Standard name
Human Ortholog
Description RING-finger peroxin and E3 ubiquitin ligase; peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0.06 0.06 0 0 0.09 0 0 – 0 0 0 0.26 0.1 0.14 0 0 0 0.05 0.06 0 0 0.09 0.12
Bud – 0.11 0.21 0.1 0.21 0.21 0.19 0.18 – 0.2 0.21 0.17 0.13 0.09 0.1 0.06 0.05 0.12 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0.07 0.07 0.09 0.11 0.08 0.05 – 0.06 0.07 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.08 0.07 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0.08 0.2 0.12 0.11 0.16 0.17 0.05 0.15 0.12 0.19 0.12 0.09
Golgi – 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0
Mitochondria – 0.82 0.65 0.61 0.56 0.74 0.66 0.5 – 0.53 0.65 0.71 0.26 0.16 0.19 0.15 0.1 0.12 0 0.06 0 0 0.1 0.11
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0.33 0.51 0.55 0.5 0.32 0.37 0.41 – 0.38 0.31 0.3 0.38 0.59 0.45 0.55 0.51 0.49 0.82 0.58 0.73 0.6 0.6 0.56
SpindlePole – 0.1 0.24 0.22 0.26 0.15 0.24 0.34 – 0.3 0.24 0.18 0.32 0.42 0.26 0.27 0.29 0.27 0 0.06 0.06 0.08 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 6 13 2 5 40 12 9 – 8 7 27 43 22 34 3 2 4 2 6 4 2 20 37
Bud – 11 45 20 96 93 124 93 – 126 96 117 22 19 26 12 9 17 0 2 1 1 1 6
Bud Neck – 0 3 1 4 7 5 4 – 6 2 1 2 2 3 1 2 3 0 3 0 2 2 6
Bud Site – 3 16 15 43 47 52 29 – 38 31 41 6 3 7 6 3 6 – – – – – –
Cell Periphery – 0 0 0 2 0 1 0 – 0 2 1 2 1 2 0 3 0 0 0 0 0 0 1
Cytoplasm – 1 2 7 9 8 13 8 – 15 7 12 6 7 7 13 15 10 0 0 1 0 2 1
Endoplasmic Reticulum – 2 0 0 0 4 1 0 – 2 0 5 1 1 4 2 3 3 0 0 0 0 0 2
Endosome – 4 3 6 20 3 6 17 – 10 5 6 13 43 31 22 31 23 2 17 26 25 27 28
Golgi – 1 0 0 0 0 0 0 – 0 0 0 9 2 5 7 5 8 0 4 2 0 3 8
Mitochondria – 80 141 127 260 326 432 263 – 332 300 505 43 35 48 30 20 17 0 7 5 5 22 33
Nucleus – 0 0 0 0 0 1 3 – 3 2 4 1 0 1 2 3 1 0 0 0 1 0 1
Nuclear Periphery – 1 0 0 2 6 9 2 – 0 4 9 1 1 2 7 4 3 0 0 0 0 0 0
Nucleolus – 1 1 3 6 6 9 11 – 9 7 10 0 2 1 5 6 4 0 0 0 0 0 0
Peroxisomes – 32 110 114 230 141 240 218 – 238 142 214 63 125 112 108 98 68 40 69 168 79 137 179
SpindlePole – 10 51 45 122 68 155 178 – 191 112 125 53 90 65 53 56 37 1 6 14 10 5 8
Vac/Vac Membrane – 1 0 2 4 1 1 1 – 3 1 3 1 0 4 0 4 0 0 0 0 2 1 1
Unique Cell Count 98 216 209 461 442 651 529 629 461 708 164 213 248 195 194 139 49 120 229 133 228 321
Labelled Cell Count 153 385 342 803 750 1061 836 981 718 1080 266 353 352 271 264 204 49 120 229 133 228 321


