Standard name
Human Ortholog
Description Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.97 0.96 0.86 0.78 0.49 0.34 0.36 0.21 0.24 – 0.05 0.95 0.91 0.75 0.89 0.87 0.83 0.91 0.92 0.86 0.94 0.94 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.08 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.42 0 0.18 0.63 0.49 – 0.73 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0.11 0 0.18 0.2 0.14 0.15 – 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.24 0.2 0.24 0.55 0.43 0.24 0.27 – 0.45 0.07 0.16 0.31 0.2 0.25 0.24 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 1 1 0 2 2 4
Bud 0 1 0 0 0 1 0 2 0 0 – 0 1 0 0 0 1 2 1 1 0 1 6 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 1 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 0 0 1 0 0 0 – 0 5 1 0 6 6 3 0 0 0 0 0 0
Cytoplasm 258 391 43 36 35 27 33 72 13 21 – 1 709 787 694 432 440 463 320 371 58 598 799 794
Endoplasmic Reticulum 2 3 2 0 2 0 0 0 0 0 – 0 7 3 1 18 12 19 4 3 2 4 7 7
Endosome 2 0 0 1 0 0 8 11 0 2 – 0 5 5 22 8 4 14 4 3 1 4 3 19
Golgi 0 0 0 0 0 0 0 0 0 0 – 0 1 1 1 1 1 1 3 0 0 0 0 0
Mitochondria 2 3 0 0 0 23 4 36 40 42 – 16 3 2 0 6 1 8 3 4 3 6 7 11
Nucleus 5 5 0 0 5 1 17 41 9 13 – 2 5 4 24 7 2 2 3 5 0 0 1 2
Nuclear Periphery 6 1 0 0 0 1 0 0 1 0 – 0 0 0 0 0 0 0 1 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 1 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 1 1 0 2 5 10
Vac/Vac Membrane 7 5 2 10 9 13 53 86 15 23 – 10 51 135 287 96 127 133 3 7 0 7 6 7
Unique Cell Count 278 403 45 42 45 55 96 202 63 86 22 748 869 921 487 504 559 354 404 68 637 849 876
Labelled Cell Count 283 410 47 47 51 66 116 249 78 101 29 787 939 1030 576 594 646 354 404 68 637 849 876


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 5.5 6.0 7.1 8.2 5.2 6.8 6.4 5.3 5.6 – 4.7 6.9 7.0 7.0 7.0 7.6 6.8 5.7 5.9 5.9
Std Deviation (1e-4) 0.8 0.6 1.1 1.0 1.6 1.5 1.6 1.6 1.4 1.2 – 0.7 0.9 0.9 1.1 1.1 1.1 1.0 1.0 0.9 1.1
Intensity Change (Log2) – – – 0.23 0.44 -0.22 0.17 0.08 -0.17 -0.1 – -0.34 0.2 0.22 0.22 0.22 0.34 0.17 -0.07 -0.04 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm -1.6 -2.5 -5.0 -6.8 -7.3 -7.7 -7.7 – -7.4 -0.2 -1.1 -3.1 -1.4 -1.6 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 0 3.1 6.7 5.7 – 0 0 0 0 0 0 0
Nucleus 0 0 0 3.0 3.3 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vacuole 2.6 2.3 2.7 5.8 4.8 2.7 3.1 – 0 0 2.0 3.8 2.5 3.1 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8228 4.761 4.2776 3.9334 3.5693 4.1575 6.1489 7.25 7.0212 6.7163 6.8911 7.0838 8.3048 8.6675 8.8939 8.3723 8.3691 8.4965
Actin 0.0004 0.0002 0.0002 0.0003 0.0005 0.0002 0.0085 0.0002 0.0009 0.0024 0.0034 0.006 0.0168 0.0018 0.0005 0.0005 0.0006 0.0065
Bud 0.0002 0.0001 0.0002 0.0003 0.0001 0.0002 0.0006 0.0005 0.0008 0.0003 0.0005 0.0008 0.0018 0.0004 0.0002 0.0001 0.0002 0.0016
Bud Neck 0.0006 0.0005 0.0006 0.0015 0.0013 0.0028 0.0056 0.0008 0.0015 0.0036 0.0018 0.0036 0.0031 0.0006 0.0006 0.0006 0.0007 0.0016
Bud Periphery 0.0001 0 0 0.0001 0 0.0001 0.0007 0.0001 0.0005 0.0002 0.0005 0.0002 0.0005 0.0001 0 0 0 0.0004
Bud Site 0.0003 0.0008 0.0003 0.0003 0.0002 0.0001 0.0012 0.0018 0.0015 0.0004 0.0007 0.0007 0.0035 0.0013 0.0009 0.0003 0.0002 0.0002
Cell Periphery 0 0 0 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0.0001 0 0 0 0
Cytoplasm 0.7614 0.6773 0.7134 0.5393 0.7356 0.7196 0.6575 0.6173 0.6591 0.6329 0.7002 0.6064 0.8241 0.8017 0.8714 0.7806 0.8336 0.6889
Cytoplasmic Foci 0.0046 0.0047 0.0052 0.0025 0.0108 0.0052 0.0104 0.0021 0.005 0.0084 0.009 0.005 0.011 0.0176 0.0057 0.0038 0.0285 0.011
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.009 0.0042 0.0041 0.003 0.0046 0.004 0.0067 0.0033 0.0034 0.0067 0.0075 0.0027 0.0092 0.0032 0.0021 0.0033 0.0033 0.0043
Endosome 0.0089 0.0099 0.0063 0.0044 0.0099 0.0064 0.0143 0.0019 0.007 0.0092 0.0124 0.0042 0.0049 0.0019 0.0012 0.002 0.0061 0.0044
Golgi 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.005 0.0001 0.001 0.0012 0.0029 0.0006 0.0009 0.0002 0.0001 0.0002 0.0004 0.0006
Lipid Particles 0.0002 0 0.0001 0 0.0001 0 0.0032 0 0.001 0.0009 0.0033 0.0006 0.0006 0.0001 0 0.0001 0.0003 0.001
Mitochondria 0.0006 0.0004 0.0009 0.0006 0.0005 0.0006 0.0082 0.0004 0.0029 0.0015 0.021 0.0006 0.0008 0.0006 0.0002 0.0002 0.0003 0.0009
None 0.0261 0.0087 0.0176 0.0028 0.0085 0.0126 0.0196 0.0035 0.0103 0.0041 0.0077 0.0088 0.0214 0.0115 0.0198 0.0065 0.0053 0.0088
Nuclear Periphery 0.019 0.0119 0.0116 0.0148 0.012 0.0112 0.0159 0.0086 0.0091 0.0129 0.0154 0.0092 0.008 0.0084 0.005 0.0061 0.0062 0.0125
Nucleolus 0.0002 0.0004 0.0003 0.0004 0.0003 0.0003 0.0011 0.0003 0.0006 0.0007 0.0005 0.0004 0.0007 0.0002 0.0001 0.0001 0.0001 0.0004
Nucleus 0.1603 0.2717 0.23 0.4216 0.1927 0.2274 0.2224 0.3533 0.2841 0.2833 0.1899 0.3399 0.0754 0.1423 0.0875 0.1922 0.1051 0.2372
Peroxisomes 0.0001 0.0001 0.0002 0.0001 0.0002 0.0002 0.0036 0.0001 0.0007 0.0011 0.0028 0.0008 0.0005 0.0021 0.0001 0.0001 0.0006 0.0038
Punctate Nuclear 0.0039 0.0048 0.0065 0.0054 0.0205 0.0062 0.0107 0.0039 0.0071 0.0277 0.0169 0.008 0.0148 0.0047 0.0039 0.0023 0.0075 0.0143
Vacuole 0.0034 0.0034 0.0021 0.002 0.0014 0.0022 0.004 0.0014 0.0028 0.0017 0.0024 0.0014 0.0012 0.0011 0.0005 0.0008 0.0007 0.0012
Vacuole Periphery 0.0007 0.0006 0.0004 0.0005 0.0004 0.0004 0.0008 0.0002 0.0006 0.0004 0.0008 0.0003 0.0003 0.0002 0.0001 0.0001 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.5639 26.2334 24.6573 43.1502 34.2434 37.1508 30.5033 41.772 39.8714 47.6373
Translational Efficiency 4.7422 2.6686 2.5455 1.8037 2.2737 2.5032 2.611 1.5552 1.8742 1.7413

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1725 1295 2613 188 2036 2029 1109 309 3761 3324 3722 497

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 672.02 893.82 943.06 1029.24 721.48 856.86 1035.73 1062.36 698.79 871.26 970.67 1049.83
Standard Deviation 92.21 125.01 121.03 120.40 101.09 106.60 138.25 123.16 100.19 115.54 133.33 123.18
Intensity Change Log 2 — 0.411480 0.488845 0.615003 — 0.248100 0.521617 0.558241 — 0.329202 0.505906 0.585894

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000159 0.000419 0.000226 0.000518 0.000118 0.000242 0.000574 0.000252 0.000137 0.000311 0.000330 0.000352
Bud Neck 0.010847 0.006905 0.005432 0.002891 0.005770 0.011534 0.002821 0.003165 0.008099 0.009730 0.004654 0.003062
Bud Site 0.005818 0.010826 0.006718 0.004248 0.004521 0.008971 0.005420 0.004271 0.005116 0.009694 0.006331 0.004262
Cell Periphery 0.000141 0.000126 0.000068 0.000159 0.000114 0.000094 0.000151 0.000103 0.000127 0.000107 0.000092 0.000124
Cytoplasm 0.481285* 0.601946* 0.571650* 0.404968* 0.564996* 0.562201* 0.453344* 0.569579* 0.526601* 0.577686* 0.536400* 0.507312*
Cytoplasmic Foci 0.211792* 0.087326 0.005408 0.013185 0.121578 0.082770 0.007803 0.014977 0.162955 0.084545 0.006122 0.014299
Eisosomes 0.000101 0.000035 0.000008 0.000075 0.000059 0.000038 0.000027 0.000042 0.000078 0.000037 0.000014 0.000055
Endoplasmic Reticulum 0.002519 0.003689 0.001540 0.007754 0.001667 0.001198 0.004887 0.002919 0.002057 0.002168 0.002537 0.004748
Endosome 0.008292 0.006859 0.000528 0.004465 0.004002 0.005628 0.001858 0.002437 0.005969 0.006108 0.000925 0.003204
Golgi 0.003651 0.002246 0.000064 0.000131 0.000902 0.001579 0.000155 0.000096 0.002163 0.001839 0.000091 0.000109
Lipid Particles 0.010762 0.002213 0.000351 0.002324 0.004452 0.002593 0.001167 0.001402 0.007346 0.002445 0.000594 0.001751
Mitochondria 0.008446 0.002903 0.000746 0.000335 0.003013 0.004224 0.000982 0.000296 0.005505 0.003709 0.000817 0.000311
Mitotic Spindle 0.001340 0.007018 0.004517 0.015601 0.002956 0.006655 0.007057 0.010359 0.002214 0.006797 0.005274 0.012342
None 0.007250 0.005009 0.004723 0.007668 0.006042 0.007790 0.008117 0.007309 0.006596 0.006707 0.005734 0.007444
Nuclear Periphery 0.000746 0.001362 0.001267 0.002210 0.000741 0.000830 0.001690 0.001498 0.000743 0.001037 0.001393 0.001767
Nuclear Periphery Foci 0.000683 0.002587 0.000932 0.004032 0.000547 0.000520 0.004321 0.003458 0.000609 0.001325 0.001942 0.003675
Nucleolus 0.001932 0.001088 0.000146 0.000378 0.001385 0.001395 0.000264 0.000271 0.001636 0.001275 0.000181 0.000312
Nucleus 0.200050* 0.208970* 0.372169* 0.255485* 0.245981* 0.259797* 0.436792* 0.177614* 0.224915* 0.239995* 0.391424* 0.207070*
Peroxisomes 0.003958 0.002466 0.000217 0.000227 0.001606 0.003152 0.000755 0.000157 0.002685 0.002884 0.000377 0.000183
Vacuole 0.038564 0.044818 0.022733 0.273167* 0.028808 0.037743 0.061655 0.199640 0.033283 0.040499 0.034330 0.227453*
Vacuole Periphery 0.001665 0.001191 0.000555 0.000180 0.000743 0.001044 0.000159 0.000156 0.001166 0.001101 0.000437 0.000165

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.30 -1.97 -7.77 -4.60 -6.19 -2.22 -10.86 -6.45 -2.31 5.70 -4.91 -6.72 -9.80 -4.75 -3.28
Bud Neck 3.01 4.96 6.48 2.98 1.00 -4.58 3.60 0.76 4.29 -1.15 -2.11 4.59 3.99 5.03 0.19
Bud Site -3.66 -0.34 -1.17 2.95 -0.70 -3.59 -0.89 -1.07 2.94 0.06 -4.98 -1.03 -1.41 4.13 -0.09
Cell Periphery 0.92 4.56 -2.13 -3.92 -8.11 2.71 -5.70 0.95 -1.29 5.63 2.44 3.15 -0.77 -3.43 -4.14
Cytoplasm -11.44 -5.67 12.91 18.35 15.62 0.88 19.88 9.84 9.32 -1.11 -7.07 7.36 14.34 17.50 11.01
Cytoplasmic Foci 20.43 42.52 40.48 19.63 -3.39 8.60 33.57 30.02 21.88 -4.58 20.84 52.72 48.62 29.28 -6.22
Eisosomes 14.71 22.34 -0.20 -9.97 -14.27 6.56 8.01 0.30 -4.14 -4.70 15.10 26.06 0.88 -9.41 -15.21
Endoplasmic Reticulum -3.04 1.64 -7.56 -6.19 -8.20 1.94 -12.91 -5.01 -6.02 3.41 -0.87 -6.30 -8.59 -8.23 -6.40
Endosome 1.65 12.59 5.07 2.89 -11.73 -3.10 6.13 3.91 6.00 -2.47 -0.37 15.12 6.84 6.41 -12.47
Golgi 2.12 8.58 8.32 3.55 -6.30 -2.28 7.75 8.20 5.24 1.09 1.02 10.41 10.16 6.03 -4.33
Lipid Particles 10.54 13.05 9.81 -2.42 -13.52 4.23 10.09 8.81 2.78 -1.54 10.87 16.58 12.70 1.53 -12.34
Mitochondria 4.37 7.02 7.28 3.30 2.91 -1.23 4.02 5.29 4.63 2.86 2.35 8.19 8.89 5.65 4.56
Mitotic Spindle -3.42 -4.25 -2.60 -1.83 -2.06 -2.63 -2.87 -2.07 -1.16 -0.87 -4.28 -4.23 -3.34 -2.12 -2.32
None 2.33 2.99 3.51 1.72 1.69 -1.46 0.20 0.83 1.89 0.48 0.01 2.88 2.66 2.32 0.51
Nuclear Periphery -1.09 -10.52 -8.00 -3.69 -5.58 -0.84 -9.65 -6.67 -7.79 1.35 -1.36 -11.58 -10.49 -6.35 -4.88
Nuclear Periphery Foci -7.67 -3.56 -8.26 -4.67 -7.43 -0.09 -13.16 -8.55 -8.50 1.88 -6.84 -12.27 -11.79 -9.13 -6.55
Nucleolus 2.51 6.37 5.29 3.33 -3.58 -0.12 3.09 2.88 6.68 -1.75 1.39 6.31 5.49 7.26 -4.97
Nucleus -1.66 -29.90 -3.12 -2.38 8.22 -2.07 -22.86 1.75 2.82 16.18 -3.26 -36.35 -1.00 0.60 16.99
Peroxisomes 3.04 10.97 11.06 6.82 -0.75 -2.64 3.43 5.44 6.32 1.16 -0.59 10.39 11.55 8.47 0.76
Vacuole -4.03 0.14 -17.29 -16.66 -17.30 -5.03 -15.60 -17.92 -17.15 -12.18 -6.06 -11.96 -24.41 -23.48 -22.21
Vacuole Periphery 2.27 3.31 9.34 6.07 0.70 -1.37 7.53 7.25 4.47 -0.48 0.54 3.26 11.73 6.76 0.75
Standard name
Human Ortholog
Description Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (54%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Lsm12

Lsm12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lsm12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available