Standard name
Human Ortholog
Description Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to the vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 – 0 0 0.05 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.86 0.85 0.91 0.92 0.9 0.94 – 0.93 0.94 0.91 0.88 0.88 0.91 0.95 0.98 0.73 0.72 0.69 0.27 0.26 0.25 0.23 0.29 0.23
Endoplasmic Reticulum 0.06 0.08 0.05 0.05 0.05 0 – 0 0 0 0 0 0.07 0.05 0 0 0 0 0.15 0.14 0.16 0.26 0.15 0.14
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0.07 0.09 0.17 0.18 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.08 0 0 0 0 – 0 0 0 0 0 0 0 0 0.31 0.33 0.35 0.45 0.46 0.47 0.41 0.44 0.51
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 3 2 10 2 – 3 0 3 2 0 2 1 0 1 0 0 10 4 4 2 5 5
Bud 1 2 7 18 16 18 – 11 25 36 46 35 4 1 0 0 0 0 25 4 6 5 21 7
Bud Neck 0 0 1 0 0 0 – 0 0 0 1 0 0 0 1 0 0 0 12 4 3 0 3 3
Bud Site 0 0 0 0 0 0 – 0 1 1 1 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 10 15 18 – 6 2 4 6 5 4 5 4 1 2 2 1 0 1 0 1 1
Cytoplasm 557 169 268 347 374 575 – 890 702 684 512 617 425 504 628 137 145 141 185 56 79 39 134 125
Endoplasmic Reticulum 37 16 14 17 19 18 – 2 1 5 2 2 32 28 4 5 7 9 106 31 51 44 67 75
Endosome 3 0 0 1 6 5 – 13 6 10 4 3 5 0 1 7 4 9 9 3 2 2 6 9
Golgi 2 1 0 0 1 0 – 0 0 0 0 0 0 0 1 1 0 2 4 2 0 0 2 4
Mitochondria 11 1 7 8 1 5 – 24 55 70 99 129 0 2 1 4 1 4 13 5 14 6 12 16
Nucleus 0 1 1 0 0 3 – 2 1 0 0 1 2 1 1 3 0 1 0 0 0 0 1 1
Nuclear Periphery 1 1 0 0 0 1 – 13 1 0 0 1 0 1 0 0 0 0 0 1 0 0 0 1
Nucleolus 1 0 0 1 1 0 – 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 – 7 1 6 1 3 0 0 0 0 0 0 2 0 0 0 1 3
Vac/Vac Membrane 53 15 2 3 10 20 – 37 18 18 11 18 10 1 4 58 67 71 314 100 152 70 202 276
Unique Cell Count 646 198 294 376 414 614 962 750 752 582 703 467 531 639 187 201 203 692 220 322 173 465 538
Labelled Cell Count 672 207 305 407 453 665 1012 813 837 685 821 484 544 645 217 226 240 692 220 322 173 465 538


