Standard name | |
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Human Ortholog | |
Description | Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 6 | 7 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 4 | 9 | 2 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 17 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 2 | – | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 9 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 1 | 2 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 269 | 159 | 163 | 192 | 277 | 163 | 393 | – | 280 | 316 | 209 | 246 | 240 | 384 | 410 | 101 | 57 | 35 | 288 | 162 | 162 | 171 | 383 | 311 |
Endoplasmic Reticulum | 2 | 3 | 5 | 1 | 5 | 8 | 0 | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 6 | 2 | 4 | 6 | 0 | 3 | 3 | 7 | 8 |
Endosome | 10 | 0 | 1 | 5 | 4 | 0 | 4 | – | 4 | 7 | 5 | 7 | 9 | 20 | 16 | 14 | 17 | 10 | 16 | 3 | 4 | 5 | 17 | 17 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 2 | 1 | 0 | 4 | 4 | 39 | 43 | – | 97 | 139 | 152 | 172 | 2 | 3 | 2 | 3 | 2 | 2 | 4 | 4 | 13 | 9 | 16 | 9 |
Nucleus | 1 | 0 | 1 | 1 | 2 | 6 | 5 | – | 13 | 10 | 13 | 11 | 2 | 0 | 2 | 7 | 2 | 0 | 3 | 2 | 1 | 1 | 3 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 5 | 5 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 6 | – | 5 | 1 | 2 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 7 | 2 | 3 | 3 | 27 | 27 |
Vac/Vac Membrane | 62 | 0 | 2 | 20 | 25 | 4 | 69 | – | 45 | 58 | 38 | 31 | 33 | 71 | 122 | 122 | 85 | 49 | 8 | 5 | 1 | 2 | 8 | 8 |
Unique Cell Count | 321 | 162 | 170 | 216 | 306 | 203 | 476 | 384 | 481 | 361 | 410 | 274 | 453 | 501 | 226 | 139 | 86 | 353 | 189 | 198 | 206 | 510 | 425 | |
Labelled Cell Count | 346 | 164 | 173 | 225 | 319 | 226 | 522 | 452 | 544 | 421 | 477 | 289 | 487 | 556 | 254 | 165 | 100 | 353 | 189 | 198 | 206 | 510 | 425 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 4.9 | 5.8 | 5.4 | 6.0 | 3.9 | 4.9 | – | 4.3 | 4.3 | 4.2 | 4.2 | 5.9 | 5.7 | 6.2 | 7.4 | 8.1 | 7.7 | 5.2 | 5.5 | 5.7 |
Std Deviation (1e-4) | 0.6 | 0.6 | 1.0 | 1.3 | 1.5 | 1.0 | 1.1 | – | 1.0 | 0.9 | 1.0 | 0.7 | 1.2 | 1.5 | 1.4 | 1.2 | 1.3 | 1.2 | 1.6 | 1.4 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.04 | -0.56 | -0.25 | – | -0.44 | -0.42 | -0.46 | -0.47 | 0.02 | -0.02 | 0.08 | 0.35 | 0.48 | 0.41 | -0.17 | -0.09 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 11.2 | 11.7 | 10.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.616 | -0.0594 | -0.2491 | -0.8036 | -0.9523 | -0.3047 | 0.2879 | 0.7297 | 1.0558 | 0.2371 | 0.8135 | 0.8857 | 3.2993 | 3.2003 | 2.8372 | 3.0027 | 2.24 | 2.8988 |
Actin | 0.0164 | 0.0002 | 0.0163 | 0.0015 | 0.0002 | 0.0138 | 0.0265 | 0.0073 | 0.0171 | 0.041 | 0.0558 | 0.003 | 0.0763 | 0.0002 | 0.0169 | 0.0004 | 0.0431 | 0.0101 |
Bud | 0.0003 | 0.0002 | 0.0106 | 0.0287 | 0 | 0.0003 | 0.0003 | 0.0001 | 0.0058 | 0.0007 | 0.0084 | 0.0001 | 0.0015 | 0 | 0.0016 | 0.0001 | 0.0012 | 0 |
Bud Neck | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0016 | 0.0004 | 0.0001 | 0.0005 | 0.0005 | 0.0011 | 0.0006 | 0.009 | 0.0001 | 0.0021 | 0.0001 | 0.0003 | 0.0008 |
Bud Periphery | 0.0006 | 0.0001 | 0.0024 | 0.0063 | 0 | 0.0003 | 0.0004 | 0 | 0.0013 | 0.0021 | 0.0079 | 0.0001 | 0.0019 | 0 | 0.0018 | 0.0001 | 0.0017 | 0.0001 |
