Standard name
Human Ortholog
Description Evolutionarily conserved 19S regulatory particle assembly-chaperone; involved in assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress; ortholog of human p27
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.98 0.97 0.94 0.9 0.89 – 0.79 0.78 0.75 0.71 0.99 0.98 0.99 0.95 0.91 0.98 0.93 0.92 0.93 0.9 0.9 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.19 0 – 0.18 0.12 0.21 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.06 0 0.11 – 0.14 0.12 0.1 0.13 0 0 0 0 0.09 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 – 0 1 4 1 0 0 0 0 0 0 1 3 2 1 3 8
Bud 0 1 0 0 3 7 0 – 6 8 8 11 0 0 0 0 0 0 2 3 2 5 17 23
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 4
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 4 4 18 3 21 – 15 32 14 34 4 3 2 3 4 5 0 0 0 0 1 0
Cytoplasm 390 560 525 376 743 510 769 – 491 624 506 543 464 495 595 103 101 108 372 541 501 334 675 695
Endoplasmic Reticulum 4 6 2 0 0 0 1 – 3 10 5 5 0 1 1 2 2 3 0 1 2 2 6 4
Endosome 1 0 2 0 1 0 4 – 1 2 1 8 1 1 0 0 2 0 4 6 3 2 8 4
Golgi 1 0 1 0 0 0 0 – 0 0 0 0 1 3 0 0 0 1 5 2 2 4 0 5
Mitochondria 3 8 1 10 12 108 31 – 109 94 141 166 0 1 0 3 3 3 3 11 4 2 3 2
Nucleus 0 2 0 1 2 5 5 – 10 9 5 3 0 0 0 3 1 1 0 2 0 1 3 2
Nuclear Periphery 0 0 0 0 0 0 1 – 3 3 0 9 0 0 0 1 0 0 0 0 0 1 0 2
Nucleolus 0 0 0 1 0 0 1 – 0 0 1 3 1 0 0 0 0 0 0 0 0 2 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 2 – 0 0 1 0 0 0 0 0 0 1 2 3 7 3 7 12
Vac/Vac Membrane 12 1 19 19 49 21 99 – 84 93 68 103 5 3 5 4 10 0 3 4 2 1 9 6
Unique Cell Count 401 575 537 388 787 566 862 620 800 674 768 468 503 600 108 111 110 402 590 537 371 748 784
Labelled Cell Count 412 581 554 412 828 654 934 722 876 754 886 476 507 603 119 123 122 402 590 537 371 748 784


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.2 6.4 6.5 7.4 5.6 7.1 – 6.4 7.0 6.7 6.9 7.0 7.0 7.3 8.3 9.2 8.3 5.8 6.0 6.4
Std Deviation (1e-4) 0.9 0.8 1.1 1.4 1.3 1.5 1.4 – 1.3 1.4 1.5 1.6 1.4 1.4 1.5 1.6 2.0 1.6 1.5 1.4 1.6
Intensity Change (Log2) – – – 0.04 0.21 -0.19 0.15 – 0.01 0.13 0.08 0.11 0.13 0.14 0.19 0.37 0.53 0.39 -0.14 -0.08 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.2 0 2.3 – 2.2 3.6 1.9 3.9 0 0 0 0 0 0
Cytoplasm -0.8 -3.0 -5.3 -5.9 – -9.6 -10.2 -11.0 -12.5 1.7 0.8 1.9 -1.4 -3.6 0.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.4 10.5 4.1 – 10.1 8.1 11.1 11.4 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 2.2 0.2 5.2 – 6.0 5.2 4.4 6.0 -2.6 -3.3 -3.2 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2292 0.8787 0.5211 0.8625 0.4651 0.618 1.8727 1.8933 1.7577 1.9273 1.348 1.9108 4.7557 5.2932 4.8498 4.7241 4.4296 4.9955
Actin 0.0113 0.0044 0.0052 0.0115 0.0025 0.0032 0.0422 0.0031 0.0177 0.0007 0.0432 0.021 0.0002 0.0004 0.0004 0.0002 0.0018 0.0013
