Standard name
Human Ortholog
Description Protein required for synthesis of diphthamide; required along with Dph2p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.1 0 0 0 0 0 0
Cytoplasm 0.97 1.0 0.98 0.95 0.96 0.85 0.78 0.79 0.74 0.72 0.69 0.62 1.0 1.0 1.0 0.93 0.98 0.96 0.94 0.97 0.92 0.95 0.97 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.13 0.08 0.31 0.41 0.34 0.39 0.49 0.52 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 1 0 1 2 4 9 25 12 13 27 20 14 0 0 1 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0
Bud Site 0 0 0 0 0 0 1 0 0 0 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 16 10 3 7 16 13 12 18 13 11 8 15 12 11 11 16 0 0 0 0 0 0
Cytoplasm 172 62 326 285 329 462 545 542 398 484 312 276 305 433 515 153 161 162 191 73 311 325 413 426
Endoplasmic Reticulum 0 0 6 0 0 0 1 2 1 1 0 13 0 2 2 9 6 7 1 0 9 3 1 1
Endosome 0 0 0 0 0 1 2 5 2 4 0 3 0 0 0 0 0 0 2 0 0 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0
Mitochondria 4 0 3 38 28 168 288 236 206 331 236 244 0 0 0 4 2 1 1 0 3 0 1 2
Nucleus 0 0 0 0 1 2 2 3 0 4 2 0 0 0 1 1 1 1 1 0 0 4 0 1
Nuclear Periphery 0 0 1 0 0 0 2 0 1 1 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 2 1 3 10 11 15 23 23 12 8 0 0 1 7 1 2 2 0 1 2 1 1
Unique Cell Count 178 62 334 299 342 541 699 690 535 676 454 448 305 435 517 164 164 168 204 76 338 343 425 444
Labelled Cell Count 180 62 357 336 368 659 894 831 658 894 598 574 313 450 532 185 182 190 204 76 338 343 425 444


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 8.8 8.1 7.3 6.5 5.0 5.1 5.0 4.8 4.4 4.2 4.1 9.7 9.4 8.9 13.6 12.6 13.7 9.4 9.6 9.4
Std Deviation (1e-4) 1.0 1.0 1.2 1.5 1.3 1.3 1.3 1.2 1.1 0.9 1.1 0.9 1.3 1.1 1.0 2.7 2.1 2.5 1.1 1.3 1.3
Intensity Change (Log2) – – – -0.14 -0.31 -0.69 -0.65 -0.69 -0.76 -0.87 -0.94 -0.96 0.27 0.22 0.14 0.75 0.64 0.76 0.23 0.26 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.9 -3.1 -3.1 -2.2 -2.6 0 0 0 0 -1.4 -0.9 -2.0 0.9 0.9 2.0
Cytoplasm -1.6 -1.1 -5.9 -8.1 -8.0 -8.9 -9.8 -10.2 -11.8 2.7 2.3 2.7 -2.4 0.4 -0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.0 4.5 10.9 13.5 11.8 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.8 3.2 2.7 2.1 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7737 5.3908 4.6931 4.4527 4.194 4.8259 2.5202 3.8477 3.376 3.1102 2.9572 3.339 2.2708 2.3635 2.8995 2.1031 3.0575 2.9474
Actin 0.0012 0.0003 0.0006 0.0002 0.0003 0.0004 0.0176 0.0018 0.0144 0.0174 0.0252 0.0029 0.0237 0.0005 0.0058 0.0076 0.0001 0.0007
Bud 0.0002 0.0021 0.0003 0.0002 0.0002 0.0001 0.0004 0.0007 0.0009 0.0004 0.0025 0.0007 0.0003 0.001 0.0039 0.0172 0.0001 0.0029
Bud Neck 0.0002 0.0003 0.0003 0.0003 0.0003 0.0006 0.0006 0.0002 0.0013 0.0008 0.0009 0.0006 0.0036 0.0002 0.0023 0.0005 0.0002 0.0006
