Standard name
Human Ortholog
Description Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; contains SANT domain that is required for SWI/SNF assembly; is essential for displacement of histone H2A-H2B dimers during ATP-dependent remodeling; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0.05 0 0.1 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0.06 0 0.07 – 0 0 0 0 0 0.06 0.1 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.08 0 0 0 0.21 0.15 – 0.24 0.26 0.3 0.08 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.91 0.89 0.94 0.89 0.88 0.83 0.82 – 0.76 0.78 0.72 0.78 0.85 0.85 0.82 – – – 0.82 0.8 0.81 0.74 0.66 0.68
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.14 0.12 0.05 0 0.06 0.2 0.15 – 0.38 0.25 0.38 0.35 0.26 0.2 0.2 – – – 0.12 0.08 0.09 0.16 0.19 0.18
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0.05 0.05 0.05 0.09 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 2 0
Bud 0 0 0 0 0 2 1 – 0 0 0 1 0 0 0 – – – 1 3 4 1 2 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 – – – 0 1 0 0 2 4
Bud Site 0 0 0 0 0 2 3 – 9 5 18 4 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 4 2 8 12 13 8 13 – 4 8 3 8 16 26 52 – – – 1 1 0 0 0 0
Endoplasmic Reticulum 0 1 1 1 1 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 1
Endosome 1 4 0 1 0 0 0 – 0 0 0 0 1 6 1 – – – 3 7 2 1 3 4
Golgi 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 – – – 1 2 1 1 2 0
Mitochondria 11 30 11 4 8 37 30 – 46 54 55 19 20 5 9 – – – 1 3 2 1 0 2
Nucleus 278 329 246 138 192 149 164 – 143 164 131 187 399 387 428 – – – 260 333 219 176 249 221
Nuclear Periphery 2 0 2 0 2 1 0 – 0 0 0 0 3 7 1 – – – 0 1 1 0 0 1
Nucleolus 43 46 12 6 14 35 31 – 72 52 69 85 124 90 105 – – – 38 33 23 38 71 60
Peroxisomes 1 0 0 0 0 0 0 – 0 0 0 0 0 3 2 – – – 0 0 0 0 0 0
SpindlePole 1 1 19 3 6 4 1 – 2 1 3 6 6 7 1 – – – 2 6 1 2 4 3
Vac/Vac Membrane 0 7 0 1 2 0 0 – 1 0 0 0 3 2 9 – – – 8 20 13 11 32 21
Unique Cell Count 305 369 263 155 218 179 200 188 209 182 240 471 457 525 319 418 272 237 376 327
Labelled Cell Count 341 420 299 167 238 238 243 277 284 279 312 572 533 608 319 418 272 237 376 327


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 6.5 6.2 6.5 6.8 5.2 5.8 – 5.3 5.1 4.9 5.7 6.5 6.7 6.5 – – – 5.9 5.9 6.3
Std Deviation (1e-4) 0.9 1.4 1.1 1.4 1.6 1.2 1.3 – 1.4 1.3 1.1 1.3 1.4 1.7 1.5 – – – 1.5 1.6 1.6
Intensity Change (Log2) – – – 0.06 0.13 -0.24 -0.09 – -0.21 -0.29 -0.34 -0.12 0.07 0.11 0.07 – – – -0.07 -0.07 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Cytoplasm 2.2 1.6 0.8 1.8 – -0.6 0.5 0 0.2 0.3 1.6 3.4 – – –
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Mitochondria -0.9 -0.3 5.5 4.1 – 6.4 6.8 0 1.8 0 -2.7 -2.1 – – –
Nucleus -1.6 -2.1 -3.4 -3.9 – -5.3 -4.8 -6.2 -5.1 -3.5 -3.5 -4.5 – – –
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Nucleolus -0.3 0.9 5.0 4.0 – 9.1 6.4 0 8.8 7.3 5.6 5.7 – – –
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 – – –
