Standard name
Human Ortholog
Description UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0.05 0.06 0.07 0 0 0 0 0 0.05 0 0.13 0.21 0.31 0.17 0.09 0.1 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0.06 0.29 0.17 0.2 0.13 0.1 0.14 0.05 0.05 0 0.05 0.05 0 0 0.24 0.23 0.21 0.09 0.09 0.16 0.13 0.19 0.27
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.11 0.13 0.22 0.08 0.18 0.18 0.11 0.11 0.19 0.22 0.21 0 0 0 0 0 0 0.08 0.05 0 0 0 0
Nucleus – 0.09 0.06 0.1 0.11 0.2 0.2 0.22 0.25 0.23 0.26 0.21 0.16 0.26 0.28 0 0.06 0 0 0.06 0 0.07 0.05 0.05
Nuclear Periphery – 0.11 0.07 0.13 0.08 0.17 0.22 0.2 0.28 0.18 0.19 0.18 0.07 0 0 0 0 0 0.63 0.36 0.38 0.21 0.15 0.17
Nucleolus – 0.16 0.23 0.29 0.35 0.28 0.28 0.29 0.33 0.36 0.39 0.35 0 0 0 0.17 0.16 0.17 0 0 0 0 0 0
Peroxisomes – 0 0 0 0.07 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0.05 0.29 0.25 0.38 0.21 0.19 0.25 0.19 0.21 0.12 0.17 0 0 0 0.18 0.13 0.14 0 0.1 0.27 0.31 0.36 0.26
Vac/Vac Membrane – 0.67 0.29 0.16 0.12 0.15 0.07 0.08 0.07 0.07 0.08 0.12 0.65 0.53 0.41 0.49 0.58 0.56 0 0.18 0 0.09 0.06 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 0 2 1 0 2 1 0 0
Bud – 0 8 3 3 4 20 14 6 15 6 9 0 0 0 2 1 1 1 3 6 2 5 6
Bud Neck – 0 0 0 0 0 2 2 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site – 0 2 2 3 5 17 13 10 20 11 15 1 0 0 0 1 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 2 0 0 0 0 0 0
Cytoplasm – 2 12 13 25 14 21 15 15 17 13 9 16 44 77 35 15 14 0 4 4 1 7 7
Endoplasmic Reticulum – 0 0 0 0 1 0 0 0 1 0 2 3 9 11 4 3 6 1 2 6 0 1 2
Endosome – 4 65 40 68 41 56 86 22 31 5 19 6 5 4 48 37 31 3 9 40 5 39 59
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 2 0 0 3
Mitochondria – 7 28 51 26 56 102 66 44 111 63 80 1 1 0 8 7 5 3 4 5 1 7 5
Nucleus – 6 14 24 39 62 119 136 102 136 76 78 20 54 69 7 9 5 1 6 5 3 11 10
Nuclear Periphery – 7 16 31 26 54 130 123 113 110 54 69 8 6 7 2 2 1 27 37 98 9 30 36
Nucleolus – 10 52 66 121 88 161 178 135 216 111 131 2 1 1 34 26 25 0 1 2 0 7 3
Peroxisomes – 0 5 7 25 13 40 40 5 11 2 2 0 0 0 4 0 1 0 0 4 0 1 1
SpindlePole – 3 65 58 130 65 112 158 78 127 35 66 1 2 0 37 20 21 1 10 69 13 73 56
Vac/Vac Membrane – 43 64 36 40 48 38 51 27 39 23 47 79 110 101 99 92 82 0 18 9 3 13 20
Unique Cell Count 64 223 230 346 316 582 621 409 599 287 379 122 209 247 202 159 146 43 104 261 45 206 219
Labelled Cell Count 82 331 331 506 451 818 882 557 835 400 527 138 235 270 282 215 198 43 104 261 45 206 219


