Standard name
Human Ortholog
Description Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.07 0.05 0.08 0.07 0.07 0 0 0.05 0.09 0.12 0.21 0.13 0.12 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.13 0 0.05 0 0.23 0.55 0.19 0.5 0.34 0.45 0.43 0 0 0 0.12 0.07 0.05 0 0 0 0 0 0
Nucleus 0.91 0.91 0.93 0.94 0.86 0.8 0.75 0.83 0.76 0.83 0.83 0.79 0.89 0.87 0.76 0.78 0.8 0.75 0.79 0.83 0.81 0.77 0.78 0.67
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0 0 0 0 0 0 0.05 0.06 0.06 0.08 0.1 0 0 0 0 0 0 0.08 0.05 0.07 0.08 0.08 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.1 0.06 0.06 0 0.06 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 1 0 0 3 0 2 2 2 1 0 1 0 1 0 0 3 4 1 0 1 4
Bud Neck 0 0 0 1 0 1 0 0 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 12 10 2 7 6 19 19 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 11 9 8 5 16 17 26 25 18 12 11 16 17 25 47 17 11 7 4 1 0 0 3 1
Endoplasmic Reticulum 0 1 1 0 0 0 1 0 0 0 0 0 0 0 2 2 1 7 0 0 1 0 0 2
Endosome 1 5 3 2 5 3 0 0 0 2 0 0 1 1 1 2 0 2 3 4 3 3 0 4
Golgi 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 2 0 0 1
Mitochondria 29 58 3 9 7 76 171 73 133 135 136 150 1 1 1 15 7 5 2 4 0 0 1 2
Nucleus 299 416 195 178 211 266 231 316 201 327 247 274 171 187 167 101 76 69 267 410 178 99 119 131
Nuclear Periphery 3 1 0 1 1 3 0 4 0 4 3 1 0 0 1 0 1 0 0 1 1 0 0 1
Nucleolus 46 5 0 2 2 7 5 19 17 23 25 35 0 1 0 3 1 3 27 26 14 9 11 18
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 3 3 11 3 6 6 2 5 0 3 3 4 5 2 4 2 2 2 5 7 2 2 3 7
Vac/Vac Membrane 3 7 3 2 9 5 0 6 3 5 0 1 3 0 3 7 6 9 19 27 9 7 8 15
Unique Cell Count 328 458 209 190 246 334 310 382 264 392 299 348 192 216 220 130 95 92 338 496 222 129 154 195
Labelled Cell Count 397 506 224 204 258 396 449 451 381 521 447 502 198 218 226 150 105 104 338 496 222 129 154 195


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.8 6.0 5.4 5.9 4.8 3.5 5.1 4.1 4.5 4.2 4.3 5.8 5.5 5.5 5.6 6.6 7.0 5.8 6.5 6.4
Std Deviation (1e-4) 0.8 0.7 1.3 1.2 1.6 1.1 1.1 1.2 1.0 0.9 1.0 0.8 1.2 1.0 1.5 1.3 1.5 1.5 1.4 1.8 1.5
Intensity Change (Log2) – – – -0.15 -0.01 -0.3 -0.77 -0.22 -0.54 -0.39 -0.5 -0.48 -0.04 -0.11 -0.11 -0.09 0.16 0.22 -0.03 0.13 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8559 2.2329 2.2655 1.9392 1.5591 2.0216 3.991 4.3078 4.3007 4.1032 3.9131 4.3873 2.0094 1.9253 1.6795 1.6381 1.9212 1.7563
Actin 0.0118 0.0046 0.0065 0.0003 0 0.0063 0.0045 0 0.0008 0.0002 0.0021 0.0017 0.0842 0 0.008 0.0022 0.0038 0.0004
Bud 0.0001 0.0001 0.0004 0.0001 0 0.0001 0.0002 0 0 0.0003 0.0002 0 0.0003 0 0.0002 0.0001 0.0002 0
