Standard name
Human Ortholog
Description Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.29 0.21 0 0.07 0.15 0 0.07 0.07
Bud 0.05 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.34 0.26 0.27 0 0 0 0 0 0
Cytoplasm 0.7 0.91 0.87 0.87 0.8 0.68 0.67 0.61 0.57 0.6 0.47 0.53 0.92 0.92 0.96 0.46 0.39 0.45 0.49 0.39 0.24 0.34 0.4 0.37
Endoplasmic Reticulum 0.15 0.14 0.07 0.06 0.07 0.08 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0.15 0.16 0.28 0.15 0.16 0.15
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.07 0.1 0.35 0.43 0.42 0.51 0.49 0.67 0.6 0 0 0 0.07 0.08 0.09 0.21 0.11 0.17 0.24 0.16 0.13
Nucleus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0.07 0.11 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 2 0 3 0 3 4 2 1 0 2 7 1 31 49 35 1 4 27 7 14 23
Bud 1 0 3 2 4 9 7 14 12 5 10 11 1 2 1 1 6 2 0 0 3 5 3 9
Bud Neck 0 0 0 0 0 0 1 0 0 1 0 0 0 3 2 0 2 1 1 2 4 7 8 11
Bud Site 0 0 0 0 0 0 1 1 1 0 0 1 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 1 0 8 8 6 8 17 7 7 4 10 4 10 13 50 44 46 0 0 6 6 2 7
Cytoplasm 14 20 140 118 209 154 294 305 145 219 100 175 212 312 364 68 67 77 25 26 44 61 81 119
Endoplasmic Reticulum 3 3 11 8 18 19 18 15 8 11 9 8 15 18 9 2 6 7 7 11 51 27 31 46
Endosome 0 1 2 1 11 1 3 5 3 2 0 1 1 1 1 1 2 1 0 2 3 2 0 8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 3 1 2 2 1 1 4
Mitochondria 0 0 8 9 27 79 190 210 131 179 143 198 0 0 0 10 13 15 10 7 30 44 31 41
Nucleus 1 0 1 0 2 1 3 4 0 5 1 1 0 1 1 3 0 1 0 0 0 1 0 1
Nuclear Periphery 1 0 0 0 0 0 0 3 0 2 0 2 0 0 0 0 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 5 0 0 1 0 1 2 0 0 0 0 0 0 3 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 5 8 0 1 0 3 0 0 0 1 3 1 0 2 0 0 0 3
Vac/Vac Membrane 1 0 1 2 7 4 2 14 7 11 2 5 0 2 3 1 7 14 1 3 4 13 22 33
Unique Cell Count 20 22 161 135 261 226 441 496 255 368 213 332 231 340 381 147 170 170 52 69 182 182 201 318
Labelled Cell Count 21 25 170 151 292 276 532 600 318 447 272 416 236 356 395 169 201 206 52 69 182 182 201 318


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.9 4.8 4.9 4.8 4.3 3.8 4.0 3.6 3.6 3.6 3.5 6.2 5.8 5.9 8.4 8.2 8.3 6.5 6.6 6.6
Std Deviation (1e-4) 0.4 1.6 2.1 2.3 2.1 1.7 1.9 1.5 0.9 1.3 1.3 1.4 1.3 1.2 1.2 1.6 1.6 1.5 1.7 1.9 1.9
Intensity Change (Log2) – – – 0.01 -0.02 -0.17 -0.36 -0.28 -0.43 -0.42 -0.42 -0.46 0.36 0.26 0.28 0.81 0.76 0.79 0.42 0.44 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 5.4 6.7 5.3
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 8.1 6.9 7.1
Cytoplasm 0.1 -1.8 -4.3 -4.9 -6.0 -6.4 -6.2 -8.0 -7.4 1.6 1.7 3.6 -7.6 -8.9 -8.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 -0.1 -0.7 -2.5 -2.4 -1.4 -1.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0.7 1.0 1.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.059 0.0037 0.0366 0.1334 0.0553 0.0069 0.0485 0.0018 0.0345 0.0698 0.0078 0.0219 0.005 0.0019 0.0141 0 0.0002 0.0004
Bud 0.0007 0.0008 0.0007 0.0002 0.0016 0.0003 0.0005 0.0002 0.0006 0.012 0.0003 0.0009 0.0014 0.0001 0.0005 0 0 0
Bud Neck 0.0065 0.0012 0.0068 0.0051 0.0175 0.0098 0.0018 0.0012 0.0027 0.0015 0.0041 0.0072 0.0002 0.0006 0.0472 0 0.001 0.0031
Bud Periphery 0.0017 0.0019 0.0025 0.0005 0.0061 0.0011 0.0007 0.0003 0.0016 0.0112 0.0008 0.0027 0.0048 0.0004 0.0024 0 0 0.0001
Bud Site 0.0055 0.0357 0.0157 0.0017 0.0061 0.0034 0.0026 0.0201 0.0087 0.0638 0.0041 0.003 0.0018 0.0044 0.0102 0 0 0.0001
Cell Periphery 0.0185 0.0179 0.0233 0.0052 0.0112 0.0051 0.0042 0.0075 0.011 0.0013 0.0057 0.0041 0.0029 0.0059 0.0659 0 0.0011 0.0021
Cytoplasm 0.2345 0.3754 0.3578 0.3215 0.3745 0.3235 0.2085 0.3919 0.3573 0.237 0.5266 0.43 0.2787 0.4104 0.551 0.0016 0.4407 0.432
Cytoplasmic Foci 0.0248 0.0232 0.0568 0.0096 0.0231 0.0241 0.0477 0.0337 0.0305 0.0219 0.0168 0.0354 0.0179 0.0237 0.0139 0.0003 0.0042 0.0081
Eisosomes 0.0044 0.001 0.003 0.0041 0.0024 0.0005 0.0016 0.0005 0.001 0.0015 0.0003 0.0004 0.0006 0.0003 0.0031 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.1202 0.1178 0.1196 0.3492 0.2004 0.1525 0.0569 0.0449 0.067 0.0123 0.1018 0.0811 0.0203 0.0513 0.0552 0.0001 0.0057 0.2753
Endosome 0.0257 0.0101 0.022 0.0036 0.0469 0.0214 0.0235 0.0248 0.0144 0.043 0.0276 0.0094 0.0152 0.0094 0.0037 0 0.0004 0.0082
Golgi 0.0108 0.0051 0.0099 0.0053 0.0106 0.006 0.0079 0.0093 0.0087 0.0438 0.0163 0.0064 0.0038 0.0015 0.0046 0 0.0001 0.0022
Lipid Particles 0.0273 0.008 0.0381 0.0019 0.0106 0.0158 0.0415 0.0281 0.0118 0.0251 0.0103 0.0069 0.0172 0.0301 0.0101 0.0001 0.0005 0.0242
Mitochondria 0.0021 0.0034 0.0032 0.0005 0.0076 0.0014 0.0015 0.0018 0.0028 0.0563 0.0013 0.0049 0.0981 0.0001 0.0006 0 0.0001 0.0003
None 0.4239 0.38 0.2829 0.1526 0.1709 0.4194 0.5227 0.4254 0.4344 0.3159 0.257 0.3767 0.5133 0.429 0.2095 0.9973 0.5445 0.2392
Nuclear Periphery 0.0148 0.0026 0.0036 0.0013 0.0215 0.0019 0.0062 0.0012 0.0013 0.0029 0.0074 0.0008 0.0009 0.0016 0.0015 0.0001 0.0001 0.0014
Nucleolus 0.0007 0.001 0.0008 0.0002 0.0021 0.0002 0.0005 0.0003 0.0005 0.0008 0.0005 0.0003 0.0021 0.0003 0.0003 0 0.0001 0
Nucleus 0.0036 0.0021 0.0022 0.0005 0.0112 0.0012 0.0103 0.0008 0.0008 0.0034 0.0025 0.0007 0.0015 0.001 0.0013 0 0.0004 0.0006
Peroxisomes 0.0064 0.0013 0.0049 0.0009 0.0008 0.0005 0.0051 0.0007 0.0048 0.0661 0.0008 0.0026 0.004 0.0002 0.0003 0 0 0.0001
Punctate Nuclear 0.0035 0.0029 0.0025 0.0004 0.0038 0.0014 0.0036 0.0008 0.001 0.0011 0.0038 0.0016 0.0008 0.0008 0.0003 0.0001 0.0001 0.0002
Vacuole 0.0044 0.0045 0.0067 0.0022 0.013 0.0032 0.0039 0.0044 0.0043 0.0073 0.0035 0.0026 0.0084 0.0264 0.0039 0 0.0005 0.0022
Vacuole Periphery 0.0011 0.0004 0.0006 0.0001 0.0028 0.0004 0.0004 0.0003 0.0003 0.002 0.0008 0.0004 0.0009 0.0005 0.0003 0 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 65.8076 33.8462 23.9194 39.6039 36.2328 50.4546 40.333 36.846 42.4855 52.9113
Translational Efficiency 1.2231 1.6791 1.868 1.2611 1.78 1.4574 1.2932 1.1219 1.1411 1.3451

