Standard name | |
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Human Ortholog | |
Description | Adenylylsulfate kinase; required for sulfate assimilation and involved in methionine metabolism; human homolog PAPSS2 complements yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 3 |
Bud | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 3 | 3 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 3 | 3 |
Bud Neck | 0 | 1 | 2 | 0 | 1 | 2 | 3 | – | 1 | 8 | 3 | 5 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 4 | 2 | 0 | 0 | – | 0 | 0 | 2 | 5 | 0 | 0 | 0 | 1 | 6 | 1 | 2 | 1 | 3 | 0 | 0 | 0 |
Cytoplasm | 376 | 343 | 225 | 180 | 231 | 172 | 243 | – | 156 | 214 | 196 | 236 | 160 | 272 | 227 | 216 | 194 | 153 | 325 | 313 | 175 | 139 | 204 | 179 |
Endoplasmic Reticulum | 2 | 4 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 8 | 12 | 11 | 0 | 1 | 0 | 1 | 0 | 1 |
Endosome | 1 | 0 | 0 | 0 | 0 | 1 | 2 | – | 0 | 0 | 4 | 2 | 0 | 0 | 0 | 5 | 1 | 5 | 5 | 1 | 1 | 1 | 0 | 3 |
Golgi | 3 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 1 | 1 | 1 | 1 | 0 |
Mitochondria | 7 | 6 | 1 | 0 | 7 | 24 | 17 | – | 61 | 72 | 90 | 121 | 0 | 1 | 0 | 9 | 2 | 7 | 27 | 11 | 28 | 8 | 7 | 12 |
Nucleus | 15 | 0 | 0 | 1 | 1 | 1 | 2 | – | 4 | 3 | 2 | 1 | 4 | 0 | 1 | 4 | 6 | 6 | 15 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 3 | 4 | 2 | 8 | – | 0 | 11 | 8 | 15 | 1 | 0 | 0 | 2 | 2 | 0 | 7 | 1 | 1 | 1 | 3 | 2 |
Vac/Vac Membrane | 2 | 1 | 0 | 2 | 5 | 1 | 2 | – | 0 | 1 | 1 | 3 | 1 | 0 | 0 | 2 | 4 | 6 | 5 | 3 | 1 | 0 | 3 | 4 |
Unique Cell Count | 399 | 352 | 227 | 183 | 237 | 185 | 264 | 189 | 269 | 269 | 326 | 166 | 274 | 228 | 231 | 210 | 173 | 405 | 350 | 223 | 158 | 230 | 220 | |
Labelled Cell Count | 408 | 359 | 230 | 190 | 251 | 205 | 278 | 226 | 312 | 312 | 401 | 166 | 274 | 228 | 250 | 230 | 192 | 405 | 350 | 223 | 158 | 230 | 220 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 3.8 | 4.6 | 4.5 | 5.0 | 3.8 | 4.1 | – | 3.4 | 3.7 | 3.6 | 3.5 | 4.6 | 4.3 | 4.9 | 5.2 | 5.6 | 5.1 | 3.9 | 4.2 | 4.5 |
Std Deviation (1e-4) | 0.8 | 0.9 | 0.8 | 0.6 | 2.0 | 3.0 | 1.7 | – | 1.1 | 1.3 | 1.0 | 1.1 | 1.0 | 1.0 | 1.6 | 1.3 | 1.4 | 1.2 | 1.8 | 1.6 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.12 | -0.26 | -0.16 | – | -0.43 | -0.32 | -0.36 | -0.38 | -0.0 | -0.09 | 0.1 | 0.19 | 0.29 | 0.15 | -0.23 | -0.13 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0228 | -0.7385 | -0.7951 | -1.4489 | -0.9328 | -0.4836 | -0.0649 | 0.1501 | 0.2726 | 0.3396 | -0.2683 | 0.5282 | 1.849 | 3.6754 | 2.7149 | 3.3707 | 2.8454 | 3.0972 |
Actin | 0.1043 | 0.0011 | 0.0115 | 0.0007 | 0.0118 | 0.003 | 0.0342 | 0.0003 | 0.0006 | 0.0087 | 0.0071 | 0.0049 | 0.0131 | 0.0007 | 0.0005 | 0.0001 | 0.0027 | 0.0001 |
Bud | 0.0035 | 0.0005 | 0.0043 | 0.0012 | 0.0015 | 0.0004 | 0.0012 | 0.0014 | 0.0021 | 0.0039 | 0.0002 | 0.0006 | 0 | 0.0002 | 0.0027 | 0.0001 | 0.002 | 0 |
Bud Neck | 0.0042 | 0.0002 | 0.0008 | 0.0001 | 0.0004 | 0.0006 | 0.013 | 0.0003 | 0.0006 | 0.0003 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0003 | 0.0008 |
Bud Periphery | 0.0058 | 0.0009 | 0.006 | 0.0016 | 0.0045 | 0.0013 | 0.0032 | 0.0026 | 0.0025 | 0.012 | 0.0002 | 0.0023 | 0 | 0.0003 | 0.004 | 0.0001 | 0.0022 | 0.0001 |
