Standard name
Human Ortholog
Description Protein involved in incorporating iron-sulfur clusters into proteins; mitochondrial matrix protein; involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0.05 0 0 0 0 0.05 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.22 0.46 0.45 0.24 0.32 0.16 0.19 0.2 0.17 0.13 0.12 0.19 0.51 0.63 0.64 0.38 0.34 0.32 0.05 0.12 0 0.12 0.1 0.14
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Endosome 0 0.16 0 0 0 0 0 0 0 0 0 0 0.13 0.13 0.15 0.16 0.14 0.19 0.09 0.1 0.07 0 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.85 0.26 0.6 0.8 0.67 0.88 0.83 0.81 0.85 0.89 0.9 0.89 0.17 0.13 0.12 0.18 0.1 0.12 0.72 0.59 0.7 0.67 0.61 0.49
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.08 0.1 0.07 0.06 0 0 0 0 0 0
SpindlePole 0 0.06 0 0.05 0 0 0.08 0.05 0 0.06 0 0 0 0 0 0.22 0.15 0.22 0 0 0 0 0 0.05
Vac/Vac Membrane 0.06 0.11 0 0 0.07 0 0 0 0 0 0 0 0.11 0.07 0.1 0.11 0.22 0.12 0 0 0 0 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 5 0 1 0 0 0 0 0 0 0 4 0 0 0 1 5 4 11 7 6 10 19
Bud 4 6 8 3 2 3 12 20 12 4 2 11 6 8 6 7 8 12 0 4 0 4 9 9
Bud Neck 0 0 2 1 1 1 0 2 0 0 1 0 1 0 0 4 6 2 2 6 0 4 8 15
Bud Site 1 0 0 1 0 0 0 3 6 5 6 3 0 1 0 2 0 2 – – – – – –
Cell Periphery 1 4 2 0 0 2 1 1 2 3 1 6 8 6 9 11 15 14 0 0 0 2 3 3
Cytoplasm 57 133 66 20 52 29 60 85 36 30 19 39 162 239 307 157 131 109 13 49 8 48 47 57
Endoplasmic Reticulum 6 7 3 3 3 6 0 0 1 3 2 0 4 1 2 15 24 20 2 2 2 2 5 6
Endosome 1 46 1 0 3 1 0 1 0 0 0 0 41 50 71 66 55 64 24 39 12 13 25 20
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 0 4 4 3 5 0 1 6 1
Mitochondria 224 76 87 67 109 160 266 343 179 201 144 185 55 49 58 73 38 41 199 236 130 269 281 205
Nucleus 1 0 1 0 0 1 2 12 2 0 2 1 0 0 1 9 10 8 0 1 0 0 2 0
Nuclear Periphery 2 0 0 1 1 1 0 0 1 0 0 0 0 0 0 1 0 0 1 1 0 0 2 1
Nucleolus 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1 2 1 0 0 0 0 3 1
Peroxisomes 0 12 2 0 2 2 1 1 0 1 1 0 26 31 39 41 26 21 3 4 4 4 4 4
SpindlePole 8 16 6 4 3 6 27 20 9 13 3 4 14 12 14 91 57 76 7 9 4 15 19 19
Vac/Vac Membrane 17 31 0 1 12 6 5 6 5 4 2 2 34 27 49 45 83 42 7 16 5 16 24 39
Unique Cell Count 264 289 146 84 162 182 320 422 211 226 160 207 317 377 481 411 380 340 277 400 187 401 461 416
Labelled Cell Count 323 334 183 101 189 218 374 495 253 265 183 251 356 424 556 523 460 421 277 400 187 401 461 416


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.4 3.7 3.4 3.7 3.0 2.8 3.2 3.1 2.7 2.9 2.9 4.9 4.5 4.6 5.2 6.5 6.2 3.8 4.0 4.7
Std Deviation (1e-4) 0.6 0.9 0.8 0.9 1.2 0.6 1.0 1.1 1.3 0.9 1.0 1.3 1.0 0.9 1.1 1.6 2.1 1.9 0.7 1.2 1.0
Intensity Change (Log2) – – – -0.13 -0.01 -0.3 -0.38 -0.21 -0.25 -0.43 -0.36 -0.35 0.42 0.28 0.32 0.5 0.82 0.76 0.03 0.12 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.2 6.2 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7942 4.2368 4.4601 4.2683 3.8452 4.9662 3.6666 2.9737 2.8218 3.0167 2.1308 2.7268 3.6906 4.2371 4.1182 4.0325 3.7908 4.0308
Actin 0.0318 0.0083 0.015 0.0088 0.0071 0.0085 0.0465 0.0128 0.0239 0.0142 0.0347 0.0178 0.0051 0.0118 0.0157 0.0042 0.0062 0.0191
Bud 0.0026 0.0041 0.0031 0.0064 0.0022 0.0015 0.0024 0.0014 0.0016 0.0025 0.0047 0.0008 0.0012 0.012 0.0082 0.0009 0.0086 0.0013
Bud Neck 0.0057 0.0015 0.0025 0.0008 0.0008 0.0125 0.0034 0.0019 0.0075 0.004 0.001 0.0016 0.0019 0.0021 0.006 0.001 0.0012 0.0043
Bud Periphery 0.0071 0.0074 0.0088 0.0404 0.0062 0.0051 0.0041 0.0025 0.0033 0.0079 0.0253 0.0045 0.0045 0.0093 0.011 0.0021 0.0423 0.0043
Bud Site 0.0059 0.0364 0.0155 0.0028 0.0034 0.0039 0.012 0.0428 0.0243 0.0029 0.0022 0.0013 0.0076 0.0486 0.0307 0.0014 0.0056 0.0016
Cell Periphery 0.0047 0.0018 0.0026 0.0012 0.0021 0.0023 0.0011 0.0012 0.0017 0.0014 0.0035 0.0021 0.0022 0.0028 0.0023 0.0005 0.0101 0.01
Cytoplasm 0.0205 0.0392 0.0292 0.0099 0.0215 0.0173 0.0409 0.0238 0.0404 0.0236 0.0146 0.0194 0.0131 0.0288 0.024 0.0187 0.0381 0.019
Cytoplasmic Foci 0.0506 0.0527 0.0464 0.0175 0.0418 0.0527 0.1157 0.1075 0.0637 0.0403 0.0669 0.0681 0.0736 0.0874 0.0876 0.0693 0.1044 0.0505
Eisosomes 0.0018 0.0006 0.0009 0.0004 0.0003 0.0009 0.0008 0.0007 0.0015 0.0004 0.001 0.0008 0.0008 0.0008 0.0009 0.0006 0.0004 0.0008
Endoplasmic Reticulum 0.0055 0.006 0.0035 0.001 0.0019 0.0023 0.0104 0.0021 0.0035 0.0022 0.0013 0.0013 0.0103 0.0083 0.0026 0.0054 0.0013 0.0029
Endosome 0.1417 0.1526 0.0842 0.0381 0.0855 0.0793 0.1432 0.1304 0.0722 0.1583 0.0885 0.0582 0.1618 0.1399 0.0872 0.0626 0.0952 0.1076
Golgi 0.0688 0.0658 0.0592 0.0268 0.0638 0.0538 0.0713 0.078 0.0647 0.0912 0.0714 0.0626 0.0589 0.0638 0.0418 0.0286 0.0754 0.0713
Lipid Particles 0.0497 0.0148 0.0253 0.0046 0.0133 0.0217 0.0334 0.033 0.0158 0.0082 0.0058 0.0205 0.0903 0.0164 0.0141 0.013 0.0264 0.0273
Mitochondria 0.4801 0.5149 0.6033 0.7892 0.7089 0.6364 0.3134 0.4533 0.5152 0.5425 0.6005 0.5803 0.4575 0.4214 0.5532 0.7161 0.5171 0.5771
None 0.0263 0.0195 0.0342 0.0082 0.0079 0.041 0.071 0.0477 0.1221 0.0162 0.0144 0.1097 0.0253 0.0481 0.0294 0.0192 0.0347 0.0441
Nuclear Periphery 0.0166 0.0056 0.0054 0.0005 0.0004 0.0059 0.0179 0.0006 0.0008 0.0011 0.0002 0.0002 0.0158 0.0053 0.0009 0.0056 0.0004 0.0049
Nucleolus 0.0025 0.0013 0.001 0.0003 0.0006 0.001 0.0012 0.0008 0.0008 0.0003 0.0002 0.0004 0.0012 0.0054 0.0013 0.0008 0.001 0.0004
Nucleus 0.0127 0.0029 0.0016 0.0007 0.001 0.0018 0.0125 0.0007 0.0007 0.0007 0.0002 0.0003 0.0012 0.0036 0.001 0.0014 0.0012 0.0007
Peroxisomes 0.0337 0.0284 0.0427 0.0199 0.0178 0.0416 0.0733 0.0472 0.029 0.0483 0.0551 0.0447 0.0472 0.0522 0.0635 0.033 0.0113 0.038
Punctate Nuclear 0.0073 0.0011 0.002 0.0002 0.0003 0.0012 0.007 0.0007 0.0007 0.0002 0.0002 0.0004 0.0015 0.0047 0.001 0.0018 0.003 0.0004
Vacuole 0.0166 0.027 0.0072 0.0155 0.009 0.0047 0.0118 0.0071 0.0039 0.021 0.0052 0.0032 0.0121 0.0212 0.0131 0.0088 0.009 0.0075
Vacuole Periphery 0.0079 0.0081 0.0061 0.0068 0.0043 0.0045 0.0064 0.0036 0.0027 0.0125 0.0031 0.0018 0.0069 0.0063 0.0042 0.0049 0.0071 0.007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.8627 9.2432 3.4294 11.0809 6.703 10.3341 15.8544 14.643 16.3454 15.4706
Translational Efficiency 0.5888 0.6302 1.5538 0.5082 0.6427 0.6714 0.5892 0.6138 0.5269 0.5131

