Standard name
Human Ortholog
Description Protein involved in transcription-coupled nucleotide excision repair; repairs UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Cytoplasm 0.13 0.17 0.15 0.13 0.2 0.17 0.18 0.19 0.14 – 0.13 0.13 0.21 0.24 0.28 0.19 0.21 0.21 0.05 0.1 0.07 0.07 0.05 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.19 0.34 0.63 0.28 0.59 0.64 0.66 0.78 – 0.86 0.86 0.12 0.14 0.24 0.1 0 0.09 0 0 0.07 0 0 0.08
Nucleus 0.77 0.46 0.59 0.48 0.47 0.43 0.42 0.37 0.35 – 0.29 0.24 0.61 0.57 0.41 0.56 0.6 0.63 0.58 0.53 0.51 0.54 0.47 0.29
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08
Vac/Vac Membrane 0.08 0.23 0.05 0.07 0.15 0.11 0 0 0 – 0 0.06 0.11 0.1 0.16 0.1 0.14 0.12 0.19 0.18 0.28 0.3 0.32 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 3 1 0 0 1
Bud 0 3 2 1 0 1 3 6 5 – 11 9 0 2 5 0 0 0 2 3 1 1 7 8
Bud Neck 0 0 0 0 0 1 0 3 1 – 1 1 0 0 0 0 0 0 0 3 0 2 3 3
Bud Site 0 0 1 0 0 5 6 8 6 – 9 9 0 0 1 0 0 1 – – – – – –
Cell Periphery 0 14 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 11 0 0 0 0
Cytoplasm 11 34 22 15 45 40 58 78 38 – 33 36 48 69 84 18 24 21 6 24 13 11 13 19
Endoplasmic Reticulum 2 2 0 0 0 0 1 0 0 – 0 0 0 3 2 5 3 5 0 1 0 0 1 3
Endosome 1 5 1 0 11 2 1 3 1 – 0 0 4 0 3 5 2 2 6 3 1 0 1 10
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 2 0 1 1 0 0 1 0
Mitochondria 0 38 51 72 62 142 204 266 211 – 224 234 28 39 72 9 5 9 3 8 12 1 6 25
Nucleus 67 93 88 55 104 103 134 150 94 – 76 64 142 165 121 52 70 63 71 128 95 94 137 94
Nuclear Periphery 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 2 0 – 2 1 1 5 1 1 0 1 2 0 1 1 10 13
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 2 0 3 2 1 6 3 – 4 0 2 2 0 1 2 1 3 6 3 4 8 26
Vac/Vac Membrane 7 46 8 8 32 26 12 18 9 – 7 15 26 29 48 9 16 12 23 42 52 51 93 98
Unique Cell Count 87 201 149 114 220 242 317 402 269 261 271 232 288 296 93 117 100 122 243 187 173 291 322
Labelled Cell Count 90 237 175 151 257 322 420 541 368 367 369 251 314 337 102 125 115 122 243 187 173 291 322


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.3 3.9 4.1 4.2 3.6 3.4 3.5 3.1 – 2.8 2.7 4.3 4.3 4.0 6.0 6.7 6.2 4.9 4.6 4.8
Std Deviation (1e-4) 0.7 0.9 1.2 1.9 1.8 1.0 1.1 1.6 1.3 – 1.1 1.1 1.1 1.1 1.1 1.9 2.6 2.4 1.7 1.3 1.2
Intensity Change (Log2) – – – 0.1 0.13 -0.12 -0.2 -0.12 -0.34 – -0.44 -0.51 0.15 0.16 0.05 0.65 0.8 0.69 0.33 0.24 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.3 2.3 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0533 1.0535 0.6308 1.0297 0.5602 0.7931 1.2074 1.6247 1.5234 1.4767 1.0898 1.5061 1.5687 2.5997 2.0854 1.9361 2.0578 2.1371
Actin 0.0801 0.0005 0.0128 0.0003 0.0053 0.0137 0.0333 0.0013 0.0038 0.0278 0.0012 0.0028 0.0002 0.0003 0.0151 0.0646 0.0002 0.0048
Bud 0.0014 0.0001 0.0005 0 0.0002 0.0001 0.0007 0.0001 0.0006 0.002 0.0005 0.0002 0.0001 0.0001 0.0004 0.0012 0.0001 0.0001
Bud Neck 0.0264 0.0008 0.001 0.0002 0.0069 0.0221 0.0034 0.0007 0.0007 0.0039 0.002 0.0059 0.0004 0.0003 0.0005 0.0005 0.001 0.0028
Bud Periphery 0.0039 0.0001 0.0008 0 0.0005 0.0001 0.001 0.0001 0.0009 0.0032 0.0005 0.0002 0.0001 0.0001 0.0007 0.0015 0.0001 0.0001
Bud Site 0.0054 0.0016 0.0047 0.0001 0.0009 0.0002 0.0023 0.0093 0.0023 0.0157 0.0047 0.0005 0.0004 0.0006 0.0026 0.0011 0.0001 0.0003
Cell Periphery 0.001 0.0001 0.0001 0 0.0002 0.0001 0.0003 0 0.0001 0.0005 0.0001 0 0.0001 0.0001 0.0002 0 0.0001 0
Cytoplasm 0.0641 0.062 0.0872 0.0432 0.0752 0.0647 0.0655 0.0667 0.0571 0.0306 0.1061 0.0271 0.0348 0.0491 0.0293 0.0733 0.0761 0.0334
Cytoplasmic Foci 0.0186 0.0028 0.0074 0.0032 0.0013 0.0104 0.0143 0.0039 0.016 0.0096 0.0041 0.0056 0.001 0.0016 0.0068 0.0064 0.0007 0.0047
Eisosomes 0.0007 0 0.0002 0 0.0001 0.0002 0.0003 0 0 0.0004 0.0001 0 0 0.0001 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0089 0.0037 0.0036 0.0014 0.016 0.0034 0.0107 0.0011 0.0018 0.0015 0.0062 0.0011 0.0021 0.0038 0.0069 0.0018 0.0025 0.0011
Endosome 0.029 0.0027 0.0067 0.001 0.0072 0.006 0.0183 0.0012 0.0165 0.0387 0.0171 0.0028 0.0014 0.0018 0.0096 0.0074 0.0016 0.0072
Golgi 0.0069 0.0001 0.0015 0.0001 0.0004 0.0023 0.0052 0.0001 0.0021 0.0137 0.0008 0.0028 0 0 0.0053 0.0115 0 0.0021
Lipid Particles 0.0178 0.0004 0.0035 0.0003 0.0005 0.002 0.0072 0.0003 0.0026 0.0074 0.0013 0.0053 0.0001 0.0003 0.0026 0.0015 0.0001 0.0009
Mitochondria 0.0062 0.0006 0.0018 0.0003 0.0033 0.0008 0.0088 0.0005 0.0037 0.0048 0.0026 0.0079 0.0016 0.0012 0.0156 0.014 0.0007 0.0006
None 0.0361 0.0713 0.0718 0.1777 0.0319 0.0632 0.0581 0.1169 0.0868 0.0423 0.0387 0.0796 0.1206 0.1416 0.0834 0.1259 0.1813 0.1025
Nuclear Periphery 0.0285 0.0184 0.0208 0.0082 0.0522 0.0132 0.0267 0.0087 0.0127 0.0073 0.0394 0.0284 0.0134 0.0296 0.0166 0.0257 0.0115 0.0057
Nucleolus 0.0108 0.0064 0.0068 0.0035 0.0101 0.0088 0.007 0.0067 0.005 0.0028 0.0103 0.0084 0.0075 0.0084 0.0054 0.0035 0.0091 0.0109
Nucleus 0.579 0.8001 0.7265 0.6803 0.675 0.7351 0.6268 0.7111 0.6447 0.7117 0.5961 0.6737 0.8035 0.7399 0.7647 0.6245 0.7073 0.7963
Peroxisomes 0.0204 0.0001 0.0036 0.0001 0.0003 0.0002 0.0041 0.0003 0.0097 0.002 0.001 0.0109 0 0.0001 0.0044 0.004 0 0.0003
Punctate Nuclear 0.0444 0.0255 0.0364 0.0798 0.1045 0.0519 0.1009 0.0699 0.1187 0.0708 0.1615 0.1352 0.0113 0.0183 0.0268 0.0302 0.0061 0.0255
Vacuole 0.0083 0.0023 0.002 0.0003 0.0052 0.0011 0.0037 0.0009 0.0113 0.0024 0.0043 0.0009 0.0009 0.002 0.0018 0.0005 0.001 0.0006
Vacuole Periphery 0.002 0.0004 0.0006 0.0001 0.003 0.0004 0.0014 0.0002 0.0029 0.0009 0.0013 0.0004 0.0003 0.0006 0.001 0.0005 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.9268 8.7799 7.189 7.5003 7.0257 4.7388 5.6631 5.3718 8.3042 7.1663
Translational Efficiency 0.5989 0.3161 0.5696 0.6249 0.6102 1.0268 0.8303 0.7895 0.5457 0.5322

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in transcription-coupled nucleotide excision repair; repairs UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein
Localization
Cell Percentages nucleus (78%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rad26

Rad26


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad26-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available