Standard name | |
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Human Ortholog | |
Description | AdoMet-dependent methyltransferase; involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 3 | 3 | 7 | 12 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 0 | 4 | 13 | 31 | 26 |
Bud Neck | 1 | 0 | 10 | 5 | 8 | 5 | 5 | 16 | 4 | 9 | 2 | 13 | 1 | 2 | 3 | 0 | 0 | 0 | 3 | 1 | 0 | 2 | 5 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 3 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 3 | 2 | 6 | 6 |
Cytoplasm | 624 | 114 | 338 | 330 | 445 | 419 | 406 | 409 | 410 | 382 | 239 | 307 | 417 | 518 | 567 | 10 | 16 | 13 | 483 | 104 | 230 | 373 | 484 | 479 |
Endoplasmic Reticulum | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 7 | 11 | 5 | 0 | 2 | 3 | 1 | 3 |
Endosome | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 2 | 1 | 7 | 4 | 10 | 2 | 3 | 3 | 5 | 5 |
Golgi | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Mitochondria | 33 | 1 | 0 | 2 | 4 | 32 | 82 | 41 | 105 | 78 | 154 | 82 | 1 | 0 | 0 | 3 | 2 | 7 | 26 | 7 | 26 | 13 | 26 | 17 |
Nucleus | 32 | 13 | 11 | 15 | 17 | 10 | 13 | 10 | 7 | 13 | 11 | 8 | 18 | 21 | 9 | 99 | 150 | 159 | 51 | 16 | 10 | 24 | 39 | 19 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 2 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
SpindlePole | 0 | 0 | 2 | 2 | 4 | 0 | 7 | 4 | 6 | 8 | 4 | 13 | 0 | 0 | 3 | 0 | 2 | 0 | 19 | 0 | 13 | 13 | 29 | 21 |
Vac/Vac Membrane | 25 | 16 | 5 | 2 | 4 | 2 | 6 | 0 | 3 | 2 | 3 | 4 | 7 | 18 | 12 | 29 | 65 | 56 | 27 | 9 | 8 | 15 | 26 | 25 |
Unique Cell Count | 696 | 137 | 357 | 348 | 468 | 440 | 466 | 443 | 471 | 450 | 347 | 383 | 447 | 552 | 584 | 135 | 228 | 225 | 683 | 145 | 324 | 483 | 684 | 647 |
Labelled Cell Count | 719 | 145 | 373 | 357 | 484 | 471 | 521 | 483 | 539 | 496 | 421 | 433 | 452 | 562 | 598 | 144 | 256 | 252 | 683 | 145 | 324 | 483 | 684 | 647 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.4 | 4.3 | 4.6 | 4.3 | 4.3 | 3.5 | 3.4 | 3.9 | 3.1 | 3.2 | 2.9 | 3.1 | 4.7 | 5.1 | 5.0 | 8.1 | 9.0 | 8.5 | 4.6 | 4.7 | 5.2 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.5 | 1.9 | 1.7 | 1.5 | 1.7 | 1.6 | 1.4 | 0.8 | 1.2 | 0.9 | 1.3 | 1.6 | 1.7 | 1.4 | 1.9 | 1.6 | 1.6 | 1.5 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.1 | -0.41 | -0.45 | -0.26 | -0.58 | -0.53 | -0.67 | -0.56 | 0.01 | 0.14 | 0.1 | 0.81 | 0.96 | 0.88 | -0.02 | 0.03 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 7.8 | 9.9 | 9.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5304 | 0.836 | 0.8315 | 0.5739 | 0.3509 | 0.5768 | -0.5302 | -0.124 | -0.3974 | -0.6541 | -0.5937 | -0.5384 | 0.5012 | -0.485 | -0.2451 | -0.1783 | 0.0388 | -0.3376 |
Actin | 0.0077 | 0.0002 | 0.0097 | 0.0027 | 0.0261 | 0.001 | 0.0126 | 0.001 | 0.0015 | 0.0001 | 0.0001 | 0.0003 | 0.03 | 0.0067 | 0.0046 | 0.0075 | 0.0417 | 0.0037 |
Bud | 0.0005 | 0.0017 | 0.0004 | 0.0005 | 0.0011 | 0.0018 | 0.0007 | 0.0007 | 0.0004 | 0.0006 | 0.0001 | 0.0002 | 0.0058 | 0.0016 | 0.009 | 0.0013 | 0.0103 | 0.0025 |
Bud Neck | 0.0025 | 0.0004 | 0.0036 | 0.0017 | 0.0039 | 0.0031 | 0.0041 | 0.0006 | 0.0005 | 0.0004 | 0.0003 | 0.0111 | 0.0044 | 0.0015 | 0.0009 | 0.0057 | 0.0015 | 0.0026 |
Bud Periphery | 0.0013 | 0.0008 | 0.0008 | 0.0008 | 0.0026 | 0.0038 | 0.0012 | 0.0005 | 0.0004 | 0.0006 | 0.0001 | 0.0001 | 0.004 | 0.0012 | 0.006 | 0.0044 | 0.0446 | 0.001 |
