ORF
Human Ortholog
Description Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study; YHR131C has a paralog, YNL144C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 0.99 0.99 0.97 0.98 0.96 0.92 – 0.87 0.89 0.99 0.99 1.0 0.98 0.98 0.98 0.92 0.93 0.88 0.86 0.86 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0 0.17 – 0.3 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 5 6 1 4 14
Bud 0 3 0 0 0 0 2 0 6 – 4 4 0 1 0 0 0 0 0 6 8 2 7 16
Bud Neck 0 0 3 3 9 2 1 9 5 – 1 2 0 0 0 1 0 0 0 1 5 2 2 11
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 16 12 5 10 5 2 4 2 – 3 7 3 13 4 7 12 11 0 1 6 1 7 18
Cytoplasm 162 715 650 369 580 479 623 598 585 – 367 458 309 616 619 238 233 160 150 668 553 147 423 435
Endoplasmic Reticulum 1 3 1 0 0 0 1 0 0 – 0 0 1 3 2 12 8 9 0 2 3 2 7 12
Endosome 0 1 3 0 0 1 0 1 2 – 2 0 0 0 0 0 2 2 4 5 2 1 4 3
Golgi 0 2 0 0 0 0 0 0 0 – 0 0 0 0 0 1 2 0 0 4 2 0 1 0
Mitochondria 0 1 1 4 2 20 50 22 108 – 126 98 2 1 1 4 3 1 0 5 11 6 9 11
Nucleus 1 2 1 1 4 4 5 5 6 – 2 5 0 2 1 1 1 2 1 0 2 0 0 0
Nuclear Periphery 0 1 0 0 0 1 1 0 0 – 0 0 0 2 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 0 1 1 6 3 – 5 2 0 1 0 0 1 0 1 4 7 0 3 4
Vac/Vac Membrane 0 3 2 0 0 2 2 3 7 – 6 3 1 1 0 1 1 3 0 1 1 1 12 11
Unique Cell Count 163 730 658 371 588 492 638 620 634 423 517 312 623 621 243 238 164 163 720 628 171 492 551
Labelled Cell Count 164 747 675 382 606 515 688 648 724 517 580 317 640 627 265 263 188 163 720 628 171 492 551


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.7 5.4 4.8 5.3 4.3 4.4 4.7 3.8 – 3.7 4.0 5.7 5.7 5.7 6.9 7.2 7.0 5.0 5.1 5.5
Std Deviation (1e-4) 0.7 0.8 0.8 0.9 0.7 1.1 1.0 0.7 1.0 – 0.9 0.6 1.4 1.1 1.0 1.1 1.4 1.6 1.2 1.4 1.6
Intensity Change (Log2) – – – -0.16 -0.04 -0.32 -0.3 -0.2 -0.51 – -0.53 -0.44 0.07 0.06 0.08 0.36 0.42 0.38 -0.11 -0.08 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 1.9 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.2 0 0 0 0 – 0 0 0 0.3 -1.9 1.0 2.6 0
Cytoplasm 1.1 -0.2 -1.8 -1.5 -2.8 -5.7 – -8.2 -7.5 0.3 0.2 1.8 -0.9 -1.0 -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6846 2.2685 1.8426 1.8095 1.728 1.885 1.4638 0.961 1.2834 0.8804 0.9468 1.0969 0.3324 0.8696 0.6655 0.6346 0.6269 0.8374
Actin 0.0405 0.0001 0.0167 0.0323 0.055 0.0018 0.0459 0.0035 0.0282 0.0418 0.0055 0.014 0.0002 0.0005 0.0029 0.0005 0.0005 0.0026
Bud 0.0014 0 0.0024 0.0015 0.0011 0.0001 0.0023 0.002 0.0075 0.0009 0.0037 0.0017 0.0001 0.0009 0.0018 0.0007 0.0004 0.005
Bud Neck 0.0018 0 0.0007 0.0007 0.0004 0.0008 0.0069 0.0005 0.0018 0.0049 0.0012 0.0028 0.0003 0.0003 0.0004 0.0003 0.0126 0.0058
Bud Periphery 0.0035 0.0001 0.0049 0.0028 0.0058 0.0004 0.005 0.0023 0.0113 0.0021 0.0143 0.0053 0.0001 0.0009 0.0013 0.001 0.0005 0.0037
