Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.85 0.87 0.94 0.88 – 0.75 0.5 0.65 0.44 0.48 0.99 1.0 0.98 0.94 0.96 0.98 0.89 0.79 0.55 0.88 0.79 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0.2 0.27 0.12 0.34 – 0.42 0.64 0.55 0.67 0.7 0 0 0 0.05 0.05 0.05 0 0.09 0.22 0 0.07 0.05
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 2 3
Bud 0 0 1 1 1 1 – 6 2 1 8 4 0 0 1 0 1 0 0 2 0 0 4 10
Bud Neck 0 0 0 0 0 1 – 3 0 0 0 1 0 0 0 0 0 0 0 3 0 0 3 4
Bud Site 0 0 0 0 0 0 – 1 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 1 0 0 – 0 0 0 0 2 0 4 1 4 0 1 0 1 0 1 3 2
Cytoplasm 205 235 35 81 104 113 – 179 74 101 53 76 358 445 524 159 94 42 183 189 33 140 284 394
Endoplasmic Reticulum 2 1 0 0 0 0 – 0 2 0 1 2 2 0 1 14 4 3 0 0 1 1 0 2
Endosome 0 0 0 1 1 0 – 0 0 0 0 0 0 0 3 2 1 0 3 3 3 0 3 4
Golgi 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 1 3
Mitochondria 14 3 8 25 13 44 – 102 94 86 80 111 2 2 3 8 5 2 3 21 13 7 23 23
Nucleus 0 0 0 0 0 0 – 1 1 3 1 0 0 1 2 2 1 1 0 0 0 0 1 0
Nuclear Periphery 0 0 1 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 0 – 1 1 0 0 1 0 0 0 1 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 – 7 1 4 3 4 0 0 1 0 0 0 0 2 0 2 9 10
Vac/Vac Membrane 1 3 0 1 1 1 – 1 2 2 3 3 1 1 1 3 3 0 5 7 2 1 8 11
Unique Cell Count 215 240 41 93 111 129 240 147 156 120 159 361 447 532 170 98 43 206 241 61 160 360 480
Labelled Cell Count 222 244 45 111 122 160 302 177 197 150 204 363 453 537 194 109 49 206 241 61 160 360 480