Bud 0.0082 0.019 0.0218 0.0236 0.0358 0.0139 0.0011 0.0014 0.0068 0.0016 0.0247 0.0004 0 0.0001 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0009 0.0007 0.0022 0.0017 0.0029 0.0059 0.0037 0.0002 0.0011 0.0005 0.0015 0.0019 0.0001 0.0002 0.0001 0.0001 0.0003 0.0005
Bud Periphery 0.0031 0.0056 0.0072 0.0094 0.0118 0.0062 0.002 0.0018 0.0048 0.0012 0.0201 0.0007 0 0 0.0001 0 0.0001 0.0001
Bud Site 0.0102 0.0208 0.017 0.0089 0.0014 0.002 0.0061 0.0042 0.0154 0.0018 0.0104 0.0009 0.0001 0.0017 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0.0004 0.0004 0.0005 0.0003 0.0004 0.0003 0.0003 0.0003 0.0003 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.4576 0.5634 0.456 0.3444 0.4857 0.5283 0.3791 0.4898 0.5287 0.6053 0.4004 0.6153 0.6354 0.7846 0.6923 0.4916 0.8225 0.7582
Cytoplasmic Foci 0.0391 0.0237 0.0209 0.0307 0.03 0.0379 0.0307 0.0072 0.0268 0.0115 0.0164 0.0102 0.0035 0.0031 0.0032 0.0046 0.0135 0.0077
Eisosomes 0.0002 0.0001 0.0001 0.0002 0 0 0.0005 0.0001 0.0002 0 0.0002 0.0002 0 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0041 0.0022 0.0028 0.0032 0.003 0.0023 0.0079 0.0031 0.0043 0.0036 0.003 0.0026 0.0056 0.0023 0.0024 0.0021 0.0065 0.0032
Endosome 0.0123 0.0029 0.0074 0.0083 0.0204 0.0066 0.0207 0.0016 0.0094 0.0061 0.0063 0.0062 0.0016 0.0008 0.0012 0.0024 0.0074 0.0019
Golgi 0.002 0.0002 0.001 0.0025 0.0003 0.0004 0.0063 0.0003 0.0029 0.0002 0.0022 0.0037 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001
Lipid Particles 0.0025 0.0005 0.0033 0.0052 0.0004 0.0004 0.0093 0.0003 0.0046 0.0001 0.0029 0.0041 0.0001 0.0001 0.0002 0.0001 0.001 0.0001
Mitochondria 0.0031 0.0007 0.0026 0.0037 0.0015 0.0018 0.0034 0.0004 0.0023 0.0004 0.0015 0.0042 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001
None 0.4177 0.3201 0.4078 0.4372 0.293 0.3255 0.4377 0.4779 0.3399 0.3539 0.447 0.3013 0.3503 0.2015 0.2963 0.4933 0.1368 0.2219
Nuclear Periphery 0.0054 0.0027 0.0031 0.0119 0.0102 0.0037 0.0097 0.0012 0.0067 0.0024 0.002 0.0047 0.0008 0.0009 0.0006 0.0009 0.0024 0.0007
Nucleolus 0.0017 0.0008 0.002 0.0032 0.0023 0.0017 0.0012 0.0002 0.0008 0.0002 0.0004 0.0003 0 0 0.0001 0.0001 0.0001 0
Nucleus 0.0067 0.0128 0.0149 0.0299 0.0426 0.0221 0.0103 0.0028 0.0061 0.0052 0.0037 0.0109 0.001 0.0026 0.0012 0.0019 0.0028 0.0024
Peroxisomes 0.0014 0.0003 0.0047 0.004 0.0007 0.0007 0.0117 0.0004 0.0103 0.0002 0.0043 0.0071 0 0 0.0001 0.0001 0.0004 0.0001
Punctate Nuclear 0.0092 0.0169 0.0168 0.0573 0.0505 0.0346 0.0125 0.0025 0.0088 0.0027 0.0084 0.0032 0.0002 0.0007 0.0004 0.0011 0.0014 0.0009
Vacuole 0.0023 0.0014 0.0023 0.0021 0.0037 0.0019 0.0027 0.0012 0.0012 0.0016 0.0009 0.0007 0.0005 0.0006 0.0007 0.001 0.0017 0.0004
Vacuole Periphery 0.0006 0.0002 0.0004 0.0009 0.0008 0.0005 0.001 0.0001 0.0006 0.0003 0.0003 0.0005 0.0001 0.0001 0.0001 0.0002 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.8177 5.951 6.6238 11.812 7.5523 3.3267 12.0341 10.6604 15.369 12.4757
