Standard name
Human Ortholog
Description Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.08 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.97 0.95 0.96 0.87 0.8 0.79 0.74 0.71 0.64 0.64 0.98 1.0 0.99 0.26 0.18 0.19 0.72 0.73 0.66 0.6 0.66 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.15 0.13 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Mitochondria 0.05 0 0.05 0.07 0.09 0.23 0.37 0.34 0.47 0.41 0.54 0.49 0 0 0 0.2 0.16 0.17 0.11 0.14 0.18 0.14 0.19 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.17 0.14 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.19 0.2 0.15 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.15 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 11 6 6 1 1 7 2 2 4
Bud 2 0 0 1 0 9 7 9 11 13 13 7 0 0 0 11 5 11 2 1 0 0 1 6
Bud Neck 1 0 0 0 3 10 11 8 3 5 4 5 1 1 1 2 3 1 1 0 0 2 1 4
Bud Site 0 0 0 0 0 3 1 2 1 6 6 4 0 0 0 1 4 0 – – – – – –
Cell Periphery 1 0 1 1 1 3 0 2 2 1 2 1 0 3 0 5 14 6 2 2 3 0 2 3
Cytoplasm 203 203 271 266 306 413 411 430 327 268 295 292 158 209 241 46 27 25 179 170 176 60 118 172
Endoplasmic Reticulum 2 1 2 0 0 3 1 1 0 0 0 0 3 1 2 15 7 6 1 1 5 1 2 3
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 27 23 17 8 4 2 2 1 2
Golgi 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 6 4 10 2 2 0 0 1 1
Mitochondria 11 5 15 19 30 109 191 188 210 155 248 226 0 0 0 35 24 22 28 33 47 14 34 30
Nucleus 0 0 0 1 0 2 3 4 1 6 0 1 0 0 1 3 7 3 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 2 2 0 1 2 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Nucleolus 1 0 0 1 0 0 1 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 16 26 18 0 0 0 0 0 0
SpindlePole 0 0 0 6 5 5 16 22 10 24 17 20 1 0 0 34 30 20 4 3 4 1 3 4
Vac/Vac Membrane 1 1 1 1 0 0 1 0 0 0 2 1 0 1 0 12 12 20 8 4 4 6 4 10
Unique Cell Count 216 209 279 280 318 475 515 547 443 377 458 458 162 210 244 178 149 133 249 233 268 100 180 251
Labelled Cell Count 224 212 290 296 345 559 645 667 566 482 589 558 163 215 246 224 193 166 249 233 268 100 180 251


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.7 3.0 3.0 2.9 2.8 2.4 2.6 2.4 2.4 2.3 2.2 4.8 4.3 4.6 5.2 5.5 5.5 4.9 4.9 5.3
Std Deviation (1e-4) 0.5 0.8 0.7 1.4 1.3 1.1 1.4 1.3 1.3 1.1 1.0 0.7 1.3 0.9 1.1 0.9 1.2 1.3 1.8 1.2 2.1
Intensity Change (Log2) – – – 0.01 -0.04 -0.09 -0.29 -0.18 -0.3 -0.33 -0.41 -0.44 0.67 0.51 0.62 0.79 0.86 0.88 0.69 0.7 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 4.0 – 6.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5881 -0.243 -0.4962 -0.5937 -0.6684 -0.1661 0.8781 1.1892 1.6094 1.0821 1.3022 1.3619 -2.5917 -1.7064 -1.9546 -1.7663 -1.1482 -1.9711
Actin 0.0014 0.0039 0.001 0.0005 0.0018 0.0025 0.0327 0.0003 0.0235 0.0019 0.0014 0.0027 0.0015 0.0003 0.001 0.0004 0 0
