Standard name
Human Ortholog
Description Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 0.96 0.97 0.95 0.86 0.85 0.81 0.82 0.73 0.83 1.0 1.0 0.99 0.91 0.95 0.96 0.97 0.93 0.91 0.93 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.12 0.13 0.2 0.16 0.34 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 2 0 1 0 0 0 0 0 0 0 0 2 2 0 2 3 7
Bud 2 1 1 2 1 2 7 4 4 1 4 4 0 0 0 0 0 0 0 2 2 2 9 10
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 7 6 1 3 7 3 5 8 2 9 5 1 2 4 1 6 1 0 0 1 0 0 0
Cytoplasm 332 540 345 210 284 320 373 352 263 244 233 281 513 662 691 32 108 50 334 524 318 450 566 626
Endoplasmic Reticulum 3 10 3 1 1 1 3 1 3 0 3 8 0 4 2 1 0 3 0 3 7 3 3 4
Endosome 0 2 0 0 0 1 3 6 4 4 4 1 1 1 1 1 0 0 0 3 3 3 11 9
Golgi 1 2 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 3 0 1 1 1
Mitochondria 2 0 0 3 1 32 52 53 66 48 109 49 0 0 0 0 0 0 1 4 6 4 6 4
Nucleus 0 2 1 1 0 0 11 2 0 4 3 4 0 0 0 0 1 2 0 0 0 4 0 0
Nuclear Periphery 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 1 0 2 2 0 1 5 0 0 0 0 1 0 0 1 1 1 8 5
Vac/Vac Membrane 0 0 1 3 6 4 6 19 16 21 14 8 2 1 2 2 4 0 0 8 2 3 3 3
Unique Cell Count 336 552 349 218 292 336 432 415 325 297 320 339 514 664 696 35 114 52 346 562 351 485 627 689
Labelled Cell Count 343 564 357 222 298 368 458 448 367 325 381 365 517 670 701 38 120 57 346 562 351 485 627 689


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.5 6.7 6.0 6.8 5.7 5.6 6.1 5.4 5.5 5.0 5.2 6.8 7.1 7.1 8.4 8.7 8.5 6.6 6.8 6.9
Std Deviation (1e-4) 0.5 0.9 1.2 1.1 1.5 1.3 1.2 1.4 1.7 1.2 1.0 1.2 0.9 1.1 1.3 1.6 1.5 1.5 1.3 1.3 1.3
Intensity Change (Log2) – – – -0.16 0.01 -0.24 -0.28 -0.14 -0.31 -0.3 -0.42 -0.37 0.0 0.08 0.08 0.32 0.36 0.33 -0.03 0.02 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.3 0 -0.6 0.7 0 1.0 -0.3 0 0 0 0 0 0
Cytoplasm -2.0 -1.5 -2.8 -6.4 -6.8 -7.8 -7.5 -9.8 -7.3 1.8 1.7 0.7 -3.1 -2.6 -1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1.6 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.9 6.7 6.9 8.9 7.8 11.9 7.4 0 0 0 0 0 0
Nucleus 0 0 0 2.6 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.7 3.8 4.7 3.6 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0379 1.567 1.3369 1.2775 0.8658 1.6948 1.6602 2.0754 1.7987 1.7704 1.6358 1.8294 1.6142 2.3876 1.8878 1.7386 1.8915 1.9926
Actin 0.0201 0.0037 0.0106 0.0014 0.0002 0.0065 0.0286 0.0058 0.0024 0.0005 0.0083 0.0021 0.0119 0.0009 0.0106 0.0003 0.0001 0.0017
Bud 0.0182 0.0319 0.038 0.0387 0.0011 0.0181 0.0009 0.0144 0.0158 0.0025 0.0156 0.0029 0.0015 0.0108 0.0097 0.0002 0.0001 0.0019
Bud Neck 0.0115 0.0007 0.0012 0.0007 0.0007 0.0046 0.0026 0.0007 0.0044 0.0009 0.0008 0.0057 0.0029 0.0003 0.0007 0.0004 0.0002 0.0034
