Standard name | |
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Human Ortholog | |
Description | Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 4 | 1 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 4 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 4 | 5 | 4 | 4 | 8 | 13 | 12 | 10 | 10 | 7 | 5 | 5 | 6 | 3 | 16 | 25 | 16 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 723 | 240 | 303 | 121 | 258 | 208 | 278 | 261 | 161 | 213 | 145 | 173 | 141 | 135 | 135 | 485 | 324 | 270 | 692 | 228 | 293 | 59 | 91 | 97 |
Endoplasmic Reticulum | 4 | 3 | 6 | 2 | 2 | 8 | 13 | 16 | 7 | 11 | 5 | 3 | 2 | 2 | 1 | 13 | 5 | 17 | 6 | 3 | 18 | 1 | 3 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 1 | 2 | 3 | 8 | 10 | 16 | 20 | 59 | 23 | 58 | 0 | 0 | 0 | 5 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 3 | 1 | 2 | 0 | 2 | 0 | 1 | 1 | 2 | 6 | 2 | 1 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 1 | 4 | 0 | 8 | 10 | 14 | 10 | 1 | 17 | 2 | 9 | 0 | 0 | 0 | 5 | 4 | 0 | 2 | 3 | 0 | 0 | 2 | 2 |
Unique Cell Count | 730 | 243 | 313 | 125 | 271 | 238 | 314 | 303 | 189 | 285 | 177 | 232 | 143 | 137 | 137 | 500 | 334 | 273 | 712 | 239 | 323 | 68 | 102 | 107 |
Labelled Cell Count | 733 | 249 | 323 | 130 | 277 | 250 | 331 | 321 | 206 | 322 | 189 | 253 | 148 | 144 | 140 | 529 | 365 | 306 | 712 | 239 | 323 | 68 | 102 | 107 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 19.6 | 24.6 | 21.1 | 20.0 | 16.5 | 14.2 | 12.9 | 12.0 | 11.4 | 10.3 | 10.8 | 10.5 | 19.7 | 18.9 | 18.3 | 25.9 | 32.2 | 31.4 | 19.2 | 19.5 | 20.0 |
Std Deviation (1e-4) | 3.2 | 5.2 | 3.5 | 3.5 | 2.6 | 2.5 | 2.6 | 2.3 | 2.5 | 2.0 | 2.3 | 1.9 | 3.2 | 3.1 | 2.7 | 7.5 | 8.0 | 8.2 | 5.2 | 4.9 | 5.1 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.36 | -0.57 | -0.71 | -0.81 | -0.88 | -1.04 | -0.97 | -1.0 | -0.1 | -0.16 | -0.21 | 0.29 | 0.61 | 0.57 | -0.14 | -0.11 | -0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 1.4 | 1.9 | 1.8 | 2.4 | 1.5 | 0 | 0 | 0 | 0 | 0 | 1.4 | 3.6 | 2.8 |
Cytoplasm | 0 | -1.0 | -4.2 | -4.0 | -4.8 | -4.8 | -7.8 | -5.7 | -7.7 | 1.1 | 1.1 | 1.1 | 0.2 | 0.1 | 1.7 |
Endoplasmic Reticulum | 0 | 0 | 1.1 | 1.6 | 2.2 | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0.6 | -0.4 | 2.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2.7 | 3.8 | 5.6 | 8.3 | 6.2 | 9.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.4 | 2.2 | 2.4 | 1.7 | 0 | 3.1 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.3887 | 19.386 | 17.1037 | 15.5235 | 16.5239 | 17.447 | 16.9093 | 23.1403 | 21.504 | 22.2696 | 19.5923 | 20.8168 | 17.6224 | 23.9029 | 21.5993 | 19.9351 | 20.0921 | 22.4025 |
Actin | 0.0305 | 0.0004 | 0.0138 | 0.0005 | 0.0002 | 0.0007 | 0.0182 | 0.0003 | 0.0069 | 0.0001 | 0.0001 | 0.0024 | 0.0051 | 0.0036 | 0.0215 | 0.043 | 0.014 | 0.0003 |
Bud | 0.0008 | 0.0002 | 0.0009 | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0 | 0.001 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0005 | 0.0004 | 0.0004 | 0.0002 | 0.0001 |
Bud Neck | 0.0044 | 0.0002 | 0.0006 | 0.0004 | 0.0007 | 0.0011 | 0.0009 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0006 | 0.0006 | 0.0002 | 0.0005 | 0.0014 | 0.0004 | 0.0005 |