Bud 0.0008 0.0037 0.0043 0.0014 0.0048 0.0008 0.0012 0.0004 0.0022 0.0002 0.0004 0.0004 0 0 0.0001 0 0 0
Bud Neck 0.0009 0.0041 0.0021 0.0002 0.0045 0.0041 0.0005 0.0001 0.0025 0.0049 0.0013 0.0006 0.0001 0 0.0001 0 0 0
Bud Periphery 0.0015 0.0058 0.0022 0.0008 0.0082 0.0015 0.0035 0.002 0.0053 0.0007 0.001 0.0008 0 0.0001 0.0005 0.0001 0 0
Bud Site 0.0058 0.0233 0.0106 0.0005 0.005 0.0017 0.0014 0.0005 0.0092 0.0018 0.0005 0.0007 0 0.0001 0.0003 0 0 0
Cell Periphery 0.0004 0.0015 0.0004 0.0001 0.0002 0.0002 0.0006 0.0008 0.0017 0.0006 0.0003 0.0002 0.0001 0.0002 0.0006 0.0001 0 0
Cytoplasm 0.0763 0.0828 0.0686 0.0414 0.0801 0.0771 0.0433 0.0781 0.0674 0.0761 0.1733 0.0814 0.0343 0.0492 0.0667 0.0419 0.0117 0.0489
Cytoplasmic Foci 0.0642 0.0637 0.0503 0.0622 0.0307 0.06 0.0634 0.039 0.0403 0.061 0.0457 0.0381 0.0101 0.0212 0.0186 0.0028 0.0444 0.0044
Eisosomes 0.0004 0.0004 0.0003 0.0002 0.0001 0.0002 0.0005 0.0004 0.0008 0.0004 0.0002 0.0002 0.0004 0.0004 0.0009 0.0005 0 0
Endoplasmic Reticulum 0.0018 0.0029 0.0017 0.0006 0.0009 0.0018 0.0013 0.0018 0.0015 0.0011 0.0011 0.0014 0.0009 0.0014 0.001 0.0008 0.0003 0.0002
Endosome 0.0278 0.0333 0.0214 0.0176 0.0225 0.0173 0.0254 0.0069 0.0156 0.0145 0.0308 0.0081 0.0012 0.0026 0.0123 0.0007 0.273 0.0003
Golgi 0.0049 0.0057 0.0026 0.0009 0.003 0.0025 0.0044 0.0015 0.0062 0.0059 0.0024 0.0011 0.0007 0.0003 0.002 0.0002 0.0063 0
Lipid Particles 0.0104 0.0054 0.0033 0.0019 0.0009 0.0032 0.012 0.0013 0.0037 0.015 0.0012 0.0043 0.0011 0.0008 0.018 0.0008 0.0006 0.0002
Mitochondria 0.016 0.0267 0.0264 0.0027 0.0127 0.0237 0.0098 0.0241 0.0066 0.0086 0.0037 0.0123 0.0003 0.0007 0.0151 0.0004 0.0003 0.0001
None 0.7617 0.712 0.7892 0.8586 0.7889 0.7822 0.7684 0.8371 0.7953 0.785 0.6836 0.8279 0.9482 0.9185 0.8425 0.9501 0.6603 0.9449
Nuclear Periphery 0.0014 0.0018 0.0008 0.0007 0.0005 0.0008 0.004 0.0003 0.0012 0.0004 0.0104 0.0058 0.0001 0.0002 0.0003 0.0003 0.0002 0.0001
Nucleolus 0.0017 0.0013 0.001 0.001 0.0003 0.0005 0.0014 0.0004 0.0005 0.001 0.0013 0.0002 0.0001 0.0001 0.0001 0 0 0
Nucleus 0.0023 0.0035 0.002 0.0012 0.0016 0.0022 0.0019 0.0004 0.001 0.0009 0.0225 0.0027 0.0001 0.0002 0.0002 0.0001 0 0.0001
Peroxisomes 0.0111 0.0101 0.0043 0.0021 0.0293 0.0097 0.0099 0.0012 0.0091 0.0154 0.0019 0.0082 0.0004 0.0017 0.0178 0.0002 0.0004 0
Punctate Nuclear 0.0042 0.0031 0.0039 0.0031 0.0014 0.005 0.0057 0.0002 0.0015 0.0014 0.0114 0.0007 0.0002 0.0002 0.0003 0.0001 0.0001 0.0004
Vacuole 0.0041 0.004 0.0029 0.0015 0.0021 0.0023 0.0071 0.0025 0.0042 0.0026 0.0047 0.0015 0.0003 0.0015 0.0012 0.0003 0.0013 0.0002
Vacuole Periphery 0.0009 0.0009 0.0007 0.0006 0.0006 0.0008 0.0018 0.0006 0.0007 0.0005 0.001 0.0006 0 0.0002 0.0004 0.0001 0.001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 0.5151 3.6839 5.4673 8.0064 5.3431 1.0297 6.2857 6.5993 8.3659 9.6401
