 0.028568
None 0.000975 0.000503 0.000038 0.000051 0.000139 0.000251 0.000053 0.000058 0.000463 0.000349 0.000050 0.000056
Nuclear Periphery 0.000218 0.000399 0.003097 0.000501 0.000197 0.000380 0.000589 0.000626 0.000205 0.000387 0.001164 0.000597
Nuclear Periphery Foci 0.001378 0.002626 0.025696 0.005074 0.000674 0.002959 0.008432 0.003534 0.000946 0.002829 0.012394 0.003891
Nucleolus 0.000747 0.001940 0.003668 0.000805 0.001128 0.001574 0.003299 0.000788 0.000981 0.001717 0.003384 0.000792
Nucleus 0.007096 0.003789 0.002533 0.000484 0.001122 0.001414 0.001673 0.000408 0.003434 0.002341 0.001870 0.000425
Peroxisomes 0.021435 0.012335 0.040512 0.020362 0.014064 0.010704 0.018716 0.011161 0.016916 0.011340 0.023719 0.013294
Vacuole 0.047594 0.074018 0.149820 0.141044 0.049174 0.047537 0.110501 0.148768 0.048562 0.057871 0.119525 0.146977
Vacuole Periphery 0.038456 0.017635 0.026858 0.013074 0.018285 0.014331 0.013850 0.022072 0.026091 0.015620 0.016836 0.019986

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.95 -1.11 1.14 -0.61 1.34 -1.21 -1.17 -1.35 -0.60 -1.11 1.19 -0.84 0.29 -0.63 0.94
Bud Neck 4.19 0.66 4.41 0.35 4.16 1.12 -0.01 1.99 0.66 2.95 4.16 2.01 5.24 1.36 5.02
Bud Site 1.37 -1.58 -3.19 -4.39 -1.99 -1.42 -6.73 -8.05 -6.08 0.07 0.26 -5.24 -6.53 -7.13 -1.00
Cell Periphery 7.96 6.95 8.38 1.82 3.97 0.72 3.12 3.46 2.88 0.99 7.00 8.19 9.11 3.85 3.49
Cytoplasm 2.43 8.46 4.80 2.98 -4.65 4.72 2.55 1.14 -4.76 -1.78 4.78 6.92 4.38 -0.41 -3.29
Cytoplasmic Foci 11.47 9.40 7.44 -2.38 -2.73 8.56 -2.94 0.66 -9.04 3.78 13.73 4.04 7.00 -7.00 2.03
Eisosomes 7.76 7.28 8.25 5.41 6.19 6.14 7.50 9.83 8.15 6.58 8.66 8.78 9.81 10.03 8.82
Endoplasmic Reticulum 4.84 2.56 5.49 2.24 2.52 2.53 0.58 1.62 -0.31 1.25 5.28 3.66 5.59 1.09 2.74
Endosome -21.25 -3.34 -10.33 7.84 -4.98 -7.43 7.12 8.93 18.53 0.84 -18.08 0.31 -1.40 18.43 -1.64
Golgi 14.87 15.90 15.65 2.42 -1.58 4.40 11.38 5.89 1.64 -7.50 13.83 19.96 15.41 2.49 -7.18
Lipid Particles 5.10 6.26 8.75 5.03 3.26 0.18 7.27 8.82 8.84 4.73 3.23 10.31 12.74 10.60 5.65
Mitochondria 13.20 14.89 15.16 6.13 0.37 3.73 11.26 10.50 10.49 -3.57 12.22 18.49 18.01 11.98 -2.72
Mitotic Spindle -5.64 -7.88 -6.96 -5.06 4.83 -3.55 -11.25 -10.45 -8.64 4.14 -6.00 -13.79 -12.90 -10.01 6.21
None 0.99 2.15 2.12 3.18 -0.70 -1.40 1.46 1.37 2.93 -0.12 0.55 2.38 2.35 4.27 -0.31
Nuclear Periphery -2.09 -5.65 -2.72 -1.11 4.85 -2.65 -6.00 -4.37 -2.43 0.33 -3.37 -7.70 -5.07 -2.63 3.99
Nuclear Periphery Foci -1.71 -6.29 -2.73 -1.90 5.01 -4.55 -8.02 -5.58 -1.11 4.60 -4.45 -9.73 -5.54 -2.04 6.74
Nucleolus -2.39 -3.88 -0.65 1.72 3.33 -0.87 -2.90 0.19 1.47 3.50 -1.99 -4.18 -0.12 2.23 4.41
Nucleus 1.60 2.15 3.37 3.10 2.45 -0.68 -1.70 2.68 2.04 3.36 1.25 1.72 3.89 3.88 4.14
Peroxisomes 3.92 -3.70 0.55 -3.38 4.03 2.32 -2.25 2.01 -0.28 4.34 4.40 -3.40 2.85 -1.68 5.85
Vacuole -5.74 -10.73 -11.80 -8.81 -0.15 0.45 -12.53 -20.43 -21.80 -5.90 -3.46 -16.35 -23.95 -22.19 -5.14
Vacuole Periphery 6.84 2.97 8.36 2.71 4.20 2.33 2.62 -2.05 -5.34 -5.55 6.62 5.42 3.83 -3.65 -2.33
Standard name
Human Ortholog
Description Endosomal protein of unknown function; contains a phox (PX) homology domain; binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport
Localization
Cell Percentages endosome (53%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ypt35

Ypt35


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypt35-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available