Standard name
Human Ortholog
Description ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.98 0.98 0.95 0.96 0.92 0.77 0.78 0.74 0.7 0.68 0.69 0.98 0.99 0.98 0.95 0.95 0.94 0.92 0.97 0.85 0.88 0.94 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.06 0.17 0.33 0.28 0.36 0.43 0.48 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 3 1 3 0 2 0 3 7 6 9 1 8 0 1 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 1 4 5 4 8 6 1 1 3 1 0 3 9 9 2 0 0 0 0 0 0
Cytoplasm 281 284 181 171 260 236 259 285 172 215 99 124 173 293 259 556 385 208 268 282 160 113 201 171
Endoplasmic Reticulum 1 5 2 0 0 0 0 1 0 2 1 3 0 2 2 16 15 9 4 0 7 1 0 4
Endosome 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 6 4 0 2 0 1 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 3 0 0 0 1 1
Mitochondria 9 3 3 12 15 44 112 101 84 131 69 73 2 0 0 16 1 2 1 1 3 2 1 3
Nucleus 2 0 1 3 2 1 3 4 2 2 2 0 1 0 2 7 7 1 0 0 1 0 0 0
Nuclear Periphery 0 0 0 1 0 0 4 2 0 2 0 0 0 0 0 0 0 0 2 0 0 0 1 0
Nucleolus 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 14 0 2 4 8 1 13 26 9 13 4 3 1 2 2 20 22 13 2 2 8 3 2 6
Unique Cell Count 299 289 185 180 271 257 336 366 231 307 145 179 176 297 263 585 404 221 292 290 189 129 215 196
Labelled Cell Count 310 295 192 192 292 287 399 435 280 375 177 215 178 298 268 631 444 236 292 290 189 129 215 196


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 7.0 6.4 6.4 6.7 5.5 4.8 5.0 4.9 4.3 4.6 4.5 8.0 7.9 8.2 8.4 10.0 10.6 7.1 7.9 9.2
Std Deviation (1e-4) 0.8 1.1 1.1 1.3 1.8 1.0 1.1 1.2 1.6 0.9 1.5 1.1 1.2 1.2 1.3 1.5 1.5 1.7 1.1 1.3 1.6
Intensity Change (Log2) – – – 0.01 0.06 -0.22 -0.42 -0.36 -0.38 -0.59 -0.47 -0.53 0.31 0.31 0.35 0.39 0.64 0.73 0.15 0.29 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.5 -1.1 -2.7 -6.3 -6.1 -6.6 -7.5 -7.4 -7.4 0.3 0.7 0.5 -1.6 -1.5 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1.8 1.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.4 2.1 5.2 0 7.4 8.7 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 1.8 3.0 0 2.0 0 0 0 0 0 1.7 2.5 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5417 4.9791 3.9776 3.4746 3.5038 4.2565 2.8119 3.5854 3.624 3.1148 2.6125 3.5059 4.3778 5.1747 4.4829 4.2769 4.1369 4.8825
Actin 0.065 0.0008 0.0117 0.0014 0.001 0.0004 0.0271 0.0106 0.0306 0.0007 0.0034 0.0068 0.0584 0.0004 0.0028 0.0004 0.0229 0.0217
Bud 0.0013 0.0002 0.0003 0.0002 0.0001 0 0.0017 0.0001 0.0002 0.0001 0.0002 0.0002 0.0012 0.0001 0.0002 0.0001 0.0002 0.0002
Bud Neck 0.0024 0.0001 0.0012 0.0004 0.0007 0.0007 0.0013 0.0002 0.0002 0.0001 0.0006 0.0007 0.0018 0.0001 0.0006 0.0003 0.0005 0.0019