Bud Site 0.0049 0.0003 0.0073 0.0146 0.0008 0 0.0127 0.0324 0.0368 0.0032 0.0081 0.0018 0.0007 0.0133 0.0049 0.0002 0.0027 0.0007
Cell Periphery 0.0005 0.0006 0.0004 0.0004 0.0005 0.0002 0.0008 0.0011 0.0019 0.0006 0.0012 0.0003 0.0001 0.0004 0.0003 0.0002 0.0001 0.0002
Cytoplasm 0.1409 0.2404 0.2733 0.1217 0.196 0.3129 0.2445 0.3706 0.2738 0.3955 0.2998 0.3844 0.1858 0.3119 0.2122 0.3195 0.3982 0.3048
Cytoplasmic Foci 0.0181 0.0142 0.0077 0.0049 0.0346 0.0052 0.0296 0.0088 0.0247 0.0159 0.0913 0.0097 0.0113 0.008 0.0072 0.0069 0.0102 0.0036
Eisosomes 0.0011 0.0001 0.0008 0.0006 0.0009 0.0001 0.0004 0.0002 0.0005 0.0005 0.0002 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0027 0.001 0.0023 0.0014 0.0016 0.0005 0.0066 0.0046 0.0057 0.0025 0.0017 0.0035 0.001 0.0022 0.0022 0.003 0.0008 0.0012
Endosome 0.0077 0.0008 0.0059 0.0053 0.0097 0.001 0.0276 0.0061 0.0177 0.0048 0.0098 0.0111 0.001 0.0024 0.0022 0.0049 0.0027 0.001
Golgi 0.0037 0.0001 0.0012 0.0042 0.0103 0.0001 0.0105 0.0008 0.011 0.0026 0.0027 0.0074 0.0001 0.0002 0.0002 0.0003 0.0002 0.0001
Lipid Particles 0.0072 0.001 0.0021 0.0123 0.0311 0.0001 0.0101 0.0008 0.008 0.0041 0.0045 0.0038 0.0004 0.0003 0.0008 0.0003 0.0001 0.0001
Mitochondria 0.0051 0.0001 0.0012 0.0082 0.002 0.0001 0.0109 0.0009 0.0144 0.0019 0.0127 0.0033 0.0001 0.0002 0.0005 0.0005 0.0003 0.0002
None 0.7275 0.7398 0.6529 0.7351 0.5774 0.6749 0.5307 0.5508 0.5225 0.4999 0.4965 0.5395 0.7957 0.6541 0.7562 0.6548 0.5642 0.6673
Nuclear Periphery 0.0036 0.0002 0.0064 0.004 0.0008 0.0001 0.0116 0.0021 0.0057 0.0011 0.0009 0.002 0.0003 0.0004 0.0008 0.0011 0.0003 0.0003
Nucleolus 0.0005 0 0.0008 0.0061 0.0002 0.0001 0.001 0.0005 0.0009 0.0003 0.0008 0.0004 0.0001 0.0002 0.0005 0.0002 0.0008 0.0001
Nucleus 0.0103 0.0002 0.0048 0.0111 0.0007 0.0003 0.0188 0.0033 0.0042 0.0019 0.0017 0.0014 0.0004 0.0015 0.0019 0.0022 0.0023 0.0016
Peroxisomes 0.0134 0.0001 0.0026 0.0249 0.0664 0.0004 0.0055 0.0026 0.0116 0.012 0.0195 0.0016 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001
Punctate Nuclear 0.0044 0.0005 0.0038 0.0058 0.0021 0.0002 0.0109 0.0018 0.0072 0.0016 0.0034 0.0037 0.0014 0.001 0.0022 0.001 0.0016 0.0006
Vacuole 0.0008 0.0004 0.0013 0.0013 0.0022 0.0007 0.0057 0.0038 0.0032 0.0014 0.0172 0.0018 0.0004 0.0011 0.0009 0.0017 0.0013 0.0008
Vacuole Periphery 0.0004 0 0.0004 0.0006 0.0004 0 0.002 0.0003 0.0014 0.0003 0.0034 0.0006 0 0.0001 0.0001 0.0003 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.644 8.1136 13.7614 13.1049 10.9271 11.231 16.7002 15.9515 15.1392 13.9705
Translational Efficiency 0.4482 0.4671 0.3412 0.4589 0.2799 0.395 0.2853 0.2543 0.3509 0.2742

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1992 1433 258 1260 1535 819 2287 2872 3527 2252 2545 4132

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 699.87 733.05 913.97 833.47 707.68 696.16 844.45 885.73 703.27 719.63 851.50 869.79
Standard Deviation 79.54 103.09 95.96 126.54 88.22 91.90 106.04 106.69 83.52 100.74 107.13 115.65
Intensity Change Log 2 — 0.066825 0.385060 0.252043 — -0.023678 0.254915 0.323770 — 0.022032 0.321094 0.288551

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000217 0.000845 0.000539 0.001609 0.000185 0.000490 0.000143 0.000141 0.000203 0.000716 0.000183 0.000589
Bud Neck 0.015847 0.024730 0.003363 0.014513 0.011587 0.040087 0.006352 0.024051 0.013993 0.030315 0.006049 0.021142
Bud Site 0.009733 0.016205 0.004431 0.061560 0.005787 0.025498 0.013598 0.035250 0.008015 0.019585 0.012669 0.043273
Cell Periphery 0.000218 0.000173 0.000373 0.000222 0.000130 0.000241 0.000130 0.000268 0.000180 0.000198 0.000155 0.000254
Cytoplasm 0.616508* 0.533135* 0.265526* 0.420659* 0.606069* 0.463959* 0.604781* 0.571583* 0.611965* 0.507977* 0.570389* 0.525560*
Cytoplasmic Foci 0.132789 0.189315* 0.038924 0.011598 0.166345 0.230511* 0.011408 0.005506 0.147393 0.204297* 0.014197 0.007364
Eisosomes 0.000261 0.000176 0.000176 0.000040 0.000153 0.000257 0.000030 0.000046 0.000214 0.000205 0.000045 0.000044
Endoplasmic Reticulum 0.000444 0.000684 0.007918 0.002374 0.000466 0.000529 0.001139 0.000496 0.000454 0.000627 0.001826 0.001069
Endosome 0.006922 0.014554 0.008729 0.005732 0.008214 0.024013 0.001738 0.002613 0.007484 0.017994 0.002446 0.003564
Golgi 0.001892 0.002555 0.000141 0.003215 0.001701 0.005167 0.000334 0.000392 0.001809 0.003505 0.000314 0.001253
Lipid Particles 0.003118 0.004271 0.006018 0.001043 0.003196 0.005440 0.000655 0.000380 0.003152 0.004696 0.001199 0.000582
Mitochondria 0.001542 0.005302 0.000569 0.002463 0.001461 0.011124 0.000756 0.004018 0.001506 0.007419 0.000737 0.003544
Mitotic Spindle 0.001821 0.002413 0.000649 0.073852 0.001777 0.010789 0.016451 0.025835 0.001802 0.005459 0.014849 0.040477
None 0.003974 0.002256 0.004699 0.010174 0.002550 0.002169 0.004079 0.006079 0.003354 0.002225 0.004142 0.007328
Nuclear Periphery 0.000142 0.000246 0.002642 0.001428 0.000201 0.000602 0.001071 0.000909 0.000168 0.000375 0.001230 0.001067
Nuclear Periphery Foci 0.000398 0.000546 0.003316 0.001714 0.000397 0.000330 0.000629 0.000150 0.000397 0.000468 0.000901 0.000627
Nucleolus 0.000878 0.001262 0.001101 0.000733 0.000598 0.000863 0.000289 0.000840 0.000756 0.001117 0.000371 0.000808
Nucleus 0.115000 0.113703 0.458908* 0.303027* 0.115719 0.093226 0.260398* 0.274310* 0.115313 0.106256 0.280522* 0.283067*
Peroxisomes 0.002451 0.007649 0.000695 0.002691 0.002811 0.011639 0.000150 0.000529 0.002607 0.009100 0.000205 0.001188