Bud Periphery | 0.0007 | 0 | 0.001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0004 | 0 | 0.0001 | 0.0009 | 0.0003 | 0.0003 | 0.0006 | 0.0004 | 0.0002 | 0 |
Bud Site | 0.0008 | 0.0005 | 0.006 | 0.0002 | 0.0001 | 0.0001 | 0.005 | 0.0001 | 0.0008 | 0 | 0.0004 | 0.0002 | 0.0061 | 0.0051 | 0.0041 | 0.0017 | 0.0001 | 0 |
Cell Periphery | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0 | 0 |
Cytoplasm | 0.8155 | 0.9651 | 0.9032 | 0.9393 | 0.9641 | 0.8876 | 0.7824 | 0.9947 | 0.9404 | 0.9719 | 0.9161 | 0.9336 | 0.7958 | 0.9786 | 0.9174 | 0.8485 | 0.8939 | 0.9646 |
Cytoplasmic Foci | 0.0097 | 0.0015 | 0.0084 | 0.0035 | 0.0029 | 0.003 | 0.0347 | 0.0008 | 0.0057 | 0.0018 | 0.0161 | 0.0038 | 0.0451 | 0.0017 | 0.0081 | 0.0064 | 0.0106 | 0.0023 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0406 | 0.0259 | 0.0122 | 0.011 | 0.0032 | 0.0551 | 0.0115 | 0.002 | 0.0044 | 0.0148 | 0.0053 | 0.0047 | 0.0106 | 0.0033 | 0.0174 | 0.003 | 0.0039 | 0.0101 |
Endosome | 0.0331 | 0.0016 | 0.0118 | 0.0156 | 0.0106 | 0.0089 | 0.0565 | 0.0005 | 0.0157 | 0.0025 | 0.0338 | 0.0163 | 0.0156 | 0.0008 | 0.007 | 0.0265 | 0.0272 | 0.0038 |
Golgi | 0.0111 | 0.0002 | 0.0085 | 0.001 | 0.0004 | 0.0015 | 0.0257 | 0.0001 | 0.0062 | 0.0001 | 0.0009 | 0.0021 | 0.0042 | 0.0003 | 0.0026 | 0.004 | 0.0027 | 0.0006 |
Lipid Particles | 0.0096 | 0.0001 | 0.0031 | 0.0001 | 0 | 0.0002 | 0.0117 | 0 | 0.0016 | 0 | 0.0004 | 0.0004 | 0.0149 | 0.0006 | 0.0058 | 0.0016 | 0.004 | 0 |
Mitochondria | 0.0035 | 0.0001 | 0.0043 | 0.0005 | 0.0003 | 0.0308 | 0.0067 | 0 | 0.0072 | 0.0001 | 0.0008 | 0.0206 | 0.002 | 0.0001 | 0.0033 | 0.0496 | 0.0013 | 0.0099 |
None | 0.0027 | 0.0002 | 0.0013 | 0.0003 | 0.0002 | 0.0003 | 0.0103 | 0.0002 | 0.0011 | 0.0002 | 0.002 | 0.0004 | 0.0054 | 0.0008 | 0.0019 | 0.0006 | 0.0032 | 0.0003 |
Nuclear Periphery | 0.0121 | 0.0015 | 0.0081 | 0.0048 | 0.0016 | 0.0026 | 0.0083 | 0.0004 | 0.0017 | 0.0034 | 0.0028 | 0.0033 | 0.0059 | 0.0009 | 0.0021 | 0.0021 | 0.0172 | 0.0044 |
Nucleolus | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0.0011 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0 |
Nucleus | 0.0082 | 0.001 | 0.0051 | 0.0025 | 0.0019 | 0.0017 | 0.0069 | 0.0005 | 0.0016 | 0.0021 | 0.0022 | 0.0018 | 0.0073 | 0.0011 | 0.0013 | 0.0022 | 0.0026 | 0.0019 |
Peroxisomes | 0.0016 | 0 | 0.0039 | 0 | 0 | 0.0001 | 0.0044 | 0 | 0.001 | 0 | 0.0002 | 0.0002 | 0.0407 | 0.0001 | 0.0013 | 0.0034 | 0.0016 | 0 |
Punctate Nuclear | 0.0014 | 0 | 0.0009 | 0.0001 | 0 | 0.0001 | 0.0092 | 0 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0362 | 0.0003 | 0.0015 | 0.0006 | 0.0127 | 0.0001 |
Vacuole | 0.011 | 0.0011 | 0.0045 | 0.0163 | 0.0106 | 0.0044 | 0.0042 | 0.0002 | 0.0022 | 0.0022 | 0.0148 | 0.0052 | 0.002 | 0.0007 | 0.0017 | 0.0029 | 0.0021 | 0.0007 |
Vacuole Periphery | 0.0018 | 0.0002 | 0.002 | 0.0031 | 0.0027 | 0.0012 | 0.0013 | 0 | 0.0009 | 0.0003 | 0.0026 | 0.0026 | 0.0005 | 0.0001 | 0.0009 | 0.001 | 0.0014 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 110.9074 | 98.8277 | 99.1895 | 70.1304 | 85.4508 | 63.7453 | 78.6265 | 61.8221 | 51.0012 | 72.8289 |
Translational Efficiency | 0.9008 | 1.0668 | 0.8432 | 0.7913 | 0.8194 | 0.9469 | 0.8812 | 0.7828 | 0.8503 | 0.8587 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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