Bud Periphery 0.0011 0.0001 0.0004 0.0001 0.0001 0 0.0028 0 0.0001 0 0.0002 0.0001 0.0017 0 0.0001 0 0.0002 0.0002
Bud Site 0.0075 0.0006 0.0109 0.0007 0.0003 0.0001 0.0168 0.0003 0.0005 0.0001 0.0003 0.0002 0.0049 0.0003 0.0008 0.0001 0.0004 0.0002
Cell Periphery 0.0009 0.0003 0.0012 0.0003 0.0002 0 0.0012 0.0002 0.0001 0 0.0001 0 0.0006 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.6288 0.9225 0.7774 0.9307 0.8441 0.9067 0.3752 0.8672 0.8575 0.9457 0.8227 0.7798 0.7089 0.9096 0.9262 0.9822 0.8708 0.8868
Cytoplasmic Foci 0.054 0.0025 0.0082 0.0181 0.0081 0.0035 0.0724 0.0054 0.0065 0.0034 0.0426 0.0227 0.0294 0.0023 0.0102 0.002 0.0096 0.01
Eisosomes 0.0006 0 0.0009 0 0 0 0.0007 0 0.0001 0 0.0001 0 0.0007 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0159 0.0067 0.0087 0.0084 0.0039 0.0035 0.04 0.0025 0.0024 0.0026 0.0018 0.0054 0.0079 0.0013 0.0048 0.0008 0.0023 0.0039
Endosome 0.1091 0.0012 0.0098 0.0088 0.017 0.0014 0.1654 0.0005 0.0045 0.0013 0.0135 0.0225 0.026 0.0006 0.0069 0.0005 0.0084 0.006
Golgi 0.0468 0.0002 0.003 0.0008 0.0121 0.0001 0.0452 0.0005 0.003 0.0001 0.0277 0.0216 0.0158 0.0001 0.0008 0 0.0109 0.0033
Lipid Particles 0.0328 0.0002 0.0057 0.0002 0.0503 0.0001 0.0183 0.0002 0.0003 0 0.0137 0.0066 0.0088 0 0.0004 0 0.0035 0.0008
Mitochondria 0.0093 0.0001 0.0014 0.0003 0.0448 0.0001 0.0302 0.0001 0.0228 0.0001 0.0246 0.0033 0.018 0.0001 0.0003 0.0001 0.0092 0.0007
None 0.0068 0.06 0.1388 0.0192 0.0055 0.0737 0.1311 0.1095 0.0636 0.0418 0.0301 0.1169 0.0586 0.0834 0.0391 0.0108 0.055 0.0558
Nuclear Periphery 0.0032 0.0013 0.0088 0.0024 0.0016 0.0008 0.0266 0.0003 0.0009 0.0008 0.0006 0.0012 0.0184 0.0003 0.0009 0.0004 0.0005 0.0012
Nucleolus 0.0003 0 0.0007 0 0.0001 0 0.002 0 0 0 0.0001 0 0.0004 0 0.0001 0 0 0
Nucleus 0.0013 0.0019 0.0052 0.0046 0.0041 0.0081 0.0074 0.0007 0.0021 0.0024 0.0019 0.0027 0.0066 0.0009 0.003 0.0018 0.0014 0.0026
Peroxisomes 0.0052 0 0.001 0.0003 0.0029 0 0.0068 0.0013 0.0037 0.0001 0.0051 0.0066 0.0222 0 0.0007 0 0.0032 0.0034
Punctate Nuclear 0.0019 0.0004 0.0028 0.0013 0.001 0.0005 0.006 0.0002 0.0006 0.0003 0.0097 0.0018 0.0063 0.0001 0.0004 0.0002 0.0003 0.0007
Vacuole 0.0045 0.0007 0.0015 0.0014 0.0012 0.0004 0.0139 0.0002 0.0003 0.0003 0.0009 0.0006 0.0023 0.0002 0.0013 0.0002 0.0004 0.0004
Vacuole Periphery 0.0017 0.0001 0.0005 0.0002 0.0008 0.0001 0.0079 0 0.0001 0.0001 0.0004 0.0002 0.0011 0 0.0002 0 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.7453 24.1486 22.0545 26.6652 31.8812 29.0755 28.6425 33.3817 31.6517 29.7547
Translational Efficiency 0.9727 1.0064 1.0331 1.0798 0.6931 1.0286 1.0843 0.7948 1.0404 1.0143

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