SpindlePole -2.3 -2.2 -2.3 -3.5 – -3.1 -3.6 0 -2.4 -4.3 -3.9 -5.9 – – –
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.324 5.3544 5.2248 5.2824 4.228 4.8902 4.514 4.333 4.0731 3.4398 3.5951 3.583 3.882 3.4877 3.2434 3.3764 3.4342 3.2067
Actin 0.0412 0 0.0119 0.0027 0.0001 0.0033 0.0302 0.0021 0.0109 0.0051 0.0272 0.0004 0.0229 0 0.0006 0.0029 0.0071 0.0004
Bud 0.0017 0 0.0003 0.0005 0.0001 0.0001 0.0006 0.0002 0.0006 0.0003 0.0007 0.0001 0.0008 0 0.0001 0.0002 0.0003 0
Bud Neck 0.0066 0.0001 0.0041 0.0008 0.0005 0.001 0.0012 0.0007 0.0003 0.0003 0.0011 0.0005 0.0019 0 0.0002 0.0004 0.0007 0.0006
Bud Periphery 0.0038 0 0.0005 0.0034 0.0003 0.0003 0.0008 0.0002 0.0006 0.0005 0.0011 0.0002 0.0021 0 0.0004 0.0005 0.0009 0.0001
Bud Site 0.0128 0.0001 0.0075 0.0021 0.0001 0.0001 0.0039 0.0078 0.0016 0.001 0.0012 0.0002 0.0019 0 0.0006 0.0006 0.0009 0.0001
Cell Periphery 0.0018 0 0.0003 0.0018 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0006 0 0.0001 0.0001 0.0003 0
Cytoplasm 0.0021 0 0.0042 0.0013 0.0001 0.0002 0.005 0 0.0036 0.0021 0.0012 0.0002 0.0133 0 0.0006 0.0003 0.0005 0
Cytoplasmic Foci 0.0209 0 0.0128 0.0034 0 0.0007 0.0179 0.001 0.005 0.0236 0.0237 0.0003 0.0174 0 0.0038 0.0011 0.0127 0.002
Eisosomes 0.0009 0 0.0002 0.0003 0 0 0.0004 0.0001 0.0001 0.0001 0.0001 0 0.0006 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0043 0 0.0037 0.0011 0.0001 0.0004 0.0045 0 0.0046 0.0023 0.0024 0.0005 0.0081 0 0.0005 0.0005 0.0005 0
Endosome 0.0141 0 0.0123 0.0117 0.0001 0.0014 0.0386 0.0001 0.0107 0.0159 0.0327 0.0048 0.0448 0 0.0143 0.0122 0.0097 0.0009
Golgi 0.0076 0 0.0058 0.0013 0 0.0028 0.0268 0.0003 0.0042 0.0108 0.0162 0.0009 0.0098 0 0.0047 0.0075 0.0038 0.0012
Lipid Particles 0.0204 0 0.0121 0.0127 0 0.0023 0.0209 0.0005 0.0119 0.0336 0.0083 0.0024 0.0257 0 0.0063 0.0157 0.0289 0.0054
Mitochondria 0.0086 0.0001 0.0169 0.0332 0.0016 0.0057 0.0235 0.0005 0.0017 0.0058 0.0153 0.0025 0.0259 0 0.0141 0.0036 0.0099 0.0003
None 0.0043 0 0.0032 0.0005 0.0001 0.0002 0.0016 0 0.0007 0.0028 0.0003 0.0001 0.008 0 0.0002 0.0002 0.0008 0
Nuclear Periphery 0.0328 0.0008 0.0088 0.0025 0.0033 0.0045 0.0351 0.0001 0.011 0.0193 0.0078 0.0031 0.0261 0.0011 0.0044 0.0018 0.0109 0.0002
Nucleolus 0.0543 0.0473 0.0226 0.0288 0.1991 0.1346 0.0486 0.0383 0.0453 0.0513 0.1258 0.1405 0.0546 0.0251 0.0256 0.0304 0.2312 0.1239
Nucleus 0.7091 0.9513 0.8538 0.8673 0.7856 0.835 0.7152 0.9462 0.8801 0.7887 0.6912 0.8413 0.7046 0.9733 0.9119 0.9149 0.6391 0.8604
Peroxisomes 0.0247 0 0.0091 0.0031 0 0.0032 0.004 0.0011 0.0015 0.0085 0.0104 0.0001 0.0052 0 0.0062 0.0006 0.0176 0.0023
Punctate Nuclear 0.0139 0.0002 0.0072 0.0012 0.008 0.0028 0.015 0.0005 0.004 0.0239 0.0301 0.0005 0.0066 0.0004 0.0031 0.0011 0.0176 0.0018
Vacuole 0.0098 0 0.0014 0.0163 0.0004 0.0004 0.0031 0.0001 0.001 0.0017 0.0017 0.0005 0.0095 0 0.0013 0.0041 0.0029 0.0001
