Standard name
Human Ortholog
Description 6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.97 0.96 0.92 0.88 0.81 0.73 0.79 0.8 0.74 0.72 0.99 1.0 0.99 0.97 0.97 0.98 0.96 0.94 0.96 0.98 0.96 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.08 0.06 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.09 0.1 0.14 0.22 0.11 0.11 0.16 0.17 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 1 0 1 1 4 3 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1 6 2 2 1 0 0 0 0 0 0 0 1 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 6 3 6 10 9 13 9 15 6 16 2 0 0 2 2 2 0 0 0 0 0 0
Cytoplasm 141 160 496 282 423 502 489 483 396 298 287 400 220 266 317 179 146 179 140 159 481 166 243 188
Endoplasmic Reticulum 0 2 1 1 4 10 19 21 19 13 12 15 1 0 0 9 10 8 0 0 3 0 0 1
Endosome 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
Golgi 0 0 1 0 0 1 1 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Mitochondria 1 1 0 0 1 3 7 4 38 24 35 43 0 0 0 1 2 0 1 1 1 0 0 0
Nucleus 0 0 5 2 2 3 12 25 10 10 7 20 2 0 0 0 1 1 0 2 2 0 3 3
Nuclear Periphery 0 0 0 0 0 0 0 1 1 2 0 1 0 0 0 0 0 0 1 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 11 16 40 60 84 148 57 40 63 94 3 0 5 2 3 9 0 2 0 0 2 0
Unique Cell Count 142 165 509 295 462 572 601 665 501 374 390 556 223 266 319 184 151 183 147 169 500 170 254 197
Labelled Cell Count 143 169 520 305 476 590 622 699 537 408 415 593 228 266 323 194 164 200 147 169 500 170 254 197