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.0 3.6 3.6 3.7 3.0 – 3.0 2.6 2.7 2.9 2.6 4.0 4.2 4.2 5.1 5.4 5.3 3.8 4.4 4.3
Std Deviation (1e-4) 0.6 1.0 1.6 2.4 1.9 1.7 – 1.6 1.4 1.4 2.3 1.4 1.4 1.4 1.8 1.6 1.7 1.3 1.4 2.2 1.9
Intensity Change (Log2) – – – 0.0 0.05 -0.25 – -0.28 -0.47 -0.4 -0.32 -0.46 0.16 0.22 0.23 0.51 0.59 0.55 0.1 0.28 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.937 -0.5454 -0.8903 -0.7881 -0.9739 -0.8548 -1.0931 -1.0632 -1.2465 -1.5174 -0.9163 -1.2853 1.4677 0.8031 0.2483 0.5336 0.8749 0.5629
Actin 0.0087 0.0015 0.0056 0.0428 0.003 0.0058 0.0168 0.0082 0.0037 0.0365 0.0426 0.005 0.1146 0.0008 0.0056 0 0.0598 0.0037
Bud 0.0009 0.0005 0.0003 0.0002 0.0004 0.0002 0.0008 0.0065 0.0013 0.0002 0.0037 0.0025 0.0012 0.0023 0.0035 0 0.0045 0.0001
Bud Neck 0.0057 0.0002 0.0006 0.0002 0.0003 0.0002 0.0023 0.0002 0.0006 0.0002 0.0009 0.0004 0.0049 0.0001 0.0003 0 0.0024 0.0004
Bud Periphery 0.0015 0.0005 0.0004 0.0002 0.0009 0.0007 0.0013 0.0078 0.003 0.0005 0.007 0.0019 0.0053 0.006 0.0079 0 0.0077 0.0004
Bud Site 0.0148 0.0028 0.0043 0.0013 0.0007 0.0001 0.011 0.0181 0.0037 0.0005 0.0177 0.0002 0.0028 0.0029 0.003 0 0.0134 0.0002
Cell Periphery 0.0005 0.0001 0.0002 0 0 0 0.0003 0.0004 0.0002 0 0.0004 0.0001 0.0006 0.0002 0.0001 0 0.0003 0
Cytoplasm 0.0136 0.0103 0.0129 0.0202 0.0114 0.0306 0.0215 0.0213 0.0319 0.009 0.0185 0.0518 0.0128 0.0391 0.0242 0.0134 0.0173 0.0496
Cytoplasmic Foci 0.033 0.0057 0.0137 0.0132 0.0332 0.0145 0.0461 0.0048 0.0162 0.0487 0.0155 0.0116 0.0227 0.0034 0.0024 0.001 0.0152 0.0023
Eisosomes 0.002 0.0004 0.0002 0.0002 0.0001 0.0001 0.0007 0.0004 0.0003 0.0001 0.0004 0.0001 0.0009 0.0002 0.0001 0 0.0007 0.0001
Endoplasmic Reticulum 0.0007 0.0003 0.0004 0.0003 0.0003 0.0003 0.0014 0.0014 0.0006 0.0001 0.0007 0.0012 0.0076 0.0003 0.0002 0.0001 0.001 0.0018
Endosome 0.0137 0.0003 0.0045 0.001 0.0093 0.0043 0.0283 0.0023 0.0138 0.0007 0.025 0.0028 0.0266 0.0003 0.0003 0 0.0109 0.0059
Golgi 0.0058 0.0003 0.0022 0.0017 0.0033 0.0026 0.008 0.0016 0.0066 0.0036 0.0142 0.0012 0.0182 0.0003 0.0004 0 0.0144 0.0012
Lipid Particles 0.0206 0.0026 0.0067 0.0069 0.0116 0.0061 0.0352 0.0005 0.0119 0.002 0.0348 0.0049 0.0254 0.0111 0.0003 0 0.0467 0.0003
Mitochondria 0.0047 0.0131 0.0017 0.0006 0.0046 0.0015 0.018 0.0011 0.0079 0.0021 0.0837 0.0079 0.0519 0.0545 0.0004 0 0.0063 0.0267
None 0.8438 0.9564 0.9358 0.8764 0.8972 0.9265 0.7614 0.9227 0.882 0.8804 0.6735 0.8952 0.6413 0.8752 0.949 0.9851 0.7248 0.8951
Nuclear Periphery 0.0014 0.0002 0.0005 0.0002 0.0005 0.0004 0.0027 0.0003 0.0005 0.0001 0.003 0.0034 0.0195 0.0001 0.0001 0 0.0031 0.0089
Nucleolus 0.0018 0.0003 0.0004 0.0001 0.0004 0.0001 0.0041 0.0003 0.0007 0.0001 0.001 0.0002 0.0006 0.0003 0.0003 0 0.0042 0.0001
Nucleus 0.001 0.0004 0.0004 0.0001 0.0004 0.0002 0.0033 0.0005 0.0006 0.0002 0.0008 0.0021 0.009 0.0003 0.0002 0 0.0024 0.001
Peroxisomes 0.0182 0.0027 0.0052 0.0334 0.0188 0.0047 0.0245 0.0005 0.0096 0.0122 0.0413 0.005 0.024 0.002 0.0009 0 0.0444 0.0001
Punctate Nuclear 0.0042 0.001 0.0024 0.0009 0.0026 0.0008 0.0081 0.0005 0.0032 0.0024 0.0115 0.0016 0.0052 0.0003 0.0007 0.0001 0.019 0.0006
Vacuole 0.0028 0.0003 0.0014 0.0002 0.0006 0.0003 0.0029 0.0006 0.0014 0.0001 0.0019 0.0004 0.0021 0.0003 0.0001 0 0.001 0.0004
Vacuole Periphery 0.0007 0.0001 0.0003 0 0.0003 0.0001 0.0013 0.0001 0.0003 0 0.0018 0.0005 0.0025 0.0001 0 0 0.0005 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.8978 27.3993 20.3315 35.1715 19.8696 25.0635 40.2959 51.8443 40.3717 41.6267
Translational Efficiency 1.7332 1.3228 1.3978 0.8319 1.7429 2.1121 1.3145 0.9947 1.1846 1.231

