ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.53 0.79 0.78 0.95 0.86 0.85 – 0.8 0.76 0.69 0.63 0.56 0.86 0.95 0.75 0.76 0.68 0.28 0.4 0.5 0.48 0.67 0.61
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0 0 – 0 0 0 0 0.1 0 0 0 0.05 0.07 0.05 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0.28 0.07 – 0.25 0.25 0.38 0.33 0 0 0 0 0 0 0.05 0 0.11 0.08 0.06 0.06
Nucleus 0.12 0.08 0.11 0.08 0 0 0.06 – 0 0 0 0.06 0.06 0 0 0 0 0 0.27 0.19 0.1 0.07 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.07 0.09
Vac/Vac Membrane 0.45 0.48 0.07 0.14 0 0 0 – 0 0 0 0 0.42 0.13 0.07 0.29 0.25 0.31 0.22 0.22 0.12 0.15 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 1 0 4 5 1 0 4 9
Bud 1 2 0 1 0 3 1 – 2 1 2 4 0 0 1 0 0 1 7 12 0 2 10 19
Bud Neck 0 0 2 0 2 3 5 – 5 5 4 5 0 0 0 0 0 0 1 3 1 2 4 8
Bud Site 0 0 0 0 0 0 0 – 0 0 4 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 0 0 0 1 – 0 2 0 1 0 0 0 2 4 0 0 0 0 0 1 2
Cytoplasm 105 239 102 67 232 152 246 – 199 219 188 193 175 338 482 188 168 120 86 214 89 72 353 319
Endoplasmic Reticulum 1 4 4 0 2 0 5 – 0 0 0 9 2 1 0 18 12 8 0 1 2 2 4 3
Endosome 22 14 0 0 0 0 2 – 0 3 0 2 30 14 12 8 10 13 14 16 3 5 18 30
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 0 1 0 2 0 1 4 0 2 2 3
Mitochondria 17 17 1 1 1 50 19 – 63 73 104 102 1 0 0 3 2 3 13 17 19 12 33 32
Nucleus 32 36 14 7 1 2 17 – 6 6 8 18 20 1 1 3 2 1 81 103 17 11 15 5
Nuclear Periphery 1 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 5 2 0 0 4 3
Nucleolus 0 0 0 0 0 1 1 – 0 0 0 0 0 0 0 0 1 0 2 1 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 – 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 3 1 2 0 7 – 1 4 6 5 3 0 0 1 1 0 13 18 10 9 36 47
Vac/Vac Membrane 117 216 9 12 8 1 7 – 8 9 5 6 131 53 35 72 56 54 67 118 21 22 23 24
Unique Cell Count 260 452 129 86 243 176 290 249 290 271 307 315 394 510 252 222 176 307 535 180 151 530 523
Labelled Cell Count 297 529 136 89 248 212 312 284 323 321 346 362 408 532 295 259 200 307 535 180 151 530 523


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 4.8 4.8 5.3 5.4 3.4 4.4 – 3.8 3.9 3.6 3.8 5.3 5.1 5.1 5.6 6.2 5.6 4.9 4.5 4.7
Std Deviation (1e-4) 1.5 1.0 0.6 2.1 1.7 1.2 1.5 – 1.4 1.0 0.6 0.7 1.2 1.5 1.3 1.1 1.8 1.5 1.9 1.6 1.8
Intensity Change (Log2) – – – 0.13 0.14 -0.53 -0.14 – -0.36 -0.31 -0.43 -0.36 0.13 0.08 0.08 0.22 0.37 0.22 0.0 -0.1 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.9 4.2 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2784 -0.8999 -1.1635 -1.3394 -1.4228 -1.0764 0.9931 0.8114 0.9437 1.0858 1.2913 0.9399 2.5908 3.1957 3.1919 3.4263 3.1745 3.5631
Actin 0.0135 0.0056 0.0052 0.0467 0.0065 0.0264 0.0446 0.0005 0.0109 0.0482 0.0095 0.0023 0.0003 0.0007 0.0004 0.0008 0.0005 0.0008
Bud 0.0004 0.0004 0.0002 0.0001 0.0001 0.0002 0.0104 0.0134 0.0045 0.014 0.0009 0.0048 0.0001 0.0002 0.0001 0.0002 0.0001 0.0006
