Standard name
Human Ortholog
Description U2B component of U2 snRNP; involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.06
Bud Neck – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – – 0 0.05 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – – 0.5 0.34 0.39 0.33 – 0.38 0.25 0.22 0.21 0.21 0.56 0.66 0.65 0.11 0.18 0.13 0.36 0.08 0.25 0.23 0.31 0.28
Endoplasmic Reticulum – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Endosome – – 0 0 0.16 0 – 0.08 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0
Golgi – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – – 0.41 0.51 0.12 0.49 – 0.37 0.75 0.57 0.69 0.79 0 0 0 0.09 0.09 0.07 0 0 0.08 0.07 0.09 0.05
Nucleus – – 0.19 0.2 0.27 0.31 – 0.26 0.15 0.33 0.17 0.17 0.32 0.18 0.22 0.69 0.67 0.73 0.33 0.4 0.23 0.32 0.2 0.24
Nuclear Periphery – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – – 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0.08 0 0.05
Vac/Vac Membrane – – 0.09 0.07 0.14 0.09 – 0 0.11 0 0.07 0.06 0.12 0.13 0.09 0.14 0.12 0.09 0.13 0.15 0.19 0.17 0.24 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
Bud – – 0 0 0 0 – 1 0 1 1 0 0 0 1 1 0 0 2 1 2 1 2 6
Bud Neck – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 1
Bud Site – – 0 2 0 0 – 1 1 3 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm – – 16 14 19 15 – 28 14 13 12 18 19 55 83 18 37 20 23 2 19 13 26 26
Endoplasmic Reticulum – – 0 0 0 0 – 1 1 0 0 0 0 1 0 11 10 5 0 0 1 0 0 0
Endosome – – 0 0 8 0 – 6 0 1 0 2 0 2 3 5 1 2 0 1 3 0 2 2
Golgi – – 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 1 0 1 0 0 2 0
Mitochondria – – 13 21 6 22 – 27 41 33 40 66 1 3 3 15 18 11 2 1 6 4 7 5
Nucleus – – 6 8 13 14 – 19 8 19 10 14 11 15 28 111 138 115 21 12 17 19 16 23
Nuclear Periphery – – 0 0 0 0 – 1 1 0 1 0 0 0 0 2 1 0 0 0 0 0 0 0
Nucleolus – – 0 2 1 0 – 1 1 0 1 0 0 1 1 1 3 1 1 0 0 0 0 1
Peroxisomes – – 0 0 0 0 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – – 0 0 1 0 – 0 0 0 1 1 0 0 2 0 2 1 2 3 2 4 1 4
Vac/Vac Membrane – – 3 3 7 4 – 3 6 1 4 5 4 11 11 23 24 14 8 4 14 10 20 20
Unique Cell Count 32 41 49 45 73 55 58 58 84 34 83 128 161 205 157 66 31 76 60 85 95
Labelled Cell Count 38 50 55 55 89 73 72 70 107 35 89 132 189 235 170 66 31 76 60 85 95


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 3.5 3.8 5.0 3.3 – 4.5 3.4 4.5 3.9 3.4 4.4 4.5 4.6 6.9 6.7 6.9 4.3 4.3 4.3
Std Deviation (1e-4) – – 1.2 2.6 2.8 0.7 – 3.3 1.7 3.3 2.1 1.5 1.2 1.6 1.4 1.6 3.0 2.4 1.7 1.1 1.1
Intensity Change (Log2) – – – 0.13 0.51 -0.06 – 0.37 -0.04 0.36 0.15 -0.04 0.32 0.38 0.39 0.99 0.94 0.98 0.31 0.3 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 -1.0 -1.5 – -1.1 -2.3 -2.7 -2.9 -3.0 0.5 1.6 1.5 -5.3 -4.0 -4.9
