Standard name | |
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Human Ortholog | |
Description | Component of the kinetochore-associated Ndc80 complex; conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, and kinetochore assembly and clustering; evolutionarily conserved; complex members include Ndc80p, Nuf2p, Scp24p, and Spc25p; modified by sumoylation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 |
Bud | 7 | 4 | 2 | 3 | 4 | 3 | 5 | 18 | 11 | 12 | 9 | 5 | 0 | 0 | 0 | 9 | 4 | 3 | 2 | 4 | 1 | 2 | 2 | 7 |
Bud Neck | 8 | 6 | 11 | 4 | 8 | 12 | 8 | 10 | 11 | 15 | 11 | 5 | 1 | 3 | 3 | 3 | 4 | 2 | 0 | 5 | 3 | 0 | 0 | 3 |
Bud Site | 11 | 3 | 2 | 3 | 1 | 9 | 13 | 12 | 15 | 20 | 12 | 8 | 3 | 1 | 1 | 10 | 5 | 9 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 13 | 12 | 7 | 14 | 13 | 13 | 17 | 10 | 11 | 9 | 8 | 17 | 45 | 80 | 35 | 29 | 26 | 0 | 1 | 0 | 1 | 2 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 4 | 2 | 0 | 0 | 1 | 0 | 0 | 1 |
Endosome | 16 | 44 | 9 | 7 | 10 | 13 | 8 | 10 | 12 | 6 | 7 | 15 | 7 | 41 | 69 | 45 | 44 | 31 | 16 | 18 | 9 | 9 | 19 | 10 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 3 | 4 | 2 | 0 | 1 | 0 |
Mitochondria | 16 | 5 | 0 | 3 | 1 | 5 | 8 | 4 | 8 | 12 | 29 | 7 | 2 | 0 | 1 | 30 | 13 | 11 | 2 | 3 | 0 | 0 | 4 | 3 |
Nucleus | 14 | 14 | 4 | 8 | 16 | 12 | 16 | 19 | 10 | 11 | 12 | 11 | 4 | 5 | 15 | 29 | 30 | 30 | 0 | 1 | 0 | 2 | 4 | 3 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 1 | 1 |
Nucleolus | 300 | 302 | 136 | 68 | 137 | 159 | 135 | 190 | 149 | 185 | 112 | 154 | 120 | 149 | 180 | 245 | 183 | 170 | 10 | 34 | 13 | 3 | 12 | 7 |
Peroxisomes | 28 | 32 | 5 | 3 | 5 | 11 | 3 | 9 | 16 | 8 | 16 | 9 | 0 | 8 | 30 | 19 | 14 | 6 | 5 | 5 | 3 | 3 | 1 | 3 |
SpindlePole | 519 | 495 | 371 | 231 | 326 | 357 | 280 | 468 | 354 | 405 | 250 | 293 | 177 | 243 | 266 | 435 | 313 | 292 | 554 | 505 | 365 | 192 | 270 | 296 |
Vac/Vac Membrane | 0 | 5 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 3 | 10 | 4 | 4 | 0 | 1 | 0 | 1 | 2 | 3 |
Unique Cell Count | 586 | 579 | 398 | 254 | 371 | 410 | 341 | 541 | 408 | 472 | 319 | 348 | 214 | 332 | 410 | 539 | 391 | 353 | 604 | 603 | 411 | 222 | 329 | 350 |
Labelled Cell Count | 926 | 924 | 554 | 340 | 522 | 594 | 490 | 758 | 599 | 686 | 468 | 517 | 332 | 497 | 650 | 876 | 648 | 591 | 604 | 603 | 411 | 222 | 329 | 350 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 7.7 | 7.1 | 6.5 | 7.0 | 6.1 | 6.3 | 6.5 | 5.6 | 5.9 | 5.3 | 5.7 | 7.1 | 7.1 | 6.9 | 7.5 | 7.7 | 7.1 | 6.7 | 6.8 | 6.8 |
Std Deviation (1e-4) | 1.0 | 1.3 | 1.1 | 1.3 | 1.6 | 1.6 | 1.5 | 1.4 | 1.4 | 1.6 | 1.8 | 1.3 | 1.3 | 1.6 | 1.7 | 1.7 | 1.7 | 1.6 | 1.4 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.03 | -0.23 | -0.18 | -0.12 | -0.34 | -0.27 | -0.43 | -0.32 | 0.01 | -0.0 | -0.05 | 0.07 | 0.11 | -0.01 | -0.09 | -0.06 | -0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 3.0 | 2.5 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -1.0 | -0.5 | 0.1 | -0.4 | -0.9 | -0.1 | 0.4 | 0.5 | -1.2 | 0 | -1.8 | -2.2 | -2.8 | -1.8 | -2.3 |
Bud Site | 0 | 0 | 2.1 | 3.2 | 2.1 | 3.1 | 3.5 | 3.1 | 0 | 0 | 0 | 0 | 1.8 | 0 | 2.3 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.2 | 0.6 | 0.1 | 0.6 | 0.1 | -0.5 | -0.6 | -0.2 | -0.6 | 2.7 | 5.3 | 7.4 | 2.4 | 2.8 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.4 | 0.4 | 0.8 | 0.1 | -0.4 | 0.6 | -1.1 | -0.1 | 1.6 | 0.7 | 5.4 | 7.0 | 4.0 | 5.0 | 4.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 6.1 | 0 | 0 | 0 | 0 | 4.8 | 3.7 | 3.5 |
Nucleus | 0 | 2.9 | 2.0 | 3.1 | 2.5 | 1.6 | 1.5 | 2.5 | 2.1 | 0 | 0 | 2.5 | 3.6 | 4.6 | 4.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.0 | 0.8 | 1.4 | 1.5 | 0.3 | 0.7 | 1.5 | 0.3 | 2.8 | 5.2 | 3.0 | 2.8 | 3.5 | 3.6 | 3.9 |
Peroxisomes | 0 | 0 | 1.5 | 0 | 0.5 | 2.4 | 0.5 | 3.0 | 1.3 | 0 | 1.2 | 4.2 | 2.2 | 2.1 | 0.5 |