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
35 1594 1862 146 1803 1662 1545 1960 1838 3256 3407 2106

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 707.12 696.96 735.63 876.54 681.99 678.75 792.02 852.11 682.47 687.66 761.20 853.80
Standard Deviation 69.33 113.08 85.39 92.03 73.81 83.84 85.05 107.76 73.81 99.66 89.74 106.92
Intensity Change Log 2 — -0.020879 0.057025 0.309865 — -0.006870 0.215786 0.321289 — -0.013984 0.137153 0.315485

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000045 0.000289 0.000515 0.001179 0.000062 0.000478 0.000131 0.000507 0.000061 0.000386 0.000341 0.000554
Bud Neck 0.003125 0.043862 0.012531 0.012383 0.018487 0.052028 0.005334 0.014440 0.018195 0.048030 0.009267 0.014297
Bud Site 0.000604 0.016247 0.012537 0.031284 0.002944 0.014291 0.004956 0.030056 0.002900 0.015249 0.009099 0.030141
Cell Periphery 0.000256 0.000288 0.000093 0.000163 0.000130 0.000191 0.000101 0.000138 0.000132 0.000238 0.000097 0.000140
Cytoplasm 0.422621* 0.387111* 0.446393* 0.314270* 0.442199* 0.328716* 0.393347* 0.478074* 0.441826* 0.357304* 0.422338* 0.466718*
Cytoplasmic Foci 0.349274* 0.238778* 0.033723 0.061372 0.276091* 0.317809* 0.030005 0.039233 0.277484* 0.279119* 0.032037 0.040768
Eisosomes 0.000257 0.000127 0.000033 0.000108 0.000187 0.000252 0.000044 0.000051 0.000189 0.000191 0.000038 0.000055
Endoplasmic Reticulum 0.001250 0.000683 0.001654 0.003738 0.000865 0.000618 0.001073 0.001096 0.000872 0.000649 0.001391 0.001279
Endosome 0.016659 0.022233 0.005242 0.019777 0.018260 0.029997 0.003615 0.004156 0.018229 0.026196 0.004504 0.005239
Golgi 0.001678 0.004188 0.001054 0.000492 0.002415 0.007277 0.000248 0.002256 0.002401 0.005764 0.000689 0.002134
Lipid Particles 0.019333 0.008960 0.001679 0.003040 0.016785 0.015043 0.002568 0.001389 0.016833 0.012065 0.002082 0.001503
Mitochondria 0.000976 0.017256 0.003435 0.000595 0.006310 0.024100 0.001400 0.002319 0.006208 0.020750 0.002512 0.002200
Mitotic Spindle 0.000025 0.004487 0.010015 0.019151 0.000571 0.005899 0.006882 0.025830 0.000561 0.005208 0.008594 0.025367
None 0.002402 0.005182 0.005024 0.004884 0.002566 0.001805 0.005044 0.003885 0.002563 0.003458 0.005033 0.003954
Nuclear Periphery 0.000537 0.000714 0.001411 0.002204 0.000521 0.000363 0.001476 0.001279 0.000521 0.000535 0.001441 0.001343
Nuclear Periphery Foci 0.000454 0.000375 0.000873 0.003427 0.000273 0.000226 0.000579 0.000345 0.000276 0.000299 0.000740 0.000559
Nucleolus 0.001804 0.002777 0.001747 0.001618 0.001452 0.002450 0.001851 0.000478 0.001458 0.002610 0.001794 0.000557
Nucleus 0.140159* 0.155121* 0.420293* 0.238478* 0.154940* 0.109191 0.501840* 0.296129* 0.154659* 0.131676* 0.457273* 0.292132*