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -2.9 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.1 0.8 1.2 – 0.8 -0.5 1.4 -0.2 -0.3 1.3 -0.1 0.4 5.3 5.2 5.9
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0433 0.0198 0.0043 0.0684 0.0024 0.0038 0.1033 0.0005 0.0512 0.009 0.0102 0.0188 – – – – – –
Bud 0.001 0.0003 0.0002 0.0014 0.0001 0.0005 0.001 0.0038 0.0008 0.0003 0.0007 0.0001 – – – – – –
Bud Neck 0.002 0.0025 0.0004 0.0093 0.0008 0.0023 0.0022 0.0006 0.0104 0.0008 0.0011 0.0053 – – – – – –
Bud Periphery 0.0008 0.0003 0.0001 0.002 0.0001 0.0025 0.0015 0.003 0.0009 0.0004 0.0021 0.0001 – – – – – –
Bud Site 0.0064 0.0164 0.001 0.0045 0.0002 0.0001 0.0063 0.0064 0.0108 0.0019 0.0017 0.0003 – – – – – –
Cell Periphery 0.0003 0.0003 0 0.0004 0.0001 0 0.001 0.0001 0.0004 0.0001 0.0004 0.0001 – – – – – –
Cytoplasm 0.1201 0.1604 0.0817 0.1299 0.0781 0.0972 0.0277 0.0822 0.1227 0.0841 0.0868 0.0748 – – – – – –
Cytoplasmic Foci 0.0724 0.0032 0.0172 0.0074 0.0023 0.0082 0.0877 0.0173 0.0416 0.0105 0.0195 0.0021 – – – – – –
Eisosomes 0.0017 0.0002 0.0001 0.0004 0.0001 0.0001 0.0016 0.0001 0.0003 0.0002 0.0006 0.0003 – – – – – –
Endoplasmic Reticulum 0.0236 0.0038 0.0054 0.0046 0.0111 0.0022 0.0164 0.0041 0.008 0.0027 0.0119 0.0093 – – – – – –
Endosome 0.0625 0.0021 0.0103 0.0219 0.0172 0.0036 0.1039 0.0041 0.0423 0.0285 0.1279 0.0032 – – – – – –
Golgi 0.035 0.0006 0.0058 0.0194 0.0005 0.0018 0.0476 0.001 0.0105 0.0094 0.0142 0.0005 – – – – – –
Lipid Particles 0.0657 0.0007 0.016 0.0285 0.001 0.0007 0.0597 0.0008 0.0108 0.0099 0.1348 0.0005 – – – – – –
Mitochondria 0.0076 0.0006 0.0021 0.0062 0.0014 0.0279 0.0492 0.001 0.0073 0.0178 0.1297 0.0005 – – – – – –
None 0.16 0.4413 0.3209 0.1876 0.4232 0.0629 0.0414 0.2789 0.2545 0.16 0.0333 0.0944 – – – – – –
Nuclear Periphery 0.0786 0.0085 0.0259 0.0103 0.0681 0.0124 0.086 0.0367 0.0578 0.0093 0.0874 0.0199 – – – – – –
Nucleolus 0.0032 0.0031 0.0041 0.0022 0.0037 0.0047 0.0067 0.0066 0.002 0.0026 0.0112 0.0073 – – – – – –
Nucleus 0.2503 0.3072 0.4289 0.4805 0.3659 0.6957 0.2691 0.5228 0.3124 0.6254 0.2154 0.7308 – – – – – –
Peroxisomes 0.0301 0.0004 0.0186 0.0025 0.0001 0.0007 0.0282 0.0018 0.0242 0.0034 0.0131 0.0003 – – – – – –
Punctate Nuclear 0.03 0.0264 0.0552 0.0093 0.019 0.0713 0.0497 0.0241 0.0166 0.0174 0.0667 0.0295 – – – – – –
Vacuole 0.0033 0.0017 0.0015 0.0022 0.0029 0.0007 0.0049 0.0031 0.0113 0.0049 0.0142 0.0015 – – – – – –
Vacuole Periphery 0.0024 0.0002 0.0005 0.0012 0.002 0.0005 0.0048 0.0007 0.0033 0.0014 0.0173 0.0003 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.7777 14.6782 6.5351 13.5715 6.3867 9.8464 14.0991 11.0184 18.8232 15.1894
Translational Efficiency 2.4595 1.3041 2.6007 1.8317 4.0732 4.9322 1.8461 2.0893 1.307 1.9556