Vacuole Periphery 0.0041 0 0.0013 0.0039 0.0006 0.0008 0.0028 0 0.0006 0.0023 0.0016 0.001 0.0095 0 0.0011 0.0012 0.0035 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.8338 31.8443 19.1992 24.8028 28.5776 29.3722 30.5878 28.5223 26.3029 32.9533
Translational Efficiency 1.5351 1.316 1.5031 1.0898 1.4101 1.3512 1.0998 0.9842 1.1102 1.1624

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1667 2352 187 179 1554 1077 1733 3026 3221 3429 1920 3205

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 676.90 903.49 1095.09 1172.46 651.76 838.71 971.01 1179.66 664.77 883.14 983.09 1179.26
Standard Deviation 72.04 114.56 126.87 152.89 111.12 117.63 120.87 177.77 93.81 119.38 126.92 176.48
Intensity Change Log 2 — 0.416566 0.694035 0.792524 — 0.363831 0.575145 0.855958 — 0.390938 0.636938 0.823990

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001081 0.000503 0.001036 0.001495 0.001019 0.001748 0.000692 0.000574 0.001051 0.000894 0.000726 0.000625
Bud Neck 0.004729 0.005926 0.011816 0.012065 0.004056 0.006481 0.012804 0.030640 0.004404 0.006100 0.012708 0.029602
Bud Site 0.003772 0.000939 0.001037 0.001347 0.006954 0.003324 0.001012 0.001326 0.005307 0.001688 0.001014 0.001327
Cell Periphery 0.000276 0.000053 0.000029 0.000056 0.000557 0.000142 0.000042 0.000047 0.000412 0.000081 0.000041 0.000048
Cytoplasm 0.016353 0.000570 0.000047 0.000534 0.022333 0.005966 0.000417 0.000218 0.019238 0.002265 0.000381 0.000235
Cytoplasmic Foci 0.006200 0.000200 0.000024 0.000033 0.005245 0.001170 0.000252 0.000099 0.005739 0.000504 0.000229 0.000096
Eisosomes 0.000060 0.000047 0.000061 0.000062 0.000056 0.000067 0.000051 0.000041 0.000058 0.000053 0.000052 0.000042
Endoplasmic Reticulum 0.005674 0.003179 0.001587 0.002561 0.005203 0.006299 0.002502 0.002182 0.005447 0.004159 0.002413 0.002203
Endosome 0.004077 0.000310 0.000054 0.000086 0.002915 0.001088 0.000341 0.000341 0.003517 0.000554 0.000313 0.000327
Golgi 0.007569 0.000546 0.000126 0.000149 0.003807 0.002467 0.000265 0.000173 0.005754 0.001149 0.000251 0.000172
Lipid Particles 0.002470 0.000077 0.000045 0.000052 0.000632 0.001175 0.000071 0.000029 0.001583 0.000422 0.000069 0.000031
Mitochondria 0.012279 0.003888 0.001953 0.001926 0.003255 0.008189 0.001470 0.002732 0.007925 0.005239 0.001517 0.002687
Mitotic Spindle 0.003636 0.002295 0.000340 0.003923 0.002332 0.005145 0.003089 0.003716 0.003007 0.003190 0.002821 0.003727
None 0.036470 0.002030 0.000760 0.002471 0.077818 0.008971 0.001951 0.000647 0.056419 0.004210 0.001835 0.000749
Nuclear Periphery 0.001119 0.000348 0.000086 0.000228 0.000171 0.001040 0.000310 0.001218 0.000661 0.000565 0.000288 0.001163
Nuclear Periphery Foci 0.005256 0.000825 0.000043 0.000102 0.004211 0.002242 0.000249 0.000341 0.004752 0.001270 0.000229 0.000328
Nucleolus 0.160663 0.126337 0.146288 0.071556 0.154200 0.125977 0.093311 0.041683 0.157545 0.126224 0.098471 0.043351
Nucleus 0.718013* 0.848035* 0.830839* 0.898395* 0.697282* 0.810579* 0.879233* 0.911701* 0.708011* 0.836271* 0.874519* 0.910957*