Bud Periphery 0.0064 0.0084 0.0156 0.0104 0.0008 0.0061 0.0011 0.0068 0.0052 0.009 0.0202 0.0013 0.0025 0.004 0.0062 0.0001 0 0.0009
Bud Site 0.0141 0.0391 0.021 0.0098 0.0005 0.0025 0.0083 0.024 0.0163 0.002 0.0237 0.0003 0.0029 0.011 0.0142 0.0002 0.0001 0.0003
Cell Periphery 0.0008 0.0009 0.001 0.0005 0.0001 0.0004 0.0005 0.0011 0.0006 0.0004 0.0002 0.0001 0.0003 0.0002 0.0003 0 0 0.0001
Cytoplasm 0.3683 0.5605 0.4956 0.5676 0.6324 0.4946 0.3812 0.5743 0.4678 0.5089 0.6388 0.5961 0.6252 0.7786 0.6749 0.8004 0.741 0.8109
Cytoplasmic Foci 0.0385 0.0155 0.0188 0.0513 0.0513 0.0381 0.0374 0.0123 0.0228 0.0168 0.0204 0.0114 0.0141 0.0176 0.0114 0.0198 0.0064 0.0124
Eisosomes 0.0006 0.0002 0.0002 0.0001 0 0.0001 0.0005 0.0001 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.004 0.0021 0.0028 0.0028 0.001 0.0032 0.0039 0.0028 0.0025 0.0032 0.0026 0.0019 0.0049 0.0018 0.0029 0.0019 0.0023 0.0016
Endosome 0.0184 0.0059 0.0172 0.0287 0.0126 0.0206 0.0289 0.0034 0.027 0.0316 0.0088 0.0079 0.0121 0.0056 0.0054 0.0041 0.0027 0.0059
Golgi 0.0057 0.0005 0.0031 0.0016 0.0021 0.0074 0.0097 0.0006 0.0115 0.0013 0.0034 0.002 0.0024 0.0007 0.0014 0.0003 0.0001 0.0003
Lipid Particles 0.0067 0.0004 0.0031 0.0026 0.0089 0.0046 0.0265 0.0004 0.0124 0.0012 0.0016 0.0047 0.0029 0.0002 0.001 0.0001 0.0001 0.0001
Mitochondria 0.0193 0.003 0.0061 0.0042 0.0003 0.0128 0.0244 0.001 0.0044 0.0012 0.0077 0.0021 0.0031 0.0009 0.0019 0.0003 0.0002 0.0006
None 0.4222 0.3052 0.3252 0.1965 0.265 0.3442 0.4018 0.3379 0.3674 0.4003 0.2227 0.3457 0.2897 0.1562 0.2459 0.1641 0.2431 0.148
Nuclear Periphery 0.0056 0.0019 0.0022 0.0039 0.0007 0.0084 0.0075 0.0017 0.0035 0.0012 0.0014 0.0013 0.005 0.0012 0.0022 0.0008 0.0006 0.0016
Nucleolus 0.0061 0.0011 0.0041 0.0034 0.0008 0.0006 0.004 0.0011 0.0031 0.0006 0.0011 0.0004 0.0006 0.0004 0.0003 0.0001 0 0.0002
Nucleus 0.0064 0.0083 0.0123 0.025 0.0014 0.0095 0.0073 0.0052 0.008 0.0031 0.0023 0.0043 0.0057 0.0047 0.0043 0.0034 0.0016 0.004
Peroxisomes 0.0036 0.0008 0.0061 0.0011 0.0115 0.0037 0.008 0.0005 0.0097 0.0006 0.0155 0.001 0.0052 0.0009 0.0023 0.0005 0 0.0003
Punctate Nuclear 0.0198 0.0078 0.0103 0.0358 0.0073 0.01 0.0116 0.0036 0.0099 0.0007 0.0021 0.0077 0.004 0.0018 0.0027 0.0014 0.0005 0.0046
Vacuole 0.0027 0.0017 0.0039 0.0102 0.0009 0.0027 0.0033 0.0022 0.0038 0.0124 0.0022 0.0007 0.0023 0.0017 0.0014 0.0013 0.0009 0.0008
Vacuole Periphery 0.001 0.0003 0.0016 0.0037 0.0002 0.0014 0.002 0.0003 0.0013 0.0017 0.0005 0.0003 0.0006 0.0003 0.0003 0.0002 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.9397 9.544 9.9149 11.702 11.42 9.0699 15.0781 14.4916 12.3242 10.8981
Translational Efficiency 0.5563 0.5583 0.4077 0.4582 0.3633 0.4022 0.3865 0.2927 0.4315 0.4405