Bud Neck 0.0107 0.0004 0.0033 0.0007 0.0003 0.0022 0.0005 0.0001 0.0002 0.001 0.0006 0.0007 0.0067 0.0002 0.0011 0.0007 0.0008 0.0035
Bud Periphery 0.0003 0.0002 0.0006 0.0003 0 0.0001 0.0006 0 0.0001 0.0012 0.001 0.0001 0.0005 0 0.0002 0.0003 0.0003 0.0001
Bud Site 0.0025 0.0133 0.0097 0.0005 0 0.0002 0.0005 0 0.0001 0.0025 0.0002 0 0.0018 0.0001 0.0024 0.0008 0.0003 0.0001
Cell Periphery 0.0002 0.0002 0.0001 0.0001 0 0 0.0001 0 0 0.0007 0.0001 0 0.0002 0 0.0001 0.0001 0 0
Cytoplasm 0.007 0.0005 0.0061 0.0014 0.0002 0.0007 0.0019 0.0002 0.0009 0.0002 0.014 0.0003 0.0075 0.0018 0.0011 0.0006 0.0018 0.0004
Cytoplasmic Foci 0.0034 0.0007 0.0086 0.0033 0 0.0075 0.0002 0 0.0004 0.0008 0.0027 0.0003 0.011 0 0.0006 0.0009 0.0005 0.0002
Eisosomes 0.0002 0.0001 0.0001 0 0 0.0001 0.0001 0 0 0 0.0001 0 0.0013 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.002 0.0005 0.0012 0.0006 0 0.0003 0.0006 0 0.0002 0.0002 0.0073 0.0005 0.0043 0 0.0001 0.0014 0.0013 0.0001
Endosome 0.0074 0.0056 0.0037 0.0293 0 0.0076 0.0019 0 0.0017 0.0063 0.0203 0.0039 0.0207 0 0.0006 0.0112 0.0051 0.0008
Golgi 0.0015 0.0008 0.0038 0.008 0 0.0072 0.0007 0 0.0005 0.0015 0.0039 0.0007 0.0091 0 0.0008 0.0015 0.0004 0.0002
Lipid Particles 0.0025 0.003 0.001 0.0242 0 0.0067 0.0004 0 0.0017 0.0097 0.0025 0.0003 0.0216 0 0.0008 0.0017 0.0003 0.0006
Mitochondria 0.0023 0.0025 0.0134 0.0046 0.0001 0.0038 0.0037 0 0.0041 0.0035 0.0125 0.0003 0.0024 0.0001 0.0007 0.0022 0.0028 0.0002
None 0.0245 0.0002 0.0112 0.0007 0.0001 0.0003 0.008 0.0001 0.0055 0.0002 0.0018 0.0002 0.0242 0.0044 0.0007 0.0006 0.0013 0.0002
Nuclear Periphery 0.0107 0.0061 0.0139 0.0087 0.0039 0.0024 0.0059 0.0015 0.0064 0.0014 0.0301 0.0027 0.0214 0.0015 0.0078 0.0105 0.0366 0.003
Nucleolus 0.026 0.0289 0.0206 0.0272 0.028 0.0414 0.017 0.0134 0.0154 0.0219 0.0347 0.0389 0.0132 0.0182 0.0156 0.0082 0.0431 0.0375
Nucleus 0.8463 0.9283 0.8664 0.8794 0.9374 0.8944 0.9395 0.9741 0.9513 0.9388 0.8485 0.9442 0.7273 0.9625 0.9363 0.9506 0.8923 0.9354
Peroxisomes 0.0012 0.0003 0.0068 0.0009 0 0.0085 0.0002 0 0.0003 0.0005 0.0001 0.0001 0.0123 0 0.0008 0.0004 0.0002 0.0001
Punctate Nuclear 0.0354 0.0011 0.0215 0.0047 0.0299 0.0096 0.0128 0.0105 0.0099 0.0012 0.008 0.0047 0.028 0.0109 0.0217 0.0029 0.0073 0.0166
Vacuole 0.0027 0.0012 0.0005 0.0034 0 0.0003 0.0004 0 0.0002 0.0065 0.0036 0.0002 0.001 0.0001 0.0001 0.0018 0.0006 0.0002
Vacuole Periphery 0.0012 0.0013 0.0007 0.0013 0 0.0002 0.0004 0 0.0002 0.0015 0.0057 0.0002 0.0009 0 0.0002 0.0013 0.0011 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2215 792 186 86 1850 2390 159 1683 4065 3182 345 1769

