ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 3 | 0 | 1 | 2 |
Bud | 0 | 0 | 2 | 0 | 1 | 2 | 6 | 7 | 9 | 12 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 1 | 4 |
Bud Neck | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 1 | 1 | 2 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 7 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 6 | 5 | 6 | 0 | 0 | 2 | 0 | 1 | 1 |
Cytoplasm | 172 | 195 | 161 | 155 | 192 | 199 | 247 | 203 | 157 | 195 | 92 | 141 | 276 | 370 | 339 | 125 | 98 | 106 | 137 | 179 | 109 | 114 | 228 | 242 |
Endoplasmic Reticulum | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 6 | 5 | 3 | 2 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 2 | 1 | 3 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 2 | 0 | 0 | 5 | 4 | 2 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 2 | 3 | 0 | 0 | 0 |
Mitochondria | 34 | 7 | 2 | 11 | 15 | 58 | 123 | 89 | 98 | 157 | 145 | 158 | 1 | 1 | 2 | 6 | 2 | 1 | 35 | 11 | 42 | 2 | 6 | 7 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 4 | 0 | 2 | 3 | 5 | 6 | 3 | 8 | 1 | 0 | 1 | 1 | 0 | 0 | 4 | 2 | 2 | 0 | 1 | 4 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 2 | 5 | 0 | 0 | 2 | 7 | 2 | 5 | 3 | 2 | 0 | 0 | 2 | 0 |
Unique Cell Count | 189 | 203 | 169 | 159 | 199 | 225 | 313 | 260 | 226 | 290 | 201 | 249 | 280 | 375 | 346 | 137 | 103 | 114 | 202 | 213 | 178 | 124 | 254 | 272 |
Labelled Cell Count | 208 | 209 | 171 | 171 | 218 | 265 | 385 | 305 | 275 | 375 | 255 | 324 | 281 | 378 | 354 | 157 | 114 | 124 | 202 | 213 | 178 | 124 | 254 | 272 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.0 | 3.8 | 3.9 | 3.6 | 3.8 | 3.3 | 3.0 | 3.0 | 2.8 | 2.8 | 2.5 | 2.5 | 4.8 | 5.1 | 5.5 | 7.3 | 6.6 | 6.8 | 5.0 | 5.1 | 5.3 |
Std Deviation (1e-4) | 0.5 | 0.8 | 1.5 | 1.8 | 1.4 | 1.7 | 1.5 | 1.4 | 1.2 | 1.0 | 1.3 | 1.0 | 1.0 | 1.1 | 8.4 | 2.2 | 1.8 | 1.8 | 1.2 | 1.5 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.05 | -0.26 | -0.36 | -0.35 | -0.48 | -0.5 | -0.62 | -0.61 | 0.31 | 0.38 | 0.5 | 0.9 | 0.77 | 0.81 | 0.35 | 0.39 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8209 | 1.7452 | 1.7479 | 1.0695 | 1.4835 | 1.934 | -0.7963 | -0.3752 | -0.4423 | -0.8984 | -0.1527 | -0.448 | -1.5372 | -1.0037 | -1.3373 | -0.9787 | -1.4376 | -1.1302 |
Actin | 0.0354 | 0.0001 | 0.0006 | 0.0224 | 0.0057 | 0.0038 | 0.0217 | 0.0037 | 0.0124 | 0.0029 | 0.0219 | 0.0059 | 0.0261 | 0.0002 | 0.0128 | 0.0005 | 0.0082 | 0.0054 |
Bud | 0.0004 | 0.0001 | 0.0002 | 0.0193 | 0.0101 | 0.0003 | 0.0017 | 0.0035 | 0.0006 | 0.001 | 0.0014 | 0.0005 | 0.0056 | 0.0029 | 0.0002 | 0.0265 | 0.0004 | 0.0011 |
Bud Neck | 0.0017 | 0 | 0.0001 | 0.0002 | 0.0008 | 0.0008 | 0.0013 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0003 | 0.0009 | 0.0001 | 0.0041 | 0.0002 | 0.0004 | 0.0004 |
Bud Periphery | 0.0005 | 0.0001 | 0.0002 | 0.0166 | 0.0356 | 0.0003 | 0.0029 | 0.007 | 0.0007 | 0.0014 | 0.0017 | 0.0026 | 0.0039 | 0.001 | 0.0003 | 0.0141 | 0.0006 | 0.0024 |
Bud Site | 0.0024 | 0.0002 | 0.001 | 0.0037 | 0.0009 | 0.0001 | 0.0049 | 0.0058 | 0.0032 | 0.0003 | 0.002 | 0.0001 | 0.0033 | 0.0047 | 0.0045 | 0.0009 | 0.0013 | 0.0002 |