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
251 1400 247 443 981 1661 1903 97 1232 3061 2150 540

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 712.04 798.00 919.48 838.15 740.48 747.90 913.53 959.87 734.69 770.81 914.21 860.01
Standard Deviation 78.63 110.27 96.25 150.65 84.13 100.90 112.20 119.24 83.83 108.21 110.50 152.83
Intensity Change Log 2 — 0.164430 0.368860 0.235250 — 0.014385 0.302991 0.374378 — 0.089889 0.335657 0.307851

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000032 0.000434 0.000480 0.001260 0.000141 0.000359 0.000168 0.000215 0.000119 0.000393 0.000204 0.001072
Bud Neck 0.002566 0.006729 0.003271 0.005479 0.004706 0.020704 0.002432 0.002427 0.004270 0.014312 0.002528 0.004931
Bud Site 0.003743 0.010250 0.008729 0.029918 0.003258 0.019006 0.006694 0.012245 0.003357 0.015002 0.006928 0.026743
Cell Periphery 0.000200 0.000073 0.000212 0.000236 0.000109 0.000073 0.000079 0.000091 0.000127 0.000073 0.000095 0.000210
Cytoplasm 0.601916* 0.661772* 0.389285* 0.425382* 0.619297* 0.524773* 0.656252* 0.434151* 0.615756* 0.587432* 0.625582* 0.426957*
Cytoplasmic Foci 0.146465 0.124426 0.021272 0.032686 0.187991* 0.201193* 0.014716 0.065709 0.179531 0.166083 0.015469 0.038618
Eisosomes 0.000123 0.000042 0.000078 0.000070 0.000088 0.000071 0.000024 0.000062 0.000096 0.000057 0.000030 0.000069
Endoplasmic Reticulum 0.000510 0.000812 0.006709 0.004404 0.000886 0.000781 0.001486 0.001283 0.000809 0.000795 0.002086 0.003843
Endosome 0.003677 0.009279 0.005159 0.011093 0.009529 0.026497 0.001517 0.025231 0.008337 0.018622 0.001936 0.013633
Golgi 0.000617 0.001848 0.000108 0.005430 0.001364 0.006550 0.000054 0.000637 0.001212 0.004399 0.000061 0.004569
Lipid Particles 0.003381 0.002087 0.003008 0.001735 0.004802 0.003839 0.000734 0.001299 0.004513 0.003038 0.000995 0.001657
Mitochondria 0.000881 0.001051 0.000336 0.002545 0.001480 0.007925 0.000347 0.000085 0.001358 0.004781 0.000346 0.002103
Mitotic Spindle 0.000924 0.006325 0.000644 0.022101 0.003362 0.010854 0.002413 0.020234 0.002865 0.008783 0.002210 0.021766
None 0.005380 0.002972 0.002513 0.008680 0.003488 0.001300 0.003717 0.002711 0.003873 0.002065 0.003579 0.007608
Nuclear Periphery 0.000332 0.000285 0.001754 0.001659 0.000324 0.000232 0.000827 0.000588 0.000326 0.000256 0.000934 0.001467
Nuclear Periphery Foci 0.000368 0.000865 0.004493 0.003357 0.000797 0.000577 0.001407 0.003105 0.000710 0.000709 0.001761 0.003312
Nucleolus 0.000428 0.000689 0.000327 0.000485 0.000880 0.000947 0.000140 0.000158 0.000788 0.000829 0.000162 0.000426
Nucleus 0.191715* 0.095907 0.393601* 0.256153* 0.103693 0.048010 0.220411* 0.054022 0.121626 0.069917 0.240308* 0.219845*