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 5.0 3.8 3.7 3.9 3.7 3.1 3.5 – 3.2 3.3 3.3 5.9 5.7 5.8 6.6 7.0 7.0 5.7 5.9 5.9
Std Deviation (1e-4) – 0.7 1.0 0.9 2.9 0.9 0.7 0.8 – 0.9 0.8 0.6 0.9 0.9 0.9 1.4 1.7 1.5 2.2 0.9 1.3
Intensity Change (Log2) – – – -0.06 0.03 -0.03 -0.3 -0.13 – -0.24 -0.2 -0.2 0.62 0.59 0.6 0.78 0.87 0.87 0.58 0.63 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – -1.9 -3.5 -3.1 -4.3 -4.8 -2.1
Bud Neck – – – – – – – – – 0 0 0 0 0 0
Bud Site – – – – – – – – – -1.5 -3.0 -2.3 -1.9 -2.8 -1.2
Cell Periphery – – – – – – – – – 0 0 0 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – 0 0 0 0 0 0
Endosome – – – – – – – – – 3.1 6.3 4.6 4.2 5.3 5.4
Golgi – – – – – – – – – 0 0 0 0 0 0
Mitochondria – – – – – – – – – -7.5 -10.3 -10.0 -10.2 -11.4 -9.8
Nuclear Periphery – – – – – – – – – 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – 0 0 0 0 0 0
Peroxisomes – – – – – – – – – -2.4 1.6 -1.2 0.9 -0.1 -0.4
SpindlePole – – – – – – – – – 1.9 4.1 0.6 0.8 1.2 0.6
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – 5.5 1.6 2.7 -2.3 -2.7 -1.4
Cytoplasm – – – – – – – – – 0 0 0 3.1 3.5 0
Nucleus – – – – – – – – – 0 0 0 0 0 0
Vacuole – – – – – – – – – 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.851 0.6195 0.7074 0.7181 0.1658 0.7577 2.5916 2.76 2.5831 2.3252 2.8955 2.561 0.3433 0.4058 0.2838 0.1441 0.8539 0.2926
Actin 0.0023 0.0219 0.0152 0.0081 0.0005 0.0075 0.0231 0.0124 0.036 0.0192 0.0208 0.0134 0.0232 0.0208 0.0515 0.0166 0.0052 0.0225
Bud 0 0.0023 0.0001 0.0043 0.0001 0 0.0001 0.0008 0.0002 0.0001 0.0001 0.0007 0.0002 0.0005 0.0005 0.0007 0.0002 0.0001
Bud Neck 0.0009 0.0004 0.0047 0.0473 0.0003 0.0002 0.0032 0.0051 0.0006 0.0006 0.0002 0.0004 0.0017 0.0115 0.0102 0.0008 0.0004 0.0124
Bud Periphery 0 0.003 0.0002 0.0126 0.0002 0.0001 0.0001 0.001 0.0003 0.0001 0.0001 0.0009 0.0003 0.0007 0.0006 0.0033 0.0002 0.0001
Bud Site 0.0005 0.0195 0.0064 0.0034 0.0003 0.0003 0.0058 0.0519 0.0017 0.0004 0.0007 0.0002 0.0048 0.015 0.0109 0.0023 0.0004 0.0003
Cell Periphery 0 0.0001 0 0.0002 0 0 0.0001 0.0002 0 0 0 0 0.0002 0 0.0002 0.0001 0.0001 0
Cytoplasm 0.0001 0.0036 0.0001 0.0003 0.0003 0.0002 0.001 0.0013 0.0015 0.0004 0.0003 0.0006 0.0042 0.0004 0.0018 0.0002 0.0013 0.0003
Cytoplasmic Foci 0.0701 0.09 0.0678 0.0649 0.0552 0.0524 0.0669 0.0983 0.045 0.0484 0.0708 0.0533 0.115 0.1789 0.0978 0.088 0.1232 0.0872
Eisosomes 0 0 0 0.0001 0 0 0.0002 0.0007 0.0001 0 0 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0 0 0 0.0001 0 0 0.0002 0.0001 0.0004 0 0 0 0.0014 0 0.0004 0 0.0023 0.0001
Endosome 0.0077 0.0093 0.0033 0.0056 0.03 0.0043 0.0132 0.0159 0.0055 0.038 0.0142 0.0051 0.0264 0.0126 0.0187 0.0027 0.0489 0.0085
Golgi 0.0083 0.0137 0.0069 0.0132 0.0141 0.0076 0.0175 0.0144 0.0096 0.0255 0.009 0.0093 0.0173 0.0167 0.0157 0.0065 0.0351 0.0153
Lipid Particles 0.0555 0.0139 0.0185 0.0132 0.0192 0.0392 0.0434 0.0078 0.0144 0.0338 0.02 0.0369 0.0791 0.0371 0.0267 0.0178 0.1313 0.0755
Mitochondria 0.0087 0.007 0.012 0.0105 0.0034 0.011 0.0073 0.0078 0.0135 0.0083 0.0029 0.005 0.0105 0.0052 0.0082 0.0323 0.0058 0.0133
None 0.0002 0.003 0.0048 0.0011 0.0001 0.0013 0.0067 0.0207 0.001 0.0001 0.0006 0.0037 0.0016 0.0005 0.0052 0.0015 0.0006 0.0017
Nuclear Periphery 0.0001 0.0001 0 0.0005 0 0.0002 0.0005 0.0001 0.0012 0 0 0 0.0044 0 0.0006 0 0.0462 0.0001
Nucleolus 0.0002 0.0002 0.0002 0.0009 0.0001 0.0006 0.0004 0.0005 0.0002 0.0001 0.0001 0.0004 0.0003 0.0002 0.0008 0.0003 0.0005 0.0002
Nucleus 0 0.0002 0.0001 0.0012 0 0.0002 0.0005 0.0002 0.0009 0 0 0.0001 0.001 0 0.0008 0.0001 0.0139 0
Peroxisomes 0.8397 0.8045 0.8549 0.8099 0.8736 0.8679 0.808 0.7472 0.8662 0.8231 0.8589 0.8641 0.6889 0.6983 0.7401 0.8256 0.5777 0.7581
Punctate Nuclear 0.0054 0.0072 0.0046 0.0017 0.0006 0.0067 0.0008 0.0131 0.0013 0.0003 0.0008 0.0053 0.0157 0.0013 0.0084 0.0007 0.0032 0.0039
Vacuole 0.0001 0.0002 0 0.0006 0.0015 0.0001 0.0007 0.0004 0.0002 0.0011 0.0002 0.0001 0.003 0.0001 0.0006 0.0002 0.0016 0.0002
Vacuole Periphery 0.0001 0 0 0.0002 0.0004 0.0001 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0.0009 0.0001 0.0002 0.0001 0.0019 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.2549 20.5495 23.3214 29.2777 17.532 22.9749 18.9443 30.1092 29.9264 23.2206
Translational Efficiency 0.3602 0.4213 0.5123 0.5669 0.7154 0.276 0.4542 0.4156 0.5256 0.498

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RING-finger peroxin and E3 ubiquitin ligase; peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import
Localization
Cell Percentages peroxisome (70%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pex2

Pex2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available