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1468 1268 907 1561 1354 2280 835 794 2822 3548 1742 2355

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 646.32 613.32 785.45 780.05 685.60 718.49 795.84 1022.25 665.17 680.90 790.43 861.71
Standard Deviation 65.79 84.87 98.63 97.77 78.86 102.07 118.09 148.10 74.97 108.67 108.52 163.83
Intensity Change Log 2 — -0.075609 0.281271 0.271318 — 0.067601 0.215111 0.576309 — -0.000119 0.247595 0.436332

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000501 0.000959 0.000420 0.007801 0.000286 0.000743 0.001204 0.002906 0.000398 0.000820 0.000795 0.006151
Bud Neck 0.047440 0.043440 0.024625 0.039681 0.031750 0.021612 0.017383 0.063487 0.039912 0.029413 0.021153 0.047707
Bud Site 0.015815 0.048628 0.009216 0.065656 0.010392 0.017106 0.015970 0.077046 0.013213 0.028372 0.012453 0.069497
Cell Periphery 0.000205 0.000511 0.000309 0.000239 0.000281 0.000303 0.002488 0.000699 0.000242 0.000377 0.001354 0.000394
Cytoplasm 0.159107* 0.051733 0.092915 0.372865* 0.063204 0.028011 0.359854* 0.528055* 0.113093* 0.036489 0.220868* 0.425188*
Cytoplasmic Foci 0.347724* 0.145776 0.118148 0.077560 0.232012* 0.116960 0.106092 0.017362 0.292205* 0.127258 0.112369 0.057264
Eisosomes 0.000299 0.000157 0.000204 0.000150 0.000190 0.000122 0.002751 0.000049 0.000247 0.000134 0.001425 0.000116
Endoplasmic Reticulum 0.001821 0.000965 0.006771 0.003272 0.002409 0.000653 0.004412 0.000805 0.002103 0.000765 0.005640 0.002440
Endosome 0.082551 0.142268 0.063638 0.008647 0.078638 0.115871 0.014851 0.002860 0.080673 0.125305 0.040253 0.006696
Golgi 0.027256 0.107803 0.009439 0.004113 0.063620 0.110648* 0.003494 0.001454 0.044703 0.109631 0.006590 0.003217
Lipid Particles 0.028909 0.011681 0.062462 0.002878 0.040338 0.008907 0.017571 0.000756 0.034393 0.009898 0.040945 0.002162
Mitochondria 0.123164* 0.282738* 0.063026 0.007724 0.306800* 0.430590* 0.006737 0.015428 0.211273* 0.377750* 0.036045 0.010321
Mitotic Spindle 0.007127 0.016697 0.043150 0.030536 0.007614 0.016870 0.024452 0.035660 0.007361 0.016808 0.034187 0.032264
None 0.001653 0.003244 0.007706 0.001795 0.004235 0.001547 0.020739 0.005727 0.002892 0.002154 0.013953 0.003121
Nuclear Periphery 0.000632 0.000327 0.012328 0.001270 0.001647 0.000428 0.002528 0.001246 0.001119 0.000392 0.007631 0.001262
Nuclear Periphery Foci 0.000393 0.000428 0.005608 0.001099 0.000583 0.000330 0.001329 0.000087 0.000484 0.000365 0.003557 0.000757
Nucleolus 0.002981 0.005224 0.032489 0.000950 0.007710 0.002529 0.005313 0.001416 0.005250 0.003492 0.019462 0.001107
Nucleus 0.044605 0.013302 0.265201* 0.183787* 0.034230 0.009102 0.274567* 0.181374* 0.039627 0.010603 0.269690* 0.182973*
Peroxisomes 0.039602 0.060612 0.021728 0.037381 0.048982 0.043794 0.019039 0.005747 0.044103 0.049804 0.020439 0.026715
Vacuole 0.058287 0.043817 0.134596 0.151663 0.046735 0.053552 0.097344 0.056322 0.052745 0.050073 0.116740 0.119518
Vacuole Periphery 0.009928 0.019690 0.026021 0.000934 0.018344 0.020322 0.001883 0.001516 0.013966 0.020096 0.014451 0.001130

