Standard name
Human Ortholog
Description Beta-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.73 0.62 0.48 0.48 0.39 0.42 0.34 0.3 0.36 0.25 0.31 0.33 0.73 0.62 0.56 0.43 0.61 0.5 0.41 0.44 0.56 0.32 0.42 0.41
Bud 0.2 0.23 0.17 0.13 0.21 0.15 0.21 0.19 0.19 0.18 0.19 0.19 0.24 0.16 0.14 0.14 0.14 0.14 0.15 0.14 0.07 0.16 0.19 0.21
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.14 0 0.31 0.29 0.22 0.36 0.23 0.22
Bud Site 0.32 0.3 0.23 0.28 0.24 0.3 0.13 0.11 0.11 0.09 0.09 0.1 0.15 0.07 0.1 0.32 0.22 0.18 – – – – – –
Cell Periphery 0.1 0.1 0.13 0.08 0 0.06 0.12 0.09 0.11 0.1 0.12 0.19 0.17 0.23 0.16 0.09 0.14 0.12 0.08 0 0.08 0.05 0.05 0.07
Cytoplasm 0.06 0.1 0.12 0.11 0.08 0.08 0.05 0.1 0.09 0.07 0.08 0.07 0.16 0.17 0.19 0.11 0.14 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0.09 0.06 0.1 0.07 0.09 0 0.08 0.09 0.07 0.05 0.05 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.19 0.09 0.11 0.14 0.24 0.3 0.31 0.52 0.62 0.62 0.52 0 0 0 0.14 0.13 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0.24 0.25 0.23 0.28 0.25 0.19 0.16 0.1 0.1 0.17 0 0 0 0.09 0.05 0.09 0 0 0 0 0 0
SpindlePole 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.13 0.14 0.11 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 186 222 161 86 130 147 169 158 130 142 128 182 67 104 123 92 112 68 121 192 237 56 106 109
Bud 52 83 58 23 71 53 103 100 69 100 80 104 22 27 30 29 25 19 44 58 29 28 47 55
Bud Neck 11 14 3 7 12 3 3 5 2 6 1 3 0 7 13 14 26 6 90 123 91 63 57 59
Bud Site 83 106 77 49 81 105 63 57 40 51 36 55 14 12 22 69 40 25 – – – – – –
Cell Periphery 26 35 44 14 13 22 59 46 41 57 49 102 16 38 35 19 26 17 23 16 33 8 13 17
Cytoplasm 15 37 39 19 27 28 26 52 31 39 34 36 15 28 41 24 26 22 1 7 2 1 2 3
Endoplasmic Reticulum 12 16 10 8 30 20 48 39 33 23 33 47 6 8 11 5 9 7 1 5 6 4 5 4
Endosome 0 3 9 2 5 5 10 15 1 4 2 2 0 0 1 2 0 0 2 4 3 2 3 2
Golgi 1 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 3 1 2 0 2 0 0
Mitochondria 25 67 31 19 46 84 149 165 185 350 253 285 2 0 0 29 24 8 2 9 5 3 9 4
Nucleus 10 7 8 6 6 10 14 18 3 9 5 8 0 1 7 23 6 3 0 0 0 0 0 0
Nuclear Periphery 1 2 2 1 3 0 9 6 6 5 2 5 0 0 1 0 0 0 1 1 2 0 1 0
Nucleolus 1 1 4 5 4 8 21 33 15 31 22 14 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 12 8 80 44 77 99 124 100 56 58 41 95 2 0 3 19 9 13 1 1 4 0 1 1
SpindlePole 13 3 14 6 5 2 7 16 2 7 3 3 3 12 28 29 20 14 0 0 0 0 0 0
Vac/Vac Membrane 3 4 1 0 7 1 5 16 5 13 1 9 1 0 1 2 1 0 3 5 1 1 1 1
Unique Cell Count 256 356 334 178 333 354 502 528 359 568 411 548 92 167 219 213 185 137 296 433 421 175 254 264
