Standard name
Human Ortholog
Description Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 1.0 0.99 1.0 0.93 0.88 0.83 0.79 0.69 0.76 0.69 0.74 0.99 0.98 0.96 0.78 0.86 0.88 0.9 0.93 0.91 0.93 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0.06 0.17 0.07 0.26 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.08 0.09 0.12 0.12 0.17 0.14 0.1 0.12 0 0 0 0.23 0.16 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 1 0 1 1 1 0 0 0 0 1 0 1 1 1 0 0 0 0
Bud 3 0 0 0 0 0 1 3 1 1 5 0 0 0 0 1 0 0 0 1 2 1 2 3
Bud Neck 1 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 3 2 2 1 5 2 6 10 8 7 0 2 2 9 8 6 0 0 0 0 0 0
Cytoplasm 166 218 184 261 356 356 457 461 236 345 343 347 276 396 426 235 258 340 168 211 168 62 193 240
Endoplasmic Reticulum 2 1 1 0 1 3 7 14 3 10 7 8 0 4 3 6 5 9 0 0 2 0 0 1
Endosome 0 0 0 0 0 0 2 1 1 0 1 2 0 4 2 10 6 6 1 0 0 0 1 2
Golgi 1 1 0 0 0 0 0 0 0 0 1 0 0 1 0 3 6 6 1 0 0 0 0 0
Mitochondria 5 2 0 0 0 9 37 33 57 32 126 73 0 0 0 4 0 5 3 4 3 0 0 0
Nucleus 1 0 1 3 3 11 10 20 20 26 27 18 1 5 0 2 3 1 0 1 0 1 0 3
Nuclear Periphery 0 0 0 1 0 0 1 0 0 0 2 0 0 0 1 1 4 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 2 6 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 1 0 0 0 1 1 4 1 3 1 2 0 0 0 1
Vac/Vac Membrane 0 0 1 2 31 38 66 70 58 64 51 57 5 2 15 68 48 53 4 0 4 0 5 4
Unique Cell Count 174 219 185 262 381 406 548 581 340 453 494 467 279 406 443 302 300 385 187 229 185 67 209 260
Labelled Cell Count 180 222 190 269 393 418 587 606 384 490 572 512 282 415 450 352 342 436 187 229 185 67 209 260