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1413 163 2874 1456 1658 1227 2407 246 3071 1390 5281 1702

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 590.98 769.34 900.75 871.20 722.91 703.38 815.36 942.82 662.21 711.11 861.83 881.55
Standard Deviation 66.93 98.89 113.23 109.12 98.43 102.54 140.83 103.36 107.78 104.30 133.51 111.19
Intensity Change Log 2 — 0.380512 0.608017 0.559895 — -0.039512 0.173621 0.383166 — 0.164639 0.385298 0.465346

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001167 0.004394 0.005303 0.006020 0.001098 0.003909 0.006086 0.003990 0.001130 0.003966 0.005660 0.005727
Bud Neck 0.022553 0.053478 0.013131 0.016483 0.014232 0.047511 0.020349 0.015430 0.018060 0.048210 0.016421 0.016331
Bud Site 0.005501 0.041539 0.026464 0.065854 0.004298 0.027129 0.035793 0.048665 0.004852 0.028819 0.030716 0.063369
Cell Periphery 0.001485 0.000946 0.001832 0.001093 0.000580 0.001177 0.001281 0.001047 0.000996 0.001150 0.001581 0.001086
Cytoplasm 0.410834* 0.287137* 0.399049* 0.411237* 0.454346* 0.367245* 0.465711* 0.386681* 0.434326* 0.357851* 0.429432* 0.407688*
Cytoplasmic Foci 0.250855* 0.286841* 0.033735 0.047543 0.222870* 0.250366* 0.061132 0.068056 0.235746* 0.254643* 0.046222 0.050508
Eisosomes 0.005098 0.001760 0.000922 0.000398 0.001800 0.002257 0.000648 0.000299 0.003317 0.002199 0.000797 0.000383
Endoplasmic Reticulum 0.018274 0.007464 0.127348* 0.022839 0.019846 0.011663 0.051909 0.011348 0.019123 0.011171 0.092964 0.021178
Endosome 0.009027 0.040266 0.005867 0.012827 0.014649 0.033649 0.007264 0.006909 0.012062 0.034425 0.006504 0.011971
Golgi 0.019193 0.009507 0.002167 0.004611 0.010810 0.016984 0.002469 0.001250 0.014667 0.016108 0.002305 0.004125
Lipid Particles 0.071528 0.027619 0.023975 0.010602 0.044028 0.037593 0.020430 0.008389 0.056681 0.036424 0.022359 0.010282
Mitochondria 0.024780 0.006570 0.001737 0.006838 0.004684 0.011950 0.002588 0.005306 0.013930 0.011319 0.002125 0.006616
Mitotic Spindle 0.000549 0.001003 0.002362 0.022916 0.000561 0.000828 0.004781 0.026995 0.000556 0.000849 0.003465 0.023506
None 0.004756 0.000532 0.001914 0.002488 0.001940 0.001467 0.004819 0.001990 0.003236 0.001358 0.003238 0.002416
Nuclear Periphery 0.000838 0.000318 0.006460 0.002756 0.000835 0.000577 0.002157 0.001804 0.000836 0.000547 0.004499 0.002619
Nuclear Periphery Foci 0.000424 0.001380 0.011044 0.006321 0.000886 0.000544 0.006063 0.004674 0.000673 0.000642 0.008774 0.006083
Nucleolus 0.000632 0.000682 0.000333 0.001194 0.000784 0.001061 0.000678 0.000444 0.000714 0.001016 0.000490 0.001085
Nucleus 0.110716 0.073797 0.258346* 0.153005* 0.122684 0.072181 0.195811* 0.181301* 0.117177 0.072371 0.229843* 0.157095*
Peroxisomes 0.018382 0.023697 0.002777 0.004041 0.008381 0.015624 0.005207 0.009226 0.012983 0.016570 0.003884 0.004790
Vacuole 0.021869 0.129145 0.074988 0.199294 0.069722 0.093605 0.104283 0.215952* 0.047704 0.097773 0.088341 0.201702*
Vacuole Periphery 0.001538 0.001924 0.000244 0.001640 0.000968 0.002677 0.000541 0.000244 0.001230 0.002589 0.000379 0.001438