Bud Neck 0.004 0.0003 0.0004 0.0004 0.0003 0.0012 0.0102 0.0012 0.0082 0.0021 0.0014 0.0042 0.0005 0.0005 0.0005 0.0006 0.0006 0.0023
Bud Periphery 0.0004 0.0004 0.0003 0.0001 0.0002 0.0003 0.0033 0.0014 0.0022 0.0021 0.0015 0.0018 0.0001 0.0001 0.0001 0.0003 0.0002 0.0004
Bud Site 0.0029 0.0036 0.0008 0.0004 0.0002 0.0003 0.0064 0.0181 0.0095 0.0345 0.0024 0.0011 0.0003 0.0015 0.0007 0.0003 0.0001 0.0001
Cell Periphery 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0006 0.0003 0.0003 0.002 0.0002 0.0003 0 0.0001 0.0001 0 0 0
Cytoplasm 0.2961 0.3578 0.3459 0.3218 0.3483 0.3611 0.2218 0.2859 0.2736 0.1721 0.337 0.2984 0.276 0.2926 0.2904 0.2531 0.2919 0.2918
Cytoplasmic Foci 0.0193 0.0188 0.017 0.0299 0.0181 0.0154 0.0365 0.009 0.0181 0.0337 0.0135 0.0161 0.0077 0.0069 0.0114 0.0063 0.0283 0.0074
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0
Endoplasmic Reticulum 0.0107 0.0109 0.012 0.0055 0.0071 0.005 0.0077 0.0059 0.0037 0.0017 0.0057 0.0029 0.004 0.0042 0.005 0.0024 0.0031 0.003
Endosome 0.0228 0.0171 0.0141 0.0099 0.0292 0.0165 0.0275 0.0085 0.0077 0.0071 0.0103 0.0102 0.004 0.0035 0.0066 0.003 0.0061 0.0049
Golgi 0.0022 0.0041 0.0013 0.0004 0.003 0.0014 0.0083 0.0002 0.0031 0.0068 0.0036 0.0015 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001
Lipid Particles 0.0031 0.0043 0.0095 0.0005 0.0034 0.0012 0.0179 0.0003 0.0091 0.0044 0.0088 0.0044 0.0006 0.0004 0.0011 0.0002 0.0006 0.0003
Mitochondria 0.0012 0.0015 0.0012 0.0003 0.0017 0.0021 0.0033 0.0013 0.0149 0.0017 0.0214 0.0016 0.0004 0.0005 0.0007 0.0008 0.0007 0.0006
None 0.4698 0.4402 0.4412 0.4798 0.5362 0.4638 0.4509 0.4593 0.4222 0.517 0.429 0.3381 0.5836 0.5503 0.5237 0.6222 0.5384 0.503
Nuclear Periphery 0.0289 0.0185 0.0237 0.0076 0.0186 0.0068 0.0137 0.0122 0.0109 0.004 0.0092 0.0103 0.01 0.0065 0.0131 0.0046 0.0138 0.0076
Nucleolus 0.0016 0.0012 0.0013 0.0009 0.0004 0.0006 0.0061 0.0018 0.003 0.0044 0.0015 0.0033 0.0012 0.0016 0.0013 0.0011 0.001 0.0018
Nucleus 0.0817 0.1001 0.1073 0.0763 0.0097 0.0821 0.0705 0.1621 0.1502 0.1059 0.1149 0.2454 0.0904 0.1172 0.1248 0.0743 0.0911 0.1431
Peroxisomes 0.0011 0.0017 0.0014 0.0007 0.0006 0.0032 0.0188 0.0003 0.0096 0.0072 0.0085 0.0019 0.0001 0.0005 0.0006 0.0003 0.0008 0.0002
Punctate Nuclear 0.0318 0.0068 0.011 0.0151 0.0129 0.0104 0.0338 0.015 0.0348 0.0276 0.0174 0.0483 0.0187 0.0099 0.0164 0.0278 0.0211 0.03
Vacuole 0.0068 0.0055 0.0048 0.0031 0.0022 0.0014 0.0056 0.0028 0.0027 0.003 0.0024 0.0024 0.0017 0.0024 0.0021 0.0012 0.001 0.0015
Vacuole Periphery 0.0013 0.0008 0.0011 0.0003 0.0012 0.0003 0.0014 0.0005 0.0006 0.0003 0.0008 0.0007 0.0003 0.0003 0.0006 0.0002 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.4152 12.4858 14.4122 14.0122 14.0848 10.8573 12.2737 12.3075 13.5901 15.2638
Translational Efficiency 2.3917 1.9349 1.3763 1.7814 1.5513 1.959 1.6721 1.4748 1.7723 1.2397

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1639 984 1513 1697 1993 2763 2687 175 3632 3747 4200 1872

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 656.94 681.86 875.25 792.91 652.98 709.05 858.64 868.90 654.77 701.91 864.62 800.01
Standard Deviation 70.86 102.73 113.37 113.87 70.87 87.72 117.12 93.83 70.90 92.67 116.06 114.31
Intensity Change Log 2 — 0.053714 0.413934 0.271396 — 0.118849 0.395015 0.412151 — 0.086550 0.404534 0.343277

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000062 0.000419 0.000354 0.000614 0.000056 0.000067 0.000200 0.000153 0.000059 0.000159 0.000256 0.000571
Bud Neck 0.006692 0.012876 0.005306 0.004971 0.007319 0.011415 0.009157 0.000683 0.007036 0.011799 0.007770 0.004570
Bud Site 0.001351 0.005868 0.002468 0.007097 0.001044 0.005551 0.001387 0.000312 0.001183 0.005634 0.001776 0.006463
Cell Periphery 0.000113 0.000237 0.000133 0.000121 0.000074 0.000171 0.000095 0.000156 0.000091 0.000189 0.000109 0.000124
Cytoplasm 0.352144* 0.290298* 0.048457 0.111395* 0.382676* 0.346870* 0.084247 0.086651 0.368898* 0.332014* 0.071354 0.109082*
Cytoplasmic Foci 0.107703 0.086056 0.001771 0.002505 0.084598 0.036916 0.000919 0.016026 0.095025 0.049820 0.001226 0.003769
Eisosomes 0.000075 0.000072 0.000021 0.000021 0.000053 0.000039 0.000012 0.000052 0.000063 0.000047 0.000015 0.000024
Endoplasmic Reticulum 0.000889 0.005214 0.004958 0.002920 0.000760 0.000659 0.003357 0.002202 0.000818 0.001856 0.003934 0.002853
Endosome 0.002957 0.006160 0.000993 0.000825 0.001919 0.002929 0.000660 0.000989 0.002387 0.003777 0.000780 0.000840
Golgi 0.000946 0.001630 0.000170 0.000059 0.000502 0.000431 0.000065 0.000050 0.000702 0.000746 0.000103 0.000058
Lipid Particles 0.010088 0.010586 0.000522 0.000582 0.005232 0.003948 0.000227 0.002063 0.007424 0.005691 0.000333 0.000720
Mitochondria 0.007048 0.006975 0.000981 0.001337 0.002204 0.003996 0.001101 0.000315 0.004390 0.004778 0.001058 0.001241
Mitotic Spindle 0.001699 0.008819 0.001503 0.010055 0.000957 0.005080 0.008733 0.000034 0.001292 0.006061 0.006128 0.009118
None 0.006775 0.026915 0.004964 0.009108 0.004663 0.011206 0.006795 0.005298 0.005616 0.015331 0.006135 0.008752
Nuclear Periphery 0.001817 0.003904 0.001761 0.002138 0.001429 0.001935 0.002556 0.001005 0.001604 0.002452 0.002270 0.002032
Nuclear Periphery Foci 0.000575 0.004749 0.000711 0.000525 0.000454 0.000373 0.000340 0.000486 0.000509 0.001522 0.000474 0.000521
Nucleolus 0.002142 0.013126 0.002018 0.001604 0.001994 0.003967 0.001951 0.001940 0.002061 0.006372 0.001975 0.001636
Nucleus 0.480618* 0.488807* 0.916212* 0.835504* 0.488476* 0.550639* 0.874249* 0.871281* 0.484930* 0.534401* 0.889366* 0.838849*
