Standard name
Human Ortholog
Description Member of the Puf family of RNA-binding proteins; interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl; JSN1 has a paralog, PUF2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.19 0.15 0.19 0.2 0.31 0.15 0.09 0.06 0 0 0.09 0 0.18 0.3 0.39 0.07 0 0.07 0.19 0.21 0.17 0.15 0.12 0.12
Bud 0.06 0.07 0.09 0.11 0.16 0.08 0.06 0.08 0.08 0.05 0.06 0.07 0.07 0.1 0.15 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.12 0.09 0.08 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.44 0.42 0.29 0.36 0.28 0.34 0.21 0.15 0.18 0.19 0.17 0.18 0.65 0.57 0.56 0.64 0.62 0.6 0.28 0.19 0.22 0.14 0.15 0.33
Cytoplasm 0.54 0.51 0.6 0.46 0.36 0.48 0.54 0.55 0.55 0.57 0.36 0.57 0.53 0.4 0.32 0.44 0.46 0.51 0.22 0.24 0.2 0.47 0.45 0.34
Endoplasmic Reticulum 0.08 0.1 0.09 0 0.11 0.12 0.08 0.06 0.05 0 0.15 0 0 0.05 0.06 0 0 0 0.15 0.09 0.16 0.08 0.1 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.13 0.15 0.27 0.3 0.36 0.45 0.47 0.46 0.43 0 0 0.1 0 0 0 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.06 0.09 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 23 44 34 68 40 30 27 12 11 20 11 31 85 122 13 4 8 16 42 49 18 31 33
Bud 3 10 22 19 34 20 21 33 23 16 14 24 13 28 46 6 2 2 2 8 4 1 5 2
Bud Neck 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 25 34 10 23 16
Bud Site 0 0 1 3 1 3 3 3 3 1 0 2 0 3 4 0 0 0 – – – – – –
Cell Periphery 21 63 67 61 62 88 72 66 50 65 41 64 114 159 174 117 56 73 23 37 60 17 40 93
Cytoplasm 26 76 140 77 78 125 183 239 152 192 85 205 92 113 100 81 42 62 18 48 55 57 122 97
Endoplasmic Reticulum 4 15 21 6 24 32 27 25 13 9 36 7 3 13 18 4 3 4 12 18 44 9 28 28
Endosome 0 0 0 0 3 0 4 5 0 1 0 1 0 2 0 1 0 1 0 2 1 0 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 0 3 3
Mitochondria 1 5 6 22 32 72 102 156 124 159 109 155 0 3 30 6 1 3 1 6 16 0 4 2
Nucleus 0 0 1 0 0 1 3 2 1 1 0 1 1 0 2 7 3 4 0 0 0 0 0 0
Nuclear Periphery 0 1 1 0 1 0 2 0 0 0 1 2 0 0 1 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 4 1 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 1 0 3 2 0 1 0 0 0 1 2 2 0 4 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 3 2 9 10 2 8 2 26 0 1 1 11 8 8 0 6 8 4 7 1
Unique Cell Count 48 149 235 168 218 262 341 437 275 339 235 362 175 280 313 184 91 122 84 200 282 123 275 284
Labelled Cell Count 64 195 304 222 312 384 460 569 381 465 308 499 254 408 500 248 120 170 84 200 282 123 275 284