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 789.23 712.80 869.12 931.88 668.21 862.62 944.74 873.64 734.15 825.33 903.97 876.47
Standard Deviation 91.81 165.55 86.34 98.08 79.35 100.13 97.49 114.49 105.31 136.19 99.09 114.43
Intensity Change Log 2 — -0.146948 0.139110 0.239698 — 0.368424 0.499616 0.386737 — 0.112300 0.315627 0.308978

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000093 0.000694 0.000798 0.001876 0.000181 0.000151 0.001287 0.001410 0.000133 0.000286 0.001023 0.001432
Bud Neck 0.002348 0.006830 0.002934 0.007757 0.003217 0.006145 0.007552 0.028301 0.002744 0.006315 0.005063 0.027303
Bud Site 0.000349 0.002497 0.001649 0.027342 0.000946 0.001034 0.001104 0.014067 0.000621 0.001398 0.001398 0.014713
Cell Periphery 0.000051 0.000277 0.000086 0.000114 0.000119 0.000064 0.000055 0.000154 0.000082 0.000117 0.000072 0.000152
Cytoplasm 0.008375 0.032316 0.003955 0.037994 0.019075 0.008403 0.001343 0.020787 0.013244 0.014355 0.002751 0.021624
Cytoplasmic Foci 0.001772 0.007158 0.000929 0.001594 0.001643 0.002412 0.000104 0.001453 0.001713 0.003594 0.000549 0.001460
Eisosomes 0.000014 0.000040 0.000060 0.000082 0.000017 0.000017 0.000052 0.000030 0.000015 0.000023 0.000057 0.000032
Endoplasmic Reticulum 0.001572 0.004096 0.004994 0.014013 0.001849 0.002659 0.010253 0.007955 0.001698 0.003017 0.007418 0.008250
Endosome 0.000500 0.001251 0.000407 0.001437 0.000532 0.000793 0.000360 0.002585 0.000515 0.000907 0.000385 0.002529
Golgi 0.000256 0.000737 0.000122 0.000132 0.000151 0.000425 0.000100 0.000925 0.000208 0.000503 0.000111 0.000886
Lipid Particles 0.000778 0.002977 0.000986 0.001351 0.000592 0.000887 0.000496 0.000443 0.000693 0.001407 0.000760 0.000487
Mitochondria 0.001991 0.002002 0.000937 0.001513 0.001394 0.003478 0.001110 0.007970 0.001719 0.003111 0.001017 0.007656
Mitotic Spindle 0.001113 0.003198 0.001261 0.006792 0.001298 0.003535 0.000291 0.020003 0.001198 0.003451 0.000814 0.019360
None 0.003765 0.064205 0.004914 0.002679 0.016813 0.006488 0.002536 0.007016 0.009703 0.020854 0.003818 0.006805
Nuclear Periphery 0.000890 0.000795 0.000462 0.001882 0.000498 0.001393 0.000745 0.002931 0.000712 0.001244 0.000593 0.002880
Nuclear Periphery Foci 0.000375 0.002864 0.000298 0.001764 0.000405 0.000726 0.000791 0.000996 0.000388 0.001258 0.000525 0.001034
Nucleolus 0.017336 0.042639 0.016368 0.005531 0.021291 0.025629 0.017972 0.007222 0.019136 0.029863 0.017107 0.007140
Nucleus 0.957050* 0.822034* 0.953900* 0.851452* 0.928925* 0.932688* 0.947614* 0.865371* 0.944250* 0.905146* 0.951003* 0.864694*