Peroxisomes 0.005262 0.006647 0.000135 0.000277 0.002978 0.002821 0.000068 0.000311 0.004008 0.003825 0.000092 0.000280
Vacuole 0.010556 0.019385 0.006300 0.008201 0.012425 0.010589 0.003613 0.009892 0.011582 0.012899 0.004581 0.008359
Vacuole Periphery 0.000489 0.001258 0.000261 0.000141 0.000186 0.000399 0.000269 0.000101 0.000323 0.000625 0.000266 0.000137

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.47 -7.05 -2.92 -0.70 -0.66 -0.97 -12.69 -2.65 -2.51 -0.20 -6.69 -12.16 -3.07 -2.37 -1.34
Bud Neck -3.25 -0.19 -0.12 3.35 0.09 -3.28 -4.12 6.57 10.42 14.50 -4.70 -3.88 0.88 5.68 5.24
Bud Site -4.49 -2.42 -3.92 -0.45 -2.37 -5.46 -2.98 0.89 5.62 3.14 -6.66 -3.51 -4.07 -0.26 -3.09
Cell Periphery -6.67 -1.39 -0.66 6.14 0.88 -7.90 -3.33 -4.02 0.15 -3.16 -9.21 -2.40 -3.75 5.04 -1.71
Cytoplasm 6.07 44.02 33.08 19.39 -12.28 4.47 46.51 25.15 22.77 -0.69 5.98 62.84 46.63 39.25 -8.71
Cytoplasmic Foci 3.43 26.15 26.11 17.44 -0.78 13.59 27.33 14.53 5.42 -4.01 14.68 37.75 36.28 25.09 -4.97
Eisosomes -0.10 12.00 12.61 13.79 0.46 4.82 14.93 -0.74 -3.22 -7.46 5.49 19.33 13.59 9.69 -5.88
Endoplasmic Reticulum -9.67 -17.21 -12.68 2.14 5.46 1.21 -18.55 -5.10 -5.34 2.48 -7.68 -25.13 -14.02 -8.53 3.56
Endosome -5.11 4.35 6.12 9.58 1.26 -2.72 4.99 3.17 4.78 -1.20 -4.16 6.75 7.31 9.43 -0.17
Golgi -1.77 4.68 5.97 4.51 1.06 0.31 2.19 2.23 3.08 0.35 -0.20 4.59 4.98 5.31 0.97
Lipid Particles -0.47 12.38 12.24 9.70 -1.05 2.05 11.85 6.23 4.20 -6.51 2.81 16.90 15.63 12.51 -6.09
Mitochondria 0.10 5.00 4.61 4.51 -2.03 -2.70 1.78 3.91 7.30 11.39 -0.54 5.22 4.85 6.69 -0.78
Mitotic Spindle -3.34 -0.36 -5.30 -1.63 -5.24 -4.31 -6.78 2.55 5.41 7.46 -5.22 -6.51 -5.56 -2.80 -1.81
None -6.38 2.43 -1.61 5.68 -3.95 -6.52 -2.37 -0.56 4.07 0.84 -8.56 -0.16 -2.96 5.06 -2.83
Nuclear Periphery -3.72 -1.45 -5.46 1.66 -4.13 -2.39 -8.04 1.01 4.11 11.66 -4.10 -8.00 -6.43 -1.34 0.10
Nuclear Periphery Foci -4.54 -1.03 -1.93 3.81 -0.73 0.39 -1.28 -1.48 -1.62 -0.44 -3.95 -1.51 -2.42 1.28 -1.42
Nucleolus -8.32 -0.54 0.60 8.36 0.94 -2.87 -0.61 0.50 3.50 1.29 -7.69 -0.85 0.73 7.89 1.51
Nucleus -0.57 -50.79 -37.68 -28.51 11.60 -7.17 -51.46 -23.79 -20.06 0.56 -6.98 -71.27 -48.40 -40.06 8.82
Peroxisomes -1.24 7.52 7.26 7.51 -1.00 0.16 5.18 4.75 6.83 -5.09 0.29 8.99 8.36 9.72 -1.78
Vacuole -6.21 0.43 -2.84 3.04 -2.66 1.78 8.18 -1.13 -1.47 -2.40 -1.83 6.39 -2.40 -1.14 -5.69
Vacuole Periphery -3.83 1.76 3.03 6.69 1.72 -4.47 -0.55 4.59 6.07 1.38 -4.01 0.94 3.38 8.36 1.56
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages nucleus (35%), cytoplasm (6%), mixed (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YJR056C

YJR056C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yjr056c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available