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 6.8 6.6 6.3 7.3 6.4 6.7 6.9 7.1 6.8 6.6 6.8 7.6 7.7 7.4 10.3 9.8 10.2 – 8.0 8.4
Std Deviation (1e-4) – 1.5 1.2 1.5 2.0 1.7 1.7 1.8 1.9 1.8 2.0 2.0 1.3 1.1 1.2 3.0 2.5 2.8 – 1.4 1.4
Intensity Change (Log2) – – – -0.05 0.15 -0.04 0.03 0.07 0.1 0.06 -0.0 0.06 0.21 0.24 0.18 0.65 0.58 0.64 – 0.28 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -1.6 0 0 -0.1 -1.1 0 -0.8 -1.0 -0.9 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1.7 0 0 1.9 2.1 2.2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.9 -0.5 -1.1 -2.2 -1.0 -1.8 -0.4 -1.9 2.5 4.8 7.1 3.9 1.5 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Endosome -3.0 -2.6 -4.7 -6.9 -5.1 -8.3 -9.5 -8.9 -8.3 -5.3 -7.5 -8.4 -1.3 -1.3 -1.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.7 -2.0 1.6 1.7 -0.8 -0.7 2.0 2.7 2.6 -3.8 -5.0 -5.7 -3.2 -2.7 -3.0
Nucleus 1.6 2.0 4.4 4.8 5.2 5.8 5.4 5.9 4.7 3.0 5.6 6.1 -1.3 -0.3 -1.2
Nuclear Periphery 2.2 0.2 3.4 5.0 4.4 6.1 4.0 3.8 3.8 -0.2 -2.0 -2.2 -3.2 -2.7 -2.9
Nucleolus 1.3 2.9 1.2 1.3 1.5 2.6 3.5 3.7 2.9 -5.3 -7.2 -7.8 -1.7 -1.7 -1.4
Peroxisomes 0.5 2.6 1.2 2.6 2.4 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.9 2.1 -2.3 -3.0 -1.1 -2.9 -2.4 -4.8 -3.4 -6.4 -8.1 -9.1 -2.6 -3.8 -3.3
Vacuole -3.3 -5.2 -3.8 -8.5 -7.6 -7.6 -8.5 -6.2 -5.0 6.5 5.1 2.8 4.3 5.7 5.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.412 3.3022 3.465 3.6443 2.828 3.0606 1.2463 1.8837 1.2366 1.1698 0.9003 1.5671 0.6235 1.5906 0.8364 1.3093 0.2552 0.5808
Actin 0.0014 0.0006 0.0003 0.0093 0.0847 0.0017 0.0249 0.0027 0.0074 0.0209 0.0686 0.0013 0.0105 0.001 0.0003 0.0294 0.0199 0.0076
Bud 0.0001 0.0001 0 0.0002 0.0002 0.0001 0.0004 0.0001 0.0005 0.0005 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0001
Bud Neck 0.0068 0.0011 0.0002 0.0006 0.0625 0.001 0.0056 0.0006 0.0005 0.0024 0.0004 0.0014 0.0004 0.0002 0.0001 0.0127 0.001 0.0013
Bud Periphery 0.0001 0.0001 0 0.0004 0.0004 0.0002 0.0004 0.0002 0.0007 0.0007 0.0004 0.0001 0.0002 0.0001 0 0.0001 0.0006 0.0001
Bud Site 0.0006 0.0017 0.0002 0.0004 0.0013 0.0001 0.0059 0.0016 0.0022 0.0021 0.0006 0.0001 0.0005 0.0011 0.0001 0.0004 0.0009 0.0002
Cell Periphery 0.0001 0 0 0 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0001
Cytoplasm 0.0107 0.0032 0.0065 0.0052 0.0075 0.0101 0.0074 0.0179 0.0253 0.0084 0.0426 0.0324 0.0052 0.0076 0.002 0.0041 0.0005 0.004
Cytoplasmic Foci 0.0371 0.0094 0.0117 0.0065 0.0244 0.0098 0.0403 0.0271 0.0303 0.0231 0.0386 0.0129 0.0252 0.0349 0.0227 0.0265 0.033 0.0153
