Standard name | |
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Human Ortholog | |
Description | Transcriptional activator involved in maintenance of ion homeostasis; also involved in protection against DNA damage caused by bleomycin and other oxidants; contains a C-terminal leucine-rich repeat |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 1 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 2 | 1 | 2 | 2 | 1 | 0 | 1 | 1 | 2 |
Bud | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 1 | 5 | 0 | 0 | 0 | 2 |
Bud Site | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 5 | – | – | 11 | – | – | – | – | – | – | – | – | – | – | 82 | 63 | 95 | 26 | 13 | 4 | 15 | 23 | 39 |
Cytoplasm | – | 18 | – | – | 37 | – | – | – | – | – | – | – | – | – | – | 104 | 63 | 99 | 9 | 32 | 2 | 10 | 13 | 34 |
Endoplasmic Reticulum | – | 0 | – | – | 1 | – | – | – | – | – | – | – | – | – | – | 7 | 1 | 6 | 4 | 1 | 1 | 0 | 0 | 3 |
Endosome | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 3 | 5 | 4 | 2 | 1 | 0 | 0 | 2 | 0 |
Nucleus | – | 1 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | – | – | 1 | – | – | – | – | – | – | – | – | – | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Unique Cell Count | 21 | 42 | 145 | 99 | 156 | 53 | 56 | 11 | 31 | 47 | 88 | |||||||||||||
Labelled Cell Count | 25 | 50 | 204 | 136 | 210 | 53 | 56 | 11 | 31 | 47 | 88 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 6.7 | – | – | 7.3 | – | – | – | – | – | – | – | – | – | – | 8.6 | 9.2 | 9.1 | – | – | 8.2 |
Std Deviation (1e-4) | – | 0.7 | – | – | 2.4 | – | – | – | – | – | – | – | – | – | – | 1.6 | 1.4 | 1.4 | – | – | 2.2 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0165 | 0.0123 | 0.0132 | 0.0158 | 0.0013 | 0.0027 | 0.0431 | 0.0015 | 0.0107 | 0.0034 | 0.0201 | 0.0057 | 0.0171 | 0.001 | 0.0326 | 0.1725 | 0.0159 | 0.0005 |
Bud | 0.0009 | 0.0011 | 0.0017 | 0.0013 | 0.0006 | 0.0027 | 0.0012 | 0.0002 | 0.0058 | 0.0034 | 0.0013 | 0.0006 | 0.0002 | 0.0005 | 0.001 | 0.0004 | 0.0007 | 0 |
Bud Neck | 0.0048 | 0.0021 | 0.0049 | 0.0042 | 0.0023 | 0.0088 | 0.0034 | 0.0008 | 0.0068 | 0.0115 | 0.0022 | 0.0027 | 0.0007 | 0.0005 | 0.0009 | 0.0009 | 0.0025 | 0.0022 |
Bud Periphery | 0.0022 | 0.003 | 0.0044 | 0.0096 | 0.0011 | 0.0061 | 0.0028 | 0.0004 | 0.0073 | 0.0056 | 0.004 | 0.0014 | 0.0004 | 0.0011 | 0.0021 | 0.0009 | 0.0007 | 0.0005 |
Bud Site | 0.0143 | 0.0808 | 0.0462 | 0.0027 | 0.0027 | 0.0021 | 0.0097 | 0.0197 | 0.0173 | 0.0354 | 0.0015 | 0.0005 | 0.0015 | 0.0299 | 0.0156 | 0.0006 | 0.0018 | 0.0001 |
Cell Periphery | 0.0321 | 0.0275 | 0.025 | 0.0303 | 0.0146 | 0.0148 | 0.0185 | 0.0193 | 0.0128 | 0.0126 | 0.0034 | 0.0123 | 0.0312 | 0.0284 | 0.0158 | 0.0009 | 0.0003 | 0.0681 |
Cytoplasm | 0.5509 | 0.737 | 0.6553 | 0.5979 | 0.8059 | 0.7492 | 0.6031 | 0.8561 | 0.7737 | 0.6622 | 0.4821 | 0.8693 | 0.7283 | 0.793 | 0.7706 | 0.2989 | 0.0026 | 0.8989 |
