ORF | |
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Human Ortholog | |
Description | Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 3 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 3 | 0 | 0 | 0 | – | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 3 | 1 | 2 | 8 | – | 12 | 1 | 9 | 6 | 16 | 0 | 2 | 8 | 8 | 15 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 274 | 114 | 297 | 246 | 292 | 309 | – | 346 | 199 | 266 | 217 | 286 | 213 | 367 | 321 | 168 | 174 | 161 | 261 | 121 | 275 | 173 | 131 | 190 |
Endoplasmic Reticulum | 2 | 1 | 2 | 0 | 3 | 2 | – | 4 | 6 | 3 | 9 | 10 | 2 | 5 | 7 | 1 | 6 | 2 | 0 | 0 | 2 | 1 | 1 | 0 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 2 | 8 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 1 | 6 | 1 |
Mitochondria | 2 | 0 | 2 | 0 | 6 | 16 | – | 19 | 17 | 35 | 40 | 67 | 2 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 3 | 1 | 0 | 0 |
Nucleus | 1 | 0 | 0 | 5 | 4 | 2 | – | 5 | 6 | 6 | 12 | 12 | 3 | 5 | 9 | 0 | 2 | 3 | 4 | 0 | 4 | 7 | 4 | 5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 2 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 1 | 2 | 14 | 11 | – | 49 | 15 | 42 | 22 | 14 | 0 | 2 | 3 | 52 | 89 | 55 | 4 | 1 | 2 | 7 | 5 | 8 |
Unique Cell Count | 280 | 114 | 302 | 250 | 317 | 333 | 416 | 240 | 344 | 285 | 378 | 218 | 377 | 335 | 198 | 233 | 206 | 278 | 129 | 296 | 201 | 163 | 212 | |
Labelled Cell Count | 282 | 115 | 308 | 254 | 323 | 348 | 442 | 248 | 363 | 306 | 409 | 222 | 382 | 351 | 230 | 289 | 233 | 278 | 129 | 296 | 201 | 163 | 212 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.7 | 22.1 | 14.2 | 19.9 | 17.4 | 14.5 | – | 13.4 | 12.3 | 12.1 | 11.8 | 11.3 | 22.8 | 24.2 | 22.3 | 19.6 | 20.3 | 18.4 | 21.7 | 23.9 | 25.4 |
Std Deviation (1e-4) | 3.7 | 4.0 | 3.7 | 4.6 | 4.2 | 3.7 | – | 3.1 | 3.3 | 3.0 | 3.1 | 3.0 | 6.0 | 4.9 | 5.1 | 4.9 | 8.6 | 5.5 | 5.4 | 6.2 | 5.9 |
Intensity Change (Log2) | – | – | – | 0.48 | 0.29 | 0.02 | – | -0.09 | -0.22 | -0.23 | -0.27 | -0.33 | 0.68 | 0.77 | 0.65 | 0.46 | 0.51 | 0.37 | 0.61 | 0.75 | 0.83 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 1.4 | – | 1.7 | 0 | 1.5 | 0 | 2.5 | 0 | 0 | 1.3 | 0 | 3.5 | 0 |
Cytoplasm | 0.1 | -3.6 | -3.3 | – | -6.6 | -6.4 | -8.0 | -8.1 | -8.4 | -0.5 | -0.9 | -1.9 | -5.8 | -8.4 | -7.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.2 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.1 | – | 3.1 | 4.0 | 5.2 | 6.3 | 7.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.6 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.3 | 2.7 | – | 5.9 | 4.0 | 6.0 | 4.6 | 3.0 | 0 | 0 | 0 | 9.2 | 11.6 | 9.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 22.1552 | 26.0264 | 22.8839 | 20.9844 | 20.5206 | 23.6404 | 20.5747 | 24.936 | 22.8039 | 19.2732 | 20.5022 | 22.6053 | 25.0168 | 29.5186 | 26.5773 | 25.3588 | 27.8843 | 26.7408 |
Actin | 0.0047 | 0.0005 | 0.0092 | 0.0175 | 0.0309 | 0.0037 | 0.0221 | 0.0007 | 0.0116 | 0.0436 | 0.0041 | 0.0054 | 0.0007 | 0.0005 | 0.0005 | 0.0236 | 0.0007 | 0.0015 |