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1693 1156 1504 184 975 1754 815 1907 2668 2910 2319 2091

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 661.39 686.94 772.81 816.79 561.81 710.10 735.11 770.71 625.00 700.90 759.56 774.76
Standard Deviation 71.99 85.46 95.93 86.92 77.69 86.73 132.55 93.87 88.28 86.97 111.66 94.19
Intensity Change Log 2 — 0.054683 0.224613 0.304464 — 0.337940 0.387878 0.456106 — 0.191713 0.301900 0.376096

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000100 0.000864 0.000098 0.000173 0.000089 0.000281 0.000179 0.000133 0.000096 0.000512 0.000127 0.000136
Bud Neck 0.020228 0.036603 0.002366 0.003765 0.027170 0.056744 0.001898 0.006747 0.022765 0.048743 0.002202 0.006485
Bud Site 0.003386 0.011123 0.007620 0.006790 0.004660 0.011134 0.005531 0.014417 0.003852 0.011129 0.006886 0.013746
Cell Periphery 0.000112 0.000193 0.000087 0.000145 0.000593 0.000199 0.000156 0.000150 0.000288 0.000196 0.000111 0.000150
Cytoplasm 0.479014* 0.340404* 0.363527* 0.258841* 0.450770* 0.337126* 0.352712* 0.415801* 0.468693* 0.338428* 0.359726* 0.401990*
Cytoplasmic Foci 0.234590* 0.345894* 0.010996 0.024103 0.261910* 0.309586* 0.016010 0.023691 0.244574* 0.324010* 0.012758 0.023727
Eisosomes 0.000208 0.000248 0.000025 0.000063 0.000804 0.000298 0.000042 0.000042 0.000426 0.000278 0.000031 0.000044
Endoplasmic Reticulum 0.000539 0.000618 0.001403 0.003735 0.001077 0.000642 0.001647 0.000917 0.000736 0.000633 0.001489 0.001165
Endosome 0.009272 0.022695 0.001221 0.003450 0.006179 0.036486 0.001731 0.003863 0.008142 0.031007 0.001401 0.003826
Golgi 0.002226 0.006897 0.000041 0.000058 0.003997 0.008510 0.000108 0.001142 0.002873 0.007869 0.000065 0.001047
Lipid Particles 0.008670 0.013465 0.000912 0.003017 0.029824 0.015388 0.001279 0.001285 0.016400 0.014624 0.001041 0.001437
Mitochondria 0.005090 0.017888 0.000356 0.000441 0.021676 0.020671 0.001120 0.001464 0.011151 0.019565 0.000624 0.001374
Mitotic Spindle 0.001121 0.001868 0.001092 0.000157 0.000333 0.002452 0.003353 0.014432 0.000833 0.002220 0.001887 0.013176
None 0.002956 0.002711 0.005155 0.003758 0.007522 0.002174 0.010895 0.002625 0.004625 0.002387 0.007172 0.002725
Nuclear Periphery 0.000319 0.000313 0.001152 0.001787 0.000477 0.000362 0.001059 0.001552 0.000377 0.000342 0.001120 0.001573
Nuclear Periphery Foci 0.000144 0.000418 0.000390 0.001134 0.000441 0.000249 0.000515 0.000283 0.000252 0.000316 0.000434 0.000358
Nucleolus 0.000772 0.001659 0.000447 0.000955 0.001728 0.001635 0.000859 0.000655 0.001121 0.001645 0.000592 0.000681
Nucleus 0.183463* 0.112703 0.582699* 0.622696* 0.156194* 0.105264 0.565275* 0.450609* 0.173498* 0.108219 0.576576* 0.465752*
Peroxisomes 0.005739 0.035365 0.000156 0.000512 0.009361 0.024822 0.000336 0.000907 0.007063 0.029010 0.000219 0.000873
Vacuole 0.041391 0.046160 0.020138 0.064097 0.013902 0.062997 0.033961 0.058473 0.031345 0.056309 0.024996 0.058968
Vacuole Periphery 0.000659 0.001913 0.000116 0.000320 0.001291 0.002980 0.001335 0.000811 0.000890 0.002556 0.000545 0.000768

