Standard name | |
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Human Ortholog | |
Description | Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 4 | 4 | 1 | 0 | 0 | – | 0 | 0 | 1 | 2 | 4 | 3 | 5 | 11 | 3 | 6 | 1 | 19 | 2 | 3 | 1 | 6 |
Bud | 11 | 10 | 1 | 4 | 9 | 10 | 8 | – | 7 | 8 | 7 | 12 | 0 | 3 | 0 | 16 | 5 | 10 | 3 | 3 | 0 | 1 | 1 | 1 |
Bud Neck | 2 | 0 | 2 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 2 | 1 |
Bud Site | 2 | 1 | 0 | 0 | 0 | 0 | 4 | – | 3 | 2 | 7 | 5 | 0 | 0 | 0 | 1 | 2 | 8 | – | – | – | – | – | – |
Cell Periphery | 5 | 17 | 3 | 4 | 5 | 6 | 3 | – | 1 | 3 | 4 | 1 | 1 | 3 | 7 | 9 | 14 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 15 | 17 | 27 | 17 | 15 | 11 | 30 | – | 10 | 11 | 20 | 5 | 2 | 29 | 55 | 19 | 18 | 15 | 1 | 3 | 2 | 2 | 4 | 1 |
Endoplasmic Reticulum | 4 | 10 | 6 | 2 | 6 | 7 | 3 | – | 4 | 1 | 3 | 6 | 6 | 4 | 10 | 6 | 5 | 3 | 2 | 4 | 0 | 1 | 1 | 2 |
Endosome | 18 | 44 | 15 | 10 | 30 | 15 | 15 | – | 1 | 10 | 0 | 0 | 7 | 15 | 18 | 20 | 26 | 22 | 31 | 40 | 24 | 13 | 10 | 18 |
Golgi | 4 | 22 | 3 | 7 | 16 | 4 | 7 | – | 0 | 2 | 0 | 0 | 17 | 16 | 23 | 56 | 73 | 59 | 16 | 46 | 3 | 3 | 9 | 8 |
Mitochondria | 330 | 322 | 40 | 117 | 156 | 234 | 271 | – | 229 | 225 | 296 | 276 | 23 | 52 | 23 | 20 | 26 | 22 | 274 | 352 | 169 | 122 | 156 | 184 |
Nucleus | 3 | 0 | 2 | 2 | 2 | 8 | 10 | – | 5 | 10 | 3 | 8 | 0 | 1 | 2 | 4 | 6 | 2 | 1 | 1 | 0 | 0 | 1 | 2 |
Nuclear Periphery | 3 | 6 | 1 | 1 | 2 | 5 | 7 | – | 3 | 4 | 12 | 8 | 1 | 0 | 0 | 0 | 4 | 0 | 3 | 3 | 0 | 0 | 0 | 3 |
Nucleolus | 3 | 1 | 0 | 0 | 3 | 4 | 5 | – | 8 | 2 | 4 | 4 | 0 | 1 | 0 | 14 | 8 | 12 | 1 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 4 | 28 | 5 | 14 | 3 | 6 | 4 | – | 0 | 1 | 2 | 1 | 1 | 11 | 3 | 40 | 36 | 55 | 10 | 16 | 11 | 8 | 3 | 9 |
SpindlePole | 9 | 11 | 15 | 12 | 7 | 7 | 14 | – | 8 | 13 | 8 | 9 | 2 | 4 | 3 | 48 | 42 | 51 | 4 | 3 | 1 | 2 | 3 | 4 |
Vac/Vac Membrane | 46 | 84 | 25 | 9 | 43 | 29 | 28 | – | 12 | 19 | 22 | 13 | 12 | 19 | 19 | 36 | 31 | 52 | 25 | 24 | 10 | 8 | 15 | 15 |
Unique Cell Count | 366 | 438 | 129 | 160 | 243 | 289 | 334 | 252 | 260 | 324 | 299 | 58 | 126 | 136 | 235 | 241 | 246 | 385 | 530 | 233 | 177 | 218 | 266 | |
Labelled Cell Count | 459 | 575 | 149 | 204 | 298 | 346 | 409 | 291 | 312 | 389 | 351 | 76 | 161 | 168 | 300 | 299 | 329 | 385 | 530 | 233 | 177 | 218 | 266 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 6.0 | 5.9 | 6.2 | 6.8 | 5.9 | 6.1 | – | 5.1 | 5.0 | 4.5 | 4.6 | 6.9 | 6.9 | 6.5 | 10.8 | 11.4 | 11.5 | 5.2 | 5.9 | 6.0 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.1 | 1.7 | 1.4 | 1.4 | 1.7 | – | 1.5 | 1.2 | 1.4 | 0.9 | 0.9 | 1.2 | 1.1 | 2.6 | 3.0 | 3.0 | 1.1 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.22 | 0.0 | 0.05 | – | -0.2 | -0.24 | -0.37 | -0.34 | 0.23 | 0.24 | 0.15 | 0.88 | 0.96 | 0.97 | -0.16 | 0.02 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 1.2 |