354 1617 230 1617 1101 1632 296 647 1455 3249 526 2264

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 854.32 918.49 1296.51 1207.35 850.96 863.26 1223.14 1093.86 851.78 890.75 1255.22 1174.92
Standard Deviation 94.08 137.65 160.72 169.23 107.49 133.38 145.60 161.84 104.40 138.31 156.68 174.84
Intensity Change Log 2 — 0.104487 0.601785 0.498995 — 0.020704 0.523426 0.362265 — 0.063286 0.563215 0.432384

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000137 0.000692 0.000754 0.000618 0.000175 0.000195 0.000514 0.001012 0.000166 0.000443 0.000619 0.000731
Bud Neck 0.002999 0.003402 0.002120 0.002271 0.001605 0.004398 0.002000 0.003820 0.001944 0.003902 0.002053 0.002714
Bud Site 0.007093 0.013166 0.010981 0.019204 0.005218 0.013139 0.012412 0.018907 0.005674 0.013153 0.011786 0.019119
Cell Periphery 0.000124 0.000094 0.000278 0.000070 0.000108 0.000068 0.000132 0.000128 0.000112 0.000081 0.000196 0.000087
Cytoplasm 0.640193* 0.731915* 0.646676* 0.723848* 0.718319* 0.712313* 0.576708* 0.570401* 0.699311* 0.722069* 0.607302* 0.679996*
Cytoplasmic Foci 0.203603* 0.111520 0.047792 0.018734 0.129562 0.124035 0.030901 0.038765 0.147576 0.117806 0.038287 0.024458
Eisosomes 0.000070 0.000025 0.000037 0.000008 0.000036 0.000029 0.000029 0.000027 0.000044 0.000027 0.000033 0.000014
Endoplasmic Reticulum 0.001288 0.001300 0.003042 0.000463 0.001776 0.000529 0.001946 0.002281 0.001657 0.000913 0.002425 0.000982
Endosome 0.010988 0.008642 0.009332 0.002860 0.008614 0.009060 0.006478 0.020405 0.009192 0.008852 0.007726 0.007874
Golgi 0.000910 0.003495 0.000608 0.002365 0.000953 0.002667 0.000205 0.009682 0.000943 0.003079 0.000381 0.004456
Lipid Particles 0.001933 0.001903 0.002852 0.000779 0.002453 0.001387 0.001644 0.002720 0.002326 0.001644 0.002172 0.001334
Mitochondria 0.000151 0.002195 0.000131 0.000395 0.000507 0.001698 0.000436 0.003071 0.000421 0.001945 0.000302 0.001160
Mitotic Spindle 0.001832 0.005150 0.004814 0.041801 0.001801 0.004012 0.009664 0.037053 0.001808 0.004579 0.007543 0.040444
None 0.001165 0.002947 0.001414 0.005660 0.002650 0.002799 0.004995 0.003714 0.002289 0.002873 0.003429 0.005104
Nuclear Periphery 0.000129 0.000167 0.000749 0.000793 0.000317 0.000137 0.000868 0.001345 0.000271 0.000152 0.000816 0.000951
Nuclear Periphery Foci 0.001348 0.002132 0.022054 0.002344 0.003772 0.001274 0.006105 0.002920 0.003182 0.001701 0.013079 0.002509
Nucleolus 0.000215 0.000628 0.000177 0.000193 0.000469 0.000847 0.000153 0.000618 0.000408 0.000738 0.000163 0.000314
Nucleus 0.040079 0.050252 0.086510 0.118444 0.059382 0.044367 0.121383 0.050004 0.054685 0.047296 0.106134 0.098885