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.16 0.12 -6.68 -6.11 -6.74 -4.70 -5.81 -2.29 -1.75 -1.08 -4.75 -4.73 -7.01 -6.29 -6.17
Bud Neck 0.96 6.71 3.99 2.45 -3.10 3.82 5.27 -5.51 -8.29 -9.20 4.64 8.50 -0.72 -5.00 -8.51
Bud Site -9.76 3.28 -10.25 -0.02 -11.85 -4.69 -3.19 -8.90 -7.50 -7.49 -9.75 0.33 -13.99 -7.73 -13.63
Cell Periphery -2.88 -2.43 0.34 3.00 2.87 -0.20 -7.08 -1.31 -0.95 5.41 -1.66 -7.29 -0.91 0.91 6.40
Cytoplasm 16.47 9.08 -20.21 -36.87 -29.05 9.19 -28.79 -39.22 -44.14 -10.60 20.15 -14.91 -40.35 -57.13 -20.80
Cytoplasmic Foci 26.63 29.44 41.56 15.22 9.66 18.17 17.69 36.65 28.51 15.86 33.04 33.07 50.91 23.99 15.23
Eisosomes 2.58 1.66 2.94 1.71 5.75 6.64 -4.65 14.32 11.01 4.98 3.89 -4.36 4.82 3.90 5.05
Endoplasmic Reticulum 1.98 -8.57 -4.62 -9.19 5.76 5.31 -4.18 4.44 -1.71 8.68 5.05 -9.26 -2.58 -11.09 8.21
Endosome -10.06 4.19 22.00 27.44 16.78 -8.08 19.07 23.93 32.97 9.47 -12.30 13.72 31.87 42.12 17.65
Golgi -16.83 10.25 15.59 23.03 6.14 -10.37 19.80 20.59 31.84 3.70 -20.26 21.78 24.81 38.98 6.44
Lipid Particles 7.46 -7.31 14.29 6.02 14.36 11.30 7.69 14.74 10.31 8.01 14.03 -1.71 20.06 10.50 15.75
Mitochondria -15.64 8.96 21.18 32.18* 12.60 -11.41 34.97* 33.74* 58.27* -3.33 -21.83 30.48 38.04* 65.93* 10.84
Mitotic Spindle -4.89 -7.77 -6.72 -3.36 3.05 -5.18 -5.01 -5.80 -3.65 -1.28 -7.14 -9.22 -8.87 -4.92 1.60
None -2.46 -6.12 -0.56 2.34 6.06 3.07 -3.86 -1.03 -5.47 3.58 1.48 -5.53 -0.13 -2.18 5.62
Nuclear Periphery 2.81 -9.62 -8.43 -9.90 8.41 3.46 -2.43 -0.22 -3.04 1.88 4.05 -9.73 -3.35 -8.23 8.50
Nuclear Periphery Foci -0.68 -6.80 -4.21 -3.98 5.44 2.45 -5.41 6.53 2.22 8.10 1.64 -7.76 -3.00 -3.66 6.39
Nucleolus -2.21 -12.61 3.94 4.21 13.67 4.19 1.68 4.66 0.66 2.78 2.16 -9.68 6.13 4.33 13.39
Nucleus 9.94 -22.39 -26.29 -32.86 5.42 9.34 -25.34 -21.87 -25.79 3.76 14.37 -33.48 -34.64 -42.67 6.88
Peroxisomes -5.32 6.89 4.26 8.21 -1.62 1.53 9.73 16.32 15.42 5.76 -2.25 11.86 11.00 12.14 0.31
Vacuole 3.86 -12.08 -25.06 -27.32 -10.01 -1.97 -12.21 -7.55 -6.44 5.27 0.86 -17.18 -25.54 -26.13 -6.63
Vacuole Periphery -6.73 -6.82 13.14 14.86 11.67 -1.43 13.86 14.36 21.48 2.08 -6.26 0.21 19.17 26.39 11.54
Standard name
Human Ortholog
Description Protein involved in incorporating iron-sulfur clusters into proteins; mitochondrial matrix protein; involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system
Localization
Cell Percentages mitochondrion (61%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Iba57

Iba57


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Iba57-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available