Cytoplasm | -2.4 | -4.3 | -5.6 | -3.5 | – | -5.3 | -5.2 | -4.6 | -7.0 | -3.1 | 0.4 | 3.4 | -3.5 | -3.8 | -4.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 0 | 0 | 0 |
Endosome | -1.6 | 0.2 | -2.4 | -2.8 | – | -5.2 | -2.9 | 0 | 0 | 0.1 | 0.1 | 0.4 | -1.0 | -0.2 | -0.8 |
Golgi | 0 | 1.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | 5.5 | 3.2 | 4.0 | 5.3 | 6.3 | 5.4 |
Mitochondria | 7.1 | 6.1 | 9.9 | 10.3 | – | 12.1 | 11.1 | 0 | 0 | 1.2 | 1.7 | -2.7 | -5.5 | -4.8 | -5.5 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.7 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 3.6 | 3.2 | 4.6 |
SpindlePole | -1.2 | -3.4 | -3.9 | -3.0 | – | -3.3 | -2.4 | 0 | 0 | -1.8 | -2.6 | -3.0 | 2.1 | 1.5 | 2.2 |
Vacuole | -3.6 | -0.4 | -2.6 | -3.3 | – | -4.6 | -3.5 | -4.0 | -5.0 | 0.2 | -0.9 | -1.2 | -1.0 | -1.7 | 0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7921 | 1.7791 | 1.5301 | 2.0488 | 0.8738 | 1.6873 | 3.1037 | 3.5995 | 3.3215 | 3.2982 | 3.0584 | 3.2388 | 3.019 | 3.6212 | 3.0739 | 3.9151 | 3.1358 | 3.3118 |
Actin | 0.0406 | 0.0253 | 0.009 | 0.0037 | 0.0044 | 0.0117 | 0.0109 | 0.0129 | 0.019 | 0.0026 | 0.0045 | 0.0085 | 0.0184 | 0.0059 | 0.0031 | 0.0369 | 0.0031 | 0.0018 |
Bud | 0.001 | 0.0025 | 0.0011 | 0.0014 | 0.0093 | 0.0014 | 0.0017 | 0.0016 | 0.0024 | 0.0022 | 0.0003 | 0.0005 | 0.0006 | 0.0017 | 0.001 | 0.0006 | 0.0004 | 0.0001 |
Bud Neck | 0.0009 | 0.0016 | 0.0006 | 0.0008 | 0.0007 | 0.0007 | 0.0007 | 0.0018 | 0.0015 | 0.0007 | 0.0003 | 0.0006 | 0.0007 | 0.0008 | 0.0003 | 0.0002 | 0 | 0.0001 |
Bud Periphery | 0.0019 | 0.0025 | 0.0017 | 0.009 | 0.0245 | 0.0066 | 0.0015 | 0.0027 | 0.0067 | 0.0084 | 0.0006 | 0.0013 | 0.001 | 0.0026 | 0.0017 | 0.0008 | 0.0004 | 0.0002 |
Bud Site | 0.0061 | 0.0218 | 0.0024 | 0.0012 | 0.003 | 0.0003 | 0.0035 | 0.0327 | 0.0042 | 0.0009 | 0.0005 | 0.0023 | 0.0021 | 0.0073 | 0.0016 | 0.0007 | 0.0008 | 0.0001 |
Cell Periphery | 0.0009 | 0.0009 | 0.0003 | 0.0132 | 0.0013 | 0.0003 | 0.0007 | 0.0034 | 0.0007 | 0.0004 | 0.0001 | 0.0004 | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.0019 | 0.005 | 0.0036 | 0.003 | 0.0059 | 0.0007 | 0.0032 | 0.0025 | 0.002 | 0.0006 | 0.0015 | 0.0006 | 0.0028 | 0.0015 | 0.0007 | 0.0017 | 0.0001 | 0.0006 |
Cytoplasmic Foci | 0.0267 | 0.0175 | 0.0304 | 0.0147 | 0.0261 | 0.0029 | 0.0295 | 0.0179 | 0.0165 | 0.0108 | 0.0122 | 0.0084 | 0.018 | 0.0244 | 0.012 | 0.0166 | 0.0105 | 0.0078 |