Bud Site | 0.0008 | 0.0022 | 0.0055 | 0.0032 | 0.0001 | 0.0005 | 0.0009 | 0.0004 | 0.0026 | 0.0014 | 0.0028 | 0.0001 | 0.0191 | 0.0003 | 0.0067 | 0.0001 | 0.0021 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 |
Cytoplasm | 0.3061 | 0.4744 | 0.3154 | 0.3779 | 0.5699 | 0.4804 | 0.4794 | 0.6606 | 0.6495 | 0.656 | 0.4929 | 0.7855 | 0.3045 | 0.6074 | 0.5416 | 0.4018 | 0.6429 | 0.6454 |
Cytoplasmic Foci | 0.0104 | 0.0139 | 0.0177 | 0.0151 | 0.0053 | 0.0191 | 0.0298 | 0.0053 | 0.0061 | 0.0041 | 0.1242 | 0.007 | 0.0322 | 0.0046 | 0.0055 | 0.0023 | 0.0074 | 0.0072 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0014 | 0.001 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0053 | 0.0072 | 0.0071 | 0.0095 | 0.0037 | 0.0081 | 0.0135 | 0.0048 | 0.006 | 0.0082 | 0.0028 | 0.0124 | 0.0065 | 0.004 | 0.0061 | 0.0011 | 0.0023 | 0.0028 |
Endosome | 0.0061 | 0.0041 | 0.0038 | 0.0104 | 0.0024 | 0.0075 | 0.0154 | 0.002 | 0.0058 | 0.0184 | 0.0735 | 0.0057 | 0.0235 | 0.0016 | 0.006 | 0.0005 | 0.0031 | 0.0016 |
Golgi | 0.0015 | 0.0002 | 0.0012 | 0.0005 | 0.0001 | 0.0023 | 0.0035 | 0.0004 | 0.0054 | 0.0032 | 0.0243 | 0.0037 | 0.0096 | 0.0001 | 0.0011 | 0 | 0.0036 | 0.0006 |
Lipid Particles | 0.0031 | 0.0007 | 0.0057 | 0.0046 | 0.0001 | 0.0016 | 0.014 | 0.0012 | 0.0023 | 0.0008 | 0.0103 | 0.0117 | 0.013 | 0.0002 | 0.0019 | 0.0001 | 0.0041 | 0.0019 |
Mitochondria | 0.0029 | 0.0003 | 0.0017 | 0.0015 | 0.0002 | 0.0017 | 0.0028 | 0.0001 | 0.0042 | 0.0208 | 0.0813 | 0.0015 | 0.0036 | 0.0001 | 0.0006 | 0.0001 | 0.0017 | 0.0003 |
None | 0.6214 | 0.4721 | 0.5748 | 0.4829 | 0.3784 | 0.4245 | 0.3746 | 0.3048 | 0.2767 | 0.1894 | 0.0664 | 0.1526 | 0.4353 | 0.3763 | 0.3929 | 0.5596 | 0.2723 | 0.3059 |
Nuclear Periphery | 0.0115 | 0.0042 | 0.0048 | 0.0136 | 0.0032 | 0.0063 | 0.0098 | 0.0031 | 0.0038 | 0.0172 | 0.0024 | 0.0038 | 0.0102 | 0.0011 | 0.0024 | 0.0016 | 0.0014 | 0.0025 |
Nucleolus | 0.0002 | 0.0003 | 0.0005 | 0.0024 | 0.0001 | 0.0005 | 0.002 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0009 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0046 | 0.012 | 0.0064 | 0.0255 | 0.0335 | 0.0215 | 0.0088 | 0.0044 | 0.0069 | 0.0163 | 0.0035 | 0.0075 | 0.0055 | 0.0025 | 0.0057 | 0.0302 | 0.0027 | 0.0101 |
Peroxisomes | 0.003 | 0.0001 | 0.0085 | 0.0005 | 0 | 0.0005 | 0.0064 | 0.0022 | 0.0021 | 0.0005 | 0.0278 | 0.0006 | 0.0305 | 0 | 0.0011 | 0.0001 | 0.0072 | 0.0069 |
Punctate Nuclear | 0.0023 | 0.0058 | 0.0155 | 0.0118 | 0.0013 | 0.0077 | 0.0086 | 0.0023 | 0.0021 | 0.0013 | 0.0046 | 0.0026 | 0.0126 | 0.0006 | 0.0034 | 0.0013 | 0.0016 | 0.003 |
Vacuole | 0.002 | 0.0015 | 0.0013 | 0.0025 | 0.0009 | 0.0012 | 0.0018 | 0.0008 | 0.001 | 0.0087 | 0.0053 | 0.001 | 0.002 | 0.0007 | 0.0013 | 0.0002 | 0.0008 | 0.0004 |
Vacuole Periphery | 0.0011 | 0.0002 | 0.0003 | 0.0009 | 0.0001 | 0.0004 | 0.0007 | 0.0001 | 0.0003 | 0.0072 | 0.0028 | 0.0005 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.9361 | 5.007 | 7.4308 | 16.1025 | 9.6827 | 20.5258 | 10.547 | 13.0981 | 14.8632 | 15.7823 |
Translational Efficiency | 2.0245 | 4.6612 | 3.015 | 1.5148 | 2.0291 | 1.1027 | 2.3367 | 1.5556 | 1.6001 | 1.5984 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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