Cell Periphery | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0008 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.261 | 0.4242 | 0.4147 | 0.0642 | 0.3935 | 0.4156 | 0.1741 | 0.3486 | 0.3416 | 0.2894 | 0.2862 | 0.4471 | 0.2419 | 0.293 | 0.3127 | 0.2864 | 0.3005 | 0.3646 |
Cytoplasmic Foci | 0.0081 | 0.0039 | 0.0061 | 0.0172 | 0.0098 | 0.0049 | 0.0496 | 0.0085 | 0.0107 | 0.0431 | 0.0394 | 0.0114 | 0.0132 | 0.0052 | 0.0113 | 0.0173 | 0.0112 | 0.0135 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0145 | 0.0009 | 0.0028 | 0.0007 | 0.0044 | 0.0018 | 0.0057 | 0.0028 | 0.002 | 0.0117 | 0.0035 | 0.0018 | 0.0018 | 0.0007 | 0.0017 | 0.0019 | 0.0016 | 0.0015 |
Endosome | 0.01 | 0.0011 | 0.0016 | 0.0015 | 0.015 | 0.0019 | 0.0382 | 0.0036 | 0.0361 | 0.0282 | 0.0292 | 0.0072 | 0.0038 | 0.0009 | 0.0074 | 0.0037 | 0.0169 | 0.0053 |
Golgi | 0.0016 | 0.0001 | 0.0002 | 0.0023 | 0.0011 | 0.0003 | 0.0139 | 0.0004 | 0.0069 | 0.0032 | 0.0293 | 0.0017 | 0.0028 | 0.0001 | 0.0018 | 0.0002 | 0.0097 | 0.0008 |
Lipid Particles | 0.0009 | 0.0001 | 0.0003 | 0.003 | 0.0011 | 0.0004 | 0.0267 | 0.0004 | 0.0132 | 0.0036 | 0.012 | 0.0047 | 0.0038 | 0.0004 | 0.0016 | 0.0005 | 0.0091 | 0.0013 |
Mitochondria | 0.0014 | 0.0002 | 0.0002 | 0.0011 | 0.0031 | 0.0003 | 0.0089 | 0.001 | 0.018 | 0.0005 | 0.0115 | 0.0027 | 0.0014 | 0.0001 | 0.0017 | 0.0006 | 0.0054 | 0.001 |
None | 0.5889 | 0.5672 | 0.5688 | 0.8065 | 0.4894 | 0.5418 | 0.6014 | 0.6077 | 0.5409 | 0.5886 | 0.4781 | 0.5087 | 0.6781 | 0.6878 | 0.6337 | 0.6379 | 0.5993 | 0.5849 |
Nuclear Periphery | 0.0297 | 0.0002 | 0.0006 | 0.0006 | 0.0103 | 0.0152 | 0.0029 | 0.0008 | 0.0007 | 0.0043 | 0.0096 | 0.0008 | 0.001 | 0.0003 | 0.0009 | 0.0015 | 0.0068 | 0.0079 |
Nucleolus | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0.0008 | 0.0004 | 0.0008 | 0.0003 | 0.0001 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0028 | 0.0002 |
Nucleus | 0.0356 | 0.0005 | 0.0008 | 0.0033 | 0.0094 | 0.0035 | 0.0057 | 0.0014 | 0.0011 | 0.0027 | 0.0103 | 0.0014 | 0.0011 | 0.001 | 0.0012 | 0.0022 | 0.0072 | 0.0047 |
Peroxisomes | 0.0006 | 0 | 0.0002 | 0.0315 | 0.0008 | 0.0002 | 0.0182 | 0.0003 | 0.0068 | 0.0023 | 0.0031 | 0.0004 | 0.0069 | 0.0001 | 0.0018 | 0.0004 | 0.0016 | 0.0015 |
Punctate Nuclear | 0.0023 | 0.0002 | 0.0005 | 0.0026 | 0.0042 | 0.0074 | 0.0067 | 0.0008 | 0.0007 | 0.0124 | 0.0157 | 0.0007 | 0.002 | 0.0006 | 0.0007 | 0.0028 | 0.0142 | 0.0019 |
Vacuole | 0.0027 | 0.0006 | 0.0006 | 0.0019 | 0.0023 | 0.0004 | 0.012 | 0.0024 | 0.0027 | 0.003 | 0.0396 | 0.0013 | 0.0013 | 0.0007 | 0.001 | 0.0015 | 0.0014 | 0.0007 |
Vacuole Periphery | 0.0012 | 0.0001 | 0.0001 | 0.0003 | 0.001 | 0.0003 | 0.0021 | 0.0003 | 0.001 | 0.0007 | 0.0041 | 0.0005 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0011 | 0.0005 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.0712 | 7.3065 | 10.8435 | 14.3189 | 7.6535 | 28.1371 | 12.7231 | 13.5733 | 14.0257 | 13.4224 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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