Peroxisomes 0.004065 0.000690 0.002990 0.002088 0.003112 0.003335 0.000745 0.000194 0.003605 0.001521 0.000963 0.000300
Vacuole 0.004448 0.001693 0.000595 0.000689 0.003698 0.002901 0.000679 0.001117 0.004086 0.002072 0.000671 0.001093
Vacuole Periphery 0.001791 0.001510 0.000245 0.000182 0.001145 0.001695 0.000516 0.000979 0.001480 0.001568 0.000489 0.000935

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.49 0.25 -3.81 -10.53 -4.96 -3.94 3.67 4.82 6.67 2.97 1.97 3.91 4.61 3.42 1.92
Bud Neck -2.73 -5.18 -6.99 -6.36 -1.32 -3.25 -11.60 -22.58 -20.07 -14.55 -4.34 -13.33 -23.68 -22.34 -14.61
Bud Site 7.29 5.54 3.11 -4.29 -2.53 3.45 7.33 6.89 2.62 -1.65 7.59 9.46 8.64 0.76 -1.88
Cell Periphery 7.57 8.16 6.92 -1.09 -2.49 8.45 10.90 10.89 6.36 -0.53 11.84 13.31 13.33 3.97 -0.83
Cytoplasm 9.58 9.93 9.60 0.17 -2.86 6.86 11.17 11.34 4.30 0.73 12.68 14.68 14.97 4.66 0.57
Cytoplasmic Foci 8.01 8.24 8.21 3.06 -1.92 5.36 6.54 6.78 4.77 0.90 9.79 10.14 10.55 4.51 0.87
Eisosomes 5.39 -0.27 -1.40 -5.67 -0.79 -2.13 2.48 5.93 8.59 5.61 3.16 3.69 8.47 6.65 5.67
Endoplasmic Reticulum 6.92 10.39 5.95 -0.34 -4.67 -2.44 8.56 10.21 10.50 2.24 4.65 12.25 14.06 10.04 1.42
Endosome 5.63 6.00 5.83 1.78 -3.22 2.70 3.91 3.95 2.85 0.09 6.24 6.65 6.69 1.76 -0.01
Golgi 8.38 8.93 8.81 2.79 -3.95 1.90 6.73 7.02 5.16 0.61 8.81 10.80 11.10 5.80 0.55
Lipid Particles 6.04 6.10 5.96 -1.09 -2.09 -0.91 7.14 8.47 2.05 1.05 4.29 7.24 7.49 2.20 0.98
Mitochondria 5.43 6.62 7.16 2.38 -0.32 -3.58 4.34 0.64 3.81 -3.56 2.76 8.31 6.17 3.34 -3.52
Mitotic Spindle 1.52 4.38 -1.31 -1.53 -1.82 -1.90 -0.51 -1.35 0.86 -0.76 -0.27 0.27 -1.34 -1.14 -1.35
None 14.55 15.08 14.05 -0.73 -2.95 15.46 17.65 18.00 6.92 2.91 21.28* 22.32 22.96 8.51 2.66
Nuclear Periphery 2.54 3.61 2.41 -0.53 -4.50 -2.11 -1.58 -5.23 -0.48 -3.94 0.41 1.94 -2.30 -2.61 -4.10
Nuclear Periphery Foci 6.79 8.44 7.85 1.65 -2.02 2.54 7.55 7.48 3.71 -0.35 7.49 10.88 10.73 3.74 -0.54
Nucleolus 7.32 1.65 7.16 3.99 3.99 5.50 12.22 26.02* 19.96 13.47 9.17 14.30 33.22* 28.37* 14.55
Nucleus -18.62 -9.21 -11.01 -1.43 -2.14 -12.91 -23.54 -28.18 -14.97 -5.80 -23.97 -28.82 -36.16 -17.05 -6.59
Peroxisomes 7.09 2.00 3.75 -6.10 1.58 0.05 5.82 7.01 8.46 10.08 6.45 8.36 10.32 9.72 9.43
Vacuole 3.78 5.64 4.67 0.98 -1.89 0.87 6.77 5.75 2.36 -1.12 3.90 8.03 6.85 2.50 -1.26
Vacuole Periphery 0.85 7.75 8.24 5.32 0.60 -1.66 2.95 0.95 2.13 -1.53 -0.46 5.13 2.66 2.58 -1.59
Standard name
Human Ortholog
Description Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; contains SANT domain that is required for SWI/SNF assembly; is essential for displacement of histone H2A-H2B dimers during ATP-dependent remodeling; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions
Localization
Cell Percentages nucleus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Swi3

Swi3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swi3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available