Standard name
Human Ortholog
Description Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.1 0 0.05 0 0 0 0 0 0.06 0.07 0.12 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.08 0 0 0 0.05 0 0 0.07 0 0.09 0.06 0 0 0 0.11 0.1 0.09 0 0 0 0 0 0
Nucleus 0.14 0.13 0.16 0.06 0.05 0.06 0 0.06 0 0.05 0.05 0 0.08 0 0.06 0.12 0.12 0.17 0.07 0.09 0.11 0.06 0.06 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.94 0.92 0.9 0.88 0.85 0.89 0.86 0.86 0.83 0.84 0.88 0.89 0.89 0.87 0.82 0.85 0.86 0.85 0.84 0.84 0.78 0.82 0.8 0.77
Peroxisomes 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.13 0.13 0.18 0.32 0.33 0.36 0.37 0.44 0.34 0.42 0.09 0.15 0.15 0 0 0 0.06 0 0.05 0.05 0.07 0.11
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 0 0 0 6 3 2 0 0 0 0 0 1 1 2 1 1 0 0
Bud Neck 4 7 2 6 3 9 11 10 11 5 10 10 8 5 0 1 1 0 0 1 1 0 0 1
Bud Site 0 0 0 0 0 3 7 5 10 6 13 6 0 0 0 0 0 2 – – – – – –
Cell Periphery 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 3 5 13 28 12 19 11 5 4 11 10 18 19 31 17 9 10 0 2 1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0
Endosome 0 4 1 3 14 26 26 7 12 11 3 16 3 8 6 2 3 3 1 2 3 1 3 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0
Mitochondria 22 31 1 6 7 21 14 13 22 12 36 26 6 5 0 34 27 27 0 1 2 0 0 0
Nucleus 36 52 39 16 16 27 17 25 14 14 18 15 24 6 16 39 33 54 18 35 28 5 9 12
Nuclear Periphery 1 4 0 0 0 0 2 1 0 0 0 1 0 0 1 1 0 1 2 0 0 0 0 0
Nucleolus 249 366 217 223 248 379 338 358 266 232 342 357 270 223 210 266 238 267 225 345 206 88 141 161
Peroxisomes 0 1 1 3 4 22 12 1 10 6 3 3 3 8 4 0 0 2 0 0 0 0 0 0
SpindlePole 11 6 31 32 53 134 131 148 119 121 133 167 26 39 38 6 9 13 16 9 13 5 12 22
Vac/Vac Membrane 0 1 0 3 3 1 3 4 0 0 0 0 0 2 0 6 4 5 1 7 1 1 2 4
Unique Cell Count 266 399 241 252 293 425 395 415 320 277 390 402 304 255 256 312 278 314 269 411 264 107 177 211
Labelled Cell Count 326 476 297 305 378 634 580 583 470 417 572 613 358 315 307 374 326 386 269 411 264 107 177 211


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.3 21.0 16.9 16.1 13.4 10.9 10.7 10.5 9.3 9.6 8.8 8.7 18.2 17.0 15.9 24.7 25.5 25.5 13.6 17.0 17.6
Std Deviation (1e-4) 8.2 6.7 5.0 5.1 3.8 3.2 3.0 3.0 2.8 2.8 2.4 2.5 7.6 7.3 6.7 12.2 12.7 14.0 4.1 4.6 5.6
Intensity Change (Log2) – – – -0.07 -0.33 -0.63 -0.66 -0.68 -0.86 -0.82 -0.94 -0.95 0.1 0.0 -0.09 0.55 0.59 0.59 -0.31 0.01 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 2.0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 3.6 0.6 1.8 0.5 0 0 0.6 0.3 2.2 2.8 4.3 2.0 0.8 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.0 3.6 3.7 0 2.6 2.7 0 2.7 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 2.5 2.3 3.8 2.8 4.6 3.7 0 0 0 5.0 4.7 4.4
Nucleus -3.5 -4.1 -4.1 -5.1 -4.2 -4.7 -4.2 -4.9 -5.5 -3.0 -5.4 -3.5 -1.2 -1.4 0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.6 -1.9 -0.3 -1.6 -1.4 -2.3 -2.1 -0.9 -0.5 -0.5 -0.9 -2.6 -1.7 -1.5 -1.8
Peroxisomes 0 0 3.2 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.1 1.7 5.4 5.7 6.3 6.4 7.7 5.9 7.6 -1.6 0.8 0.6 -5.1 -4.1 -3.8
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.7464 15.1917 14.3246 13.6739 14.2694 13.9047 11.5494 14.1647 12.8557 11.164 12.3968 12.1791 13.65 17.3718 15.356 13.7976 14.5213 14.9692
Actin 0.0019 0 0 0 0 0 0.0048 0 0.0005 0.0001 0 0.0001 0.0193 0 0.0097 0.0001 0.0036 0
Bud 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0004 0 0 0 0.0002 0
Bud Neck 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0009 0.0004 0.0001 0.0001 0.0001 0.0001 0.0017 0.0001 0.0001 0.0001 0.0002 0.0001
Bud Periphery 0.0001 0 0 0 0 0 0.0001 0.0001 0 0.0001 0 0 0.0006 0 0 0 0.0003 0
Bud Site 0.0002 0.0001 0 0 0 0 0.0012 0.0002 0.0001 0.0001 0 0.0001 0.0032 0.0001 0.0003 0.0001 0.0006 0
Cell Periphery 0 0 0 0 0.0002 0 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0 0 0 0 0 0 0.0089 0 0.0003 0.0001 0 0 0.0041 0 0 0.0018 0.0005 0
Cytoplasmic Foci 0.0002 0.0001 0.0001 0 0.001 0.0002 0.0135 0.0001 0.0069 0.0017 0.0001 0.0012 0.0182 0 0.0005 0.0026 0.0053 0.0001
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0007 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.0079 0 0 0.0004 0 0 0.0037 0 0 0.0004 0.0008 0
Endosome 0.0002 0 0 0 0 0 0.0109 0 0.0001 0.0316 0 0.0054 0.0111 0 0 0.002 0.0103 0.0001
Golgi 0.0001 0 0 0 0 0 0.0029 0 0.0001 0.0045 0 0.0028 0.0025 0 0.0001 0.0001 0.0014 0
Lipid Particles 0.0005 0 0.0001 0.0001 0.0083 0.0006 0.0095 0.0001 0.0019 0.0142 0.0003 0.0049 0.0095 0 0.0002 0.001 0.0059 0.0002
Mitochondria 0.0004 0 0 0 0.01 0 0.0023 0.0001 0.0003 0.0022 0.0011 0.002 0.0057 0.0001 0.0002 0.0004 0.0078 0.0001
None 0.0001 0 0 0 0 0 0.0055 0 0.0015 0.0002 0 0 0.0091 0 0 0.0029 0.0008 0.0001
Nuclear Periphery 0.0001 0.0002 0 0 0 0 0.0102 0 0.0003 0.0017 0 0.0002 0.0278 0 0 0.0069 0.0074 0.0007
Nucleolus 0.9212 0.861 0.9085 0.8981 0.9095 0.9248 0.8383 0.9098 0.8969 0.849 0.8821 0.9116 0.7873 0.9086 0.906 0.8859 0.8643 0.9384
Nucleus 0.0739 0.137 0.0902 0.1011 0.0688 0.0704 0.0648 0.0884 0.08 0.0883 0.1158 0.0685 0.0554 0.0901 0.0752 0.0834 0.0635 0.0568
Peroxisomes 0.0004 0 0 0 0.0001 0 0.0065 0 0.0082 0.0007 0 0.0004 0.0032 0 0.0069 0.0001 0.0034 0
Punctate Nuclear 0.0004 0.0013 0.0009 0.0003 0.0011 0.0037 0.009 0.0001 0.0019 0.0003 0.0002 0.0018 0.0322 0.0005 0.0001 0.0115 0.0127 0.0032
Vacuole 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0014 0.0006 0.0005 0.0022 0.0001 0.0003 0.0017 0.0002 0.0003 0.0003 0.0059 0.0001
Vacuole Periphery 0 0 0 0 0.0004 0 0.0012 0.0001 0.0002 0.0023 0.0001 0.0003 0.0023 0.0001 0.0001 0.0003 0.0048 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.5294 38.2449 49.1344 43.4559 43.4648 28.0804 40.1123 37.5907 32.5339 43.25
Translational Efficiency 1.4751 1.731 1.2606 1.1807 1.174 1.6294 1.0701 0.7788 0.8531 1.0607

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
Localization
Cell Percentages nucleolus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Utp9

Utp9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Utp9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available