Translational Efficiency 1.0008 1.9436 1.6711 1.6824 1.742 5.2704 1.3612 1.5955 1.1639 1.4132

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
262 1155 2319 1921 1465 1989 3019 1960 1727 3144 5338 3881

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 724.55 762.50 887.25 833.29 688.53 811.09 858.06 951.65 693.99 793.24 870.74 893.06
Standard Deviation 103.26 119.15 117.69 148.22 80.76 105.46 122.76 134.46 85.55 113.14 121.45 153.32
Intensity Change Log 2 — 0.073652 0.292255 0.201733 — 0.236342 0.317559 0.466912 — 0.155217 0.304640 0.337032

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000268 0.000270 0.000147 0.000399 0.000086 0.000050 0.000203 0.000459 0.000114 0.000131 0.000179 0.000429
Bud Neck 0.011206 0.014964 0.007108 0.006010 0.004279 0.005467 0.006780 0.004423 0.005330 0.008956 0.006923 0.005209
Bud Site 0.013599 0.013935 0.009446 0.031584 0.002273 0.002372 0.014133 0.025347 0.003992 0.006620 0.012097 0.028434
Cell Periphery 0.000330 0.000119 0.000072 0.000185 0.000106 0.000075 0.000083 0.000101 0.000140 0.000091 0.000078 0.000142
Cytoplasm 0.523674* 0.557864* 0.505820* 0.661609* 0.574817* 0.637722* 0.590178* 0.635236* 0.567058* 0.608385* 0.553530* 0.648290*
Cytoplasmic Foci 0.234053* 0.159922 0.003840 0.014437 0.154861 0.081190 0.005150 0.011711 0.166875 0.110113 0.004581 0.013060
Eisosomes 0.000129 0.000064 0.000011 0.000023 0.000075 0.000034 0.000012 0.000020 0.000083 0.000045 0.000012 0.000022
Endoplasmic Reticulum 0.001604 0.001582 0.001230 0.001357 0.000678 0.000629 0.001037 0.001200 0.000819 0.000979 0.001121 0.001278
Endosome 0.010967 0.013151 0.000716 0.001127 0.005296 0.003348 0.000606 0.002288 0.006157 0.006949 0.000653 0.001713
Golgi 0.003085 0.002020 0.000178 0.001053 0.000859 0.000571 0.000113 0.000807 0.001197 0.001103 0.000141 0.000929
Lipid Particles 0.005606 0.003525 0.000325 0.000620 0.004610 0.001394 0.000354 0.000521 0.004761 0.002177 0.000341 0.000570
Mitochondria 0.002817 0.003802 0.000689 0.000518 0.001511 0.001008 0.001154 0.000974 0.001709 0.002035 0.000952 0.000748
Mitotic Spindle 0.008425 0.002482 0.006053 0.007616 0.000666 0.000580 0.012147 0.023934 0.001843 0.001279 0.009500 0.015857
None 0.005908 0.005124 0.006227 0.008310 0.005065 0.007821 0.005390 0.004849 0.005193 0.006830 0.005753 0.006562
Nuclear Periphery 0.000414 0.000387 0.001230 0.000483 0.000465 0.000372 0.001130 0.000762 0.000458 0.000377 0.001174 0.000624
Nuclear Periphery Foci 0.001095 0.001106 0.000434 0.001159 0.000704 0.000266 0.000277 0.000780 0.000763 0.000575 0.000346 0.000967
Nucleolus 0.002231 0.001449 0.000256 0.000221 0.000919 0.000793 0.000225 0.000192 0.001118 0.001034 0.000238 0.000206
Nucleus 0.114543 0.141102* 0.428461* 0.185194* 0.204260* 0.223207* 0.327159* 0.205709* 0.190649* 0.193044* 0.371168* 0.195555*