Bud | 0.0005 | 0.0001 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0008 | 0.0001 | 0.0006 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0009 | 0.0002 | 0.0045 | 0.0015 | 0.0011 | 0.0009 | 0.0032 | 0.0002 | 0.0006 | 0.0021 | 0.0005 | 0.001 | 0.0003 | 0.0003 | 0.0005 | 0.0006 | 0.0008 | 0.0014 |
Bud Periphery | 0.0004 | 0.0001 | 0.0005 | 0.0008 | 0.0002 | 0.0004 | 0.0007 | 0.0001 | 0.0004 | 0.0008 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Site | 0.0005 | 0.0001 | 0.0026 | 0.0027 | 0.0005 | 0.0003 | 0.0041 | 0.0001 | 0.0012 | 0.0022 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.9101 | 0.9816 | 0.9 | 0.8641 | 0.869 | 0.9213 | 0.8479 | 0.9845 | 0.9631 | 0.8066 | 0.9343 | 0.9465 | 0.9486 | 0.991 | 0.9783 | 0.9415 | 0.9855 | 0.9569 |
Cytoplasmic Foci | 0.0039 | 0.0007 | 0.0082 | 0.0051 | 0.0035 | 0.003 | 0.0236 | 0.0012 | 0.0017 | 0.0133 | 0.0011 | 0.0037 | 0.0008 | 0.0004 | 0.0004 | 0.0035 | 0.0004 | 0.0015 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0036 | 0.0004 | 0.0027 | 0.0047 | 0.0044 | 0.0014 | 0.0049 | 0.0014 | 0.0008 | 0.0122 | 0.0014 | 0.0021 | 0.0021 | 0.0007 | 0.0009 | 0.0007 | 0.0004 | 0.0012 |
Endosome | 0.0082 | 0.0019 | 0.0134 | 0.0254 | 0.0153 | 0.007 | 0.0154 | 0.0014 | 0.0005 | 0.0324 | 0.0083 | 0.0025 | 0.0022 | 0.0001 | 0.0001 | 0.0033 | 0.0001 | 0.0015 |
Golgi | 0.001 | 0.0003 | 0.0047 | 0.0039 | 0.0063 | 0.0042 | 0.0071 | 0.0002 | 0.0006 | 0.0263 | 0.0077 | 0.0028 | 0.0001 | 0 | 0 | 0.0032 | 0 | 0.0003 |
Lipid Particles | 0.0014 | 0.0002 | 0.0049 | 0.0027 | 0.0055 | 0.0038 | 0.0089 | 0.0004 | 0.0003 | 0.021 | 0.0151 | 0.0022 | 0 | 0 | 0 | 0.0043 | 0 | 0.0008 |
Mitochondria | 0.0019 | 0.0035 | 0.0075 | 0.0078 | 0.0016 | 0.0085 | 0.0053 | 0.0003 | 0.0029 | 0.0082 | 0.0049 | 0.0007 | 0.0004 | 0 | 0 | 0.0007 | 0.0001 | 0.0001 |
None | 0.0032 | 0.0007 | 0.0051 | 0.0026 | 0.0037 | 0.0006 | 0.0055 | 0.0015 | 0.0006 | 0.0007 | 0.0004 | 0.0009 | 0.0017 | 0.0003 | 0.0004 | 0.001 | 0.0005 | 0.0006 |
Nuclear Periphery | 0.016 | 0.0008 | 0.0107 | 0.0096 | 0.0237 | 0.0064 | 0.0078 | 0.001 | 0.0013 | 0.0047 | 0.0046 | 0.0036 | 0.01 | 0.0011 | 0.002 | 0.0021 | 0.0013 | 0.0065 |
Nucleolus | 0.001 | 0.0001 | 0.0011 | 0.0014 | 0.0005 | 0.0004 | 0.0004 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0002 |
Nucleus | 0.0331 | 0.0053 | 0.0143 | 0.0281 | 0.024 | 0.0287 | 0.0239 | 0.0054 | 0.0109 | 0.0138 | 0.0141 | 0.0237 | 0.0304 | 0.0053 | 0.0163 | 0.009 | 0.0096 | 0.0204 |
Peroxisomes | 0.0004 | 0.0001 | 0.003 | 0.0008 | 0.0011 | 0.0019 | 0.0082 | 0 | 0.0018 | 0.0042 | 0.0003 | 0.0033 | 0 | 0 | 0 | 0.0031 | 0 | 0.0002 |
Punctate Nuclear | 0.0057 | 0.0002 | 0.0016 | 0.0019 | 0.0059 | 0.0032 | 0.0057 | 0.0002 | 0.0002 | 0.001 | 0.0008 | 0.0009 | 0.0014 | 0.0002 | 0.0003 | 0.0024 | 0.0005 | 0.0062 |
Vacuole | 0.0023 | 0.0022 | 0.0036 | 0.0127 | 0.0012 | 0.0026 | 0.0025 | 0.0009 | 0.0003 | 0.0037 | 0.0006 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.001 | 0.0011 | 0.0017 | 0.0051 | 0.001 | 0.0015 | 0.0013 | 0.0003 | 0.0001 | 0.0017 | 0.0013 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0.0002 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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