Standard name | |
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Human Ortholog | |
Description | Subunit of a complex that associates with actin filaments; forms a complex with Tda2p that inhibits barbed end F-actin assembly; elevates actin monomer pools to increase endocytotic efficiency and to regulate the distribution of actin between cables and patches; Aim21p/Tda2p forms a larger complex with actin capping proteins Cap1p and Cap2p; involved in mitochondrial migration along actin filaments; recruited to cortical actin patches by SH3 domain-containing proteins Bbc1p and Abp1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 191 | 138 | 67 | 80 | 74 | 120 | 107 | 119 | 35 | 77 | 29 | 76 | 143 | 172 | 158 | – | – | – | 240 | 179 | 87 | 137 | 240 | 296 |
Bud | 48 | 33 | 11 | 24 | 22 | 28 | 36 | 37 | 12 | 35 | 16 | 19 | 31 | 34 | 32 | – | – | – | 18 | 10 | 5 | 25 | 45 | 39 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 7 | 10 | 7 | 21 | 26 | 26 |
Bud Site | 16 | 2 | 1 | 4 | 4 | 6 | 5 | 5 | 0 | 3 | 0 | 4 | 5 | 4 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 21 | 20 | 9 | 9 | 13 | 23 | 14 | 16 | 1 | 18 | 4 | 5 | 22 | 47 | 42 | – | – | – | 0 | 1 | 1 | 0 | 1 | 2 |
Cytoplasm | 50 | 35 | 22 | 15 | 43 | 23 | 38 | 54 | 13 | 28 | 17 | 15 | 53 | 53 | 93 | – | – | – | 12 | 17 | 5 | 12 | 13 | 31 |
Endoplasmic Reticulum | 30 | 26 | 11 | 12 | 11 | 35 | 54 | 42 | 20 | 25 | 17 | 29 | 24 | 33 | 47 | – | – | – | 2 | 2 | 3 | 1 | 4 | 7 |
Endosome | 1 | 9 | 5 | 6 | 2 | 2 | 2 | 9 | 5 | 8 | 10 | 6 | 6 | 1 | 5 | – | – | – | 7 | 13 | 13 | 10 | 20 | 12 |
Golgi | 13 | 34 | 13 | 10 | 10 | 17 | 30 | 17 | 9 | 13 | 5 | 19 | 24 | 44 | 35 | – | – | – | 5 | 10 | 3 | 2 | 6 | 3 |
Mitochondria | 66 | 10 | 4 | 10 | 4 | 42 | 39 | 45 | 79 | 84 | 92 | 76 | 2 | 3 | 1 | – | – | – | 9 | 6 | 11 | 5 | 10 | 8 |
Nucleus | 1 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | – | – | – | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 7 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 62 | 18 | 10 | 32 | 11 | 23 | 11 | 6 | 4 | 14 | 7 | 5 | 32 | 36 | 27 | – | – | – | 17 | 4 | 7 | 4 | 2 | 10 |
SpindlePole | 4 | 5 | 2 | 9 | 5 | 2 | 4 | 7 | 0 | 3 | 1 | 1 | 9 | 10 | 9 | – | – | – | 1 | 0 | 0 | 2 | 2 | 1 |
Vac/Vac Membrane | 1 | 1 | 1 | 3 | 1 | 2 | 4 | 15 | 7 | 7 | 2 | 3 | 1 | 2 | 1 | – | – | – | 2 | 2 | 1 | 2 | 2 | 5 |
Unique Cell Count | 295 | 213 | 108 | 129 | 138 | 216 | 221 | 274 | 133 | 209 | 139 | 166 | 227 | 282 | 310 | 329 | 263 | 153 | 230 | 382 | 450 | |||
Labelled Cell Count | 507 | 331 | 159 | 216 | 200 | 324 | 347 | 379 | 187 | 317 | 202 | 259 | 352 | 440 | 452 | 329 | 263 | 153 | 230 | 382 | 450 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 9.9 | 8.9 | 9.1 | 9.6 | 8.1 | 8.4 | 8.6 | 7.1 | 7.5 | 7.0 | 7.3 | 10.1 | 10.3 | 9.6 | – | – | – | 9.3 | 9.2 | 9.5 |
Std Deviation (1e-4) | 1.1 | 1.8 | 1.5 | 2.0 | 1.9 | 1.6 | 1.6 | 1.6 | 1.6 | 1.5 | 2.1 | 1.4 | 1.6 | 1.7 | 1.6 | – | – | – | 1.5 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.11 | -0.12 | -0.08 | -0.05 | -0.31 | -0.23 | -0.35 | -0.29 | 0.19 | 0.22 | 0.12 | – | – | – | 0.07 | 0.06 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -1.3 | -1.1 | -2.3 | -3.3 | -5.6 | -4.3 | -6.6 | -2.6 | 0.2 | -0.2 | -2.0 | – | – | – |
Bud | 1.8 | 1.3 | 0.7 | 1.5 | 0.9 | -0.3 | 1.6 | 0.3 | 0.3 | 0.9 | 0.5 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | -0.4 | 0.3 | 0.7 | -0.7 | -0.9 | 0 | 0.1 | -1.9 | -2.0 | 0.4 | 2.1 | 1.4 | – | – | – |
Cytoplasm | -1.8 | 1.9 | -2.4 | -0.7 | -0.1 | -2.3 | -1.6 | -1.7 | -2.7 | 0.6 | -0.4 | 1.9 | – | – | – |
Endoplasmic Reticulum | -0.2 | -0.6 | 1.5 | 3.0 | 1.3 | 1.1 | 0.5 | 0.5 | 1.7 | 0.1 | 0.4 | 1.3 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | -1.1 | -1.3 | -1.2 | 0.4 | -1.9 | -1.4 | -1.8 | -2.5 | -0.1 | -0.4 | 0.9 | -0.2 | – | – | – |
Mitochondria | 1.3 | 0 | 3.8 | 3.5 | 3.3 | 9.0 | 6.9 | 10.0 | 7.5 | 0 | 0 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 3.1 | -0.4 | 0.4 | -1.5 | 0 | -2.1 | -0.8 | -1.3 | -2.2 | 1.2 | 1.0 | -0.2 | – | – | – |
SpindlePole | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |