Standard name
Human Ortholog
Description Integral plasma membrane protein; involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0.06 0.15 0 0 0 0 0 0
Cytoplasm 0.12 0.13 0.19 0.19 0.17 0.22 0.19 0.22 0.19 0.21 0.18 0.24 0.23 0.32 0.41 0.46 0.41 0 0 0 0.06 0.06 0.07
Endoplasmic Reticulum 0.4 0.74 0.41 0.32 0.19 0.17 0.12 0.13 0.1 0.09 0.1 0.75 0.77 0.58 0 0 0 0.85 0.82 0.78 0.65 0.64 0.66
Endosome 0 0 0.07 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.15 0.41 0.32 0.56 0.61 0.6 0.72 0.67 0.71 0 0 0 0.14 0.16 0.1 0 0 0.05 0.16 0.11 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Nuclear Periphery 0 0.06 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0.05 0.09 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.58 0.17 0.3 0.21 0.4 0.24 0.25 0.21 0.19 0.15 0.18 0.1 0.1 0.08 0.34 0.24 0.25 0 0 0 0.06 0.1 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 9 4 2 1 0 0 2 1 1 0 0 1 0 11 5 5 3 4 9 0 4 1
Bud 1 0 1 1 1 9 8 12 9 10 9 0 0 0 1 0 1 0 1 1 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0
Bud Site 0 0 0 0 0 2 0 1 2 0 2 0 0 0 0 0 0
Cell Periphery 4 6 5 8 5 6 9 12 7 19 10 2 2 2 17 9 18 0 2 1 0 3 1
Cytoplasm 38 41 51 35 36 85 87 116 95 72 74 14 14 17 111 70 50 8 12 10 8 11 14
Endoplasmic Reticulum 126 231 108 59 41 67 56 68 50 31 43 44 46 31 6 2 5 326 305 281 89 122 132
Endosome 4 2 18 4 19 4 9 5 1 1 1 2 0 1 11 3 1 2 3 4 3 1 1
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1 1 3 0 5 1 2 2
Mitochondria 10 13 38 76 69 223 287 323 365 224 293 0 0 0 38 24 12 8 10 19 21 21 14
Nucleus 3 1 0 0 1 7 6 12 4 15 13 1 1 2 5 11 4 0 0 0 0 0 1
Nuclear Periphery 13 20 11 2 6 12 20 21 27 15 24 0 1 1 5 8 11 16 14 10 0 3 0
Nucleolus 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 1 0 0 0 0 1 0 0 0 0 0 3 1 1 0 0 0 0 0 0
Vac/Vac Membrane 180 54 79 38 86 96 116 114 98 51 76 6 6 4 91 36 30 11 13 9 7 18 24
Unique Cell Count 313 314 262 185 217 395 467 539 509 335 414 59 60 53 271 153 121 384 373 359 138 193 200
Labelled Cell Count 383 377 317 228 265 511 598 687 660 440 545 69 71 58 302 170 139 384 373 359 138 193 200


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 7.4 6.6 6.1 6.2 5.1 5.0 5.2 4.5 4.7 4.7 8.3 8.3 8.3 6.5 7.0 8.2 7.6 8.5 9.0
Std Deviation (1e-4) 0.9 1.0 1.2 1.4 1.5 1.0 1.3 1.3 1.3 1.1 0.9 1.1 1.1 1.3 1.0 1.1 1.3 1.1 2.1 1.9
Intensity Change (Log2) -0.1 -0.09 -0.38 -0.41 -0.35 -0.54 -0.5 -0.49 0.32 0.32 0.33 -0.01 0.08 0.31 0.2 0.37 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1.8 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.5 0 0 0 0.3 0 0 0 0 0 0 2.5 2.2 5.0
Cytoplasm -0.1 -0.8 0.6 -0.3 0.7 -0.3 0.6 -0.5 0.7 0.7 2.0 5.4 5.7 4.5
Endoplasmic Reticulum -2.0 -5.3 -6.9 -9.1 -9.2 0 0 0 4.6 5.0 2.3 -11.0 -8.9 -7.4
Endosome -2.3 0.8 -4.1 -3.4 -4.7 0 0 0 0 0 0 -1.4 -2.2 -2.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.3 4.5 10.8 12.2 12.1 0 0 0 -3.1 -3.1 -3.0 -0.2 0.3 -1.2
Nucleus 0 0 0 0 0 0 3.5 2.9 0 0 0 0 0 0
Nuclear Periphery -1.9 -0.8 -0.8 0.1 -0.2 0 0 0 0 0 0 -1.6 0.5 1.9
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.3 2.2 -1.7 -1.6 -2.8 -3.4 -4.4 -3.6 -3.1 -3.2 -3.4 0.8 -1.5 -1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.89 4.3221 4.0197 4.1701 3.6173 4.0926 6.0938 5.8283 5.9956 5.6317 5.455 6.1611 5.6155 5.7802 5.198 5.4883 4.4644 5.4739
Actin 0.0004 0.0005 0.001 0.0003 0.0262 0.0008 0.0137 0.0004 0.0092 0.0173 0.002 0.0006 0.1532 0.0021 0.0084 0.0001 0.0021 0.0008
Bud 0 0.0001 0 0 0.0005 0.0001 0.001 0.0001 0.0001 0.0002 0.0003 0 0.002 0.0001 0 0 0.0001 0
Bud Neck 0.0001 0.0002 0.0002 0.0006 0.0024 0.001 0.0045 0.0003 0.0023 0.0002 0.0005 0.0007 0.0047 0.0006 0.0002 0.0001 0.0004 0.0003
Bud Periphery 0 0.0001 0.0001 0 0.0007 0.0003 0.0013 0.0001 0.0003 0.0003 0.0005 0.0001 0.0046 0.0001 0.0001 0 0.0001 0.0001
Bud Site 0.0001 0.0005 0.0003 0 0.0014 0.0001 0.0174 0.0011 0.0016 0.0002 0.0005 0.0001 0.0367 0.0132 0.0003 0 0.0001 0
Cell Periphery 0.0001 0.0003 0.0002 0.0001 0.0004 0.0001 0.0005 0.0005 0.0009 0.0003 0.0002 0.0003 0.0042 0.0032 0.0009 0.0002 0.0002 0.0003
Cytoplasm 0.0642 0.1834 0.1008 0.0481 0.1027 0.0892 0.0569 0.1201 0.0733 0.0776 0.1302 0.0993 0.0954 0.1169 0.0937 0.0109 0.0623 0.1437
Cytoplasmic Foci 0.0014 0.0028 0.0031 0.0005 0.0037 0.0027 0.0214 0.0071 0.0034 0.0051 0.0048 0.0025 0.0048 0.0017 0.0049 0.0002 0.0028 0.0047
Eisosomes 0 0 0 0 0.0001 0 0.0003 0 0.0003 0 0 0 0.0054 0.0001 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.6677 0.6117 0.717 0.834 0.6752 0.6606 0.6569 0.7015 0.7753 0.7863 0.6072 0.7575 0.6289 0.7862 0.8233 0.9048 0.7453 0.7253
Endosome 0.0164 0.0261 0.0205 0.0068 0.0188 0.0213 0.0515 0.0516 0.0135 0.0461 0.053 0.0209 0.0105 0.0111 0.0139 0.0128 0.0605 0.0124
Golgi 0.0003 0.0007 0.0006 0.0001 0.0019 0.0004 0.0129 0.0024 0.0028 0.0053 0.0116 0.0011 0.0055 0.0011 0.0024 0.0002 0.0112 0.0012
Lipid Particles 0.0018 0.0014 0.0026 0.0007 0.0024 0.0008 0.014 0.0052 0.0093 0.0026 0.0031 0.0016 0.0093 0.0024 0.0063 0.0003 0.0028 0.009
Mitochondria 0.0006 0.0008 0.0005 0.0003 0.0016 0.0013 0.005 0.0007 0.0016 0.0038 0.0338 0.0019 0.0095 0.0006 0.0003 0.0006 0.0105 0.0031
None 0.0054 0.0025 0.0022 0.0005 0.0136 0.0081 0.0145 0.0105 0.0459 0.004 0.0022 0.0282 0.0028 0.0034 0.0062 0.0002 0.0018 0.0508
Nuclear Periphery 0.2058 0.12 0.1193 0.088 0.1124 0.1629 0.0842 0.0686 0.0474 0.0262 0.1114 0.0646 0.0129 0.0483 0.0303 0.0583 0.0762 0.039
Nucleolus 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0016 0.0002 0.0002 0.0001 0.0001 0.0001 0.0005 0 0 0 0 0.0001
Nucleus 0.0238 0.0301 0.0191 0.0121 0.0204 0.0379 0.0095 0.0115 0.0042 0.0018 0.0143 0.0097 0.0011 0.0036 0.0023 0.0024 0.0158 0.0024
Peroxisomes 0 0 0.0001 0 0.0048 0 0.008 0.0001 0.0012 0.0151 0.0009 0.0001 0.0027 0 0.0001 0 0.0002 0.0002
Punctate Nuclear 0.0011 0.0012 0.0023 0.0004 0.0018 0.0012 0.0152 0.0017 0.0013 0.0006 0.0008 0.0007 0.0005 0.0003 0.0004 0.0001 0.0003 0.0004
Vacuole 0.0058 0.0126 0.0076 0.0055 0.0066 0.0078 0.0067 0.0141 0.0045 0.0047 0.0177 0.0077 0.004 0.0039 0.0051 0.0059 0.0045 0.004
Vacuole Periphery 0.0046 0.0049 0.0023 0.0019 0.0021 0.0034 0.0031 0.0023 0.0015 0.0023 0.0048 0.0021 0.0008 0.0011 0.0009 0.0029 0.0026 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.8839 20.9699 22.3133 26.3038 24.6756 22.1178 26.3033 23.3556 26.5674 32.1432
Translational Efficiency 0.9177 0.8872 0.8267 0.7294 0.798 1.0584 0.8328 0.7209 0.6903 0.6162

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
193 1217 160 1540 2124 1449 207 1517 2317 2666 367 3057

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 931.31 843.71 1137.28 1018.01 820.00 872.47 1056.43 876.54 829.27 859.34 1091.68 947.81
Standard Deviation 137.36 124.46 134.67 135.94 98.50 111.94 125.30 120.66 106.82 118.69 135.53 146.76
Intensity Change Log 2 -0.142514 0.288254 0.128418 0.089482 0.365501 0.096196 -0.029234 0.324938 0.113421

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001471 0.000260 0.000311 0.001817 0.000087 0.000079 0.000265 0.000453 0.000202 0.000162 0.000285 0.001140
Bud Neck 0.003556 0.001055 0.000955 0.006041 0.000476 0.000361 0.000812 0.009162 0.000732 0.000678 0.000874 0.007590
Bud Site 0.007467 0.002343 0.001043 0.010537 0.001490 0.000435 0.001883 0.015825 0.001988 0.001306 0.001517 0.013161
Cell Periphery 0.000249 0.000172 0.000237 0.000541 0.000241 0.000130 0.000204 0.000424 0.000242 0.000149 0.000218 0.000483
Cytoplasm 0.086780 0.100600* 0.060002 0.102349* 0.097525 0.072377 0.036427 0.309718* 0.096630 0.085261 0.046705 0.205254*
Cytoplasmic Foci 0.038440 0.026887 0.010169 0.012613 0.018036 0.014077 0.002607 0.060927 0.019735 0.019924 0.005904 0.036589
Eisosomes 0.000092 0.000039 0.000026 0.000095 0.000060 0.000040 0.000021 0.000144 0.000063 0.000039 0.000023 0.000119
Endoplasmic Reticulum 0.222944* 0.577433* 0.452739* 0.554124* 0.666558* 0.645740* 0.707603* 0.038998 0.629606* 0.614558* 0.596491* 0.298499*
Endosome 0.035708 0.009565 0.014689 0.015630 0.005767 0.007242 0.007219 0.016603 0.008261 0.008303 0.010476 0.016113
Golgi 0.022768 0.004818 0.000357 0.003852 0.004785 0.004423 0.000390 0.002220 0.006283 0.004603 0.000375 0.003042
Lipid Particles 0.051946 0.036597 0.009785 0.017877 0.059867 0.051544 0.004965 0.024298 0.059207 0.044721 0.007067 0.021063
Mitochondria 0.003885 0.006215 0.000434 0.003234 0.006183 0.010391 0.000498 0.009200 0.005992 0.008484 0.000470 0.006194
Mitotic Spindle 0.019494 0.002315 0.000507 0.011088 0.000197 0.000744 0.000296 0.012929 0.001804 0.001461 0.000388 0.012001
None 0.001306 0.005325 0.000284 0.000925 0.004943 0.004190 0.000446 0.010175 0.004640 0.004708 0.000375 0.005515
Nuclear Periphery 0.123483* 0.082888 0.137510* 0.045659 0.065335 0.099899 0.082284 0.087907 0.070179 0.092134 0.106361* 0.066624
Nuclear Periphery Foci 0.167601 0.101779 0.239631* 0.114765 0.038725 0.060509 0.116344 0.051878 0.049460 0.079348 0.170093 0.083558
Nucleolus 0.002900 0.001066 0.000302 0.000449 0.000339 0.000756 0.000071 0.006141 0.000553 0.000898 0.000172 0.003274