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
37 91 1593 115 75 693 2350 82 112 784 3943 197

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 697.81 823.42 854.37 860.56 597.88 755.68 867.97 952.23 630.89 763.54 862.48 898.72
Standard Deviation 89.79 146.83 112.84 121.83 118.34 102.08 132.76 149.56 119.37 110.38 125.27 141.48
Intensity Change Log 2 — 0.238794 0.292027 0.302442 — 0.337919 0.537789 0.671454 — 0.285382 0.410649 0.484491

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000105 0.001627 0.000384 0.000845 0.000064 0.000641 0.000603 0.001295 0.000077 0.000755 0.000514 0.001033
Bud Neck 0.001880 0.012613 0.010551 0.009804 0.003434 0.013422 0.033689 0.010042 0.002921 0.013328 0.024341 0.009903
Bud Site 0.000393 0.009169 0.008168 0.007392 0.001085 0.006597 0.015919 0.031016 0.000856 0.006895 0.012788 0.017225
Cell Periphery 0.000157 0.000321 0.000171 0.000230 0.000352 0.000227 0.000228 0.000731 0.000288 0.000238 0.000205 0.000438
Cytoplasm 0.170781* 0.193236* 0.090057 0.214406* 0.237920* 0.227978* 0.107406* 0.197955* 0.215741* 0.223946* 0.100397* 0.207559*
Cytoplasmic Foci 0.123169 0.122972 0.001667 0.014288 0.128604 0.078277 0.000924 0.012732 0.126808 0.083465 0.001224 0.013640
Eisosomes 0.000095 0.000197 0.000025 0.000055 0.000481 0.000079 0.000023 0.000055 0.000354 0.000092 0.000024 0.000055
Endoplasmic Reticulum 0.001250 0.004801 0.004553 0.007164 0.001773 0.001215 0.005257 0.007634 0.001600 0.001632 0.004973 0.007360
Endosome 0.001027 0.010192 0.001138 0.007862 0.002910 0.010511 0.001938 0.022835 0.002288 0.010474 0.001615 0.014094
Golgi 0.000146 0.000624 0.000087 0.006155 0.001274 0.004014 0.000802 0.027503 0.000901 0.003621 0.000513 0.015041
Lipid Particles 0.006150 0.011642 0.000590 0.001886 0.015990 0.005218 0.000414 0.001323 0.012739 0.005963 0.000485 0.001652
Mitochondria 0.000710 0.001839 0.001263 0.011037 0.001008 0.012722 0.007972 0.015547 0.000910 0.011459 0.005261 0.012914
Mitotic Spindle 0.000062 0.002975 0.008522 0.110306* 0.000469 0.004905 0.030741 0.142914* 0.000335 0.004681 0.021764 0.123879*
None 0.002486 0.003951 0.007615 0.007730 0.015589 0.003983 0.007733 0.013044 0.011260 0.003979 0.007685 0.009942
Nuclear Periphery 0.000861 0.001678 0.002327 0.003358 0.001514 0.001770 0.004051 0.001787 0.001298 0.001759 0.003355 0.002704
Nuclear Periphery Foci 0.000336 0.002044 0.000316 0.001541 0.000437 0.000723 0.000545 0.001535 0.000404 0.000876 0.000453 0.001539
Nucleolus 0.002022 0.002992 0.001385 0.001600 0.002274 0.003642 0.002190 0.001236 0.002191 0.003566 0.001865 0.001448
Nucleus 0.675944* 0.510066* 0.848033* 0.546431* 0.559481* 0.586159* 0.754897* 0.393979* 0.597956* 0.577326* 0.792525* 0.482974*