Labelled Cell Count 451 608 542 289 518 587 810 827 619 895 690 950 148 237 316 356 324 205 296 433 421 175 254 264


Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 6.9 7.6 7.6 7.2 6.7 6.5 6.7 6.2 5.7 5.8 6.2 7.6 8.1 8.3 6.4 7.1 8.7 8.8 8.8 9.3
Std Deviation (1e-4) 1.1 1.0 1.5 1.3 1.5 1.5 1.4 1.7 1.4 1.3 1.4 1.3 0.9 0.9 1.0 1.1 1.4 1.7 1.7 1.6 1.5
Intensity Change (Log2) – – – 0.0 -0.08 -0.19 -0.24 -0.19 -0.3 -0.42 -0.38 -0.3 0.0 0.09 0.12 -0.25 -0.11 0.19 0.22 0.21 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -2.4 -1.8 -4.2 -5.4 -3.2 -7.1 -4.8 -4.4 4.2 3.0 1.8 -1.1 2.7 0.3
Bud -1.3 1.3 -0.9 1.1 0.6 0.6 0.1 0.7 0.6 1.4 -0.3 -1.2 -1.2 -1.1 -0.9
Bud Neck 0 2.4 0 0 0 0 0 0 0 0 0 3.5 3.7 6.2 0
Bud Site 1.1 0.4 2.0 -4.0 -4.8 -4.2 -5.8 -5.4 -5.3 -1.6 -4.4 -3.9 2.4 -0.4 -1.1
Cell Periphery -1.8 -4.3 -3.1 -0.6 -2.1 -0.7 -1.4 -0.5 2.1 1.0 2.7 0.9 -1.5 0.3 -0.2
Cytoplasm -0.3 -1.5 -1.7 -3.4 -0.9 -1.3 -2.5 -1.6 -2.6 1.2 1.6 2.3 -0.1 0.8 1.3
Endoplasmic Reticulum 0.9 3.3 1.7 3.7 2.7 3.4 0.8 2.9 3.3 0 1.0 1.2 -0.5 1.1 0
Endosome 0 -1.1 -1.2 -0.7 0.1 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.5 1.8 5.1 7.0 7.5 12.0 15.4 14.6 12.8 -2.3 -4.1 -4.6 1.6 1.3 -1.2
Nucleus 0 -0.5 0.4 0.3 0.8 -1.6 -0.9 -1.2 -1.0 0 0 0.6 4.1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.1 2.5 3.6 2.4 3.2 3.1 1.4 0 0 0 0 0 0
Peroxisomes 0.2 -0.3 1.2 0.2 -1.8 -2.8 -5.5 -5.1 -2.4 -4.7 -6.9 -7.3 -4.5 -5.5 -3.6
SpindlePole -0.5 -2.1 -3.2 -2.5 -0.9 -3.2 -2.8 -3.1 -3.8 0 1.4 3.7 4.0 2.9 2.5
Vacuole 0 0 0 0 2.8 0 2.3 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.2869 4.574 4.1763 4.0019 4.2685 4.5183 4.493 5.1739 5.111 4.4966 4.9033 5.2792 5.37 5.499 5.7711 5.6355 5.4363 5.7619
Actin 0.2158 0.1164 0.1297 0.0686 0.1822 0.2019 0.2612 0.103 0.131 0.0963 0.112 0.2174 0.1833 0.097 0.1444 0.1179 0.136 0.1793
Bud 0.0052 0.0037 0.002 0.0008 0.0035 0.0034 0.0026 0.0025 0.0017 0.0011 0.0026 0.0029 0.0029 0.0046 0.0019 0.0065 0.0017 0.0033
Bud Neck 0.1951 0.0497 0.6373 0.8526 0.7498 0.6886 0.1752 0.0406 0.5693 0.8453 0.6768 0.6907 0.2144 0.0633 0.5898 0.8334 0.7507 0.6912
Bud Periphery 0.0201 0.0086 0.0072 0.0025 0.0263 0.0241 0.0068 0.0031 0.0057 0.0054 0.0232 0.0205 0.0081 0.0093 0.0066 0.0128 0.0158 0.0221
Bud Site 0.3902 0.6803 0.1878 0.0371 0.0123 0.0106 0.3829 0.8038 0.2339 0.014 0.0182 0.0141 0.3976 0.7254 0.2357 0.0087 0.045 0.0119
Cell Periphery 0.0562 0.0209 0.0089 0.0035 0.0184 0.0224 0.0534 0.0198 0.0151 0.0058 0.0457 0.0349 0.0968 0.0137 0.0127 0.0074 0.0364 0.0479
Cytoplasm 0.0623 0.1003 0.0057 0.0016 0.0022 0.0314 0.0432 0.023 0.007 0.0016 0.0093 0.0147 0.0564 0.0649 0.0038 0.0066 0.005 0.03