Eisosomes | 0.0007 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0026 | 0.0028 | 0.0016 | 0.0008 | 0.0011 | 0.0008 | 0.002 | 0.002 | 0.0022 | 0.0006 | 0.0009 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0002 |
Endosome | 0.054 | 0.0505 | 0.0501 | 0.0962 | 0.099 | 0.0198 | 0.0597 | 0.0529 | 0.0258 | 0.0415 | 0.0514 | 0.0353 | 0.0473 | 0.0521 | 0.0318 | 0.0391 | 0.0023 | 0.0191 |
Golgi | 0.0287 | 0.031 | 0.0767 | 0.1041 | 0.0725 | 0.0358 | 0.0471 | 0.0545 | 0.0272 | 0.0543 | 0.0759 | 0.0467 | 0.0404 | 0.0484 | 0.0511 | 0.076 | 0.0079 | 0.0481 |
Lipid Particles | 0.0366 | 0.0101 | 0.0136 | 0.0103 | 0.0041 | 0.0011 | 0.0438 | 0.0075 | 0.0063 | 0.0177 | 0.0127 | 0.0088 | 0.0328 | 0.0183 | 0.004 | 0.002 | 0.0022 | 0.0024 |
Mitochondria | 0.7117 | 0.7642 | 0.7469 | 0.7151 | 0.7078 | 0.9123 | 0.7309 | 0.765 | 0.8505 | 0.8388 | 0.7784 | 0.854 | 0.7746 | 0.7824 | 0.8726 | 0.814 | 0.9184 | 0.9123 |
None | 0.0031 | 0.0029 | 0.0045 | 0.0003 | 0.0005 | 0.0001 | 0.0048 | 0.0005 | 0.0067 | 0.0003 | 0.0016 | 0.0004 | 0.0092 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0058 | 0.0007 | 0.0006 | 0.0002 | 0.0005 | 0.0002 | 0.009 | 0.0017 | 0.0042 | 0.0007 | 0.0138 | 0.0007 | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Nucleolus | 0.0006 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0009 | 0.0008 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0021 | 0.0009 | 0.0001 | 0 | 0.0001 | 0 |
Nucleus | 0.0009 | 0.0005 | 0.0003 | 0.0003 | 0.0007 | 0.0001 | 0.0019 | 0.0005 | 0.0004 | 0.0005 | 0.0038 | 0.0004 | 0.0023 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 |
Peroxisomes | 0.0381 | 0.0352 | 0.0381 | 0.0093 | 0.0189 | 0.0022 | 0.0333 | 0.0213 | 0.0152 | 0.0052 | 0.0238 | 0.0237 | 0.0263 | 0.0416 | 0.0148 | 0.0057 | 0.0526 | 0.0047 |
Punctate Nuclear | 0.0058 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0066 | 0.0002 | 0.0004 | 0.0001 | 0.0123 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Vacuole | 0.013 | 0.0186 | 0.0099 | 0.0084 | 0.0115 | 0.0011 | 0.0036 | 0.012 | 0.0037 | 0.0071 | 0.0015 | 0.0023 | 0.0089 | 0.0072 | 0.002 | 0.0019 | 0.0003 | 0.0007 |
Vacuole Periphery | 0.0183 | 0.0053 | 0.0077 | 0.0072 | 0.0076 | 0.0013 | 0.0044 | 0.0051 | 0.004 | 0.0064 | 0.003 | 0.0036 | 0.0091 | 0.0031 | 0.002 | 0.003 | 0.0006 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.0681 | 18.7159 | 22.1457 | 23.5627 | 11.3715 | 29.9734 | 33.1163 | 36.9357 | 40.0017 | 24.0065 |
Translational Efficiency | 0.692 | 0.5561 | 0.6354 | 0.8971 | 0.6983 | 0.8155 | 0.65 | 0.7028 | 0.7307 | 0.5342 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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