Eisosomes 0 0 0 0.0001 0.0006 0 0.0003 0 0.0001 0.0002 0.0002 0 0.0003 0 0 0.0002 0.0002 0
Endoplasmic Reticulum 0.0061 0.0077 0.0067 0.003 0.0043 0.0052 0.0175 0.0103 0.0102 0.0095 0.0107 0.0074 0.0069 0.0068 0.0043 0.0021 0.0014 0.0099
Endosome 0.018 0.0077 0.0061 0.0138 0.0088 0.0062 0.0326 0.0082 0.0179 0.0401 0.0513 0.0134 0.0242 0.006 0.0042 0.0067 0.0164 0.0099
Golgi 0.0023 0.0012 0.0011 0.0022 0.0045 0.0018 0.0127 0.0037 0.0069 0.0116 0.0152 0.0021 0.0048 0.0017 0.0014 0.0037 0.0076 0.0027
Lipid Particles 0.0322 0.0259 0.0235 0.0058 0.0208 0.0277 0.0844 0.0614 0.0402 0.0383 0.0917 0.0415 0.0392 0.0463 0.0282 0.0131 0.0206 0.0452
Mitochondria 0.0005 0.0004 0.0002 0.0051 0.0009 0.0024 0.005 0.0027 0.0075 0.0077 0.0097 0.0007 0.0022 0.0004 0.0001 0.0004 0.0032 0.0003
None 0.0263 0.0008 0.0061 0.0025 0.0076 0.008 0.0026 0.0009 0.0019 0.003 0.0036 0.0069 0.0028 0.001 0.0003 0.0009 0.0009 0.0007
Nuclear Periphery 0.3649 0.3481 0.2 0.1925 0.1919 0.3992 0.2256 0.1857 0.2018 0.15 0.1782 0.3477 0.3597 0.2205 0.2001 0.0434 0.0144 0.337
Nucleolus 0.008 0.0345 0.0244 0.0038 0.0353 0.0223 0.0112 0.0316 0.0079 0.0445 0.0013 0.0114 0.0046 0.0351 0.0098 0.0258 0.0611 0.016
Nucleus 0.0185 0.0613 0.0293 0.0479 0.0329 0.0563 0.0281 0.0467 0.0463 0.0471 0.0345 0.0666 0.0392 0.0414 0.0244 0.0055 0.005 0.0462
Peroxisomes 0.0015 0.0006 0.0004 0.0014 0.0025 0.001 0.0124 0.0014 0.0035 0.0041 0.0031 0.0014 0.0016 0.0009 0.0006 0.0026 0.0014 0.0013
Punctate Nuclear 0.4583 0.4902 0.6804 0.6946 0.5051 0.4426 0.4768 0.594 0.5816 0.5776 0.4416 0.4469 0.4661 0.5907 0.6987 0.8217 0.81 0.4985
Vacuole 0.0017 0.0016 0.0009 0.0018 0.0012 0.0013 0.0023 0.001 0.0039 0.0031 0.0039 0.0019 0.0017 0.0011 0.0004 0.0003 0.0007 0.0012
Vacuole Periphery 0.0046 0.004 0.002 0.0028 0.0028 0.003 0.0035 0.0021 0.0034 0.0048 0.0035 0.0036 0.0044 0.0031 0.0022 0.0004 0.0008 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1174 8.2819 5.531 9.6967 8.4083 5.5556 8.1645 14.9772 10.1781 10.2943
Translational Efficiency 0.6354 0.7256 1.0574 0.8682 0.9283 1.8956 0.8244 0.5656 1.01 0.7701

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication
Localization
Cell Percentages nuclear periphery (43%), spindle pole (12%), mixed (7%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ubx6

Ubx6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubx6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available