Cytoplasmic Foci 0.007 0.0032 0.0064 0.0006 0.0004 0.0021 0.0159 0.0006 0.004 0.0036 0.01 0.0006 0.0023 0.0063 0.0006 0.0005 0.0011 0.002
Eisosomes 0.0054 0.0024 0.0014 0.0005 0.002 0.002 0.004 0.0014 0.0038 0.001 0.0016 0.0021 0.023 0.0009 0.0016 0.0016 0.0023 0.0033
Endoplasmic Reticulum 0.0031 0.0022 0.0002 0.0035 0.0002 0.0015 0.005 0.0003 0.0006 0.0003 0.0022 0.0003 0.001 0.0011 0.0001 0.0013 0.0001 0.0015
Endosome 0.0021 0.0006 0.0004 0.0032 0.0003 0.0008 0.0071 0.0001 0.0011 0.0021 0.0113 0.0001 0.0001 0.0068 0.0001 0.0002 0.0001 0.0016
Golgi 0.0027 0.0018 0.0011 0.0005 0.001 0.0015 0.0066 0.0006 0.0026 0.0027 0.0148 0.0006 0.0011 0.0033 0.0005 0.0009 0.0005 0.0036
Lipid Particles 0.0025 0.0011 0.0017 0.0002 0.0002 0.0009 0.0075 0.0002 0.001 0.0057 0.0201 0.0001 0.0007 0.0006 0.0001 0.0002 0.0001 0.0004
Mitochondria 0.0004 0.0002 0.0001 0.0003 0 0.0003 0.0031 0 0.0015 0.0009 0.0101 0 0.0068 0.0001 0 0.0001 0 0.0002
None 0.021 0.0045 0.0054 0.0005 0.0001 0.0055 0.0039 0.0003 0.0157 0.0015 0.0005 0.0002 0.0019 0.001 0.0012 0.0002 0.0004 0.0003
Nuclear Periphery 0.0005 0.0007 0.0003 0.0133 0.0001 0.0004 0.0071 0.0001 0.0001 0.0004 0.0084 0.0001 0.0002 0.0002 0 0.0005 0 0.0001
Nucleolus 0.0014 0.0003 0.0004 0.0009 0.0002 0.0003 0.0009 0 0.0002 0.0001 0.0005 0.0001 0.001 0.0001 0.0001 0.0001 0.0002 0.0001
Nucleus 0.0013 0.0007 0.0005 0.0072 0.0003 0.0004 0.001 0.0003 0.0002 0.0002 0.0008 0.0002 0.0005 0.0002 0.0002 0.0004 0.0004 0.0002
Peroxisomes 0.001 0.001 0.0021 0.0002 0.0001 0.0005 0.0047 0.0002 0.0047 0.0105 0.0283 0.0001 0.0008 0.0005 0.0001 0.0001 0.0001 0.0003
Punctate Nuclear 0.0007 0.0004 0.001 0.0009 0.0001 0.0003 0.0058 0.0001 0.0003 0.0012 0.0011 0.0001 0.0002 0.0003 0.0001 0.0002 0.0037 0.0001
Vacuole 0.0058 0.0011 0.0003 0.0011 0.0004 0.0011 0.0013 0.0003 0.0004 0.0002 0.0016 0.0003 0.0007 0.0003 0.0002 0.0003 0.0002 0.0005
Vacuole Periphery 0.0002 0.0001 0 0.0003 0 0.0001 0.0009 0 0 0.0001 0.0009 0 0.0001 0.0001 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.66 21.1065 22.6228 25.4545 14.966 28.3171 25.846 29.2872 28.0692 29.8473
Translational Efficiency 0.6351 0.6949 0.6423 0.666 0.8566 0.6473 0.7124 0.6852 0.6469 0.6137

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Beta-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex
Localization
Cell Percentages cell periphery (27%), actin (22%), mixed (33%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Apl1

Apl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available