Peroxisomes 0.003630 0.003094 0.000341 0.000402 0.002557 0.009266 0.000150 0.000841 0.002776 0.006443 0.000172 0.000481
Vacuole 0.032657 0.069660 0.157478 0.181146 0.050434 0.113966 0.086322 0.374485* 0.046813 0.093702 0.094496 0.215876*
Vacuole Periphery 0.000455 0.001399 0.000201 0.005777 0.000813 0.003080 0.000108 0.000420 0.000740 0.002311 0.000119 0.004815

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.90 -10.67 -2.24 -1.05 -0.61 -4.11 -0.93 -2.46 1.35 -1.80 -2.69 -3.17 -2.10 -1.17 -1.73
Bud Neck -5.76 -1.97 -3.24 1.54 -1.38 -10.75 2.81 2.49 12.90 0.03 -10.42 2.50 -0.58 9.66 -3.08
Bud Site -3.04 -1.38 -3.70 -2.24 -2.64 -10.76 -2.17 -2.79 2.59 -1.91 -10.72 -2.38 -4.48 -0.93 -3.68
Cell Periphery 8.49 -1.20 0.91 -5.39 1.85 6.37 5.71 1.34 -2.13 -1.69 8.93 5.81 -2.28 -5.87 -4.55
Cytoplasm -3.76 13.77 14.21 22.00 -0.77 8.86 5.18 8.39 5.30 6.78 3.38 8.52 20.11 18.99 14.95
Cytoplasmic Foci 2.48 15.95 15.25 22.33 -1.64 -1.27 30.08 19.40 22.45 -3.09 2.69 33.59 29.72 36.87 -4.14
Eisosomes 9.06 2.91 5.01 -6.50 1.73 3.38 11.68 3.62 1.01 -6.33 7.93 13.17 3.54 -4.84 -10.97
Endoplasmic Reticulum -1.83 -11.27 -4.71 -4.40 3.80 0.44 -4.21 -2.32 -2.52 -0.04 0.12 -8.57 -4.58 -4.64 -2.03
Endosome -6.95 -2.37 -1.81 2.32 -0.62 -9.83 11.37 0.83 5.54 -1.81 -9.37 11.10 1.75 8.39 -3.71
Golgi -3.04 2.07 -1.48 -0.25 -2.05 -6.15 5.64 4.71 7.89 -1.62 -6.30 6.00 -0.64 2.83 -2.14
Lipid Particles 2.70 0.44 3.09 0.17 3.53 1.33 7.92 6.85 3.65 -3.80 2.84 8.30 6.28 2.80 -6.13
Mitochondria -0.26 0.88 -0.75 -0.81 -2.36 -5.00 2.50 3.05 6.59 7.26 -4.44 2.66 0.37 4.80 -2.33
Mitotic Spindle -2.89 -0.30 -3.31 -1.78 -3.31 -3.36 0.15 -0.81 1.29 -0.95 -3.56 -0.04 -2.84 -1.07 -2.97
None 1.60 2.05 -0.31 -2.01 -2.47 2.93 0.26 0.63 -1.85 0.60 2.74 1.02 -1.08 -2.70 -1.77
Nuclear Periphery 0.71 -14.94 -11.19 -11.81 -1.51 1.11 -11.54 -6.00 -7.99 1.75 1.03 -15.16 -11.66 -12.73 -5.67
Nuclear Periphery Foci -3.75 -8.99 -2.36 -1.74 2.69 1.57 -6.35 -5.24 -5.63 -3.22 0.11 -9.99 -2.56 -2.57 -0.60
Nucleolus -2.95 2.01 0.73 3.33 -1.03 -0.31 3.75 3.53 3.80 -0.68 -0.26 3.96 2.81 4.08 -4.27
Nucleus 7.55 -10.04 -5.31 -13.96 5.55 13.18 -22.31 3.11 -2.27 15.51 11.60 -21.81 -10.36 -15.16 0.93
Peroxisomes 0.16 1.08 1.08 5.42 -0.23 -6.40 5.12 4.54 9.64 -2.70 -3.97 3.67 3.36 10.95 -2.59
Vacuole -7.76 -12.09 -17.78 -15.44 -3.81 -16.58 -19.50 -13.99 -11.78 -10.55 -16.71 -22.75 -21.14 -17.26 -11.90
Vacuole Periphery -2.66 1.56 -1.66 -0.81 -1.91 -4.61 4.39 3.88 6.37 -1.12 -4.89 4.70 -1.28 0.37 -2.00
Standard name
Human Ortholog
Description Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family
Localization
Cell Percentages cytoplasm (69%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ymr1

Ymr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available