Peroxisomes 0.002057 0.031533 0.000777 0.000734 0.008978 0.007367 0.000284 0.001331 0.006692 0.010172 0.000483 0.000983
Vacuole 0.010247 0.074908 0.012128 0.045201 0.016205 0.029331 0.022851 0.093980 0.014237 0.034621 0.018519 0.065505
Vacuole Periphery 0.000121 0.000620 0.000242 0.001974 0.000157 0.001219 0.001533 0.021526 0.000145 0.001150 0.001012 0.010112

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.76 -3.87 -3.80 0.68 -2.34 -1.66 -7.83 -6.50 -3.21 -4.51 -2.11 -8.91 -7.27 -2.32 -4.60
Bud Neck -3.00 -9.64 -4.54 0.72 0.68 -4.78 -15.07 -4.38 -0.55 5.62 -5.84 -16.90 -5.83 0.19 5.75
Bud Site -3.81 -5.72 -5.18 1.02 -0.53 -2.95 -9.00 -4.60 -2.45 -0.88 -3.80 -10.47 -6.50 -1.80 -0.50
Cell Periphery -1.63 -0.17 -0.95 1.01 -1.00 0.91 1.40 0.28 -0.83 -1.59 0.54 1.36 0.37 -0.29 -1.85
Cytoplasm 0.43 2.67 0.80 0.61 -3.35 0.09 5.27 3.33 4.74 -1.19 -0.15 5.84 2.91 4.71 -3.36
Cytoplasmic Foci 0.34 3.47 3.16 5.45 -2.93 2.33 6.22 5.96 13.34 -2.37 2.32 7.07 6.57 13.64 -3.72
Eisosomes -3.33 3.99 0.65 4.06 -5.00 1.48 1.68 1.34 -2.61 -6.84 1.43 1.80 1.40 -0.47 -8.44
Endoplasmic Reticulum -4.95 -12.96 -6.45 -2.02 -1.65 0.41 -5.95 -4.59 -4.88 -2.99 -0.60 -8.99 -6.73 -6.94 -3.40
Endosome -3.88 -0.82 -2.15 1.84 -2.02 -3.32 1.97 -1.68 1.07 -2.66 -4.24 1.79 -2.22 2.02 -3.28
Golgi -2.15 0.74 -1.19 -0.80 -1.21 -1.54 0.85 -1.12 -0.38 -1.38 -1.86 0.92 -1.42 -0.05 -1.80
Lipid Particles -1.35 2.15 1.49 2.97 -2.39 1.39 2.00 1.77 4.08 -4.11 1.28 2.29 1.98 5.08 -4.14
Mitochondria -1.86 -2.93 -1.15 -0.88 -0.88 -3.70 -6.00 -1.25 2.06 0.66 -3.77 -6.47 -1.56 1.85 -0.13
Mitotic Spindle -1.80 -6.39 -4.05 -3.36 -3.49 0.86 -0.23 -2.22 -3.28 -2.52 0.55 -0.53 -3.82 -4.85 -4.17
None -1.01 -4.45 -2.13 -1.61 -0.35 1.28 0.95 0.90 -1.11 -0.04 1.19 0.71 0.59 -1.86 -0.29
Nuclear Periphery -4.01 -11.09 -4.80 -3.09 -1.10 -1.03 -7.82 -3.72 -3.86 2.72 -2.01 -9.75 -5.23 -4.58 1.09
Nuclear Periphery Foci -2.47 -1.07 -3.28 0.38 -3.03 0.70 1.24 -1.37 -1.66 -1.84 -0.18 0.93 -2.31 -2.31 -3.00
Nucleolus -1.58 -0.10 -0.45 1.20 -0.43 -1.24 0.84 0.34 1.96 -0.63 -1.96 0.82 0.26 2.46 -0.63
Nucleus 2.87 -3.16 1.83 -1.44 8.67 -1.25 -6.44 0.29 1.61 6.69 0.18 -7.13 1.00 1.23 10.53
Peroxisomes -2.24 1.36 1.43 2.40 0.02 0.27 1.69 1.60 4.11 -2.54 -0.71 1.82 1.74 4.74 -1.39
Vacuole -5.73 -1.35 -5.32 0.71 -5.12 -0.16 0.01 -4.97 -5.59 -5.74 -1.89 -0.14 -6.31 -5.98 -7.45
Vacuole Periphery -3.14 -2.44 -1.25 -0.24 -1.05 -3.15 -3.43 -1.47 -1.09 -1.22 -3.41 -3.76 -1.76 -0.91 -1.37
Standard name
Human Ortholog
Description U2B component of U2 snRNP; involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
Localization
Cell Percentages nucleus (47%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Msl1

Msl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available