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.5 6.7 6.5 8.0 7.0 7.2 7.1 6.5 6.9 6.3 6.5 6.7 7.0 6.7 12.5 13.3 12.4 6.3 6.6 7.1
Std Deviation (1e-4) 0.7 1.0 1.0 1.4 1.6 1.6 2.0 1.7 1.5 1.6 1.7 1.6 1.6 1.7 1.8 3.5 3.3 3.3 1.8 1.6 2.1
Intensity Change (Log2) – – – -0.05 0.26 0.07 0.11 0.09 -0.03 0.05 -0.09 -0.03 0.01 0.06 0.01 0.9 0.99 0.89 -0.1 -0.01 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 -3.2 -4.7 -5.7 -6.5 -8.2 -7.1 -8.4 -7.4 -0.6 -1.6 -2.3 -6.7 -5.1 -4.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.6 3.3 5.9 3.7 7.6 5.7 0 0 0 0 0 0
Nucleus 0 0 0 0 2.1 3.0 3.0 2.9 2.3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.7 4.0 4.7 4.7 5.7 5.1 4.3 4.7 0 0 0 6.7 5.5 5.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6132 1.155 0.8385 1.2351 1.0602 0.6845 0.423 0.6419 0.4942 0.5339 0.1549 0.562 -0.41 0.1981 0.1717 0.1635 -0.0432 0.0437
Actin 0.0164 0.0004 0.0014 0.0036 0.0265 0.0002 0.0596 0.0003 0.0195 0.0027 0.0003 0.0034 0.0086 0.001 0.0006 0.007 0.0006 0.0026
Bud 0.0024 0.0025 0.0008 0.001 0.0015 0.0001 0.0024 0.0001 0.0004 0.0004 0 0.0005 0.0039 0.0034 0.0016 0.0011 0.0008 0.0009
Bud Neck 0.0053 0.0004 0.0003 0.0009 0.0079 0.0005 0.003 0.0001 0.0008 0.0007 0.0001 0.0039 0.0003 0.0004 0.0002 0.0013 0.001 0.0041
Bud Periphery 0.0058 0.0011 0.0012 0.001 0.002 0.0001 0.007 0.0001 0.0004 0.0009 0 0.0007 0.0014 0.0036 0.0018 0.0031 0.0008 0.0006
Bud Site 0.0116 0.0104 0.0033 0.0018 0.0235 0 0.0141 0.0006 0.002 0.0035 0 0.0003 0.0033 0.0049 0.0017 0.0041 0.001 0.0003
Cell Periphery 0.0009 0.0002 0.0003 0.0002 0.0006 0 0.0006 0.0001 0.0001 0.0004 0 0 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001
Cytoplasm 0.1851 0.35 0.3613 0.477 0.4488 0.3793 0.1699 0.2948 0.2633 0.3287 0.3605 0.3997 0.256 0.3994 0.3707 0.355 0.3516 0.4448
Cytoplasmic Foci 0.0133 0.0067 0.0157 0.0225 0.0123 0.0078 0.0419 0.0057 0.0126 0.0145 0.0024 0.0104 0.0184 0.0168 0.0084 0.0082 0.0165 0.0147
Eisosomes 0.0005 0.0001 0.0004 0.0001 0.0002 0 0.0006 0.0001 0.0001 0.0004 0.0001 0 0.0002 0.0002 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0036 0.0015 0.0043 0.0045 0.0042 0.0022 0.004 0.0026 0.0027 0.0037 0.0016 0.0015 0.0016 0.0023 0.0016 0.0014 0.002 0.0016
Endosome 0.0206 0.0021 0.0049 0.0198 0.0238 0.0049 0.0235 0.0018 0.0165 0.021 0.0011 0.0083 0.0022 0.006 0.0019 0.0052 0.0074 0.0054
Golgi 0.0035 0.0002 0.0012 0.0061 0.0107 0.0001 0.0063 0.0002 0.0054 0.0057 0.0001 0.0033 0.0003 0.0003 0.0001 0.0004 0.0005 0.0003
Lipid Particles 0.0228 0.0002 0.0049 0.0112 0.0029 0.0006 0.0154 0.0002 0.0063 0.0176 0.0004 0.001 0.0009 0.0003 0.0002 0.0003 0.0004 0.0002
Mitochondria 0.0121 0.0003 0.0011 0.0076 0.0029 0.0003 0.007 0.0002 0.0059 0.0036 0.0003 0.0047 0.0023 0.0005 0.0006 0.0008 0.0005 0.0008
None 0.6624 0.6036 0.5739 0.3916 0.4062 0.5867 0.5859 0.6885 0.6483 0.5817 0.6285 0.5482 0.686 0.5459 0.5999 0.5848 0.6006 0.5016
Nuclear Periphery 0.0091 0.001 0.0015 0.005 0.0046 0.0018 0.0095 0.0007 0.0012 0.0012 0.0009 0.0009 0.0017 0.0013 0.0011 0.0015 0.0019 0.0014
Nucleolus 0.003 0.0012 0.0005 0.0006 0.0016 0.0003 0.0021 0.0001 0.0002 0.0004 0.0001 0.0006 0.0011 0.0004 0.0003 0.0005 0.0003 0.0004
Nucleus 0.0037 0.0103 0.0038 0.0306 0.0082 0.0074 0.013 0.0015 0.0025 0.0042 0.0022 0.0025 0.004 0.0075 0.0039 0.019 0.0071 0.009
Peroxisomes 0.0075 0.0003 0.014 0.0024 0.0014 0.0001 0.0192 0.0002 0.0085 0.0046 0.0001 0.0022 0.0007 0.0003 0.0004 0.0007 0.0005 0.0003
Punctate Nuclear 0.0051 0.0062 0.0033 0.0069 0.0058 0.0065 0.0096 0.0012 0.0014 0.0018 0.0007 0.0072 0.0056 0.003 0.0039 0.0036 0.0047 0.01
Vacuole 0.0041 0.0011 0.0014 0.0037 0.0034 0.0009 0.0037 0.0008 0.0015 0.0019 0.0004 0.0006 0.001 0.002 0.0008 0.0016 0.0015 0.0009
Vacuole Periphery 0.0013 0.0001 0.0002 0.0017 0.0009 0.0002 0.0016 0.0001 0.0004 0.0004 0.0001 0.0003 0.0002 0.0002 0.0001 0.0003 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.6675 30.0967 26.1254 28.5066 23.8847 41.9023 31.5791 31.3893 35.1973 27.3218
Translational Efficiency 0.8171 0.8735 0.8747 0.986 0.8621 0.6312 0.814 0.7864 0.7436 0.6834

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (60%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ura8

Ura8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ura8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available