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.3 12.5 16.0 14.9 14.0 12.1 11.4 11.3 10.0 10.1 9.8 9.5 16.4 15.9 15.3 23.2 27.3 31.2 15.6 15.1 14.1
Std Deviation (1e-4) 3.6 2.8 2.8 2.8 2.3 2.2 2.0 2.1 2.1 2.2 2.1 2.1 3.5 3.3 3.1 7.7 9.1 10.6 3.1 2.9 3.0
Intensity Change (Log2) – – – -0.1 -0.19 -0.4 -0.48 -0.51 -0.68 -0.66 -0.71 -0.75 0.04 -0.01 -0.07 0.54 0.77 0.96 -0.04 -0.08 -0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.2 0.8 0.5 1.0 0.8 2.7 0.5 1.9 0 0 0 0 0 0
Cytoplasm -1.4 -4.1 -6.0 -8.4 -11.3 -9.1 -8.7 -10.6 -11.4 1.0 2.6 2.0 -0.1 -0.5 0.3
Endoplasmic Reticulum 0 0 2.5 3.7 3.7 4.1 3.9 3.6 3.4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 6.3 5.8 6.9 6.4 0 0 0 0 0 0
Nucleus 0 0 0 1.4 3.0 1.3 1.9 1.1 2.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.5 4.5 5.5 7.0 10.0 5.8 5.4 7.6 8.1 0 0 -0.6 0 0 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 19.3509 22.7225 20.9771 19.1235 19.1347 21.6322 13.6233 22.6208 20.7677 18.396 17.3834 19.8429 15.4295 21.3478 17.7851 17.2722 17.8704 18.8542
Actin 0.0223 0.0002 0.0117 0.0072 0.0481 0.0002 0.0372 0.0001 0.0005 0.0029 0.0055 0.0071 0.0179 0.0002 0.0112 0.0017 0.0011 0.0026
Bud 0.0004 0 0.0002 0.0002 0.0031 0.0001 0.001 0 0.0001 0.0001 0.0001 0.0001 0.0003 0 0.0008 0.0001 0.0006 0.0004
Bud Neck 0.0004 0.0002 0.0009 0.0005 0.008 0.001 0.0028 0.0001 0.0002 0.0003 0.0005 0.0006 0.0004 0.0001 0.0003 0.0003 0.0004 0.0009
Bud Periphery 0.0005 0 0.0001 0.0002 0.006 0 0.0009 0 0 0.0001 0.0001 0.0001 0.0001 0 0.0004 0.0001 0.0004 0.0004
Bud Site 0.0031 0.0001 0.0011 0.0003 0.0054 0 0.0075 0 0.0001 0.0001 0.0009 0.0002 0.0006 0 0.0004 0.0001 0.0007 0.0001
Cell Periphery 0.0001 0 0 0 0.0008 0 0.0002 0 0 0 0.0002 0 0 0 0.0001 0 0.0001 0.0001
Cytoplasm 0.8944 0.9912 0.9411 0.9073 0.8027 0.9759 0.609 0.9961 0.9726 0.8681 0.8821 0.9525 0.902 0.9955 0.8985 0.8818 0.9757 0.9626
Cytoplasmic Foci 0.0073 0.0003 0.0045 0.026 0.0176 0.0006 0.074 0.0003 0.009 0.033 0.0461 0.0052 0.0208 0.0002 0.0085 0.0031 0.0023 0.0014
Eisosomes 0.0003 0 0.0001 0.0002 0.0002 0 0.0002 0 0 0 0.0001 0 0 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0035 0.0004 0.0021 0.0005 0.0016 0.0018 0.0081 0.0001 0.0003 0.0022 0.0071 0.0004 0.0036 0.0001 0.0078 0.0005 0.0044 0.0066
Endosome 0.0114 0.0001 0.0082 0.0026 0.0178 0.0004 0.06 0 0.0069 0.0336 0.0083 0.0024 0.0122 0 0.0234 0.0512 0.0026 0.0093
Golgi 0.0029 0 0.0052 0.0018 0.0053 0 0.0188 0 0.0023 0.0107 0.0026 0.0036 0.0047 0 0.0052 0.0064 0.0004 0.0025
Lipid Particles 0.0042 0 0.0007 0.0015 0.0031 0 0.0224 0 0.0012 0.0343 0.025 0.004 0.0027 0 0.0021 0.0089 0.0001 0
Mitochondria 0.0054 0 0.0024 0.0332 0.0074 0.0001 0.0063 0 0.0001 0.0011 0.0016 0.0012 0.0014 0 0.0161 0.0354 0.0009 0.0039
None 0.0115 0.0003 0.0029 0.0004 0.002 0.0003 0.0105 0.0004 0.0004 0.0013 0.0088 0.0004 0.0027 0.0003 0.0035 0.0003 0.0015 0.0004
Nuclear Periphery 0.0051 0.0009 0.0019 0.0011 0.0045 0.0029 0.0668 0.0003 0.0006 0.0009 0.0011 0.001 0.0044 0.0003 0.0059 0.0008 0.0016 0.0013
Nucleolus 0.0004 0 0.0015 0 0.0009 0 0.0007 0 0 0 0.0002 0 0.0001 0 0.0001 0.0001 0 0
Nucleus 0.0206 0.0061 0.0098 0.0105 0.0173 0.0158 0.0189 0.0023 0.0048 0.006 0.004 0.015 0.0144 0.003 0.0089 0.0059 0.0056 0.0064
Peroxisomes 0.0018 0 0.0027 0.0041 0.0036 0 0.0242 0 0.0006 0.0034 0.002 0.0056 0.0016 0 0.0012 0.0009 0.0001 0
Punctate Nuclear 0.0032 0.0001 0.0019 0.0019 0.0276 0.0002 0.0248 0.0001 0.0002 0.0011 0.0023 0.0003 0.009 0.0001 0.0037 0.0002 0.0003 0.0002
Vacuole 0.001 0.0001 0.0007 0.0002 0.0152 0.0006 0.0022 0 0.0001 0.0005 0.0009 0.0002 0.0006 0 0.001 0.0009 0.0012 0.0005
Vacuole Periphery 0.0004 0 0.0002 0.0001 0.0018 0.0001 0.0037 0 0 0.0003 0.0003 0.0001 0.0003 0 0.0007 0.0012 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 119.9033 98.6376 139.5554 107.5903 96.3279 110.2794 121.6735 103.2127 128.1317 90.7313
Translational Efficiency 0.8133 0.8878 0.6499 0.937 0.7805 0.7747 0.8817 0.9891 0.8558 0.8278

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description 6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Sol3

Sol3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sol3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available