Peroxisomes 0.000866 0.002198 0.000271 0.000975 0.000756 0.001745 0.000460 0.003249 0.000782 0.001970 0.000377 0.001625
Vacuole 0.084375 0.056735 0.158967 0.057023 0.060786 0.073872 0.222500* 0.223506* 0.066525 0.065343 0.194720 0.104600
Vacuole Periphery 0.000502 0.001442 0.000443 0.001152 0.000743 0.001429 0.000464 0.006372 0.000684 0.001436 0.000455 0.002644

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.78 -8.84 -3.76 0.98 5.19 -0.60 -6.25 -2.55 -2.47 -0.77 -1.75 -10.75 -4.29 -0.15 2.31
Bud Neck -0.79 1.38 2.39 2.67 1.40 -6.10 -2.40 -3.28 3.12 -1.61 -5.89 -2.10 -2.11 4.69 -0.14
Bud Site -2.97 -2.24 -1.11 2.10 1.23 -4.62 -2.62 -3.35 0.89 -0.77 -5.88 -3.86 -3.29 2.35 0.40
Cell Periphery 3.27 -7.55 6.45 3.23 10.63 6.89 -2.19 2.39 -1.56 3.65 6.03 -7.46 6.84 0.98 11.00
Cytoplasm -6.54 3.31 1.78 13.49 -2.49 0.91 14.68 15.57 15.28 -1.79 -2.87 14.22 14.03 19.29 -5.45
Cytoplasmic Foci 9.84 17.05 21.48 26.08 7.36 0.94 23.30 23.06 23.66 -1.48 6.44 26.80 32.78 36.62 5.52
Eisosomes 9.09 4.98 11.92 8.87 8.89 3.04 1.02 4.11 1.32 2.88 8.24 2.93 14.16 9.25 10.83
Endoplasmic Reticulum -0.05 -2.25 1.14 5.49 7.00 5.82 -1.01 1.61 -2.07 2.40 3.11 -2.71 3.93 1.87 8.19
Endosome 1.79 1.43 8.28 10.44 9.09 -0.29 5.60 1.32 1.47 -1.95 0.67 4.19 9.56 9.45 5.40
Golgi -3.68 1.64 0.93 4.04 -0.16 -3.18 5.81 -1.69 0.04 -2.55 -4.85 5.23 -0.59 3.55 -2.49
Lipid Particles 0.01 -2.77 2.39 1.89 5.55 3.32 2.01 1.68 -0.50 0.50 2.51 0.27 3.48 1.12 4.61
Mitochondria -3.17 0.59 -4.07 2.91 -5.00 -2.25 0.99 -0.82 1.31 -1.59 -3.47 1.19 -0.25 3.54 -2.29
Mitotic Spindle -1.60 -0.92 -7.15 -6.54 -5.42 -1.66 -1.96 -4.94 -4.36 -3.48 -2.49 -2.07 -9.38 -8.14 -6.67
None -4.40 -0.48 -13.10 -6.36 -13.74 -0.14 -0.91 -0.90 -1.11 0.32 -1.04 -0.44 -5.12 -6.60 -4.60
Nuclear Periphery -1.84 -11.65 -9.38 -8.98 -0.91 1.92 -8.51 -3.15 -4.24 1.19 1.63 -10.20 -6.73 -9.63 1.03
Nuclear Periphery Foci -3.39 -12.12 -4.36 -0.57 11.56 3.59 -2.80 1.65 -4.63 6.29 2.75 -9.84 1.50 -3.88 12.23
Nucleolus -1.95 1.60 -0.85 1.86 -2.30 -0.79 2.07 -0.05 0.72 -1.63 -1.16 2.11 0.85 1.79 -2.26
Nucleus -3.91 -7.81 -26.34 -24.94 -6.17 4.70 -8.54 -2.70 -6.72 6.98 2.78 -10.46 -20.28 -25.99 -0.43
Peroxisomes -3.48 4.24 3.17 5.01 -0.69 -3.94 3.01 -0.66 1.36 -1.77 -4.97 4.01 1.19 5.64 -1.90
Vacuole 4.13 -8.99 -11.49 -19.70 3.33 -3.75 -16.41 -23.01 -21.77 -0.44 -0.23 -18.72 -26.07 -27.28 5.07
Vacuole Periphery -3.42 1.43 1.05 3.57 0.36 -1.55 3.84 -1.56 -0.45 -2.39 -2.81 3.91 -0.16 2.14 -1.94
Standard name
Human Ortholog
Description ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Utr1

Utr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Utr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available