ORF | |
---|---|
Human Ortholog | |
Description | Xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 1 |
Bud | 0 | 4 | 0 | 2 | 0 | 3 | 5 | 6 | 7 | 9 | 7 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 7 | 8 | 11 |
Bud Neck | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 2 | 1 | 8 | 6 | 4 | 5 | 4 | 5 | 8 | 0 | 1 | 0 | 3 | 7 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 165 | 128 | 368 | 310 | 340 | 488 | 488 | 506 | 375 | 294 | 290 | 281 | 107 | 373 | 397 | 222 | 243 | 211 | 157 | 126 | 318 | 205 | 228 | 261 |
Endoplasmic Reticulum | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 2 | 0 | 0 | 1 | 10 | 9 | 15 | 0 | 0 | 1 | 3 | 0 | 2 |
Endosome | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 2 | 3 | 1 | 1 | 3 |
Golgi | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 1 | 0 | 1 |
Mitochondria | 9 | 15 | 1 | 12 | 12 | 55 | 82 | 38 | 143 | 126 | 108 | 116 | 2 | 1 | 0 | 4 | 7 | 4 | 8 | 2 | 17 | 1 | 2 | 5 |
Nucleus | 0 | 0 | 2 | 0 | 5 | 4 | 11 | 19 | 22 | 17 | 16 | 17 | 2 | 3 | 5 | 1 | 2 | 0 | 0 | 0 | 1 | 22 | 30 | 21 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 8 | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 1 | 0 | 4 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 24 | 22 | 22 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 2 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 0 | 4 | 5 | 5 | 6 |
Vac/Vac Membrane | 0 | 0 | 3 | 2 | 12 | 39 | 115 | 177 | 109 | 91 | 91 | 109 | 0 | 3 | 1 | 2 | 4 | 7 | 3 | 2 | 2 | 3 | 4 | 4 |
Unique Cell Count | 175 | 133 | 374 | 315 | 364 | 558 | 634 | 704 | 571 | 463 | 455 | 459 | 113 | 377 | 407 | 224 | 248 | 218 | 186 | 138 | 370 | 287 | 318 | 358 |
Labelled Cell Count | 179 | 147 | 380 | 330 | 373 | 606 | 712 | 753 | 669 | 551 | 527 | 551 | 115 | 381 | 408 | 243 | 273 | 242 | 186 | 138 | 370 | 287 | 318 | 358 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 3.7 | 5.5 | 5.1 | 5.7 | 5.5 | 5.8 | 6.9 | 5.9 | 6.4 | 8.0 | 8.3 | 4.9 | 5.6 | 5.6 | 5.8 | 6.6 | 7.2 | 7.4 | 7.8 | 7.5 |
Std Deviation (1e-4) | 0.7 | 3.1 | 1.7 | 1.9 | 1.8 | 1.6 | 1.9 | 2.1 | 2.0 | 2.5 | 13.4 | 11.0 | 1.6 | 1.7 | 1.6 | 1.7 | 1.4 | 1.7 | 4.2 | 4.5 | 4.8 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.06 | 0.0 | 0.07 | 0.33 | 0.11 | 0.23 | 0.55 | 0.59 | -0.16 | 0.02 | 0.02 | 0.08 | 0.26 | 0.39 | 0.43 | 0.5 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.7684 | 5.7559 | 4.7061 | 4.1099 | 4.3216 | 4.6885 | 4.1707 | 4.7823 | 4.2851 | 4.468 | 4.5341 | 4.2919 | 1.0624 | 1.3047 | 1.2823 | 1.72 | 0.9243 | 1.1148 |
Actin | 0.0394 | 0.0131 | 0.0162 | 0.0371 | 0.0109 | 0.0136 | 0.1088 | 0.0068 | 0.0462 | 0.0386 | 0.0007 | 0.0132 | 0.0033 | 0.0002 | 0.0005 | 0.0006 | 0.0003 | 0.0017 |
Bud | 0.0061 | 0.0025 | 0.0066 | 0.0017 | 0.0031 | 0.0042 | 0.0008 | 0.0177 | 0.0109 | 0.0006 | 0.0001 | 0.0019 | 0.0005 | 0.0001 | 0.0024 | 0.0002 | 0 | 0.0008 |
Bud Neck | 0.0105 | 0.0003 | 0.0027 | 0.0002 | 0.0038 | 0.0032 | 0.0009 | 0.0003 | 0.0008 | 0.0003 | 0.0001 | 0.0005 | 0.0062 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0056 |
Bud Periphery | 0.0056 | 0.0049 | 0.0056 | 0.005 | 0.004 | 0.0032 | 0.0012 | 0.0032 | 0.0077 | 0.0004 | 0.0001 | 0.003 | 0.0009 | 0 | 0.0019 | 0.0001 | 0 | 0.001 |