SpindlePole | -1.1 | -2.5 | -2.9 | -4.6 | -3.3 | -3.0 | -3.5 | -5.8 | -3.9 | -4.1 | -7.4 | -9.9 | -5.5 | -5.4 | -4.5 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5416 | 2.1499 | 2.4069 | 2.1523 | 2.3814 | 2.2296 | 2.1193 | 1.9037 | 2.1271 | 2.4042 | 1.9666 | 2.2316 | 2.4232 | 2.3341 | 2.1365 | 2.4415 | 2.2978 | 2.3986 |
Actin | 0.0112 | 0.0003 | 0.002 | 0.0001 | 0.0154 | 0.0014 | 0.0111 | 0.0014 | 0.0021 | 0.0032 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0006 | 0.0027 | 0.0001 | 0 | 0.0003 | 0.0004 | 0.0004 | 0.0005 | 0.0004 | 0.0055 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.003 | 0.0021 | 0.0004 | 0.0008 | 0.0003 | 0.0003 | 0.0013 | 0.0005 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0013 | 0.0002 |
Bud Periphery | 0.0007 | 0.0005 | 0.0001 | 0 | 0.0004 | 0.001 | 0.0003 | 0.0002 | 0.0003 | 0.0109 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0088 | 0.0107 | 0.0008 | 0.0001 | 0.0008 | 0.0003 | 0.0038 | 0.001 | 0.0059 | 0.0161 | 0 | 0 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0003 | 0 |
Cell Periphery | 0.0003 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0029 | 0.0058 | 0.0006 | 0.0004 | 0.0009 | 0.0008 | 0.0093 | 0.0002 | 0.0102 | 0.0041 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0346 | 0.0151 | 0.0106 | 0.0125 | 0.0767 | 0.0049 | 0.0163 | 0.0027 | 0.0099 | 0.0219 | 0.0085 | 0.0037 | 0.0157 | 0.0019 | 0.0018 | 0.0064 | 0.0319 | 0.0009 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0008 | 0.0001 | 0.0009 | 0.0001 | 0.0013 | 0.0008 | 0.0012 | 0.0001 | 0.0009 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0138 | 0.0013 | 0.0054 | 0.0262 | 0.0257 | 0.0081 | 0.017 | 0.0011 | 0.0105 | 0.0527 | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0021 | 0 |
Golgi | 0.0069 | 0.0005 | 0.0016 | 0.0061 | 0.0081 | 0.0024 | 0.0043 | 0.002 | 0.0024 | 0.0199 | 0.0019 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0023 | 0 |
Lipid Particles | 0.0469 | 0.0012 | 0.0123 | 0.0034 | 0.0546 | 0.0073 | 0.0172 | 0.0005 | 0.0078 | 0.043 | 0.0739 | 0.0017 | 0.0004 | 0.0001 | 0.0005 | 0.0006 | 0.0264 | 0.0002 |
Mitochondria | 0.0283 | 0.0005 | 0.0011 | 0.0016 | 0.0084 | 0.0071 | 0.0032 | 0.0029 | 0.0015 | 0.0264 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
None | 0.0102 | 0.0016 | 0.0004 | 0.0001 | 0.0003 | 0.0003 | 0.0052 | 0.0001 | 0.0011 | 0.0012 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.0056 | 0.0013 | 0.0022 | 0.0003 | 0.0006 | 0.0037 | 0.0051 | 0.0003 | 0.0028 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0007 | 0 |
Nucleolus | 0.0464 | 0.1003 | 0.068 | 0.0474 | 0.0109 | 0.0331 | 0.0525 | 0.0768 | 0.0405 | 0.0015 | 0.0025 | 0.0093 | 0.0296 | 0.0519 | 0.0378 | 0.0028 | 0.0235 | 0.0152 |
Nucleus | 0.0317 | 0.1055 | 0.0362 | 0.0158 | 0.0008 | 0.0154 | 0.0319 | 0.0435 | 0.0191 | 0.001 | 0.0003 | 0.0014 | 0.0034 | 0.0238 | 0.0134 | 0.0008 | 0.0009 | 0.0087 |
Peroxisomes | 0.0102 | 0.0005 | 0.0106 | 0.0005 | 0.003 | 0.0014 | 0.0099 | 0.0001 | 0.0019 | 0.006 | 0.0017 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0057 | 0 |
Punctate Nuclear | 0.7337 | 0.7487 | 0.8461 | 0.8838 | 0.79 | 0.909 | 0.8075 | 0.8652 | 0.8806 | 0.7804 | 0.9101 | 0.9832 | 0.9503 | 0.9207 | 0.9459 | 0.9885 | 0.9046 | 0.9745 |
Vacuole | 0.0021 | 0.0009 | 0.0003 | 0.0004 | 0.0009 | 0.001 | 0.0017 | 0.0003 | 0.0009 | 0.0024 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Vacuole Periphery | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0013 | 0.0007 | 0.0003 | 0.0003 | 0.0017 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.6164 | 14.254 | 6.6988 | 10.5291 | 15.1606 | 13.8115 | 16.4539 | 13.9424 | 11.5937 | 19.1303 |
Translational Efficiency | 1.3899 | 1.3498 | 1.2965 | 0.8364 | 1.145 | 1.3047 | 1.0178 | 0.9459 | 1.0088 | 0.923 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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