Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L17B; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0.08 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0 0.05 0.05 0.07 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.97 0.93 0.84 0.79 0.72 0.66 0.62 0.65 0.6 0.98 0.98 0.98 0.99 0.98 0.98 0.99 0.96 0.92 0.91 0.88 0.83
Endoplasmic Reticulum 0 0 0 0 0 0.12 0.16 0.16 0.17 0.22 0.17 0.22 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.05 0.06 0.08 0.09 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.11 0.05 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 2 4 7 13 16 38 33 38 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 0 0 0 1 1 0 0 0 2 1 0 0 0 0 0 0 0 0 2 8 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 6 9 8 5 17 12 17 15 34 25 40 1 5 3 0 0 1 0 0 2 0 0 1
Cytoplasm 203 622 558 294 371 275 315 273 185 283 238 284 278 376 334 161 111 135 189 597 529 192 252 238
Endoplasmic Reticulum 2 8 14 10 16 41 65 59 47 102 62 103 8 14 1 3 6 4 1 5 11 1 12 11
Endosome 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 2 0 1 1 10 20 18 36 33 49 0 0 0 0 0 1 0 0 0 0 0 1
Mitochondria 0 0 0 0 1 1 4 0 31 25 28 37 0 0 0 4 1 1 0 1 3 0 1 0
Nucleus 0 0 1 0 3 0 0 0 0 1 0 0 0 0 2 0 0 0 0 2 0 3 1 2
Nuclear Periphery 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 2 2
Nucleolus 0 0 0 0 1 0 0 0 0 3 1 0 0 0 3 1 0 0 0 0 0 3 5 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 1 1 4
Vac/Vac Membrane 0 1 5 0 12 3 12 21 9 8 7 15 1 2 1 0 2 2 0 8 1 1 4 6
Unique Cell Count 205 629 573 304 397 328 397 377 282 457 367 475 285 384 340 163 113 138 192 622 573 211 286 286
Labelled Cell Count 211 638 590 312 413 343 425 403 321 534 430 566 288 397 346 169 120 144 192 622 573 211 286 286


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 78.4 59.7 59.9 53.3 31.9 32.3 26.9 24.8 22.2 22.8 22.3 20.9 56.9 53.5 49.9 75.6 95.8 82.6 61.8 64.6 63.5
Std Deviation (1e-4) 13.3 12.7 14.9 10.7 9.5 6.7 5.8 5.6 6.4 5.6 5.4 4.4 14.3 10.7 11.4 27.8 27.8 28.8 20.0 20.4 23.0
Intensity Change (Log2) – – – -0.17 -0.91 -0.89 -1.16 -1.27 -1.44 -1.4 -1.43 -1.52 -0.07 -0.16 -0.27 0.33 0.68 0.46 0.04 0.11 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 4.5 5.8 7.0 7.3 6.9 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.1 -0.4 3.1 1.5 2.7 3.1 4.7 4.2 5.2 0 -0.3 0 0 0 0
Cytoplasm -0.6 -3.0 -7.4 -9.2 -11.4 -12.9 -14.6 -13.5 -15.2 0.1 0.5 0.8 1.0 0.5 0.3
Endoplasmic Reticulum 0.7 1.4 6.1 7.8 7.5 7.6 10.0 7.9 9.8 0.3 1.1 -2.5 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 5.0 5.5 6.4 6.8 7.5 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 8.1 5.7 6.7 6.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.5 0 2.5 4.3 0 1.3 0 2.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 64.0318 75.6805 71.222 74.099 69.4882 66.282 55.5708 79.6261 74.0223 71.3023 70.4472 74.7324 57.5503 70.8526 63.426 66.0915 62.6857 66.3153
Actin 0.0064 0.0007 0.0018 0.0109 0.0009 0.0022 0.0215 0.0003 0.0055 0.0001 0.0053 0.002 0.0095 0.0002 0.0018 0.0002 0.0069 0.0358
Bud 0.0015 0.0008 0.0014 0.0012 0.0008 0.0021 0.0034 0.0004 0.0018 0.0011 0.0014 0.002 0.0009 0.0007 0.0008 0.0006 0.0004 0.0004
Bud Neck 0.0013 0.0008 0.0013 0.0011 0.0012 0.0042 0.0008 0.0003 0.0005 0.0004 0.0005 0.0008 0.0007 0.0003 0.0008 0.0007 0.0006 0.0019
Bud Periphery 0.0019 0.0004 0.0011 0.0007 0.001 0.0025 0.0066 0.0001 0.0012 0.0004 0.0024 0.002 0.0019 0.0021 0.0008 0.0002 0.0002 0.0001
Bud Site 0.0022 0.0016 0.001 0.0004 0.0009 0.0004 0.0016 0.0002 0.0009 0.0002 0.0002 0.0002 0.0005 0.0003 0.0017 0.0001 0.0002 0.0001
Cell Periphery 0.0124 0.0145 0.0049 0.0033 0.0059 0.0028 0.0039 0.0013 0.0036 0.0427 0.0009 0.0008 0.0063 0.0014 0.0086 0.0006 0.0008 0.0002
Cytoplasm 0.7011 0.8961 0.8435 0.7181 0.7391 0.7828 0.616 0.9737 0.8682 0.7179 0.8433 0.8386 0.7509 0.9662 0.854 0.8433 0.8411 0.8274
Cytoplasmic Foci 0.0021 0.002 0.0047 0.0018 0.0122 0.0048 0.0114 0.0018 0.0034 0.001 0.0102 0.0073 0.0073 0.0012 0.0061 0.0046 0.008 0.0063
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0.0003 0 0.0003 0 0.0001 0 0.0003 0 0.0004 0 0.0001 0
Endoplasmic Reticulum 0.0565 0.0182 0.0274 0.0071 0.0131 0.0289 0.0553 0.0035 0.021 0.0032 0.0385 0.0553 0.0789 0.0037 0.0219 0.016 0.0051 0.0795
Endosome 0.0126 0.0021 0.0083 0.0101 0.0212 0.0343 0.0353 0.0026 0.012 0.0063 0.0464 0.0319 0.0235 0.0028 0.0152 0.0165 0.0322 0.0204
Golgi 0.0015 0.0004 0.0015 0.0097 0.0022 0.003 0.0053 0.0004 0.0139 0.0001 0.0136 0.0074 0.0036 0.0002 0.0017 0.0007 0.0034 0.0029
Lipid Particles 0.0011 0.0012 0.0014 0.0005 0.0059 0.0006 0.0042 0.0003 0.0011 0.0005 0.0002 0.0002 0.0028 0.0001 0.0017 0.0002 0.0069 0.0006
Mitochondria 0.0044 0.0004 0.0067 0.0027 0.0083 0.0133 0.0435 0.0002 0.0095 0.0002 0.0212 0.0067 0.0046 0.0004 0.0015 0.0003 0.0016 0.0029
None 0.0015 0.0006 0.0009 0.0001 0.0039 0.0007 0.0245 0.0001 0.0016 0.0001 0.0007 0.0004 0.0043 0.0002 0.0097 0.0002 0.0005 0.0005
Nuclear Periphery 0.0258 0.0049 0.0084 0.0082 0.0083 0.0108 0.041 0.0013 0.0054 0.008 0.0023 0.0131 0.0242 0.0011 0.0082 0.0055 0.0164 0.0037
Nucleolus 0.0013 0.0002 0.0007 0.0001 0.003 0.0009 0.0097 0 0.0007 0.0003 0 0 0.0005 0 0.001 0.0001 0.0002 0
Nucleus 0.0089 0.0019 0.0041 0.0032 0.0044 0.0054 0.0179 0.0007 0.0022 0.0026 0.0008 0.0021 0.0066 0.0012 0.0046 0.0019 0.0021 0.0009
Peroxisomes 0.0001 0.0001 0.0002 0.0001 0.0007 0.0002 0.0059 0 0.0002 0 0.0001 0.0001 0.0003 0 0.0004 0 0.0009 0.0007
Punctate Nuclear 0.0002 0.0001 0.0002 0 0.0015 0.0001 0.0027 0 0.0001 0 0.0001 0.0001 0.0026 0 0.0007 0 0.0029 0.0014
Vacuole 0.1371 0.0503 0.0685 0.1895 0.1386 0.0756 0.0579 0.0108 0.0355 0.1687 0.0068 0.0188 0.0531 0.0147 0.0477 0.0938 0.0548 0.009
Vacuole Periphery 0.0199 0.0027 0.0121 0.0311 0.0267 0.0244 0.0312 0.0017 0.0115 0.046 0.0049 0.01 0.0167 0.0031 0.0108 0.0146 0.0146 0.0053

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1306.1923 1388.0357 1479.693 1276.4698 1514.3867 1251.8198 1133.4452 1186.7644 1198.7613 1067.9743
Translational Efficiency 1.3784 1.4404 1.4057 1.6267 1.5915 1.5314 1.5456 1.7079 1.5989 1.7814

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L17B; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl17b

Rpl17b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl17b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available