Bud Site | 0.0024 | 0.0015 | 0.0075 | 0.0018 | 0.002 | 0.0004 | 0.0031 | 0.006 | 0.0044 | 0.0007 | 0.0001 | 0.0011 | 0.0071 | 0.0234 | 0.0101 | 0.006 | 0.0019 | 0.0008 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0001 |
Cytoplasm | 0.0731 | 0.061 | 0.0441 | 0.0268 | 0.0668 | 0.0964 | 0.0557 | 0.0419 | 0.0576 | 0.0389 | 0.0645 | 0.0725 | 0.0834 | 0.1289 | 0.0857 | 0.057 | 0.0778 | 0.1373 |
Cytoplasmic Foci | 0.0105 | 0.0047 | 0.0081 | 0.0074 | 0.0053 | 0.0113 | 0.0164 | 0.0086 | 0.0119 | 0.0015 | 0.0018 | 0.007 | 0.047 | 0.0141 | 0.0089 | 0.0292 | 0.0084 | 0.0166 |
Eisosomes | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0042 | 0.0011 | 0.0009 | 0.0018 | 0.0015 | 0.0011 | 0.0017 | 0.001 | 0.0011 | 0.0004 | 0.0003 | 0.0008 | 0.011 | 0.0017 | 0.0028 | 0.0016 | 0.0019 | 0.0015 |
Endosome | 0.0087 | 0.0015 | 0.0056 | 0.0403 | 0.0065 | 0.0042 | 0.008 | 0.0006 | 0.0037 | 0.0003 | 0.0002 | 0.0008 | 0.0415 | 0.0089 | 0.009 | 0.0174 | 0.0216 | 0.0074 |
Golgi | 0.0014 | 0.0005 | 0.0034 | 0.0159 | 0.0036 | 0.0015 | 0.0027 | 0.0001 | 0.0007 | 0 | 0 | 0 | 0.0079 | 0.0003 | 0.0032 | 0.0138 | 0.0072 | 0.003 |
Lipid Particles | 0.0064 | 0.0021 | 0.0034 | 0.0015 | 0.0225 | 0.0034 | 0.0112 | 0.0002 | 0.0016 | 0.0001 | 0 | 0.0002 | 0.0426 | 0.0009 | 0.0097 | 0.0481 | 0.0136 | 0.0058 |
Mitochondria | 0.0051 | 0.0007 | 0.0104 | 0.0104 | 0.0013 | 0.0016 | 0.0058 | 0.0003 | 0.0066 | 0.0005 | 0.0002 | 0.0003 | 0.0081 | 0.0011 | 0.0081 | 0.0267 | 0.0086 | 0.0025 |
None | 0.586 | 0.5545 | 0.5929 | 0.6974 | 0.5407 | 0.5271 | 0.6923 | 0.674 | 0.6115 | 0.5844 | 0.6488 | 0.659 | 0.3711 | 0.5737 | 0.5384 | 0.272 | 0.5066 | 0.4601 |
Nuclear Periphery | 0.0167 | 0.0061 | 0.0051 | 0.0081 | 0.0098 | 0.0062 | 0.007 | 0.0042 | 0.012 | 0.0027 | 0.0015 | 0.0028 | 0.0494 | 0.006 | 0.0107 | 0.015 | 0.0151 | 0.0109 |
Nucleolus | 0.0049 | 0.003 | 0.0051 | 0.0025 | 0.0069 | 0.0063 | 0.0032 | 0.0026 | 0.0037 | 0.0048 | 0.0016 | 0.004 | 0.0071 | 0.0044 | 0.0016 | 0.0043 | 0.003 | 0.003 |
Nucleus | 0.2192 | 0.3318 | 0.274 | 0.1659 | 0.2518 | 0.2778 | 0.1315 | 0.2419 | 0.2614 | 0.3497 | 0.2691 | 0.2159 | 0.2019 | 0.1862 | 0.2423 | 0.318 | 0.2103 | 0.2878 |
Peroxisomes | 0.0101 | 0.0056 | 0.0092 | 0.0011 | 0.0015 | 0.0024 | 0.0197 | 0.0004 | 0.001 | 0 | 0 | 0.0001 | 0.0232 | 0.0025 | 0.029 | 0.036 | 0.0088 | 0.0067 |
Punctate Nuclear | 0.0345 | 0.0213 | 0.0139 | 0.0084 | 0.0439 | 0.0484 | 0.02 | 0.0144 | 0.0176 | 0.0127 | 0.0111 | 0.023 | 0.0366 | 0.0329 | 0.0155 | 0.1318 | 0.0055 | 0.0449 |
Vacuole | 0.0034 | 0.0013 | 0.001 | 0.0032 | 0.0011 | 0.0017 | 0.0019 | 0.0008 | 0.0017 | 0.0013 | 0.0002 | 0.0006 | 0.0103 | 0.0031 | 0.0022 | 0.0025 | 0.0078 | 0.0012 |
Vacuole Periphery | 0.001 | 0.0002 | 0.0004 | 0.0015 | 0.0006 | 0.0004 | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0065 | 0.0005 | 0.0017 | 0.0016 | 0.0032 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 37.4613 | 36.5325 | 38.7266 | 32.694 | 25.2313 | 30.1469 | 31.0015 | 24.6338 | 19.6753 | 33.0558 |
Translational Efficiency | 0.9942 | 0.8906 | 0.8431 | 0.7206 | 1.0508 | 0.8337 | 0.944 | 0.9286 | 1.1158 | 0.7913 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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