Peroxisomes 0.003662 0.019575 0.003417 0.014331 0.006562 0.028903 0.002095 0.008302 0.006507 0.024336 0.002817 0.008720
Vacuole 0.034026 0.069156 0.037103 0.267132* 0.047222 0.057863 0.037047 0.088348 0.046971 0.063391 0.037078 0.100743
Vacuole Periphery 0.000851 0.002593 0.001227 0.000374 0.001159 0.002500 0.000362 0.001687 0.001153 0.002545 0.000835 0.001596

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.26 -3.23 -3.70 -2.31 -0.73 -3.19 -3.84 -6.95 0.53 -4.89 -4.76 -3.54 -7.65 -0.46 -0.68
Bud Neck -15.89 -6.75 -2.50 12.75 3.04 -10.90 9.33 4.01 13.21 -5.21 -13.37 6.09 4.20 17.01 -1.71
Bud Site -10.42 -7.96 -2.46 0.49 -0.26 -7.82 -1.80 -8.70 -2.42 -7.17 -11.17 -5.93 -8.92 -2.08 -5.32
Cell Periphery -0.35 3.38 2.29 1.94 -3.06 -4.09 4.76 2.88 5.38 -1.30 -2.85 6.32 2.99 3.41 -2.36
Cytoplasm 0.83 -0.20 4.31 7.82 9.98 12.90 8.65 3.15 -9.72 -5.66 11.11 5.65 4.73 -5.90 -0.46
Cytoplasmic Foci 3.02 8.52 8.46 26.85 0.08 -5.48 46.09 45.78 47.90 -1.57 -0.17 47.61 46.59 56.49 -0.57
Eisosomes 3.50 5.94 3.69 0.95 -9.86 -4.17 17.20 16.35 14.67 -2.78 -0.12 18.87 16.22 17.22 -8.31
Endoplasmic Reticulum 0.71 -0.98 -4.37 -7.13 -5.24 2.08 -4.52 -5.33 -8.91 -0.85 1.96 -6.83 -7.12 -11.42 -0.84
Endosome -1.10 2.34 1.17 3.33 -1.28 -5.52 10.50 11.05 16.04 0.77 -4.60 10.14 10.74 19.29 1.64
Golgi -3.41 1.13 2.37 9.78 2.82 -6.82 9.96 2.58 7.57 -2.25 -7.44 7.27 2.86 8.19 -1.22
Lipid Particles 2.32 4.00 3.74 8.60 -3.10 1.17 12.15 13.85 13.34 2.46 3.84 13.36 14.03 17.20 2.46
Mitochondria -9.55 -3.08 1.62 10.05 4.24 -8.91 6.89 6.33 11.97 -1.32 -10.07 4.96 6.45 15.08 1.84
Mitotic Spindle -4.82 -6.60 -1.86 -1.01 0.03 -3.80 -4.03 -8.33 -4.93 -4.79 -5.31 -7.24 -8.51 -5.76 -4.10
None -2.38 -2.39 -1.13 0.79 0.54 1.64 -3.41 -1.52 -3.64 1.76 -1.32 -3.63 -1.62 -0.32 1.89
Nuclear Periphery -1.24 -5.80 -6.58 -6.35 -4.04 3.39 -15.24 -18.09 -20.17 -2.62 -0.55 -16.17 -19.19 -17.76 -4.95
Nuclear Periphery Foci 0.48 -3.27 -2.59 -2.64 -2.16 1.02 -2.73 -5.00 -4.92 1.38 -0.84 -5.59 -4.14 -3.73 1.10
Nucleolus -1.37 -0.32 1.15 5.92 3.46 -2.25 -0.85 3.66 5.13 4.24 -3.60 -1.47 3.65 9.03 6.86
Nucleus -0.64 -11.05 -3.76 -5.02 7.68 8.88 -43.80 -25.26 -31.67 19.38 4.68 -50.91 -25.39 -29.88 18.61
Peroxisomes -9.93 0.39 -0.66 5.85 -0.88 -11.76 6.16 1.10 11.33 -2.86 -13.84 5.68 1.12 12.60 -2.44
Vacuole -4.82 -1.51 -13.32 -12.36 -13.33 -3.72 -2.64 -21.09 -18.02 -17.56 -6.92 -1.23 -23.49 -20.05 -23.03
Vacuole Periphery -3.71 -0.63 2.52 6.00 2.87 -5.56 8.35 0.87 4.62 -2.25 -5.76 2.14 1.10 5.06 -0.41
Standard name
Human Ortholog
Description Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss
Localization
Cell Percentages cytoplasm (12%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Aim23

Aim23


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim23-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available