Cytoplasmic Foci | 0.0477 | 0.0028 | 0.0243 | 0.0389 | 0.0073 | 0.0068 | 0.0254 | 0.0013 | 0.0113 | 0.046 | 0.0198 | 0.0032 | 0.0123 | 0.0012 | 0.0142 | 0.1041 | 0.2154 | 0.0007 |
Eisosomes | 0.0007 | 0.0006 | 0.0005 | 0.0003 | 0.0001 | 0.0003 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0006 | 0.0016 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0053 | 0.0026 | 0.0024 | 0.0061 | 0.003 | 0.0026 | 0.0063 | 0.0027 | 0.0028 | 0.0055 | 0.0043 | 0.0047 | 0.0311 | 0.0057 | 0.0072 | 0.0131 | 0.0009 | 0.0057 |
Endosome | 0.0522 | 0.0004 | 0.0181 | 0.0301 | 0.0288 | 0.0157 | 0.037 | 0.0001 | 0.0031 | 0.0641 | 0.0619 | 0.0119 | 0.0154 | 0.0004 | 0.0113 | 0.0461 | 0.1988 | 0.0001 |
Golgi | 0.0213 | 0.0005 | 0.0103 | 0.0193 | 0.0114 | 0.0052 | 0.0285 | 0.0002 | 0.0058 | 0.0139 | 0.0552 | 0.0108 | 0.0089 | 0.0002 | 0.0392 | 0.015 | 0.4092 | 0.0001 |
Lipid Particles | 0.0201 | 0.0003 | 0.0073 | 0.019 | 0.009 | 0.004 | 0.0308 | 0.0003 | 0.006 | 0.0296 | 0.0491 | 0.0054 | 0.0223 | 0.0005 | 0.0026 | 0.1677 | 0.0963 | 0.0002 |
Mitochondria | 0.0196 | 0.0003 | 0.0103 | 0.0073 | 0.0219 | 0.0025 | 0.0298 | 0 | 0.0032 | 0.0601 | 0.1188 | 0.0238 | 0.007 | 0.0009 | 0.0044 | 0.0124 | 0.0163 | 0 |
None | 0.1438 | 0.1251 | 0.1431 | 0.1662 | 0.0826 | 0.1608 | 0.1219 | 0.0957 | 0.0952 | 0.0052 | 0.0722 | 0.0422 | 0.0788 | 0.1098 | 0.0534 | 0.0231 | 0.0002 | 0.0221 |
Nuclear Periphery | 0.0104 | 0.0002 | 0.0006 | 0.0022 | 0.0003 | 0.0011 | 0.0056 | 0.0001 | 0.0004 | 0.0018 | 0.0186 | 0.0013 | 0.029 | 0.0229 | 0.0011 | 0.1049 | 0.0003 | 0.0001 |
Nucleolus | 0.0015 | 0.0002 | 0.0012 | 0.0006 | 0.0003 | 0.0007 | 0.0012 | 0.0001 | 0.0008 | 0.0022 | 0.0011 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0 |
Nucleus | 0.0097 | 0.0007 | 0.0067 | 0.0022 | 0.0006 | 0.0027 | 0.0017 | 0.0003 | 0.0013 | 0.0052 | 0.0036 | 0.0005 | 0.0018 | 0.0009 | 0.0008 | 0.0032 | 0.0002 | 0.0001 |
Peroxisomes | 0.0265 | 0.0012 | 0.0163 | 0.037 | 0.0006 | 0.0022 | 0.0141 | 0 | 0.0288 | 0.013 | 0.0181 | 0.0011 | 0.0038 | 0 | 0.0228 | 0.0221 | 0.0328 | 0 |
Punctate Nuclear | 0.0073 | 0.0002 | 0.0011 | 0.0058 | 0.0007 | 0.0029 | 0.0036 | 0.0001 | 0.0053 | 0.0051 | 0.0274 | 0.0003 | 0.0021 | 0.0001 | 0.0006 | 0.0042 | 0.0011 | 0 |
Vacuole | 0.0101 | 0.0012 | 0.0053 | 0.0026 | 0.0033 | 0.0052 | 0.0099 | 0.0008 | 0.0013 | 0.0108 | 0.0323 | 0.0017 | 0.0024 | 0.0009 | 0.0027 | 0.0015 | 0.0025 | 0.0004 |
Vacuole Periphery | 0.0019 | 0 | 0.0018 | 0.0004 | 0.0017 | 0.0009 | 0.0015 | 0 | 0.0001 | 0.0032 | 0.0027 | 0.0005 | 0.0047 | 0.0019 | 0.0002 | 0.0051 | 0.0012 | 0 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.4891 | 24.6357 | 27.4211 | 25.3303 | 24.0497 | 25.4246 | 28.0228 | 23.9248 | 45.735 | 22.4281 |
Translational Efficiency | 1.7218 | 1.1413 | 1.1543 | 1.8835 | 1.4355 | 1.4856 | 1.22 | 2.286 | 1.051 | 1.4715 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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