Vacuole 0.085405 0.078686 0.190973 0.080095 0.069916 0.071462 0.075583 0.045600 0.078664 0.076059 0.087281 0.056119
Vacuole Periphery 0.000442 0.001293 0.000312 0.001258 0.000738 0.001605 0.000286 0.001004 0.000570 0.001407 0.000289 0.001081

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.05 -7.16 -4.41 -0.39 -0.30 -4.69 0.47 1.31 5.73 1.79 -2.63 -1.47 -3.38 1.58 -2.86
Bud Neck -3.91 9.37 3.40 6.29 -5.00 -8.48 5.46 -5.19 6.22 -11.00 -8.75 9.58 -2.22 7.29 -11.39
Bud Site -3.41 1.58 -5.52 -3.18 -6.18 -7.04 -2.88 -9.11 1.22 -6.07 -7.46 -1.06 -9.69 -1.79 -7.79
Cell Periphery 2.79 -6.28 4.10 1.09 8.20 -3.34 1.32 -1.70 2.20 -2.38 -1.16 4.24 1.54 2.05 -0.88
Cytoplasm 9.91 29.24 33.62 20.55 -7.28 12.07 12.52 13.69 -2.61 0.39 14.96 20.86 29.29 9.19 3.77
Cytoplasmic Foci -9.24 22.64 39.06 34.96 6.15 -7.50 35.29 36.71 30.92 9.28 -11.29 50.34 53.74 46.74 10.10
Eisosomes 3.23 3.91 18.13 4.96 12.21 -3.42 11.58 11.01 7.58 0.88 0.49 19.89 20.79 8.57 4.16
Endoplasmic Reticulum -2.90 -12.41 -17.17 -15.66 8.69 -0.52 -15.61 -6.98 -2.93 12.04 -2.61 -18.09 -17.38 -12.93 8.24
Endosome -6.82 -3.03 9.14 10.79 8.11 -7.07 9.44 8.92 10.59 -0.10 -9.61 13.14 13.41 15.42 1.70
Golgi -1.39 4.65 1.87 3.56 -1.89 -3.99 6.34 6.92 5.87 -0.22 -4.04 6.47 5.35 8.39 -1.48
Lipid Particles -2.43 -6.06 7.05 6.64 10.15 -3.79 8.88 10.58 9.00 6.76 -4.14 8.70 13.41 11.40 8.61
Mitochondria -3.34 2.91 -0.49 3.31 -7.53 -4.88 2.36 -2.60 4.18 -5.16 -5.85 3.46 -2.61 4.83 -6.21
Mitotic Spindle -0.64 -0.43 -9.82 -9.48 -9.14 -3.53 -5.69 -9.48 -3.04 -3.10 -3.18 -5.94 -13.74 -10.22 -6.46
None 4.27 0.86 -6.34 -8.98 -5.17 0.58 -3.85 -11.45 -8.61 -8.87 3.44 -1.65 -11.90 -14.37 -10.48
Nuclear Periphery -5.20 -18.89 -23.26 -22.21 1.12 -1.18 -25.59 -24.37 -4.70 -2.22 -1.75 -31.56 -34.02 -15.19 -4.09
Nuclear Periphery Foci -1.78 -8.61 -7.18 -5.26 5.72 0.85 -5.32 5.22 1.54 7.86 -1.27 -9.08 -3.97 -1.55 5.55
Nucleolus -1.07 -0.03 1.14 1.72 1.46 -2.13 3.35 -2.36 -0.06 -6.37 -1.61 4.38 -0.53 1.41 -6.47
Nucleus -0.02 -17.36 -31.99 -30.59 1.79 4.11 -33.62 -40.52 -38.92 -3.94 2.32 -39.63 -52.95 -49.98 -4.76
Peroxisomes -6.68 5.43 3.29 8.65 -0.67 -7.55 6.70 6.24 10.44 -2.95 -9.93 9.59 7.72 14.26 -2.64
Vacuole 0.98 -11.74 -12.51 -12.62 5.66 -0.03 -12.91 -1.83 -1.45 11.77 0.43 -15.61 -8.68 -8.23 8.11
Vacuole Periphery -4.23 3.23 0.02 3.88 -1.31 -2.38 1.88 0.62 4.13 -2.57 -4.22 2.76 0.40 5.07 -3.00
ORF
Human Ortholog
Description Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study; YHR131C has a paralog, YNL144C, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YHR131C

YHR131C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhr131c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available