Standard name
Human Ortholog
Description Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase complex; catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.11 0.09 0.11
Bud 0 0 0.05 0.07 0.06 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Golgi 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.41 0.32 0.12 0.05 0.09 0.05 0.15 0.1 0.16
Mitochondria 0.79 0.91 0.85 0.79 0.62 0.81 0.8 0.81 0.87 0.89 0.87 0.92 0.87 0.88 0.85 0.12 0.11 0.06 0.82 0.7 0.71 0.51 0.45 0.43
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0 0.05 0 0.14 0.16 0.18 0.16 0.15 0.14 0.14 0.12 0.07 0 0 0.12 0.21 0.44 0 0 0 0 0 0
Peroxisomes 0.09 0.05 0.14 0.29 0.31 0.1 0.06 0.06 0 0 0.05 0 0.1 0.08 0.08 0.29 0.26 0.19 0.06 0 0.11 0.12 0.26 0.16
SpindlePole 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.06 0.26 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.08 0.06 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.06 0.21 0.12 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 6 4 0 0 2 3 0 3 0 2 1 1 2 0 0 0 5 17 22 18 22 29
Bud 1 7 25 21 26 18 22 17 25 13 15 13 8 3 8 0 0 0 0 2 4 0 2 2
Bud Neck 0 0 1 1 0 2 4 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 1 2 0 3 4 9 4 4 2 5 1 1 0 1 0 0 1 – – – – – –
Cell Periphery 2 6 15 1 1 1 0 0 1 0 1 1 1 0 2 1 0 0 0 1 0 0 0 0
Cytoplasm 0 5 4 1 6 8 7 15 14 7 11 7 5 7 7 0 0 0 0 1 0 0 0 2
Endoplasmic Reticulum 0 1 1 0 0 0 0 1 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 2
Endosome 5 3 8 2 15 2 2 4 0 0 1 0 1 3 6 1 1 0 1 10 19 7 7 12
Golgi 14 9 5 0 1 0 1 1 0 1 0 1 3 14 1 7 6 2 12 30 27 25 24 43
Mitochondria 159 284 392 224 247 319 409 479 371 474 309 442 190 198 235 2 2 1 195 232 353 85 113 120
Nucleus 1 0 2 0 1 1 2 1 2 0 3 0 1 0 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 6 12 12 3 5 3 13 7 5 6 4 9 1 2 5 0 0 0 1 2 2 1 1 0
Nucleolus 25 11 22 12 57 62 90 94 65 72 48 58 16 5 8 2 4 7 2 1 1 1 2 4
Peroxisomes 19 16 65 84 126 39 32 38 14 20 16 15 22 19 22 5 5 3 13 11 53 20 66 45
SpindlePole 3 3 13 12 23 17 12 16 8 2 5 11 3 0 0 1 5 1 0 2 4 1 4 7
Vac/Vac Membrane 21 25 26 4 4 4 11 11 6 10 4 7 12 15 16 1 4 2 3 16 4 3 1 1
Unique Cell Count 202 313 463 285 401 396 509 590 428 532 355 480 219 226 276 17 19 16 238 333 497 168 253 280
Labelled Cell Count 257 387 599 369 515 480 616 691 515 611 422 567 266 268 314 20 27 18 238 333 497 168 253 280


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.0 14.7 11.3 11.4 14.5 15.2 16.4 17.5 16.9 18.6 16.9 18.7 15.2 14.6 13.5 18.1 20.4 19.3 17.2 18.5 18.9
Std Deviation (1e-4) 4.1 3.8 3.0 2.4 5.2 4.8 5.4 5.5 5.4 6.9 6.8 7.1 4.3 3.3 3.3 4.2 7.5 6.3 4.5 5.1 5.1
Intensity Change (Log2) – – – 0.01 0.36 0.42 0.53 0.63 0.58 0.72 0.58 0.73 0.43 0.36 0.26 0.68 0.85 0.77 0.6 0.71 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1.1 0.7 -0.6 -0.8 -2.1 0.3 -2.4 -0.8 -2.1 -1.0 -2.5 -1.6 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2.0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.7 -3.3 -3.2 -4.1 -4.4 -3.4 -4.2 -3.0 -3.6 -2.2 0 -2.2 0 0 0
Cytoplasm 0 0 1.4 0.8 2.0 2.6 0.7 2.4 0.8 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 1.8 0 0 -1.6 0 0 0 0 0 0 0.4 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 3.8 0 0 0 0
Mitochondria -2.1 -7.7 -1.6 -1.8 -1.5 0.9 2.1 1.0 3.6 0.7 1.0 0.2 -7.7 -8.2 -8.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.5 -1.4 -2.0 0 -1.7 -1.6 -1.7 -1.5 -0.7 0 0 -0.7 0 0 0
Nucleolus -0.3 4.8 5.4 6.3 5.8 5.2 4.7 4.4 4.0 1.4 -1.6 -1.2 0 0 0
Peroxisomes 5.1 6.1 -1.9 -4.0 -4.1 -5.6 -5.8 -4.5 -6.0 -1.5 -2.1 -2.5 0 0 0
SpindlePole 1.0 2.1 1.2 -0.4 -0.1 -0.9 -3.1 -1.4 -0.5 -1.2 0 0 0 0 0
Vacuole -2.9 -3.7 -3.7 -2.8 -3.3 -3.4 -3.1 -3.4 -3.5 -0.1 0.5 0.1 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.84 25.9941 22.6509 20.11 22.9125 23.4243 21.3619 23.5881 22.7356 21.229 21.2181 21.9038 27.0195 28.9639 25.222 26.5163 25.6347 26.6325
Actin 0.0046 0.0096 0.0092 0.002 0.0067 0.0073 0.0047 0.0081 0.0043 0.0015 0.0056 0.0038 0.0133 0.0169 0.0074 0.003 0.0336 0.0026
Bud 0.0002 0.0002 0.0006 0 0.0001 0.0001 0 0.0002 0 0 0 0 0 0.0002 0 0 0.0001 0
Bud Neck 0.0006 0.001 0.0016 0.0001 0.0002 0.0007 0.0001 0.0008 0.0001 0.0045 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0
Bud Periphery 0.0002 0.0002 0.0006 0 0.0002 0.0001 0.0001 0.0003 0 0 0 0 0.0002 0.0002 0 0 0.0002 0
Bud Site 0.0022 0.0056 0.0059 0.0001 0.0006 0.0002 0.0002 0.0023 0.0001 0.0002 0.0001 0.0001 0.0002 0.0028 0.0001 0.0001 0.0001 0.0001
Cell Periphery 0.0003 0.0003 0.0005 0.0001 0.0002 0.0003 0.0002 0.0005 0.0002 0.0007 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002 0.0002
Cytoplasm 0.0001 0 0.0001 0 0.001 0 0 0.0001 0 0 0 0 0.0001 0.0001 0 0 0 0
Cytoplasmic Foci 0.0687 0.1165 0.0799 0.1122 0.0484 0.0687 0.0992 0.1135 0.1028 0.1112 0.0904 0.0597 0.0606 0.0778 0.0625 0.1405 0.1424 0.0219
Eisosomes 0.0003 0.0003 0.0003 0.0003 0.0003 0.0005 0.0004 0.0005 0.0003 0.0004 0.0036 0.0003 0.0014 0.0005 0.0014 0.0002 0.0004 0.0003
Endoplasmic Reticulum 0.0004 0 0.0001 0 0.001 0 0 0 0 0 0 0 0.0002 0 0 0 0 0
Endosome 0.0078 0.0052 0.0031 0.001 0.0111 0.0024 0.0028 0.0059 0.002 0.0072 0.0023 0.0011 0.0005 0.0039 0.0009 0.0006 0.0004 0.0009
Golgi 0.01 0.0109 0.0158 0.0029 0.0037 0.016 0.0174 0.0259 0.0164 0.0451 0.0178 0.0089 0.0031 0.0173 0.0096 0.002 0.0038 0.0077
Lipid Particles 0.1532 0.1343 0.1045 0.1141 0.1463 0.1409 0.1702 0.1723 0.1285 0.1047 0.2205 0.1922 0.1004 0.0851 0.0933 0.0641 0.1008 0.1062
Mitochondria 0.5523 0.4667 0.5272 0.5167 0.4614 0.56 0.5099 0.4344 0.5593 0.5595 0.5212 0.5748 0.6364 0.6025 0.6437 0.5589 0.4947 0.7
None 0.0001 0 0.0001 0 0.0004 0 0 0 0 0 0.0004 0 0.0002 0.0001 0 0 0.0001 0
Nuclear Periphery 0.0031 0.0001 0.0001 0 0.0094 0 0.0001 0.0001 0 0 0 0 0.0001 0 0 0 0 0
Nucleolus 0.0058 0.0038 0.0026 0.0013 0.0008 0.0052 0.0065 0.0046 0.0046 0.0046 0.0069 0.0086 0.0016 0.0008 0.0015 0.0007 0.0303 0.0012
Nucleus 0.0004 0.0002 0.0002 0 0.0019 0.0002 0.0002 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0 0 0.0005 0.0001
Peroxisomes 0.181 0.2323 0.238 0.2469 0.2755 0.1895 0.1802 0.2114 0.1764 0.137 0.1251 0.145 0.1747 0.1874 0.1776 0.2287 0.1911 0.1483
Punctate Nuclear 0.0005 0.0001 0.0014 0.0001 0.0025 0.0001 0.0004 0.0002 0.0001 0.0002 0.0001 0.0001 0.0044 0.0001 0.0001 0.0001 0.0002 0
Vacuole 0.0022 0.0027 0.0022 0.0003 0.0067 0.0009 0.001 0.0027 0.0006 0.0033 0.0006 0.0005 0.0003 0.0006 0.0002 0.0002 0.0002 0.0012
Vacuole Periphery 0.0059 0.0099 0.0061 0.0017 0.0217 0.0069 0.0062 0.016 0.0042 0.0194 0.0047 0.0043 0.0016 0.0032 0.0014 0.0006 0.0009 0.0092

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 68.6684 65.7584 66.1024 76.9935 66.2451 56.1356 71.2374 68.4179 69.0617 72.5527
Translational Efficiency 1.0532 1.0136 0.9616 0.8615 0.9387 1.3532 0.9089 0.8338 0.9474 0.9226

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase complex; catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA
Localization
Cell Percentages mitochondrion (94%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Kgd1

Kgd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kgd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available