Nucleus 0.034677 0.020475 0.021587 0.030537 0.017328 0.013736 0.016319 0.245118* 0.018774 0.016812 0.018615 0.137020*
Peroxisomes 0.001964 0.001009 0.000184 0.000741 0.001284 0.000986 0.000131 0.003939 0.001341 0.000996 0.000154 0.002328
Vacuole 0.160929 0.015879 0.048543 0.065391 0.009169 0.009392 0.020900 0.091703 0.021810 0.012353 0.032952 0.078448
Vacuole Periphery 0.012340 0.003281 0.000704 0.001735 0.001607 0.002949 0.000315 0.002238 0.002501 0.003101 0.000485 0.001985

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.88 1.78 -0.57 -2.85 -2.74 0.30 -1.47 -5.93 -6.19 -1.41 0.59 -1.02 -3.36 -3.53 -2.98
Bud Neck 4.27 4.60 -3.60 -8.15 -8.56 0.77 -1.73 -10.97 -11.15 -10.31 0.45 -0.81 -13.86 -13.84 -13.41
Bud Site 2.74 3.28 -0.82 -6.21 -7.54 2.03 -0.69 -6.70 -7.44 -5.87 1.45 0.56 -8.65 -9.82 -8.86
Cell Periphery 2.85 0.32 -2.83 -3.75 -2.82 1.57 0.28 -2.07 -7.38 -3.24 1.45 0.19 -2.87 -6.00 -3.99
Cytoplasm -0.47 1.93 -1.04 -1.10 -3.78 5.08 8.40 -26.13 -29.02 -27.96 2.64 7.08 -19.20 -21.88 -20.56
Cytoplasmic Foci 2.08 5.16 4.98 5.37 -1.27 2.17 11.15 -15.77 -16.82 -22.80 -0.19 8.24 -9.75 -9.35 -17.21
Eisosomes 4.56 5.20 -0.14 -3.24 -3.74 2.81 4.62 -10.30 -12.65 -13.22 4.06 5.95 -5.41 -7.87 -8.96
Endoplasmic Reticulum -17.49 -8.30 -15.15 4.12 -2.91 1.93 -1.82 89.05* 70.28* 34.10* 1.45 1.97 36.71 36.71 17.81
Endosome 6.46 4.79 4.81 -5.78 -0.57 -1.80 -1.61 -9.42 -7.54 -5.87 -0.14 -2.32 -9.08 -9.59 -4.63
Golgi 2.05 2.68 2.21 1.04 -3.31 0.41 7.90 3.87 2.82 -3.10 1.55 7.18 3.39 2.46 -4.35
Lipid Particles 1.60 5.13 3.94 6.47 -4.78 2.19 20.51 12.52 8.47 -10.04 4.67 20.67 14.77 10.76 -10.73
Mitochondria -2.08 2.85 0.26 2.87 -3.71 -2.36 6.96 -1.29 1.09 -5.83 -2.12 7.34 0.32 2.40 -6.88
Mitotic Spindle 3.03 3.29 1.78 -4.67 -5.87 -2.30 -0.61 -7.09 -6.78 -7.02 1.14 2.89 -7.01 -8.17 -9.14
None -3.62 1.40 0.44 4.57 -2.88 0.69 5.03 -2.95 -3.66 -6.93 -0.09 5.35 -0.49 -0.48 -7.21
Nuclear Periphery 2.12 -1.27 5.22 8.16 6.08 -6.73 -1.52 -2.49 3.25 0.24 -5.58 -3.89 2.07 7.66 4.76
Nuclear Periphery Foci 3.57 -3.79 2.88 -1.74 7.74 -6.02 -7.18 -2.87 2.51 6.03 -8.57 -12.26 -9.22 -0.55 8.82
Nucleolus 0.87 1.33 1.22 2.09 -1.23 -1.43 4.46 -8.16 -7.11 -8.56 -1.52 2.22 -7.06 -5.87 -8.60
Nucleus 2.69 1.83 0.27 -5.59 -2.23 2.17 0.12 -36.16 -36.48 -29.63 1.33 -0.09 -30.85 -31.44 -23.50
Peroxisomes 1.24 2.04 1.46 0.97 -2.61 1.23 5.75 -4.39 -5.01 -6.67 1.67 6.05 -2.63 -4.15 -7.14
Vacuole 10.17 7.70 6.13 -15.86 -4.40 -0.18 -2.75 -22.74 -22.49 -13.21 5.83 -2.34 -20.42 -27.26 -12.39
Vacuole Periphery 1.92 2.59 2.33 2.53 -2.02 -2.74 7.78 -1.20 1.71 -5.63 -1.50 5.19 1.19 2.98 -4.80
Standard name
Human Ortholog
Description Integral plasma membrane protein; involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity
Localization
Cell Percentages ER (85%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Las21

Las21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Las21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available