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.05 -1.65 -3.17 1.80 -2.15 -5.09 -2.84 -2.97 3.50 1.27 -2.78 -3.26 -3.35 2.42 0.07
Bud Neck -5.58 12.90 10.21 11.98 -0.87 -7.54 9.15 7.28 17.01 -6.37 -10.83 15.36 11.28 20.04 -6.33
Bud Site -6.38 -2.03 -0.62 1.90 0.48 -4.25 0.02 -3.66 -0.31 -3.58 -8.06 -1.74 -4.82 -0.01 -2.79
Cell Periphery -5.70 3.08 -1.10 2.46 -2.23 9.04 10.10 10.66 4.79 1.12 5.03 10.27 8.24 5.26 -1.21
Cytoplasm 14.01 14.39 12.27 4.70 4.89 11.43 10.81 7.28 -4.48 -5.29 18.94 17.94 13.03 -3.57 -3.97
Cytoplasmic Foci -12.57 42.17 31.55 38.98 -2.87 -5.75 38.20 37.37 51.27 -3.54 -13.37 56.39 52.76 66.79 -6.76
Eisosomes -2.26 13.83 9.48 12.93 -5.99 7.31 11.22 11.28 15.02 1.00 5.15 14.71 14.26 20.45 -5.72
Endoplasmic Reticulum -0.69 -9.90 -6.81 -6.64 -4.17 1.82 -4.32 -1.02 -5.66 5.32 0.90 -9.77 -6.83 -8.97 3.92
Endosome -8.03 10.27 6.22 12.19 -3.68 -14.96 8.53 4.91 16.64 -3.22 -16.01 12.64 7.96 20.25 -4.58
Golgi -6.44 11.65 11.55 9.83 -2.43 -5.60 11.23 6.11 9.37 -1.62 -9.12 16.10 5.38 11.30 -1.70
Lipid Particles -3.53 12.01 7.07 8.02 -3.12 6.11 13.46 13.52 13.36 0.69 1.44 17.05 16.72 16.45 -2.65
Mitochondria -5.91 6.52 6.45 8.59 -0.25 0.37 8.41 8.32 12.39 -0.93 -5.20 10.35 9.70 14.74 -3.19
Mitotic Spindle -0.78 -0.05 1.68 3.24 2.20 -3.49 -2.17 -6.78 -5.48 -4.33 -2.51 -1.69 -6.36 -5.62 -5.61
None 0.18 -3.24 -0.68 -0.76 2.05 5.25 -0.60 5.00 -1.29 4.74 3.87 -2.18 3.95 -0.69 6.94
Nuclear Periphery 0.09 -16.65 -8.26 -8.32 -4.33 1.58 -10.93 -15.46 -16.57 -5.25 0.73 -19.76 -19.02 -19.30 -7.91
Nuclear Periphery Foci -1.67 -7.60 -5.48 -3.69 -3.91 1.32 -0.15 1.06 -1.22 4.03 0.02 -2.29 -1.05 -1.45 2.60
Nucleolus -3.41 5.80 0.10 2.99 -1.95 0.32 2.73 3.23 6.47 1.40 -2.69 4.23 3.66 7.03 -0.63
Nucleus 11.30 -47.87 -18.22 -21.30 0.38 8.09 -35.61 -33.39 -44.14 10.26 15.12 -59.71 -38.46 -48.56 13.86
Peroxisomes -12.11 11.18 10.54 14.69 -2.96 -8.86 11.21 10.35 15.34 -2.26 -15.57 15.78 13.47 21.00 -2.93
Vacuole -1.50 5.50 -5.21 -4.86 -6.26 -19.40 -10.02 -21.65 -8.48 -8.31 -11.88 -2.65 -17.85 -11.19 -15.43
Vacuole Periphery -4.97 9.18 4.35 6.46 -1.97 -4.53 0.46 3.39 6.19 0.52 -6.95 1.85 2.04 7.19 -0.47
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator
Localization
Cell Percentages cytoplasm (13%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mrp17

Mrp17


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrp17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available