Bud Periphery 0.0001 0.0009 0.0002 0.0001 0 0.0001 0.0005 0.0003 0.0007 0.0002 0.0015 0.0031 0.0004 0.0004 0.0017 0.0091 0 0.0011
Bud Site 0.0011 0.006 0.0017 0.0002 0.0001 0.0001 0.001 0.0122 0.0099 0.0007 0.0016 0.0003 0.002 0.0057 0.0041 0.0059 0 0.0002
Cell Periphery 0.0004 0.0004 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0.0003 0.0001 0.0002 0.0003 0.0002 0.0005 0 0
Cytoplasm 0.8687 0.9364 0.9294 0.9087 0.9426 0.9278 0.7652 0.939 0.9035 0.8014 0.6563 0.9325 0.8731 0.8927 0.9376 0.841 0.9672 0.9534
Cytoplasmic Foci 0.012 0.0049 0.008 0.012 0.0154 0.0096 0.0316 0.0112 0.0124 0.0159 0.0518 0.0154 0.0136 0.012 0.0091 0.0256 0.0077 0.01
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0002 0 0.0002 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0124 0.0011 0.0036 0.0055 0.0029 0.0043 0.0236 0.0047 0.0029 0.0042 0.0032 0.006 0.0159 0.0036 0.0042 0.004 0.0052 0.0035
Endosome 0.0141 0.0029 0.0093 0.038 0.0202 0.0119 0.0541 0.0102 0.0149 0.0164 0.0645 0.0198 0.0231 0.0105 0.0146 0.0183 0.0119 0.0125
Golgi 0.0011 0.0005 0.0008 0.0008 0.0006 0.0007 0.0219 0.0008 0.0038 0.003 0.0367 0.0014 0.0041 0.0005 0.002 0.0059 0.0003 0.0005
Lipid Particles 0.0015 0 0.0003 0 0.0001 0.0002 0.0212 0.0002 0.0026 0.0019 0.0707 0.0008 0.0059 0.0003 0.0011 0.0011 0 0.0001
Mitochondria 0.0003 0.0001 0.0002 0.0003 0.0002 0.0009 0.004 0.0002 0.0024 0.0014 0.0102 0.0018 0.0021 0.0002 0.0005 0.0057 0.0001 0.0006
None 0.0778 0.0405 0.0398 0.0223 0.0092 0.0397 0.0304 0.013 0.0037 0.0216 0.0163 0.0032 0.0158 0.0649 0.0052 0.0315 0.0026 0.0101
Nuclear Periphery 0.0018 0.0002 0.0007 0.0006 0.0006 0.0004 0.005 0.0007 0.0009 0.0048 0.0102 0.0027 0.0035 0.0008 0.0013 0.0015 0.0005 0.0005
Nucleolus 0.0002 0 0.0001 0 0 0 0.0015 0.0001 0.0011 0.0007 0.001 0.0001 0.0002 0.0001 0.0001 0.0007 0 0
Nucleus 0.0012 0.001 0.0011 0.0008 0.0006 0.0007 0.0015 0.001 0.0123 0.1014 0.0028 0.0011 0.0018 0.0012 0.0011 0.0024 0.0007 0.001
Peroxisomes 0.0002 0 0.0001 0 0.0001 0.0001 0.0054 0.0002 0.007 0.0014 0.0227 0.0032 0.0041 0.0001 0.0005 0.0116 0 0.0001
Punctate Nuclear 0.0009 0.0001 0.0004 0.0001 0.0003 0.0003 0.0099 0.0008 0.0008 0.0029 0.0162 0.0013 0.003 0.0005 0.0012 0.0011 0.0001 0.0002
Vacuole 0.0042 0.002 0.0024 0.0082 0.0054 0.0017 0.0032 0.0023 0.0035 0.0025 0.003 0.002 0.0027 0.0041 0.0026 0.0072 0.0027 0.0017
Vacuole Periphery 0.0004 0.0002 0.0003 0.0015 0.0008 0.0003 0.0011 0.0003 0.0005 0.0007 0.0022 0.0009 0.0007 0.0005 0.0006 0.0014 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.1529 19.2953 21.1906 17.7242 24.6271 8.6291 24.9721 16.3278 12.7845 23.5935
Translational Efficiency 1.7533 2.0389 1.2264 1.2277 1.1037 3.1529 1.2393 1.1154 1.3656 1.098

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2114 1012 1802 108 1692 2024 195 744 3806 3036 1997 852

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 913.22 965.24 1024.34 1358.76 885.84 987.49 1231.53 1563.41 901.05 980.07 1044.57 1537.47
Standard Deviation 108.99 152.16 168.05 152.90 111.92 123.13 123.97 234.41 111.14 133.92 175.40 235.76
Intensity Change Log 2 — 0.079925 0.165660 0.573256 — 0.156720 0.475334 0.819578 — 0.118249 0.326441 0.699797

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000228 0.000240 0.000131 0.001169 0.000172 0.000201 0.000297 0.000496 0.000203 0.000214 0.000148 0.000582
Bud Neck 0.003822 0.002585 0.000390 0.001994 0.003484 0.002635 0.001336 0.002588 0.003672 0.002619 0.000483 0.002513
Bud Site 0.015020 0.027838 0.004058 0.033911 0.016130 0.019287 0.018694 0.010423 0.015513 0.022137 0.005487 0.013400
Cell Periphery 0.000167 0.000103 0.000039 0.000349 0.000164 0.000102 0.000100 0.000066 0.000165 0.000103 0.000045 0.000102
Cytoplasm 0.623965* 0.712700* 0.897497* 0.753181* 0.629750* 0.720446* 0.605543* 0.917636* 0.626537* 0.717864* 0.868988* 0.896789*
Cytoplasmic Foci 0.217423* 0.162031 0.019822 0.086652 0.209248* 0.158862 0.099568 0.005943 0.213789* 0.159918 0.027609 0.016174
Eisosomes 0.000075 0.000017 0.000004 0.000018 0.000055 0.000023 0.000026 0.000004 0.000066 0.000021 0.000006 0.000006
Endoplasmic Reticulum 0.004778 0.000562 0.000283 0.000354 0.003027 0.001032 0.000535 0.000020 0.004000 0.000875 0.000307 0.000062
Endosome 0.019237 0.016400 0.000990 0.003264 0.018241 0.011869 0.012204 0.001033 0.018794 0.013379 0.002085 0.001316
Golgi 0.015220 0.007021 0.000202 0.000860 0.009706 0.008076 0.001316 0.001021 0.012769 0.007725 0.000311 0.001001
Lipid Particles 0.002612 0.000759 0.000285 0.002415 0.002365 0.001130 0.001042 0.000139 0.002502 0.001006 0.000359 0.000428
Mitochondria 0.002247 0.000566 0.000046 0.000004 0.002330 0.001432 0.000016 0.000323 0.002284 0.001143 0.000043 0.000282
Mitotic Spindle 0.001472 0.001507 0.002579 0.000306 0.002164 0.005804 0.001082 0.000433 0.001780 0.004372 0.002432 0.000417
None 0.001568 0.001204 0.002594 0.000090 0.001108 0.000895 0.000274 0.000473 0.001363 0.000998 0.002368 0.000424
Nuclear Periphery 0.000050 0.000025 0.000071 0.000036 0.000078 0.000031 0.000135 0.000010 0.000062 0.000029 0.000078 0.000013
Nuclear Periphery Foci 0.000321 0.000744 0.001648 0.005442 0.000314 0.000370 0.007367 0.000044 0.000318 0.000495 0.002206 0.000728
Nucleolus 0.000180 0.000160 0.000071 0.000039 0.000158 0.000135 0.000070 0.000178 0.000170 0.000143 0.000071 0.000160
Nucleus 0.008676 0.005658 0.039778 0.005839 0.009729 0.006455 0.014132 0.005956 0.009144 0.006190 0.037274 0.005941