Bud Site | 0.0109 | 0.0049 | 0.0052 | 0.0001 | 0.0019 | 0.0003 | 0.0046 | 0.0038 | 0.0103 | 0.0096 | 0.0002 | 0.0001 | 0.0004 | 0.0006 | 0.001 | 0 | 0.0003 | 0 |
Cell Periphery | 0.0005 | 0.0006 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0.0003 | 0.0002 | 0.0005 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0993 | 0.1311 | 0.1596 | 0.1434 | 0.191 | 0.1983 | 0.1261 | 0.2421 | 0.2055 | 0.1453 | 0.1283 | 0.2399 | 0.1001 | 0.19 | 0.1778 | 0.1097 | 0.2589 | 0.18 |
Cytoplasmic Foci | 0.0226 | 0.0103 | 0.0293 | 0.0069 | 0.0089 | 0.0067 | 0.0178 | 0.0052 | 0.0045 | 0.0088 | 0.0115 | 0.0096 | 0.0046 | 0.0042 | 0.0035 | 0.0015 | 0.01 | 0.0027 |
Eisosomes | 0.0008 | 0.0005 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0047 | 0.002 | 0.0021 | 0.0018 | 0.0039 | 0.002 | 0.0026 | 0.0009 | 0.0008 | 0.001 | 0.0023 | 0.0018 | 0.0004 | 0.0017 | 0.001 | 0.0006 | 0.0015 | 0.0005 |
Endosome | 0.0188 | 0.0022 | 0.0072 | 0.0016 | 0.024 | 0.0055 | 0.0105 | 0.0014 | 0.0012 | 0.0023 | 0.014 | 0.0066 | 0.0006 | 0.0008 | 0.0005 | 0.0002 | 0.0056 | 0.0004 |
Golgi | 0.0096 | 0.0004 | 0.0018 | 0.0002 | 0.0035 | 0.0006 | 0.003 | 0.0002 | 0.0004 | 0.0007 | 0.0019 | 0.0012 | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 |
Lipid Particles | 0.0092 | 0.0022 | 0.0046 | 0.0009 | 0.013 | 0.0011 | 0.0033 | 0.0002 | 0.0011 | 0.0005 | 0.003 | 0.0018 | 0.0086 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0 |
Mitochondria | 0.0031 | 0.01 | 0.0008 | 0.0012 | 0.0032 | 0.003 | 0.0023 | 0.0011 | 0.0079 | 0.0035 | 0.0007 | 0.0022 | 0.0007 | 0.0003 | 0.0003 | 0.0001 | 0.0038 | 0.0001 |
None | 0.6522 | 0.8265 | 0.7517 | 0.8312 | 0.6621 | 0.725 | 0.7362 | 0.6882 | 0.6791 | 0.6485 | 0.8182 | 0.6704 | 0.8312 | 0.7255 | 0.7718 | 0.8867 | 0.6925 | 0.7763 |
Nuclear Periphery | 0.0167 | 0.0008 | 0.0032 | 0.0021 | 0.0045 | 0.0254 | 0.004 | 0.0007 | 0.0008 | 0.0024 | 0.0039 | 0.0044 | 0.0002 | 0.0015 | 0.0005 | 0.0001 | 0.0031 | 0.0002 |
Nucleolus | 0.0011 | 0.0004 | 0.0005 | 0.0004 | 0.0005 | 0.0006 | 0.0011 | 0.0006 | 0.0007 | 0.0013 | 0.0002 | 0.0005 | 0.0005 | 0.0019 | 0.0008 | 0 | 0.0004 | 0.0005 |
Nucleus | 0.0104 | 0.0013 | 0.002 | 0.0017 | 0.0023 | 0.011 | 0.026 | 0.047 | 0.0748 | 0.1416 | 0.0011 | 0.0483 | 0.0322 | 0.0694 | 0.0307 | 0.0002 | 0.0084 | 0.0373 |
Peroxisomes | 0.0105 | 0.001 | 0.0029 | 0.0007 | 0.0381 | 0.0009 | 0.003 | 0.0007 | 0.0007 | 0.0024 | 0.0009 | 0.0008 | 0.0057 | 0.0002 | 0.0001 | 0 | 0.0006 | 0 |
Punctate Nuclear | 0.0083 | 0.002 | 0.0048 | 0.0023 | 0.0011 | 0.0118 | 0.0033 | 0.0009 | 0.0046 | 0.0011 | 0.0048 | 0.0024 | 0.0005 | 0.0012 | 0.0021 | 0.0001 | 0.0034 | 0.0005 |
Vacuole | 0.002 | 0.001 | 0.0009 | 0.0012 | 0.0198 | 0.0013 | 0.0027 | 0.002 | 0.001 | 0.0042 | 0.0006 | 0.001 | 0.0003 | 0.0006 | 0.0009 | 0.0002 | 0.0025 | 0.0002 |
Vacuole Periphery | 0.0013 | 0.0001 | 0.0002 | 0.0005 | 0.0036 | 0.0012 | 0.0008 | 0.0002 | 0.0002 | 0.0013 | 0.0003 | 0.0005 | 0 | 0.0002 | 0.0001 | 0 | 0.0011 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 61.6022 | 9.718 | 24.0372 | 77.9701 | 42.2841 | 38.0274 | 22.1082 | 32.0536 | 43.2717 | 41.1312 |
Translational Efficiency | 1.8929 | 3.8272 | 2.3356 | 1.1183 | 1.1805 | 2.8279 | 1.2797 | 0.9597 | 1.7173 | 1.517 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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