Peroxisomes 0.003526 0.004377 0.000090 0.000251 0.002033 0.001008 0.000105 0.001040 0.002259 0.002245 0.000099 0.000649
Vacuole 0.053799 0.070576 0.027194 0.077600 0.035974 0.031663 0.033481 0.077931 0.038678 0.045958 0.030750 0.077767
Vacuole Periphery 0.002720 0.002179 0.000472 0.000243 0.000463 0.000431 0.000282 0.001715 0.000805 0.001073 0.000365 0.000986

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.05 1.35 -2.03 -2.86 -4.09 3.15 -4.48 -3.61 -4.04 -2.06 -1.08 -3.41 -5.77 -5.52 -4.05
Bud Neck -1.31 1.86 2.52 6.69 1.66 -1.90 -4.10 0.04 1.97 4.22 -4.68 -2.10 1.29 6.21 3.80
Bud Site -0.04 1.76 -1.61 -2.47 -5.49 -0.22 -7.41 -7.67 -7.60 -1.60 -3.16 -5.62 -9.21 -7.41 -5.08
Cell Periphery 3.03 3.64 2.73 -0.99 -3.66 4.79 3.81 3.94 -0.76 0.08 4.05 5.16 2.36 -1.81 -3.40
Cytoplasm -1.74 4.38 -2.73 -1.57 -14.73 -7.17 5.21 5.30 13.20 0.65 -5.17 10.02 2.37 8.97 -9.37
Cytoplasmic Foci 5.53 18.58 17.70 27.33 -14.84 14.03 32.77 31.35 25.22 -8.46 11.28 37.30 35.33 35.89 -16.25
Eisosomes 5.53 9.95 8.78 10.75 -9.85 9.50 13.78 11.36 4.59 -9.50 9.14 16.69 13.74 10.80 -13.58
Endoplasmic Reticulum 0.04 -0.65 0.05 0.00 2.34 0.26 -9.10 -7.51 -3.89 0.70 -1.10 -8.84 -7.10 -3.63 1.93
Endosome -1.23 7.25 7.05 11.75 -2.36 4.33 12.14 9.36 6.55 -3.89 -1.27 13.94 12.31 13.44 -4.65
Golgi 1.52 4.22 3.26 3.39 -1.09 0.94 3.97 2.44 0.93 -2.07 0.43 5.50 2.87 2.58 -1.66
Lipid Particles 2.63 6.97 6.42 9.51 -7.84 6.03 7.73 7.25 6.26 -8.15 5.45 9.21 8.51 11.10 -10.95
Mitochondria -0.80 3.17 3.61 3.88 4.84 1.33 1.41 2.50 1.06 2.82 -0.63 2.84 4.06 4.05 4.78
Mitotic Spindle 1.53 0.43 0.14 -4.08 -0.73 0.12 -7.79 -7.75 -7.93 -2.03 0.76 -6.66 -7.53 -9.16 -1.89
None 0.25 0.03 0.05 -0.72 0.18 -2.28 0.08 -0.76 2.21 -2.21 -1.46 -0.21 -0.57 1.45 -0.98
Nuclear Periphery 0.23 -13.17 -4.14 -9.88 16.86 1.11 -13.66 -11.87 -20.88 2.09 1.08 -17.44 -10.21 -23.01 11.94
Nuclear Periphery Foci -0.06 0.90 -0.79 -2.19 -6.54 3.90 2.31 -1.29 -11.13 -6.68 1.44 1.88 -3.03 -7.65 -9.11
Nucleolus 0.67 1.35 1.38 4.95 1.34 0.86 4.62 4.47 6.07 -0.94 0.46 3.07 3.13 7.32 0.72
Nucleus -2.47 -31.07 -8.20 -8.96 35.95 -2.96 -22.41 -5.57 -2.77 17.12 -0.65 -36.10 -5.73 -5.91 36.47
Peroxisomes -1.03 5.66 5.49 10.28 -3.40 2.63 5.43 3.93 2.22 -1.95 0.12 6.85 5.87 9.26 -2.47
Vacuole -3.16 0.08 -14.88 -15.15 -20.34 1.68 -10.10 -25.88 -26.96 -19.56 -3.77 -8.89 -33.11 -31.20 -27.98
Vacuole Periphery 0.61 2.69 2.95 4.69 1.08 0.53 4.18 -0.63 -0.76 -1.60 -1.18 3.44 2.07 3.14 -0.90
Standard name
Human Ortholog
Description Evolutionarily conserved 19S regulatory particle assembly-chaperone; involved in assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress; ortholog of human p27
Localization
Cell Percentages cytoplasm (54%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nas2

Nas2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nas2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available