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.48 -5.44 -2.59 0.61 1.54 -3.27 -6.16 -1.36 1.76 3.57 -3.67 -8.14 -2.81 0.77 2.36
Bud Neck -3.50 5.80 5.59 4.77 0.62 -10.98 -3.19 1.36 9.65 3.13 -10.60 1.94 4.44 12.54 3.17
Bud Site -3.93 -9.17 -9.96 -0.02 -3.79 -8.40 -12.33 -3.56 -0.05 0.70 -9.11 -15.19 -10.90 -2.49 -2.95
Cell Periphery 1.92 -0.99 3.69 0.91 5.01 -3.12 -3.59 0.18 2.40 2.46 -0.80 -3.30 2.35 2.44 4.89
Cytoplasm 6.51 2.15 4.35 -4.23 2.82 9.33 0.75 6.46 1.77 6.16 9.65 2.73 8.70 -0.69 7.04
Cytoplasmic Foci -1.94 42.61 40.93 16.32 -0.89 -3.74 33.20 25.91 26.48 1.26 -2.86 53.99 53.09 38.62 3.50
Eisosomes 4.56 6.61 7.61 3.60 5.92 -1.63 7.52 10.11 8.59 6.02 3.04 8.33 9.97 8.95 7.23
Endoplasmic Reticulum 3.61 -20.30 1.65 -2.25 21.73 3.12 -7.29 3.57 1.07 9.97 3.91 -20.84 2.80 -0.89 22.39
Endosome -5.26 5.06 1.06 5.41 -2.56 -9.99 8.97 9.46 15.52 1.76 -12.81 10.41 5.25 14.82 -1.97
Golgi 4.64 15.75 13.39 3.38 -0.34 -3.76 9.51 10.99 11.60 4.17 -0.94 17.84 14.06 10.24 0.09
Lipid Particles 6.57 14.39 19.71 3.20 12.20 2.00 9.93 16.08 13.08 8.81 7.14 17.12 24.88 13.26 14.09
Mitochondria 6.86 13.15 10.57 1.17 -2.02 -5.36 4.26 1.64 5.23 -0.02 1.88 13.62 8.64 5.49 -1.74
Mitotic Spindle -0.69 -3.07 -7.61 -7.48 -6.80 -0.52 -5.07 -2.52 -2.47 -1.78 -0.58 -5.91 -8.08 -7.91 -6.72
None 5.25 3.41 2.81 -5.14 -1.24 1.15 -2.85 -0.45 -1.77 2.62 4.17 0.59 1.75 -3.13 1.12
Nuclear Periphery 3.39 -12.25 -8.17 -10.83 6.26 1.33 -6.57 -4.31 -4.64 -0.80 1.68 -13.86 -9.54 -9.48 4.02
Nuclear Periphery Foci -4.22 -14.66 -8.86 -6.24 6.72 3.42 -9.89 -5.86 -6.48 2.12 0.33 -17.51 -9.38 -9.42 5.92
Nucleolus -0.47 5.96 -0.72 -0.31 -1.72 -1.21 0.22 1.98 3.08 1.94 -1.78 2.00 -0.27 0.81 -1.02
Nucleus 3.65 -27.94 -10.33 -9.38 13.56 11.55 -16.31 -5.31 -9.06 0.99 12.11 -31.71 -11.29 -18.75 10.99
Peroxisomes -1.34 13.83 13.64 5.57 0.67 -5.91 4.35 2.21 5.60 0.29 -3.01 13.65 13.64 12.79 1.98
Vacuole -9.32 -23.81 -36.48 -10.78 -26.64 -5.25 -13.65 -14.46 -12.94 -11.08 -12.85 -22.61 -36.08 -25.80 -27.12
Vacuole Periphery -0.63 10.76 3.10 1.95 -3.26 -6.17 4.62 8.12 9.49 4.76 -5.41 11.23 2.69 6.36 -2.51

Micrographs


Penetrance and Abundance Data

R1 R2
30C 30C
Mean Penetrance 0.164369 0.011220
Mean Abundance 654.60 643.02
Cell Count 248 108
Standard name
Human Ortholog
Description Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
Localization
Cell Percentages ER (24%), cytoplasm (3%), mixed (40%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ilm1

Ilm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ilm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available