Peroxisomes 0.001144 0.001790 0.000116 0.000937 0.001097 0.001130 0.002206 0.000837 0.001124 0.001350 0.000320 0.000850
Vacuole 0.074710 0.054684 0.029133 0.102961 0.084370 0.056871 0.233747* 0.051775 0.079005 0.056142 0.049113 0.058264
Vacuole Periphery 0.007084 0.003404 0.000261 0.000180 0.006311 0.003217 0.000310 0.000602 0.006741 0.003279 0.000266 0.000548

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.61 2.53 -5.24 -5.01 -5.76 -1.30 -2.70 -1.15 -1.05 -0.75 -0.70 1.64 -1.43 -1.37 -1.55
Bud Neck 3.29 10.55 3.35 0.69 -5.76 2.52 9.19 2.82 0.33 -3.81 3.84 15.46 3.76 0.43 -7.72
Bud Site -6.81 13.43 -5.20 -1.20 -8.44 -2.11 -0.53 4.65 6.87 2.51 -6.09 12.14 2.73 7.28 -6.15
Cell Periphery 6.71 17.68 -3.42 -4.99 -6.55 7.47 6.40 11.61 6.20 3.78 10.41 22.12 8.45 1.64 -4.96
Cytoplasm -9.22 -39.20 -3.60 0.37 7.88 -11.12 2.49 -38.05 -28.41 -16.60 -15.17 -37.89 -39.90 -24.81 -5.58
Cytoplasmic Foci 8.31 48.75 16.53 8.76 -8.54 8.78 15.01 45.25 40.64 13.36 12.80 58.54 61.14 42.81 6.95
Eisosomes 6.88 8.41 6.48 -0.54 -5.32 9.93 7.64 16.48 15.54 9.91 9.24 12.22 12.39 14.72 1.56
Endoplasmic Reticulum 6.07 6.45 6.48 3.45 0.50 5.03 7.15 8.63 5.29 7.26 7.20 8.84 9.51 6.36 9.00
Endosome 1.88 22.02 16.45 9.35 -4.02 5.77 3.36 18.21 15.17 4.04 6.32 25.85 26.30 17.95 1.94
Golgi 8.11 27.27 25.98 7.34 -3.80 2.21 12.58 14.29 9.08 0.04 8.18 34.77 21.77 10.43 -1.29
Lipid Particles 15.66 20.68 -1.25 -4.40 -5.10 7.37 7.77 18.90 7.80 6.75 13.09 25.41 16.78 3.73 -1.55
Mitochondria 3.40 5.18 5.27 2.18 8.97 1.15 3.45 3.02 2.75 -2.94 2.42 5.89 5.24 3.02 -2.56
Mitotic Spindle -0.09 -1.06 0.51 0.44 1.53 -2.43 1.43 2.14 4.02 1.33 -2.55 -0.63 2.47 4.04 2.70
None 0.52 -1.38 3.36 1.96 7.13 1.08 5.05 4.19 3.49 -2.36 1.14 -1.99 3.80 2.90 6.10
Nuclear Periphery 2.59 -4.96 -1.35 -3.29 1.88 1.67 -2.72 2.09 1.62 10.28 2.40 -3.11 2.65 0.51 14.78
Nuclear Periphery Foci -3.08 -11.06 -7.01 -6.39 -4.99 -1.07 -10.01 9.23 6.74 10.41 -2.94 -14.38 -3.92 -2.33 8.43
Nucleolus 0.70 7.39 10.11 4.87 2.70 0.16 9.90 -2.72 -1.55 -8.83 0.67 9.32 -0.71 -1.05 -7.57
Nucleus 6.33 -22.35 2.36 -1.30 21.59 7.88 -3.57 4.17 -2.06 5.07 10.01 -22.24 4.07 -2.72 22.98
Peroxisomes -2.45 16.73 1.71 3.06 -5.38 -0.33 -0.50 1.64 1.78 0.88 -2.18 8.56 1.95 2.92 -2.38
Vacuole 5.15 9.10 -4.70 -5.83 -6.48 7.67 -10.27 5.86 -1.09 11.60 8.99 3.42 1.65 -4.32 -1.30
Vacuole Periphery 6.22 15.16 15.57 8.18 1.02 4.71 10.84 9.80 6.72 -1.23 7.89 18.22 16.05 8.79 -1.22
Standard name
Human Ortholog
Description Protein required for synthesis of diphthamide; required along with Dph2p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Dph1

Dph1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dph1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available