Bud Site | 0.0185 | 0.0029 | 0.0051 | 0.0015 | 0.0054 | 0.0014 | 0.0094 | 0.0179 | 0.0329 | 0.0022 | 0.0001 | 0.0004 | 0.0028 | 0.0004 | 0.0007 | 0.0001 | 0.0001 | 0.0039 |
Cell Periphery | 0.0009 | 0.0006 | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0008 | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0046 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.3494 | 0.5954 | 0.5913 | 0.5069 | 0.4537 | 0.5712 | 0.301 | 0.5011 | 0.4739 | 0.4976 | 0.4213 | 0.605 | 0.3605 | 0.5634 | 0.5508 | 0.5543 | 0.3936 | 0.652 |
Cytoplasmic Foci | 0.0234 | 0.012 | 0.0085 | 0.0089 | 0.0143 | 0.013 | 0.0258 | 0.0045 | 0.0146 | 0.0045 | 0.0072 | 0.0065 | 0.0191 | 0.0027 | 0.004 | 0.0049 | 0.017 | 0.0242 |
Eisosomes | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0002 | 0.0002 | 0 | 0 | 0.0009 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0051 | 0.0031 | 0.0023 | 0.001 | 0.0055 | 0.0026 | 0.0079 | 0.0018 | 0.0035 | 0.0022 | 0.0038 | 0.0012 | 0.0154 | 0.0018 | 0.0029 | 0.001 | 0.0007 | 0.0011 |
Endosome | 0.0185 | 0.0025 | 0.005 | 0.0019 | 0.009 | 0.0046 | 0.0425 | 0.0105 | 0.0038 | 0.004 | 0.0047 | 0.0006 | 0.0054 | 0.0004 | 0.0012 | 0.0015 | 0.0333 | 0.0025 |
Golgi | 0.0096 | 0.0051 | 0.0036 | 0.0033 | 0.0021 | 0.0023 | 0.026 | 0.0125 | 0.0042 | 0.0072 | 0.0002 | 0.0002 | 0.0019 | 0.0001 | 0.0001 | 0.0001 | 0.0073 | 0.001 |
Lipid Particles | 0.0328 | 0.0007 | 0.0062 | 0.0074 | 0.0016 | 0.0018 | 0.031 | 0.0004 | 0.0016 | 0.001 | 0.0001 | 0.0001 | 0.0208 | 0.0001 | 0.0002 | 0.0001 | 0.0127 | 0.0005 |
Mitochondria | 0.0359 | 0.0011 | 0.0018 | 0.0078 | 0.0038 | 0.001 | 0.01 | 0.0011 | 0.0021 | 0.0024 | 0.0003 | 0.0001 | 0.003 | 0.0001 | 0.001 | 0.0001 | 0.0003 | 0.0002 |
None | 0.392 | 0.3415 | 0.3317 | 0.3676 | 0.4338 | 0.358 | 0.3761 | 0.4175 | 0.386 | 0.4338 | 0.5497 | 0.3654 | 0.5342 | 0.4244 | 0.4173 | 0.4331 | 0.5299 | 0.2999 |
Nuclear Periphery | 0.0062 | 0.0011 | 0.0009 | 0.0004 | 0.0218 | 0.0014 | 0.0213 | 0.0005 | 0.0011 | 0.0009 | 0.0026 | 0.0002 | 0.0038 | 0.0004 | 0.004 | 0.0005 | 0.0003 | 0.0003 |
Nucleolus | 0.0014 | 0.0001 | 0.0002 | 0.0005 | 0.0014 | 0.0004 | 0.0016 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.001 | 0 | 0 | 0.0001 |
Nucleus | 0.0036 | 0.0017 | 0.0027 | 0.0016 | 0.0086 | 0.0089 | 0.0031 | 0.0012 | 0.0016 | 0.0015 | 0.0032 | 0.0008 | 0.0037 | 0.005 | 0.0077 | 0.0021 | 0.0005 | 0.0011 |
Peroxisomes | 0.0234 | 0.0091 | 0.0064 | 0.0455 | 0.0008 | 0.0018 | 0.0156 | 0.0002 | 0.0051 | 0.0004 | 0.0002 | 0.0003 | 0.0032 | 0 | 0.0001 | 0.0001 | 0.0022 | 0.0021 |
Punctate Nuclear | 0.0119 | 0.0005 | 0.0018 | 0.0006 | 0.0115 | 0.0061 | 0.0104 | 0.0005 | 0.0019 | 0.0015 | 0.0048 | 0.0003 | 0.0056 | 0.0003 | 0.0006 | 0.0004 | 0.0005 | 0.0016 |
Vacuole | 0.0042 | 0.0016 | 0.0008 | 0.0006 | 0.0023 | 0.0007 | 0.0028 | 0.0008 | 0.001 | 0.0003 | 0.0005 | 0.0002 | 0.0034 | 0.0004 | 0.0015 | 0.0005 | 0.0006 | 0.0003 |
Vacuole Periphery | 0.0011 | 0.0002 | 0.0003 | 0.0002 | 0.0017 | 0.0002 | 0.0026 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0007 | 0 | 0.0016 | 0.0001 | 0.0002 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.892 | 12.7799 | 16.3801 | 17.7479 | 13.4804 | 31.1744 | 21.732 | 22.9925 | 27.1078 | 23.2853 |
Translational Efficiency | 0.9682 | 1.3497 | 0.9638 | 1.342 | 1.4476 | 1.2414 | 0.9288 | 1.0729 | 1.0999 | 1.1161 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|