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.4 4.9 5.5 6.9 5.4 – 4.7 3.9 4.2 3.9 4.0 6.2 5.6 5.0 8.0 7.8 7.9 6.7 6.6 6.0
Std Deviation (1e-4) 2.9 2.5 2.6 2.8 2.2 1.7 – 1.3 0.8 0.6 0.6 1.3 2.6 1.6 1.3 2.6 3.2 3.0 3.6 3.1 1.9
Intensity Change (Log2) – – – 0.18 0.5 0.14 – -0.04 -0.33 -0.23 -0.32 -0.3 0.35 0.21 0.04 0.71 0.67 0.7 0.46 0.43 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 1.4 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 1.6 1.1 0.5 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.9 2.6 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 -0.4 1.4 – 0.8 1.4 -0.1 -1.4 -1.5 -0.1 2.1 5.2 -5.2 -5.6 -6.2
Endoplasmic Reticulum -0.1 -0.1 -1.4 – 0 0 0 0 0 1.2 0.3 -4.3 -1.1 -0.7 -0.2
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.2 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.4 – 2.7 1.8 1.8 0 1.9 0 0 0 9.8 10.3 10.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.381 1.0255 0.7976 0.5954 0.6488 0.6621 -0.3034 -0.021 -0.0193 0.4305 0.2038 -0.121 1.9721 2.543 2.3301 2.9566 2.1808 1.9677
Actin 0.0102 0.0004 0.01 0.0001 0.0108 0.0059 0.0266 0.0002 0.0072 0.0436 0.0605 0.0018 0.0161 0.0007 0.0121 0.0018 0.0288 0.004
Bud 0.0005 0.0003 0.0027 0.0002 0.0046 0.0019 0.0005 0.0038 0.0048 0.001 0.0013 0.0002 0.0002 0.0005 0.0007 0.0009 0.001 0.0007
Bud Neck 0.0151 0.0089 0.0012 0.0004 0.0026 0.0035 0.0093 0.0003 0.0018 0.0012 0.0015 0.0031 0.0003 0.001 0.0066 0.0017 0.0036 0.003
Bud Periphery 0.0005 0.0004 0.004 0.0004 0.0028 0.0041 0.0009 0.001 0.0036 0.0014 0.0028 0.0003 0.0002 0.0008 0.0017 0.0012 0.0019 0.0014
Bud Site 0.0029 0.0121 0.0063 0.0002 0.0029 0.0023 0.0105 0.0027 0.009 0.0025 0.0021 0.0004 0.0009 0.0107 0.0126 0.0491 0.0048 0.0011
Cell Periphery 0.0005 0.001 0.0007 0.0002 0.0012 0.0004 0.0011 0.0015 0.0008 0.0005 0.0005 0.0002 0.0004 0.0024 0.0018 0.0009 0.0005 0.0006
Cytoplasm 0.3119 0.4282 0.3684 0.3357 0.3421 0.4842 0.2614 0.3953 0.4698 0.3451 0.277 0.5061 0.2993 0.4431 0.4654 0.3645 0.4254 0.5443
Cytoplasmic Foci 0.0507 0.0252 0.052 0.0369 0.0883 0.048 0.0619 0.0205 0.0381 0.0302 0.0467 0.0515 0.0402 0.0272 0.027 0.0353 0.0357 0.0177
Eisosomes 0.0002 0.0001 0.0005 0 0.0002 0.0001 0.0003 0.0001 0.0001 0.0007 0.0005 0 0.0002 0 0.0003 0.0001 0.0002 0
Endoplasmic Reticulum 0.017 0.0237 0.0163 0.0104 0.0179 0.0129 0.0299 0.0429 0.0215 0.0239 0.0169 0.0181 0.0403 0.0693 0.0444 0.088 0.017 0.0336
Endosome 0.0623 0.0254 0.0781 0.0885 0.1038 0.0391 0.0799 0.0475 0.0753 0.0996 0.149 0.0675 0.0541 0.0489 0.0606 0.0835 0.0704 0.0432
Golgi 0.0045 0.0008 0.0029 0.0008 0.01 0.0015 0.0089 0.0008 0.004 0.0084 0.0185 0.0019 0.0092 0.001 0.0014 0.0012 0.0071 0.0008
Lipid Particles 0.0093 0.004 0.0083 0.0035 0.0483 0.0041 0.0286 0.0086 0.0103 0.0111 0.011 0.0048 0.0263 0.0032 0.0075 0.01 0.0178 0.0015
Mitochondria 0.0035 0.0011 0.0017 0.0023 0.0087 0.002 0.0054 0.0005 0.0045 0.0042 0.0171 0.0013 0.008 0.0006 0.0046 0.0009 0.0074 0.001
None 0.4392 0.4201 0.3719 0.4283 0.1862 0.329 0.3927 0.4011 0.2676 0.2778 0.2585 0.2629 0.4482 0.3127 0.3109 0.2816 0.2244 0.2807
Nuclear Periphery 0.0112 0.0028 0.0078 0.0115 0.0213 0.0121 0.0101 0.0099 0.0075 0.0306 0.023 0.0079 0.0103 0.0087 0.0047 0.0067 0.0051 0.0045
Nucleolus 0.0021 0.0015 0.0016 0.001 0.0064 0.0011 0.0011 0.0006 0.0009 0.0031 0.0007 0.0003 0.0003 0.0004 0.0007 0.0008 0.0016 0.0002
Nucleus 0.0087 0.0059 0.0085 0.0053 0.0137 0.0078 0.0037 0.005 0.0123 0.0173 0.038 0.0115 0.0072 0.0041 0.0046 0.0047 0.0074 0.0058
Peroxisomes 0.0058 0.0005 0.0013 0.0005 0.0261 0.0024 0.0081 0.0001 0.0053 0.0051 0.0087 0.0012 0.0167 0.0004 0.001 0.0007 0.0619 0.0003
Punctate Nuclear 0.0091 0.004 0.0065 0.0187 0.0273 0.0047 0.0074 0.0017 0.003 0.014 0.0056 0.0023 0.0034 0.0021 0.0013 0.0023 0.0106 0.001
Vacuole 0.0322 0.0323 0.0459 0.0471 0.0704 0.0303 0.0482 0.054 0.0472 0.0725 0.0518 0.0529 0.017 0.0594 0.028 0.0605 0.0632 0.0519
Vacuole Periphery 0.0027 0.001 0.0036 0.0079 0.0046 0.0026 0.0035 0.0019 0.0054 0.0062 0.0083 0.0037 0.0014 0.0029 0.0021 0.0036 0.0042 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 263.625 251.3756 262.9014 319.476 226.8768 212.8198 313.7933 323.3555 336.1179 363.3228
Translational Efficiency 0.9999 1.2446 1.1748 1.0747 1.1275 1.1779 1.1551 0.9179 0.9588 0.9712

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to the vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (90%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Vtc4

Vtc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vtc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available