Peroxisomes 0.000560 0.001493 0.000520 0.000618 0.000410 0.001132 0.000417 0.000616 0.000492 0.001221 0.000473 0.000616
Vacuole 0.000625 0.001498 0.004180 0.033810 0.000494 0.001398 0.005652 0.008477 0.000565 0.001423 0.004858 0.009709
Vacuole Periphery 0.000186 0.000398 0.000239 0.000267 0.000151 0.000543 0.000165 0.001289 0.000170 0.000507 0.000205 0.001239

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.23 -7.64 -6.67 -4.78 -3.68 1.11 -9.23 -4.29 -4.39 1.56 -6.47 -11.83 -4.94 -4.25 0.21
Bud Neck -5.11 -3.14 -5.90 -1.66 -2.29 -4.33 -5.54 -16.05 -14.10 -9.25 -7.09 -6.15 -16.91 -14.18 -12.00
Bud Site -5.31 -2.05 -1.52 -1.24 -1.21 -0.47 -2.68 -6.59 -6.50 -5.68 -3.24 -2.82 -7.07 -6.40 -5.22
Cell Periphery -6.58 -3.02 -5.47 3.10 -1.74 3.59 2.50 -0.33 -3.78 -2.76 -3.03 -1.07 -2.86 -0.54 -1.75
Cytoplasm -6.83 3.50 -2.39 -0.03 -2.81 5.63 9.96 -0.59 -6.20 -10.43 -0.99 9.77 -4.15 -3.16 -10.19
Cytoplasmic Foci -3.45 1.55 -0.57 2.76 -1.30 -1.57 4.81 2.27 3.22 -2.91 -3.46 3.80 2.61 4.90 -0.90
Eisosomes -7.38 -6.11 -7.00 -5.26 -2.99 -0.71 -7.51 -10.70 -10.40 4.66 -6.22 -9.18 -13.55 -8.26 4.90
Endoplasmic Reticulum -9.16 -6.02 -7.89 -6.79 -5.66 -2.32 -9.61 -18.53 -13.72 2.96 -4.97 -10.89 -20.46 -15.15 -0.85
Endosome -2.86 -0.90 -4.18 -2.50 -3.72 -0.90 -0.67 -3.23 -2.89 -3.25 -2.07 -1.11 -4.16 -2.86 -3.64
Golgi -2.88 1.06 0.77 3.61 -0.83 -1.82 0.75 -2.04 -0.31 -2.24 -2.40 1.25 -1.66 0.17 -2.12
Lipid Particles -2.19 -1.51 -3.45 0.86 -1.30 -1.57 -0.90 1.12 2.63 1.71 -2.51 -1.74 1.56 3.14 2.49
Mitochondria -0.04 1.40 0.75 1.76 -1.32 -3.17 -0.14 -4.47 -2.01 -4.57 -2.47 1.25 -4.01 -2.41 -4.75
Mitotic Spindle -1.66 -0.52 -2.28 -1.92 -2.17 -1.89 0.08 -7.31 -6.18 -7.41 -2.56 -0.39 -7.73 -6.52 -7.48
None -9.77 -1.30 0.35 9.70 1.26 6.69 10.15 7.72 1.14 -4.06 -6.29 5.94 4.81 8.67 -1.72
Nuclear Periphery 0.57 2.59 -5.93 -5.99 -6.63 -4.30 -4.71 -12.36 -7.77 -8.50 -3.28 -2.15 -12.01 -8.98 -10.87
Nuclear Periphery Foci -3.96 -0.57 -3.43 0.72 -3.19 -1.43 -1.63 -2.86 -0.93 0.34 -3.85 -1.71 -3.38 0.97 -0.87
Nucleolus -7.97 -0.49 9.44 12.25 5.36 -2.61 1.56 10.37 13.48 2.26 -7.53 0.85 12.46 17.25 4.60
Nucleus 13.84 2.23 5.80 0.72 5.06 -0.47 0.33 10.46 10.98 6.04 10.18 1.77 15.01 6.79 9.63
Peroxisomes -2.63 0.13 0.55 3.39 0.67 -2.49 -0.04 0.16 2.46 0.20 -2.79 0.12 0.96 3.35 1.11
Vacuole -3.44 -2.64 -4.66 -4.61 -4.26 -2.99 -3.30 -10.93 -10.15 0.33 -3.54 -4.14 -11.42 -10.84 -2.29
Vacuole Periphery -3.75 -0.82 -2.31 2.01 0.01 -3.42 -1.80 -3.10 -0.92 -2.76 -3.83 -1.21 -3.03 -1.08 -2.43
Standard name
Human Ortholog
Description Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Snp1

Snp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available