Standard name
Human Ortholog
Description 5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.92 1.0 0.99 0.99 0.94 0.93 0.93 0.81 0.85 0.75 0.79 0.98 0.99 1.0 – 0.95 0.98 0.85 0.86 0.86 0.87 0.87 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0 0 0 0.07 0.05 0 0.27 0.2 0.32 0.29 0 0 0 – 0.06 0.06 0.05 0.06 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – 0 0 1 1 0 1 3 4
Bud 0 0 0 0 0 1 3 3 10 12 8 8 0 0 0 – 0 0 0 0 0 1 5 11
Bud Neck 0 0 6 5 5 13 16 29 14 18 10 11 3 0 1 – 1 0 0 0 0 0 2 1
Bud Site 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 1 4 1 3 5 3 1 1 1 2 0 1 1 – 1 1 0 0 0 1 1 2
Cytoplasm 91 85 190 286 325 519 632 575 443 520 339 455 354 517 533 – 134 80 107 84 162 171 359 386
Endoplasmic Reticulum 1 1 0 0 0 0 0 0 0 0 0 0 3 0 0 – 6 6 1 0 0 1 2 1
Endosome 0 0 0 0 1 0 2 0 0 2 1 0 2 1 0 – 1 0 2 0 3 1 4 6
Golgi 0 0 0 2 0 0 0 0 0 0 0 0 0 1 2 – 0 0 2 0 0 1 0 1
Mitochondria 4 11 0 3 1 36 36 18 149 120 146 166 1 0 0 – 9 5 6 5 8 5 7 12
Nucleus 0 1 0 0 1 2 2 9 6 2 5 5 0 0 0 – 1 0 0 0 0 0 2 2
Nuclear Periphery 0 3 0 0 0 0 0 0 1 1 0 1 0 0 0 – 0 0 0 0 0 1 1 0
Nucleolus 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 – 1 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – 0 1 0 0 0 0 0 0
SpindlePole 0 0 1 0 1 4 15 16 6 12 25 30 0 0 0 – 0 1 0 0 2 2 9 8
Vac/Vac Membrane 1 0 0 1 1 8 8 10 5 5 2 4 2 2 0 – 2 1 1 2 0 1 2 5
Unique Cell Count 96 92 190 289 327 550 683 617 549 609 452 578 361 520 535 141 82 127 98 189 197 413 454
Labelled Cell Count 97 101 198 301 336 588 719 663 636 694 539 684 365 522 537 156 95 127 98 189 197 413 454


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.6 5.1 4.7 5.3 4.1 4.0 4.4 3.5 3.7 3.5 3.5 4.8 4.9 4.7 4.2 5.1 5.5 5.4 5.5 5.5
Std Deviation (1e-4) 0.7 0.8 1.1 1.5 1.6 1.4 1.4 1.3 1.5 1.9 1.3 0.7 0.9 0.9 1.0 1.8 1.9 1.6 1.5 1.4 1.5
Intensity Change (Log2) – – – -0.1 0.07 -0.29 -0.33 -0.21 -0.55 -0.46 -0.52 -0.53 -0.09 -0.05 -0.1 -0.28 0.01 0.13 0.09 0.12 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –