Standard name
Human Ortholog
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.1 0.12 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0.07 0.07 0.07 0.06 0.05 0 0 0 0.1 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.1 0 0 0 0 0 0
Cytoplasm 0.17 0.11 0.06 0.12 0.16 0.19 0.17 0.23 0.25 0.31 0.29 0.34 0.23 0.18 0.18 0 0.1 0 0.11 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0.09 0 0 0 0 0 0 0 0.06 0.17 0.24 0.07 0 0 0.06 0.05 0.1 0.07 0.07 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.06 0.36 0.54 0.33 0 0 0 0 0 0
Mitochondria 0.83 0.87 0.82 0.75 0.66 0.7 0.77 0.71 0.73 0.69 0.75 0.65 0.44 0.38 0.4 0.1 0 0.07 0.67 0.63 0.71 0.73 0.65 0.65
Nucleus 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.09 0.07 0.05 0 0 0 0.06 0 0 0
SpindlePole 0 0 0.06 0.08 0.11 0.1 0.13 0.13 0.07 0.08 0 0.11 0 0.06 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.12 0 0 0.15 0.08 0 0 0 0 0 0 0.16 0.21 0.2 0.18 0.15 0.23 0 0.2 0 0.05 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 1 1 0 0 1 0 0 0 0 8 9 0 2 4 5 1 2 2 2 5 20
Bud 4 2 0 8 11 10 28 34 27 32 19 18 3 6 1 6 1 2 1 0 0 0 4 5
Bud Neck 0 0 0 1 4 3 4 13 7 11 4 5 1 0 2 0 0 0 0 1 0 2 5 9
Bud Site 0 0 0 0 0 2 6 6 5 5 4 8 0 0 0 3 0 2 – – – – – –
Cell Periphery 0 1 0 5 3 4 1 1 1 1 0 2 7 9 9 4 2 4 0 0 0 0 0 1
Cytoplasm 30 14 1 25 54 60 79 120 93 136 92 113 37 47 55 2 4 1 19 3 0 4 8 6
Endoplasmic Reticulum 3 7 0 2 1 0 1 0 1 0 0 0 9 9 4 2 3 3 0 0 0 0 2 10
Endosome 0 3 0 6 30 14 4 5 1 5 1 3 9 46 75 4 1 0 10 6 7 13 30 32
Golgi 1 0 0 2 1 0 0 0 0 0 0 0 8 20 19 22 22 13 5 4 2 1 5 6
Mitochondria 144 111 14 151 223 228 360 363 278 301 236 216 71 100 123 6 1 3 116 86 56 137 267 324
Nucleus 1 1 0 3 5 8 20 22 18 9 7 5 4 8 5 0 1 0 0 1 0 0 3 2
Nuclear Periphery 2 0 1 0 0 0 2 0 0 1 1 0 0 0 0 2 0 2 0 0 0 2 2 6
Nucleolus 2 1 0 1 0 0 4 1 1 0 1 1 0 1 0 2 2 3 0 0 0 2 7 7
Peroxisomes 0 0 0 9 17 9 7 2 2 2 0 3 0 7 29 4 2 1 6 1 5 4 8 14
SpindlePole 2 0 1 16 37 32 62 67 28 35 10 36 7 15 9 1 0 0 1 0 1 2 13 11
Vac/Vac Membrane 11 15 0 6 50 26 17 12 10 14 10 8 26 56 62 11 6 9 4 28 0 8 30 29
Unique Cell Count 174 127 17 202 340 324 470 513 379 437 315 331 162 266 310 61 41 40 174 138 79 189 408 500
Labelled Cell Count 200 157 18 236 437 396 595 647 472 552 385 418 190 333 393 71 49 48 174 138 79 189 408 500


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.8 4.3 5.1 5.0 4.1 3.8 4.1 3.6 3.6 3.3 3.5 6.0 6.5 6.0 9.6 10.9 10.7 5.0 5.4 6.0
Std Deviation (1e-4) 0.7 0.7 0.7 1.5 1.2 1.4 1.5 1.2 1.5 1.3 1.3 1.1 1.0 1.8 1.1 2.1 2.7 2.2 0.8 1.4 1.7
Intensity Change (Log2) – – – 0.24 0.22 -0.06 -0.17 -0.08 -0.27 -0.25 -0.38 -0.28 0.48 0.59 0.48 1.15 1.34 1.32 0.23 0.33 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8486 1.6868 1.4857 1.4037 1.0841 1.6836 1.5029 2.3305 1.7654 1.6127 1.3375 1.7651 0.792 1.1895 0.7894 0.529 0.3806 0.9899
Actin 0.0195 0.003 0.0039 0.0153 0.0017 0.0049 0.0247 0.0019 0.003 0.0112 0.0056 0.0013 0.007 0.0125 0.0029 0.0015 0.0014 0.0011
Bud 0.0006 0.0037 0.0018 0.0085 0.0025 0.0002 0.0007 0.0019 0.0015 0.0017 0.0003 0.0004 0.0037 0.0058 0.0011 0.0041 0.0041 0.0009
Bud Neck 0.0017 0.0006 0.0007 0.0012 0.0007 0.0015 0.0013 0.0005 0.0005 0.0008 0.0003 0.0021 0.0003 0.0006 0.0003 0.0011 0.0023 0.0006
Bud Periphery 0.0016 0.0041 0.0053 0.0343 0.0108 0.0006 0.0016 0.0052 0.003 0.0082 0.0008 0.0012 0.0039 0.0039 0.003 0.0149 0.0072 0.0008
Bud Site 0.004 0.0132 0.0022 0.0024 0.0021 0.0004 0.0042 0.01 0.0046 0.0021 0.0007 0.0003 0.0041 0.0184 0.0052 0.0012 0.0024 0.0003
Cell Periphery 0.0007 0.0008 0.0004 0.0005 0.0009 0.0002 0.0005 0.0013 0.0006 0.0006 0.0001 0.0002 0.0013 0.0036 0.0031 0.0004 0.0005 0.0002
Cytoplasm 0.0076 0.0059 0.0033 0.0198 0.002 0.0088 0.0079 0.0035 0.0054 0.0029 0.0029 0.0033 0.0051 0.0047 0.0052 0.0008 0.0057 0.0045
Cytoplasmic Foci 0.0217 0.0232 0.0182 0.0276 0.0274 0.0115 0.0415 0.0375 0.0216 0.0098 0.0382 0.0234 0.0157 0.0354 0.0195 0.0139 0.0253 0.0158
Eisosomes 0.0007 0.0002 0.0002 0.0001 0.0002 0.0002 0.0005 0.0003 0.0002 0.0004 0.0001 0.0001 0.0004 0.001 0.0004 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0037 0.0035 0.0011 0.001 0.0009 0.0011 0.0025 0.0006 0.0011 0.0019 0.0032 0.0005 0.0013 0.0006 0.0007 0.0002 0.0003 0.0004
Endosome 0.0897 0.089 0.0846 0.073 0.0652 0.0442 0.0619 0.0795 0.056 0.0575 0.1057 0.0355 0.066 0.0979 0.0566 0.0413 0.1222 0.045
Golgi 0.0353 0.043 0.0288 0.0473 0.0426 0.0329 0.0353 0.0526 0.0197 0.0225 0.0329 0.0272 0.0435 0.0772 0.0301 0.0312 0.0425 0.0395
Lipid Particles 0.0544 0.0109 0.0149 0.0274 0.032 0.0291 0.0444 0.0265 0.0112 0.0137 0.0226 0.0186 0.0199 0.022 0.0099 0.0131 0.0071 0.0094
Mitochondria 0.6873 0.7402 0.7683 0.7004 0.7148 0.8163 0.6797 0.6925 0.8218 0.818 0.7181 0.8391 0.7765 0.6523 0.8268 0.8478 0.7345 0.8566
None 0.0126 0.0054 0.0025 0.0132 0.0078 0.0013 0.0094 0.0006 0.0019 0.0046 0.0022 0.0055 0.0079 0.0033 0.0039 0.0003 0.0005 0.0063
Nuclear Periphery 0.0186 0.004 0.0029 0.0014 0.0015 0.0036 0.0065 0.0005 0.0009 0.0038 0.0137 0.0005 0.0022 0.0003 0.0005 0.0001 0.0001 0.0002
Nucleolus 0.0016 0.0068 0.0041 0.0006 0.0014 0.0004 0.0034 0.0015 0.0007 0.001 0.0006 0.0004 0.0008 0.0011 0.0006 0.0003 0.0005 0.0006
Nucleus 0.0048 0.0011 0.0017 0.0009 0.001 0.0007 0.0033 0.0011 0.0006 0.0021 0.0022 0.0004 0.0007 0.0006 0.0036 0.0003 0.0005 0.0005
Peroxisomes 0.0138 0.0204 0.0243 0.0118 0.0676 0.0313 0.0439 0.0465 0.023 0.018 0.0349 0.0321 0.0144 0.0441 0.015 0.0197 0.0272 0.0103
Punctate Nuclear 0.0043 0.0004 0.0008 0.0031 0.0042 0.0012 0.0082 0.0002 0.0022 0.0043 0.0056 0.0004 0.0004 0.0003 0.0004 0.0001 0.0001 0.0004
Vacuole 0.0083 0.0119 0.0197 0.0055 0.0079 0.0044 0.0122 0.0252 0.0143 0.0081 0.0055 0.0035 0.0153 0.0089 0.0067 0.0044 0.0089 0.0028
Vacuole Periphery 0.0076 0.0085 0.0104 0.0046 0.0048 0.0052 0.0064 0.0107 0.0062 0.0064 0.004 0.0039 0.0096 0.0053 0.0046 0.0033 0.0066 0.0037

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.5751 52.8605 53.3456 45.1031 25.3002 43.7335 66.5417 56.9957 61.7622 43.8868
Translational Efficiency 0.6078 0.6605 0.5503 0.7189 0.8604 0.7097 0.5902 0.8936 0.7473 0.7171

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
Localization
Cell Percentages mitochondrion (93%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Aim46

Aim46


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim46-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available