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 7.1 5.8 5.7 6.1 5.4 5.0 5.0 4.5 4.4 4.3 4.2 8.2 8.2 7.3 11.9 13.4 13.1 7.7 8.6 9.4
Std Deviation (1e-4) 1.4 1.1 1.1 1.0 1.2 0.9 0.9 1.0 1.1 0.9 0.8 1.1 1.1 1.3 1.4 2.4 2.6 2.7 1.3 1.6 1.6
Intensity Change (Log2) – – – -0.02 0.09 -0.09 -0.19 -0.19 -0.36 -0.4 -0.42 -0.45 0.51 0.5 0.34 1.05 1.22 1.19 0.42 0.58 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.4 3.1 -1.0 -3.5 -5.0 0 0 0 0 -0.3 3.0 5.1 -3.5 -3.3 -3.1
Bud 0.6 2.0 -0.7 -1.4 -0.8 0 0 0 0 -0.7 0.2 1.9 -2.5 -2.2 -2.8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.7 0 1.2 -2.0 -4.2 0 0 0 0 7.4 6.4 6.3 7.2 5.5 5.7
Cytoplasm -2.7 -5.1 -2.6 -1.4 -1.2 -1.0 -0.7 -5.1 -0.7 -1.4 -4.3 -6.5 -3.2 -2.2 -1.6
Endoplasmic Reticulum -2.1 0.7 1.2 -0.4 -1.6 0 0 0 0 -3.1 -2.0 -1.4 -2.9 -1.7 -2.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.1 4.7 7.6 8.3 9.6 0 0 0 0 0 0 3.3 0.4 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 4.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8177 5.5218 5.0703 4.7758 4.1385 5.1564 6.0951 6.0795 6.269 5.7542 5.103 5.7241 7.5662 6.8819 6.8866 6.7652 7.1567 7.5059
Actin 0.1732 0.0347 0.0872 0.1262 0.1015 0.0827 0.0352 0.0391 0.0539 0.0302 0.0222 0.1242 0.0775 0.0082 0.1364 0.1459 0.1066 0.0407
Bud 0.0032 0.0018 0.0049 0.0057 0.0032 0.002 0.0012 0.0015 0.0024 0.0047 0.0013 0.0027 0.0006 0.0011 0.004 0.0046 0.0014 0.0029
Bud Neck 0.0191 0.0079 0.0329 0.0302 0.0311 0.136 0.0345 0.0158 0.0307 0.0674 0.1 0.0966 0.0072 0.014 0.0231 0.0441 0.0338 0.0812
Bud Periphery 0.0088 0.0064 0.0347 0.0509 0.0263 0.0214 0.0047 0.0043 0.0201 0.0456 0.0086 0.0275 0.0034 0.0026 0.0281 0.07 0.0098 0.0263
Bud Site 0.0285 0.1813 0.0818 0.0105 0.0039 0.0031 0.0675 0.2085 0.069 0.0087 0.0345 0.0062 0.0439 0.258 0.0499 0.0058 0.0032 0.0064
Cell Periphery 0.1734 0.1599 0.1815 0.249 0.1855 0.1776 0.2993 0.1761 0.2347 0.1645 0.1453 0.2505 0.3418 0.1107 0.2373 0.275 0.1873 0.2761
Cytoplasm 0.2335 0.5811 0.4735 0.4306 0.5682 0.5325 0.4382 0.5293 0.5241 0.4869 0.5776 0.4214 0.4287 0.6005 0.4585 0.4075 0.5335 0.4933
Cytoplasmic Foci 0.0404 0.0019 0.008 0.0043 0.0111 0.0022 0.0349 0.0054 0.004 0.012 0.0129 0.0037 0.0061 0.0005 0.0074 0.0045 0.0007 0.0043
Eisosomes 0.0046 0.0028 0.0017 0.0018 0.0023 0.0037 0.0047 0.0024 0.0028 0.0009 0.0003 0.0045 0.0282 0.0002 0.0021 0.0051 0.0088 0.0012
Endoplasmic Reticulum 0.0652 0.0074 0.02 0.032 0.0103 0.0187 0.0308 0.006 0.015 0.017 0.0045 0.0079 0.0201 0.0018 0.0087 0.0064 0.0061 0.0184
Endosome 0.081 0.0015 0.0092 0.0111 0.0066 0.0019 0.0088 0.0006 0.0101 0.0133 0.0383 0.0096 0.0077 0.0001 0.0039 0.0019 0.0001 0.0048
Golgi 0.0592 0.0041 0.0151 0.0189 0.0127 0.0037 0.0045 0.0023 0.0039 0.026 0.0477 0.019 0.0084 0.0003 0.0123 0.0206 0.0006 0.0076
Lipid Particles 0.0403 0.0011 0.0086 0.0057 0.0199 0.0029 0.0044 0.0013 0.0021 0.0035 0.0012 0.0012 0.017 0.0002 0.0111 0.0036 0.0004 0.0017
Mitochondria 0.0149 0.0002 0.0257 0.0133 0.0027 0.0056 0.001 0.0015 0.0004 0.064 0.0011 0.0045 0.0009 0 0.0058 0.0005 0 0.0116
None 0.0049 0.0046 0.0044 0.0008 0.0013 0.0011 0.0183 0.0029 0.0224 0.0439 0.0009 0.0179 0.0029 0.0002 0.0013 0.0009 0.1064 0.0011
Nuclear Periphery 0.0128 0.0007 0.0014 0.0019 0.0004 0.0012 0.0019 0.0004 0.001 0.001 0.0002 0.0003 0.0012 0.0002 0.0007 0.0004 0.0004 0.0006
Nucleolus 0.001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0.0001
Nucleus 0.0034 0.0006 0.0008 0.0008 0.0004 0.0006 0.0011 0.0004 0.0005 0.0007 0.0004 0.0003 0.0003 0.0005 0.0003 0.0003 0.0002 0.0007
Peroxisomes 0.0153 0.0001 0.0042 0.003 0.0093 0.0002 0.0032 0.001 0.0003 0.0054 0.0004 0.0005 0.0013 0 0.0069 0.0013 0 0.0177
Punctate Nuclear 0.0038 0.0002 0.0006 0.0004 0.0003 0.0001 0.0035 0.0003 0.0003 0.0002 0.0001 0.0001 0.0007 0 0.0006 0.0002 0.0001 0.0001
Vacuole 0.0106 0.0016 0.0032 0.0024 0.0024 0.002 0.0017 0.0012 0.002 0.0026 0.0024 0.001 0.0018 0.0008 0.0012 0.0014 0.0006 0.0028
Vacuole Periphery 0.0029 0.0001 0.0004 0.0005 0.0002 0.0007 0.0002 0 0.0002 0.0016 0.0002 0.0002 0.0002 0 0.0002 0.0001 0 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.0718 21.6786 29.045 40.8205 24.0182 25.5837 35.6948 41.2999 39.5234 38.4637
Translational Efficiency 0.81 0.9132 0.8098 0.5067 0.6558 0.9616 0.6252 0.5998 0.7003 0.6377

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Member of the Puf family of RNA-binding proteins; interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl; JSN1 has a paralog, PUF2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (31%), ER (7%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Jsn1

Jsn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Jsn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available